ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
·
IF 4.527
Lin Li,Xianwei Shen,Chaocheng Zhao,Qiyou Liu,Xuwei Liu,Yanan Wu
A potent bacterial strain capable of degrading dibenzothiophene (DBT) was isolated and evaluated for its characteristics. The strain, designated as LKY-5, is rod-shaped, gram-negative, and occurs mainly in clusters. It was identified as belonging to the Pseudomonas genus based on the 16S rDNA sequence and phylogenic analysis. Determination of its DBT depletion efficiency by gas chromatography revealed that the isolate was able to completely degrade up to 100 mg L −1 DBT within 144 h. The pH values, DBT concentrations, and biomasses in the medium varied significantly in the initial 24 h. A biosurfactant produced by LKY-5 was extracted and identified as a di-rhamnolipid with the formula Rha-Rha-C 8 -C 8:1 by HPLC-ESI-MS/MS. There were 26 metabolites in the DBT degradation process. Pseudomonas sp. LKY-5 exhibited unusually high DBT degradation efficiency via multiple metabolic pathways. Compared with the reported 4S and Kodama pathways, two more expanded metabolic pathways for the degradation of DBT are proposed. The polycyclic aromatic sulfur heterocycles (PASHs) in diesel , such as C 1 -DBT, C 2 -DBT, C 3 -DBT, 4,6-DMDBT, and 2,4,6-TMDBT, can also be degraded with 28.2–42.3% efficiency. The results showed that LKY-5 is an excellent bacterial candidate for the bioremediation of PASH-contaminated sites and sediments.
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高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Mengyan Zeng,Junyan Tan,Kun Chen,Dejin Zang,Yang Yang,Jie Zhang,Yongge Wei
The two-dimensional (2D) nanostructures comprised of polyoxometalate based building blocks are of great value in nanoarchitectures, which have unique properties and widespread potential applications, but it is still challenging in mature preparation. Herein a new strategy to build Cr(III) centered Anderson type polyoxometalate 2D nanostructures based on the modulation of host–guest interaction between cationic pillar[5]arenes and sodium dodecyl sulfonate (SDS) in aqueous media was exploited in this work. Through regulating stoichiometry of SDS, the morphology of assemblies vary from nanobones to 2D nanosheets. The fine assembled structure was discovered by combined 1H NMR, SAXS, and element analyses. The nanomaterials can be used as adsorbents for I2 in various solutions, including n-hexane, cyclohexane, water, and chloroform, where the polyoxometalates play a key role in the effective adsorption of iodine since they can expand the interspace between pillar[5]arenes in the as-prepared nanostructure. Furthermore, such adsorbents are easily regenerated and reused as iodine can be released spontaneously from nanobones@I2 and nanosheets@I2 solids when being immersed in dimethyl sulfoxide.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
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2019
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IF 2.142
Rong Xiong,Wei Zhao,Xi Chen,Tian Li,Haiyin Li,Yan Li,Wangshang Shen,Ping Chen
Serotonin is involved in the regulation of many physiological and behavioral processes in vertebrates and invertebrates. The effects of serotonin are mediated through interactions of several 5-HT receptor types. The expression and pharmacological properties of 5-HT1 have received more attention than other serotonin receptors, but its functions at the individual level are little studied in arthropods. Silkworm , a Lepidoptera model, almost has no reports about serotonin receptors. To analyze the function of Bm5-HT1A receptor in vitro , the ORF of Bm5-HT1A was cloned into the pcDNA3.1 vector and expressed in HEK 293 cells . Serotonin activation of Bm5-HT1A -expressing cells decreased forskolin-stimulated cAMP synthesis and had the most potent effect compared to other biogenic amines. Serotonin reduced cAMP synthesis in a dose-dependent manner, and half-maximal activation (EC50) occurred at a concentration of 1.17 × 10 −7 M (117 nM). The pharmacological analysis demonstrated that the rank potency of agonists was pimozide >8-OH-DPAT >5-MeOT ~ αm-5-HT, and antagonists was WAY-100635 > prazosin > SB-269970 > methiothepin at the Bm5-HT1A receptor. Injecting the antagonist of Bm5-HT1A receptor into larvae caused slow or weak motility, and adults lowered courtship vitality or moving speed. Injecting dsRNA of Bm5-HT1A into adults also dropped locomotivity in courtship. These results show that the Bm5-HT1A receptor is related to locomotor activity. This study provides the first information of serotonin receptor on pharmacological in silkworm and on individual functions in arthropods.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
