JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Gong Jing,Zhou Min,Huang Yue,Yu Mengjun,Zhou Qiang,Chen Shiwen,Wang Jia,Yang Min,Luo Qing,Zhang Rong,Huang Jingbin
Asthma is a chronic inflammatory respiratory disease worldwide. Its severe phenotypes, particularly the glucocorticoid-resistant ones, pose a major challenge, with neutrophilic inflammation being the key driver. However, no effective treatments are currently available. Herein, we report a macromolecular therapeutic strategy capable of targeting multiple key cells involved in the pathogenesis of neutrophilic asthma. The macromolecular therapy named PMART is constructed by conjugating artesunate and polyethylene glycol to the triazine scaffold. It is highly water-soluble and can self-assemble into nanomicelles, which enables efficient targeting to inflamed lung tissues and localization in inflammatory cells. In a murine model of neutrophilic asthma, PMART has proven to have in vivo efficacy equivalent to or greater than that of two clinically used anti-asthma drugs. Mechanistically, PMART reduces succinate levels by inhibiting branched-chain amino acid transaminase 1 (BCAT1) overexpression, thereby suppressing neutrophilic inflammation via blocking the NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome and the production of pro-inflammatory cytokines. Notably, PMART exhibits a favorable safety profile in mice. Taken together, these data support the potential of PMART as a novel and highly effective therapeutic agent for neutrophilic asthma and other neutrophil-mediated diseases. Graphical
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Nature Communications
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2026
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IF 18.1
Shakeri Amid,Abdo Dhana,Lei Matthew Ho Cheong,Okhovatian Sargol,Beeghly Garrett F.,Popovic Anna Maria,Jiang Richard,Liu Chuan,Wagner Karl T.,Vosoughi Daniel,Kieda Jennifer,Fish Jason E.,Radisic Milica
Despite the systemic spread of cancer, the ventricular myocardium is one of the least common sites of metastasis—a phenomenon that remains poorly understood. To examine this, we develop the Multi-organ Invasion Device (MInD), an organ-on-a-chip platform that enables multi-organ culture under flow. Organ compartments are connected in MInD using PermeoTubes—3D-printed porous conduits that support cancer cell intravasation, migration, and extravasation. In dual-organ devices, where highly aggressive breast cancer cells are co-cultured with either hepatic or cardiac tissue, invasion into cardiac tissue is significantly suppressed relative to hepatic co-culture. Importantly, in cardiac–hepatic–cancer tri-culture, the presence of cardiac tissue reduces overall invasion, with cancer cells preferentially migrating toward hepatic compartments. Cytokine profiling and RNA sequencing reveal that cardiac co-culture suppresses cell metastasis and invasion, while inducing immune activation. Overall, this platform presents an approach for uncovering organ-specific drivers of metastasis, accelerating future discovery of metastasis-inhibiting therapies.
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MOLECULAR BIOLOGY REPORTS
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2026
·Vol. 53
·期 1
·1218
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IF 3.2
Li Feilong,Jiang Yingcun,Zhou Yang,Song Chao,Liu Zongchao,Fu Zhijiang
Osteoporosis is a major public health concern characterized by reduced bone mass and increased fracture risk, and Cyathulae Radix has been used in China for its prevention, yet its active constituents and mechanisms remain unclear. This study integrated network pharmacology with in vitro experiments to identify key bioactive components and underlying pathways. Using the TCMSP database, we screened the major compounds of Cyathulae Radix and, through intersection with osteoporosis-related targets from OMIM and GeneCards, obtained 29 overlapping genes. Subsequent protein–protein interaction network analysis via STRING revealed core targets including AKT1, TNF, IL-6, and MMP2 , among which quercetin emerged as a principal active component. To validate its effects, we employed H₂O₂ to induce oxidative damage in MC3T3 E1 pre-osteoblasts, and then treated cells with quercetin based on CCK8 and ROS assay results. The CCK8 assay showed that quercetin restored cell viability to near-normal levels. Quercetin treatment significantly improved cell proliferation and restored osteogenic differentiation, as shown by elevated alkaline phosphatase staining intensity, increased mineralized nodules in alizarin red staining, and markedly upregulated mRNA levels of RUNX2, OPN , and BMP-2 . Furthermore, quercetin attenuated oxidative stress by increasing SOD and GSH activities, decreasing MDA production, and activating the NRF2/HO-1/GPX4 antioxidant cascade, as confirmed by western blotting. Collectively, these results demonstrate that quercetin, a key constituent of Cyathulae Radix, exerts anti osteoporotic effects through both promoting osteogenesis and enhancing antioxidant defense, offering a mechanistic rationale for its clinical use against osteoporosis.
