Journal of Functional Foods
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2025
·
IF 4
Ping Jiang,Yanfei He,Guangpei Xu,Chuanbo Sun
This study aims to evaluate the mechanism of RCH against CCl 4 -induced liver injury in mice. RCH treatment effectively inhibited CCl 4 -induced liver injury and apoptosis in mice, and reduced liver enzyme activities, blood inflammatory cell and serum inflammatory factor levels. Network pharmacology and machine learning predicted that PI3K-Akt pathway and Met proteins play important roles in RCH anti-CCl 4 -induced liver injury. The results of qRT-PCR and Western blot showed that RCH could down-regulate the expression of the receptor proteins Met, Tlr2, and Tlr4, and inhibit the phosphorylation of Pik3ca and Akt1, and prevent the phosphorylation of NF-κB P65, and decrease cleaved Caspase3 expression. These findings suggest that RCH effectively alleviated liver injury in CCl 4 -induced mice by inhibiting the Met/Tlr2/Tlr4-mediated PI3K-Akt signaling pathway.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2014
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IF 2.829
Feng Chen,Hai-Long Li,Yin-Feng Tan,Yong-Hui Li,Wei-Yong Lai,Wei-Wei Guan,Jun-Qing Zhang,Yuan-Sheng Zhao,Zhen-Miao Qin
Alpinia oxyphylla (Yizhi) capsularfruits are commonly used in traditional medicine. Pharmacological studies have demonstrated that A. oxyphylla capsularfruits have some beneficial roles. Besides volatile oil, sesquiterpenes, diarylheptanoids and flavonoids are main bioactive constituents occurring in the Yizhi capsularfruits. The representative constituents include tectochrysin, izalpinin, chrysin, apigenin-4′,7-dimethylether, kaempferide, yakuchinone A, yakuchinone B, oxyphyllacinol and nootkatone. Their content levels in the fruit and its pharmaceutical preparations have been reported by our group. The nine phytochemicals are also the major components present in the Yizhi alcoholic extracts, which have anti-diarrheal activities. However, the fates of these constituents in the body after oral or intravenous administration remain largely unknown. In the present study, we focus on these phytochemicals albeit other concomitant compounds. The chemicals and their metabolites in rat plasma were identified using liquid chromatography/tandem mass spectrometry with selected reaction monitoring mode after orally administered Yizhi extract to rats. Rat plasma samples were treated by methanol precipitation, acidic or enzymatic hydrolysis. This target analysis study revealed that: (1) low or trace plasma levels of parent chemicals were measured after p.o. administration of Yizhi extract, Suoquan capsules and pills to rats; (2) flavonoids and diarylheptanoids formed mainly monoglucuronide metabolites; however, diglucuronide metabolites for chrysin, izalpinin and kaempferide were also detected; (3) metabolic reduction of Yizhi diarylheptanoids occurred in rats. Yakuchinone B was reduced to yakuchinone A and then to oxyphyllacinol in a stepwise manner and subsequently glucuronidated by UDP-glucuronosyl transferase. Further research is needed to characterize the UDP-glucuronosyl transferase and reductase involved in the biotransformation of Yizhi chemicals.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2015
·Vol. 407
·期 30
·8959-8970
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IF 3.436
Zhao Fengchun,Hu Chunyan,Wang Huimin,Zhao Longyu,Yang Zhengyou
To develop a broad-specificity immunoassay for organophosphorus pesticides (OPs), a broad-specificity monoclonal antibody (MAb) for OPs against a generic hapten, O , O -diethyl O -(3-carboxyphenyl) phosphorothioate with the carboxy group in the meta position of the benzene ring, was produced. Eight haptens were prepared and covalently attached to ovalbumin (OVA) for use as coating antigens, and the optimum coating antigen was selected. Then, a sensitive and broadly class selective competitive indirect enzyme-linked immunosorbent assay (ciELISA) based on the MAb and the optimum coating antigen (hapten H-OVA, possessing an O , O -dimethyl generic structure and linked through a linear spacer arm) was developed and optimized. The MAb developed in this study showed quite different cross-reactivity and selectivity compared to previously produced anti-OPs broad-specificity MAbs. Specifically, the MAb showed high and uniform sensitivity to seven O , O -diethyl OPs and six O , O -dimethyl OPs. With the optimum ciELISA, the IC 50 values of the 13 OPs were determined as 23.1∼151.2 ng mL −1 . The average IC 50 and coefficient of variation (CV) for the IC 50 values of the 13 OPs were 74.6 ng mL −1 and 33.9 %, respectively. For the recovery study, a QuEChERS approach based on dispersive solid-phase extraction (d-SPE) was implemented to decrease the matrix effects of vegetable and fruit samples. The recoveries of six representative OPs from the spiked samples ranged from 89.4 to 135.5 %; the CV ranged from 3.5 to 15.7 %. The ciELISA was also applied to real samples, followed by confirmation with gas chromatography–tandem mass spectrometry (GC–MS/MS) analysis. The results demonstrated that the ciELISA is suitable for monitoring OP contamination in vegetable and fruit samples.