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IF 4.61
Guanyi Chen,Ruixue Zhang,Wenchao Ma,Bin Liu,Xiangping Li,Beibei Yan,Zhanjun Cheng,Tiejun Wang
The catalytic cracking upgrading reactions over HZSM-5 of different model compounds of bio-oil have been studied with a self-designed fluid catalytic cracking (FCC) equipment. Typical bio-oil model compounds, such as acetic acid, guaiacol, n-heptane, acetol and ethyl acetate, were chosen to study the products distribution, reaction pathway and deactivation of catalysts. The results showed: C 6 –C 8 aromatic hydrocarbons , C 2 –C 4 olefins , C 1 –C 5 alkanes, CO and CO 2 were the main products, and the selectivity of olefins was: ethylene > propylene > butylene . Catalyst characterization methods, such as FI-IR, TG-TPO and Raman, were used to study the deactivation mechanism of catalysts. According to the catalyst characterization results, a catalyst deactivation mechanism was proposed as follows: Firstly, the precursor which consisted of a large number of long chain saturated aliphatic hydrocarbons and a small amount C C of aromatics formed on the catalyst surface. Then the active sites of catalysts had been covered, the coke type changed from thermal coke to catalytic coke and gradually blocked the channels of the molecular sieve, which accelerated the deactivation of catalyst.
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高影响因子
Catalysis Science & Technology
·
2018
·Vol. 8
·期 17
·4430-4439
·
IF 5.365
Ziliang Yuan,Bing Liu,Peng Zhou,Zehui Zhang,Quan Chi
The selective oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-diformylfuran (DFF) has attracted much attention in recent years. Cesium-doped manganese dioxide (Cs/MnOx) was prepared by a soft template method, and was found to be active for the selective oxidation of HMF to DFF with molecular oxygen. Various reaction conditions have been investigated, and DFF was attained with a high yield of 94.7% at a HMF conversion of 98.4% at 100 °C and 10 bar O2 in N,N-dimethylformamide. Cs/MnOx demonstrated much higher catalytic activity than un-doped MnOx, suggesting that the introduction of Cs into MnOx plays a crucial role in the oxidation of HMF to DFF. Kinetic studies demonstrated that the oxidation of HMF to DFF depends on both the HMF and oxygen concentration, with the reaction order being 0.44 and 0.53, respectively. Compared with other heterogeneous non-noble metal catalysts, Cs/MnOx demonstrated a much lower activation energy for the oxidation of HMF to DFF. The catalyst can be reused without much loss of catalytic activity, suggesting that the catalyst is highly stable. Furthermore, the direct production of HMF from fructose was also successfully realized by a one-pot reaction strategy via two consecutive steps.
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Catalysis Science & Technology
·
2018
·Vol. 8
·期 21
·5526-5534
·
IF 5.365
Songyan Jia,Xinjun He,Jiao Ma,Kangjun Wang,Zhanwei Xu,Z. Conrad Zhang
The synthesis of furan compounds by renewable carbohydrates has emerged as an important path toward biomass utilization. 5-Hydroxymethylfurfural (5-HMF) and furfural are two typical furan products from cellulosic and hemicellulosic materials, of which the primary components (hexoses and pentoses) undergo selective dehydration. It is worth noting that hemicellulose is rich in not only pentoses but also hexoses, indicating the feasibility of producing 5-HMF and furfural simultaneously. However, research on the production of 5-HMF from hemicellulose-derived hexoses remains lacking. Mannose, a major hexose component of hemicellulose, has been previously found to be a suitable feedstock for 5-HMF production in the presence of homogeneous catalysts, such as metal salts. In this work, Cr-, Al- and Sn/MCM-41 catalysts were prepared by a simple impregnation method and characterized. The conversion of mannose into 5-HMF was evaluated in dimethyl sulfoxide (DMSO) solvent. It was found that the as-synthesized Sn/MCM-41 catalyst showed a superior activity to Cr/MCM-41 and Al/MCM-41. Mannose could be effectively converted into 5-HMF with a yield of ∼45% and ∼88% conversion at 150 °C after 60 min, which were comparable to reported results over heterogeneous catalysts. The as-synthesized Sn/MCM-41 catalyst was also efficient for the conversion of glucose and fructose with reasonable 5-HMF yields. The Sn/MCM-41 catalyst could be reused for eight consecutive cycles without significant loss of catalytic activity.