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MICROCHIMICA ACTA
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2026
·Vol. 193
·期 8
·525
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IF 5.4
Tang Yafang,Zhang Liwen,Wang Wenjing,Yang Xing
A novel biosensing platform is proposed that integrates catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cascade isothermal amplification with the CRISPR/Cas12a system, enabling ultrasensitive detection of microRNAs (miRNAs) targets. Within this platform, two modules are integrated: a target recognition and signal amplification module constructed by the cascade of CHA and HCR, and a signal transduction module in which the CRISPR/Cas12a system acts in concert with DNA probes loaded onto gold nanoparticles (AuNPs). This design achieves cascaded amplification from target recognition to signal output, thereby conferring high signal gain. Experimental results demonstrate that the proposed biosensor had high sensitivity toward the target miRNA, with a detection limit as low as 37 fM. Moreover, it shows remarkable single-base discrimination capability, effectively distinguishing sequences with single-nucleotide mismatches. Notably, the sensor maintains stable and reliable performance in complex biological matrices, including serum samples and lysates from various tumor cells. This strategy effectively couples signal amplification with the CRISPR system, achieving both high sensitivity and specificity, making it a useful tool for miRNA detection and early cancer screening. Graphical
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Biology Direct
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2026
·Vol. 21
·期 1
·113
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IF 5.5
Wang Wei,Hu Yuanyuan,Xu Yan,Ding Ning,Wei Jiping,Li Cairong,Chen Yong
Background Doxorubicin (DOX) is a widely used broad-spectrum chemotherapy drug, but its severe organ toxic and side effects limit its clinical application. Tetrahydropalmatine (THP) protects against DOX-induced renal injury, yet its underlying mechanism remains unclear. This study aimed to verify THP’s protective effect and explore if it acts via the SIRT3-mediated Nrf2/HO-1 signaling pathway. Methods This study adopted an integrated in vivo and in vitro research system to explore the relevant mechanisms. In vivo, a DOX-induced mouse renal injury model was established with THP intervention; renal function was evaluated by detecting serum creatinine (Scr) and blood urea nitrogen (BUN), while renal injury severity was assessed via urine microalbumin (mALB) and urine albumin/creatinine ratio (UACR). Renal histopathological changes including glomerular injury and tubulointerstitial fibrosis were observed, and subcellular structures such as podocyte integrity and mitochondrial morphology were analyzed by transmission electron microscopy (TEM); additionally, the expression of pathway-related proteins was detected. In vitro, a DOX-induced cell injury model was constructed using mouse podocytes (MPC-5), and Western blot and immunofluorescence techniques were employed to determine the expression of key molecules in the Sirt3-Nrf2/HO-1 pathway, combined with in vivo and in vitro experiments to clarify the underlying mechanism. Results THP significantly attenuated DOX-induced renal injury, improved renal function (reduced Scr/BUN), mitigated histopathological/mitochondrial abnormalities, and preserved podocyte integrity. Molecularly, THP upregulates podocyte markers Nephrin/Podocin, enhances antioxidant capacity (increased GSH, SOD, and CAT, decreased MDA), inhibits inflammation (downregulated IL-6, IL-1β, and TNF-α), and suppresses apoptosis (downregulated Bax/caspase-3, upregulated Bcl-2). Notably, both in vivo and in vitro data link THP’s protection to SIRT3-mediated Nrf2/HO-1 pathway activation. Conclusion THP protects against DOX-induced renal injury in mice. Mechanistically, this may involve activating the SIRT3-mediated Nrf2/HO-1 pathway, improving podocyte function, and inhibiting oxidative stress, inflammation, and apoptosis.