Development of a MAb-based immunoassay for determination of organophosphorus pesticides pretreated with QuEChERS
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高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2025
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IF 3.2
Luo Yuwei,Zhang Yonghua,Chen Xiumei,Che Jinxin,Tao Nengguo
AimsTo overcome the hydrophobicity and effumability restriction of the essential oil in practice, we aim to fabricate the (E)-2-octenal-β-cyclodextrin inclusion complex (O/β-CDIC) and investigate its encapsulation mechanism.Methods and ResultsThe O/β-CDIC was fabricated using saturated aqueous solution method combined with freeze-dried method. The encapsulation efficiency and loading content of O/β-CDIC were 45.61 ± 1.37% and 2.97 ± 0.08%. In vitro assays, the MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of the O/β-CDIC were 2.00 and 8.00 mg·mL⁻¹ for P. italicum XX5 mycelial growth, respectively. In addition, the O/β-CDIC effectively reduced the incidence of blue mold in citrus fruit and maintained fruit quality. The detailed structure characterization combined with the molecular docking study, molecular electrostatic potential, and independent gradient model based on Hirshfeld analysis collectively demonstrated that (E)-2-octenal was entrapped successfully within the β-CD cavity.ConclusionsThe O/β-CDIC significantly enhanced the thermal stability and phase solubility of (E)-2-octenal while effectively preserving its antifungal activity. Characterization analyses confirmed the presence of non-covalent interactions between β-CD and (E)-2-octenal.
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Ziyang Bian,Lili Huang,Zetong Li,Jie Wang,Yue Wang,Zhenshan Yang,Hefu Li
The development of versatile surface-enhanced Raman scattering (SERS) platforms facilitates the rapid identification of contaminants in diverse application scenarios. Herein, a dual-functional SERS substrate comprising silver nanoislands-decorated micropillar/wrinkle hierarchical structure (Ag NIs@PWHS) on a polydimethylsiloxane (PDMS) film was fabricated through an amalgamation of fabrication techniques: soft lithography, surface wrinkling, and magnetron sputtering. The PDMS hierarchical architecture was constructed by replicating mastoid structures of lotus leaves through a two-step molding process, followed by the formation of periodic wrinkles via oxygen plasma treatment. This hierarchical design integrating micropillars and wrinkles not only provided a multiscale surface architecture conducive to the formation of three-dimensional (3D) hot spots, but also conferred hydrophobicity and bending insensitivity on the SERS substrate. The optimal Ag NIs@PWHS substrate had an analytical enhancement factor of 1.99 × 10 6 , outstanding signal uniformity (relative standard deviation, RSD = 2.98 %) and reproducibility (RSD = 4.54 %), and robust mechanical stability. Furthermore, the Ag NIs@PWHS substrate enabled the rapid detection of thiram on pear surface and malachite green in lake water, with a detection sensitivity of 10 −7 M for both pesticides. Thus, the developed Ag NIs@PWHS SERS substrate holds significant potential for utilization in realms of food safety assessments and water pollution surveillance.