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ACS Omega
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2018
·
IF 0
Changhui Zhu,Qiying Liu,Dan Li,Haiyong Wang,Caihong Zhang,Chunhua Cui,Lungang Chen,Chiliu Cai,Longlong Ma
Crystal α-zirconium phosphate (α-ZrP) was prepared by a hydrothermal method and exfoliated into a layered structure by n-hexylamine (C6H13NH2). Ni-based catalyst (Ni/ZrP) was promoted by loading nickel on the layered α-ZrP via ion exchange. The catalyst was performed to catalyze hydrodeoxygenation of 5-hydroxymethylfurfural (HMF) to 2,5-dimethylfuran (DMF), and a 68.1% yield of DMF and 100% conversion of HMF were achieved at 240 °C, 5 MPa H2, and 20 h. The DMF yield can still retain 52.8% after five cycles. The characteristics of the catalyst were investigated via N2 adsorption–desorption, X-ray diffraction, field emission scanning electron microscopy, high-resolution transmission electron microscopy, pyridine-adsorbed Fourier transform infrared (FTIR) spectra, FTIR spectra, inductively coupled plasma mass spectrometry, and thermogravimetric analysis–mass spectrometry, as well as Raman spectroscopy. A pathway from HMF to DMF was found with MF as the intermediate product, and DMF production was preferable via the −CH2OH group hydrogenolysis of HMF over Lewis acidic sites of Ni/ZrP, which is caused by the zirconium vacant orbits.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2018
·Vol. 34
·期 4
·661-664
·
IF 1.248
Yi Ting,Li Jingwei,Zhang Yibo,Yang Xiangguang
Hollow-ZSM-5 was synthesized by a traditional hydrothermal method without alcohol, and a novel na-no-sized Ce-Cu/hollow ZSM-5 sample was prepared by conventional incipient wetness impreganation. The catalysts were characterized by TEM, SEM, XRD, NMR and N 2 adsorption-desorption. The results indicate that the hollow-ZSM-5 has a good MFI structure with large surface area. The synthesized CeO 2 -CuO/hollow ZSM-5 showed an excellent catalytic activity for NO x conversion(90% NO x conversion were obtained) in the temperature range of 340–550 °C under GHSV=480000 mL·g ‒1 ·h ‒1 due to its appropriate redox ability and acidity.
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SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Zhenping Liu,Mingliang Jin,Jieping Cao,Ruiwen Niu,Pengfei Li,Guofu Zhou,Ying Yu,Albert van den Berg,Lingling Shui
Inspired by the trend of device miniaturization for quick and sensitive detection with small amount of samples in biomedical and forensic fields. An electrochemical sensor based on poly (diallyl dimethylammonium) chloride - reduced graphene oxide (PDDA-RGO) modified multi-walled carbon nanotubes - carbon paste electrode (MWCNT-CPE), abbreviated as MWCNT-CPE/PDDA-RGO, was fabricated and integrated with microfluidic device for microliter sample detection. Detailed characterizations reveal that hybrid nanomaterials of PDDA-RGO and MWCNT have a synergistic effect for the electrocatalytic reaction of dopamine and 5-hydroxytryptamine, enabling detection with high sensitivity. Such an integrated microfluidic device exhibits excellent sensitivity, wide linear range, and good stability and repeatability for 20 μL sample detection. The calibration curves obtained by voltammetry show the linear range of 0.05–120.0 μM with limit of detection (LOD) of 0.016 μM (S/N = 3) for dopamine, and 0.05–50.0 μM with LOD of 0.0098 μM (S/N = 3) for 5-hydroxytryptamine. Its capability of sensitively and selectively quantify dopamine and 5-hydroxytryptamine in microliter volume rat’s plasma, suggests that, it can be potentially applied in monitoring bioactive substances both in vivo and in vitro .