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EMBO JOURNAL
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2026
·Vol. 45
·期 13
·4492-4530
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IF 8.4
Zhang Yuting,Sun Jun,Cai Yang,Xu Ziyan,Li Xiang,Wei Wei,Liu Pai,Sun Qiming,Wang Zhi-Hao,Cui Yixian
Endoplasmic reticulum autophagy (ER-phagy) is a selective autophagy pathway in which receptor proteins target ER membranes and proteins for degradation, yet its role in Alzheimer’s disease (AD) remains unclear. Here, we identify FAM134B/RETREG1 as a specific ER-phagy receptor mediating amyloid precursor protein (APP) degradation. FAM134B directly interacts with ER-localized wild-type and familial mutant APP via their C-terminal domains and recruits LC3 through its LC3-interacting region (LIR) to promote APP delivery to phagophores for lysosomal degradation. In AD, epigenetic silencing at the FAM134B promoter suppresses its transcription by limiting TFEB/TFE3 binding despite their nuclear enrichment. This transcriptional suppression impairs ER-phagy, leading to APP accumulation and exacerbated AD pathology. AAV-mediated hippocampal expression of wild-type, but not LIR-mutant, FAM134B in 5XFAD mice restores ER-phagy, enhances APP clearance, reduces Aβ deposition, preserves synaptic and myelin integrity, and improves cognitive performance. These findings establish FAM134B downregulation as an upstream pathogenic event in AD, suggesting ER-phagy enhancement as a promising strategy to suppress Aβ generation at its source.
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Nature Communications
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2026
·
IF 18.1
Zhang Weiluo,Zhang Yilei,Wan Da,Gao Junrong,Zheng Shuling,He Jie,Cai Huizhu,Zhang Xue,Hu Qi,Yang Hengpan,He Chuanxin
Silver is an extensively investigated electrode material for electrochemical CO 2 reduction owing to its high electrical conductivity and structural stability. However, a key limitation of silver catalysts is their weak adsorption of the *CO intermediate. This intrinsic constraint restricts the reaction primarily to two-electron pathways, hindering the formation of multi-electron products. Here we demonstrate that surface molecular modification can address this limitation. By anchoring bromothymol blue molecules onto the silver surface, we engineer a localized hydrogen-bonding network that stabilizes *CO intermediates via O···H–O interactions, prolonging their surface residence time. In situ spectroscopy and theoretical simulations reveal that this local microenvironment thermodynamically stabilizes *CO against desorption while kinetically facilitating its subsequent hydrogenation and C-C coupling. Consequently, the retained *CO undergoes deeper reduction pathways, generating CH 4 , C 2 H 4 , C 2 H 5 OH, and CH 3 COOH. At a current density of 400 mA cm −2 , the Faradaic efficiency for multi-electron products reaches 24.2%. This work shifts the design paradigm from metal-centric electronic tuning to local microenvironment engineering, offering an alternative strategy for enabling multi-electron transfer on non-copper catalysts.