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Food Bioscience
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2025
·
IF 5.9
Zi-Xuan Ding,Yue-Hua Chen,Jia-Nuo Zhang,Wen-Yu Wang,Xin-Ru Zhang,Rui-Bo Sun,Hui Zhang,Ming Xie,Hui-Peng Song
Processed Buddha's hand fruit (PBHF) is a widely consumed food, but its flavor profile and functional mechanisms remain understudied. In this study, human sensory evaluation (n = 120), E-tongue, E-nose, microbiomics, and metabolomics were integrated to comprehensively analyze PBHF's flavor characteristics and elucidate its dietary improvement mechanisms. Human sensory evaluation identified PBHF as predominantly sweet and sour with a soft texture, accompanied by subtle notes of saltiness, pomelo peel, liquorice, refreshment, hawthorn, fragrant aroma, and cinnamon. These findings were corroborated by E-tongue analysis, which confirmed sweetness and sourness as the primary tastes. E-nose detection revealed 36 aroma components, including limonene as the key contributor to the pomelo peel flavor. In animal studies, PBHF regulated dietary patterns in mice, significantly reducing weight gain and enhancing gastrointestinal motility. Importantly, it showed no adverse effects on organ indices (spleen, kidney, liver, or thymus). Microbiomics analysis indicated that PBHF increased the abundance of digestive bacteria such as Limosilactobacillus reuteri , Lacticaseibacillus johnsonii , and Ligilactobacillus murinus . Metabolomics profiling further demonstrated that PBHF mitigated weight gain by upregulating metabolic pathways such as lipolysis in adipocytes, fatty acid degradation, and glycerolipid metabolism. By synergizing large-scale human sensory evaluation, electronic sensory evaluation, microbiomics, and metabolomics, this study systematically deciphered PBHF's flavor attributes and mechanistic roles in promoting digestion and metabolic health. These findings provide robust scientific evidence for PBHF's potential applications in functional food development.
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高影响因子
NEW JOURNAL OF CHEMISTRY
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2025
·Vol. 49
·期 27
·11985-11995
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IF 2.5
Yu Chen,Ziteng Yang,Yanlong Wang,Guojian Zhao,Taoran Wang,Minghui Feng,Mengxi Zhao,Xihou Wang,Dexin Dong
Lithium iron phosphate (LFP)-based lithium-ion batteries (LIBs) are widely used in various fields due to their advantages such as low cost, long service life, and high energy density. The widespread use of LFP-based LIBs leads to the generation of large quantities of spent LFP cathode materials, which contribute to environmental pollution and resource waste. Therefore, it is necessary to develop a new strategy to recycle spent LFP cathode materials from LIBs. Here, we use non-toxic, low-cost and naturally available vegetable-/fruit-derived solvents (VFSs) to efficiently and mildly recover LFP cathodes from LIBs. The highest leaching efficiency of Li and Fe from LFP using VFSs is 98.8% and 99.8%, respectively. The superhigh leaching efficiency of Li and Fe achieved by VFSs is comparable to that obtained using other green solvents, including ionic liquids, deep eutectic solvents, and low-melting mixture solvents; however, VFSs in this work are greener, cheaper and even could be edible. In addition, a high precipitation efficiency of Li (83.4%) and Fe (98.5%) could be achieved at room temperature after screening 69 kinds of antisolvents.
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Science China-Chemistry
·
2013
·Vol. 56
·期 11
·1641-1650
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IF 1.327
Qing XiangDong,Wu HaiLong,Nie ChongChong,Li Yong,Yan XiuFang,Zhang XiHua,Yin XiaoLi,Yu RuQin
This work presents a novel application of second-order calibration based on self-weighted alternating trilinear decomposition (SWATLD) algorithm for analyzing the HPLC-DAD data. The proposed method makes it possible to simultaneously determine teflubenzuron, hexaflumuron, flufenoxuron, chlorfluazuron, diflubenzuron and benzoylurea in different fruit samples, i.e. pear, apple and banana, in the selected time region of chromatogram. The concentration, elution time and spectral information of these benzoylurea insecticides are selectively extracted from complex matrices even in the presence of unknown interferences. The root-mean-square error of prediction (RMSEP) and figures of merit, including sensitivity (SEN), selectivity (SEL) and limit of detection (LOD) are employed to access the performance of the method. The LODs obtained for these insecticides are within the range 0.017–0.26 ppm in pears, 0.039–0.33 ppm in apples, 0.041–0.44 ppm in bananas, respectively. Such a chemometrics-based protocol holds great potential to be extended as a promising alternative for more practical applications in food safety and quality monitoring.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