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Qiuyue Sha,Bingyang Sun,Chen Yi,Ruixue Guan,Jian Fei,Zhaoyu Hu,Bifeng Liu,Xin Liu
In the past few decades, protein-templated fluorescent gold nanoclusters have attracted much attention in the field of biosensing as their excellent optical properties and good biocompatibility. In this work, a fluorescence turn-on biosensor was fabricated for the sensitive detection of 5-hydroxytryptamine (5-HT) with transferrin encapsulated gold nanoclusters (Tf-Au NCs). The Tf-Au NCs were facilely prepared with the assistance of microwave radiation in several minutes and exhibited good monodispersity as well as strong red-emission. It was demonstrated that an aggregation-enhanced emission of Tf-Au NCs was triggered by 5-HT due to the high affinity between 5-HT and the sialic acid (SA) residues of transferrin. Based on these findings, a highly selective turn-on fluorescent sensor was fabricated for the quantitative determination of 5-HT in the range of 0.2–50 μM (R 2 = 0.994) with a detection limit of 0.049 μM (S/N = 3). The developed sensor was also successfully applied for the 5-HT detection in human serum with satisfactory recoveries of 96.20–108.6%.
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TALANTA
·
2018
·
IF 4.244
Li Yang,Yonghai Song,Mulan Zeng,Yue Du,Bingxian Peng,Zhenzhong Huang,Li Wang
It is of great significance for superoxide anion (O 2 •− ) detection due to its important role in regulation of biological function. The nanoscale coordination polymers (NCPs) core-shell nanostructures have attracted increasing interests for their nanosized-dependent properties. In this study, SiO 2 @Tb/guanosine 5′-monophosphate (GMP) NCPs with core-shell nanostructures was designed for O 2 •− detection. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were produced by using SiO 2 nanoparticles (NPs) as template to control their nano-size. First, Tb 3+ was adsorbed on the surface of SiO 2 NPs by coordination with -COOH of SiO 2 NPs. Then the Tb/GMP NCPs shells were grown along the surface of SiO 2 NPs. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and Zeta potential. The resulted SiO 2 @Tb/GMP NCPs core-shell nanostructures displayed strong Tb/GMP emission which decreased linearly with the increased O 2 •− concentration in the range from 5.3 nM to 6.0 μM with a limit detection of 2.18 nM. And the SiO 2 @Tb/GMP NCPs core-shell nanostructures also exhibited good selectivity and high sensitivity in HEPES buffer at pH 7.4.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Zhe Wen,Linhao Yu,Fuhang Mai,Zewei Ma,Hong Chen,Yongdan Li
The catalytic conversion of microcrystalline cellulose is examined over a niobic acid catalyst (NBO) prepared by an alkali fusion method. The catalytic activity of NBO is significantly affected by the reaction conditions and further acid treatment. The highest 5-hydroxymethylfurfural (HMF) yield of 7.0 mol % and a glucose yield of 17.7 mol % at a cellulose conversion of 54.2% are achieved at 230 °C for 2 h. The H2SO4-treated (NBS) and H3PO4-treated (NBP) samples both show greatly improved performance over NBO due to the introduction of strong acid sites and the increase in specific surface area. NBS gives a glucose yield of 26.0 mol % and an HMF yield of 14.1 mol %, while NBP exhibits a glucose yield of 21.4 mol % and an HMF yield of 15.5 mol %. NBO shows excellent recyclability for the catalytic hydrolysis of cellulose. The recovered NBS and NBP samples can be regenerated by calcination and subsequent acid treatment.
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危险品化学品经营许可证（带存储）