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EUROPEAN JOURNAL OF MEDICAL RESEARCH
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2026
·Vol. 31
·期 1
·902
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IF 4.8
Ma Yang,Ying Yiyang,Huang Zanche,Zhang Qin,Ren Ning
Background Laser-assisted hatching (LAH) has been reported to improve implantation and pregnancy rates, yet its efficacy and safety profile require rigorous evaluation. To assess the impact of LAH on pregnancy-related outcomes and safety metrics within Assisted Reproductive Technologies (ART) procedures, we conducted a systematic review and meta-analysis. Methods Electronic databases were searched from inception to November 30, 2023. Studies, comparing LAH with other hatching methods or no intervention within ART frameworks, were included. The quality of the included studies was rigorously evaluated using the Risk of Bias In Non-randomized Studies-of Interventions (ROBINS-I) tool with a Weighted Cohen’s kappa coefficient (κ). Summative data, subgroup analyses, and evaluation of heterogeneity were analyzed via Stata 15. Results 47 eligible studies were included, covering 24,876 couples. When comparing the effectiveness of LAH to other methods or no intervention, the results revealed enhancements in implantation and clinical pregnancy rates. For implantation rates, the pooled effect size showed an OR of 1.22 (95% CI 1.09–1.36), indicating a modest improvement with LAH. As for clinical pregnancy rates, LAH was associated with an OR of 1.12 (95% CI 1.03–1.22), demonstrating its positive impact. However, term delivery rates showed a modest but statistically detectable improvement, with an OR of 1.07 (95% CI 1.01–1.14), and sensitivity analysis revealed this effect to be study-dependent. The abortion rate remained statistically unchanged with LAH, OR 0.95 (95% CI 0.85–1.06; P = 0.34; I 2 : 0%). Subgroup analyses, particularly focusing on age and embryo status (fresh vs. frozen), elucidated some of the observed heterogeneity, offering a deeper understanding of LAH's varying impacts across different patient demographics and treatment specifics. Conclusion LAH represents a valuable adjunct in ART, particularly beneficial for specific patient groups. It modestly improves implantation and clinical pregnancy rates without compromising safety. However, its effect on term delivery is small, sensitive to individual studies, and uncertain overall. Graphical
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npj Flexible Electronics
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2026
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IF 15.4
Kan Xinyuan,Fan Qian,Guo Wenlan,Sun Chen,Wang Yan,Duan Xuexin,Xue Qiannan
Ocular blood oxygen saturation is critical for detecting vision-threatening and systemic diseases. Existing wearable oxygen sensors are unsuitable for ocular use, and current ocular SpO₂ measurements rely on bulky equipment. This work introduces a flexible plasmonic nano-confinement (PNC) nanowire photodetector, based on a PNC nanowire array consisting of highly aligned and ordered PEDOT:PSS nanowires with embedded gold nanoparticles, for high-sensitivity oxygen saturation sensing. The PNC nanowire array acts as an anode interfacial modification layer, where nanowire-geometry-induced confinement and localized surface plasmon resonance synergistically lower the hole-extraction barrier, suppress interfacial recombination, and enhance photoconversion efficiency. Its flexible and transparent design, combined with a compact backend circuit using wavelet-threshold denoising, enables integration into a smart contact lens for accurate and stable non-invasive ocular oxygen monitoring. In vivo experiments demonstrate that this smart contact lens could provide a simple, non-invasive platform for continuous ocular SpO₂ monitoring, with potential relevance to systemic oxygen-status assessment.
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Nature Communications
·
2026
·
IF 18.1
Chunyu Suo,Lili Zhang,Qianwei Liang,Chaoyan Chen,Lei Qiu,Ziyi Li,Xinran Zhang,Xuewen Xu,Xiaohua Qi,Xuehao Chen,Jianguo Feng,Xiaodong Yang
Pest infestations devastate crop production, but chemical pesticides pose environmental and health risks. Eco-friendly biopesticides offer a solution, though limited by bioactive compound discovery. Here, we demonstrate that utilization of Yunnan’s Xishuangbanna—a cucumber diversity hotspot—to identify the local variety XSBN032, which retains desirable agronomic traits under aphid pressure. Metabolomic and GC–MS analyses identify farnesal—a volatile compound significantly enriched in XSBN032—under infested and non-infested conditions. Applying 0.33 g/L farnesal nanoemulsion to cucumber leaves rapidly disrupts aphid metabolism (within 6 h), impairing amino acid pathways and suppressing growth/reproduction, achieving 74.9% mortality within 24 h while preserving plant photosynthesis. Significantly, this treatment demonstrates high environmental safety: 0% earthworm mortality (500 mg/L, 7 days) and negligible effects on natural enemies (0% ladybird mortality; 7.41% green lacewing mortality). Our study establishes an integrated pipeline—from resistant germplasm discovery to biopesticide development—providing a sustainable pest control strategy. Leveraging multi-omics screening and smart delivery systems holds promise for future eco-efficient biopesticides. Here the authors show that a sustainable biopesticide can be developed from chemicals produced by a wild cucumber variety. The farnesal nanoemulsion kills 75% of aphids in 24 h while posing minimal risk to the environment and beneficial insects.
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FIBERS AND POLYMERS
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2026
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IF 3.1
Han Jiawei,Wang Yi,Deng Kehui
Electrospun protein–polysaccharide nanofibers are promising biocompatible substrates for skin-contact materials, yet controlling their morphology and hydration behavior in ternary systems remains challenging. In this work, the modification of a cod collagen peptide/poly(ethylene oxide) (CCP/PEO) system with sodium alginate (SA) was investigated as a model polyelectrolyte interaction. A three-factor Box–Behnken design was applied to optimize the spinning-solution preparation for the liquid absorption capacity (LAC), yielding an optimum at a 12.5 h reaction time, 30 °C, and a 30:1 CCP/SA mass ratio. SEM, FTIR, and XRD analyses indicated that electrostatic association and hydrogen bonding between the protonated amino groups of CCP and carboxylate groups of SA facilitated the formation of uniform nanofibers (458 ± 114 nm) with reduced crystallinity. These interactions significantly enhanced the mechanical performance ( p < 0.01), with a 13.1% increase in tensile strength and a 27.3% increase in elongation at break. Compared to unmodified controls, the SA-modified composite mats exhibited a ~ 27% higher LAC (1063.9%) and improved water retention after simulated skin contact, despite a moderate reduction in permeability. A three-stage hydration mechanism—comprising capillary substrate uptake, osmotic swelling within the proposed polyelectrolyte network, and nanofiber dissolution—is proposed to explain the enhanced hydration capacity. This study provides a quantitative process map for the design of high-performance dry-state carriers for functional facial masks.
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JOURNAL OF SOLUTION CHEMISTRY
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2026
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IF 1.3
Chen Yuhuan,Chen Jing,Chen Chunhong,Yang Fang
In this work, n EB-PEI 1800 ( n = 0.22, 0.29, 0.35), a property tunable polymer for CO 2 capture, is reported by partially modifying poly (ethylene imine) (PEI 1800 ) with 1,2-epoxybutane (EB). The density and viscosity of CO 2 -free and loaded aqueous n EB-PEI 1800 solution are measured at the temperatures of (293.15 to 333.15) K and 0.1 MPa. The aqueous solutions with various weight fractions of w = (5, 10, 15, 20 and 30%) are investigated. According to the experimental data, thermal expansion coefficient ( α p ), viscosity activation energy ( E η ) and CO 2 diffusion coefficient ( D CO2 ), are calculated. The influence of temperature, n EB-PEI concentration, CO 2 loading and modification degree are discussed from the viewpoints of molecular interaction and steric effect. The results reveal that low temperature, high n EB-PEI concentration and high CO 2 loading lead to high density and viscosity. Modification degrees have a great impact on the physical properties of n EB-PEI 1800 + H 2 O systems and can reduce the density and viscosity. The density and viscosity values exhibit the decreasing sequence of ρ PEI + H2O > ρ 0.29EB-PEI + H2O > ρ 0.35EB-PEI + H2O ≈ ρ 0.22EB-PEI + H2O and η PEI + H2O > η 0.22EB-PEI + H2O > η 0.35EB-PEI + H2O > η 0.29EB-PEI + H2O , respectively. All the results are attributed to the combined effects of hydrogen bonding, dispersion force and steric structure.
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