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IF 5.6
Tao Li,Yongtao Yan,Linlin Han,Mingkun Li,Shiyu Liu,XinYang Zhou,Jungjoon Lee,Xiaomin Li,Yuqing Zhao
Rare saponins show numerous significant biological activities , however, there has been no research on using ginseng fruit rare saponins (GFRS) to minimize skin wrinkles. This paper studied the anti-wrinkle effect, related mechanism, material basis of efficacy, and structure-activity relationship of GFRS in vivo and in vitro . The findings demonstrate that GFRS has potent collagenase and elastase inhibition capabilities in addition to superior ultraviolet absorption properties. Secondly, GFRS can strengthen the endogenous antioxidant defense system, thus lessening the UVB-induced ultraviolet damage that L929 cells and zebrafish experience. Matrix metalloproteinases (MMPs) studies have shown that GFRS could significantly inhibit the UVB-induced MMP1 and MMP9 proteins production. Finally, the main ginsenoside component in GFRS was identified by LC-MS, and 16 kinds of ginsenoside were analyzed, including rare ginsenoside such as Rg 6 , F4, and R/S-Rg 3 , which may be caused by the transformation of prototype saponins Re and Rd caused by citric acid heat treatment (CAHT). After doing a quantitative HPLC analysis on twelve of them, it was discovered that CAHT could enrich the content of rare saponins in GFS. By evaluating these ginsenoside’s anti-wrinkle properties. It is found that the main material basis for GFRS’s anti-wrinkle action may be ginsenoside Rg 6 or F4. In summary, GFRS exhibits promising properties as an anti-wrinkle treatment because of its robust anti-oxidation, anti-collagenase, anti-elastase activity, favorable ultraviolet absorption effect, and capacity to regulate MMPs in vivo and in vitro models.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiayue Jin,Zihan Cai,Weinan Huang,Jiahao Zhong,Di Wu,Chen Chen,Bangdi Liu,Yanna Shi,Xian Li,Kunsong Chen
Loquat fruit is vulnerable to postharvest chilling lignification. Fumigation with methyl jasmonate (MeJA) could inhibit the lignification, but is inefficient. The present study developed nanoparticles encapsulated with MeJA (MeJA@NPs) with high encapsulation efficiency (94.83 %) and 30 d sustained release capability. Then, a transparent, edible, in situ coating was prepared by blending a gelatin/pullulan solution with MeJA@NPs. The coating could continuously inhibit chilling lignification of loquat fruit, where the firmness increase and lignin accumulation were reduced by 82.7 % and 52.3 % respectively after 6 d of storage. Further analysis of the inhibition mechanism showed that the coating suppressed the peroxidase activity, thereby blocking lignin polymerization. Besides, the coating could also enhance the antioxidant capacity of fruits via activating cinnamate 4-hydroxylase and 4-coumarate: CoA ligase and upregulating the superoxide dismutase, catalase, and ascorbate peroxidase activities. In conclusion, our work provides a novel approach of in situ nanoparticle coating on fruits for postharvest preservation.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Hui-Wen Wang,Jia-Qi Wang,Yue-Qiu,Tong-Hao Cui,Jian-Cong Liao,Yi-Long Liu,Ya-Qin Liu,Yuan-Jiang Pan,Xian Li,Xiao-Yong Zhao
Universal 5-Da labeling creates internal standards for accurate N-glycome analysis. • Novel matrix 2-AIA/CHCA ensures uniform co-crystallization and high reproducibility. • H3N2F1X1 is a cross-species candidate biomarker for fruit cold stress. N-glycosylation is a crucial post-translational modification regulating protein function in plants. However, its accurate quantification by matrix-assisted laser desorption/ionization-mass spectrometry with time-of-flight is challenged by low glycoprotein abundance, poor glycan ionization, uneven matrix crystallization, and insufficient internal standards. Here, we introduce a novel 5-Da stable isotope labeling method, combining enzymatic 18 O-labeling (+2-Da) and reduction with NaBD 4 (+3-Da) on a portion of the sample itself to universally generate internal standards that eliminate isobaric interference. Furthermore, a binary matrix of 2-aminoisophthalic acid (2-AIA) plus α-cyano-4-hydroxycinnamic acid (CHCA) was developed, providing high sensitivity and homogeneous co-crystals. The integration of isotope labeling with 2-AIA/CHCA allows linear quantification ( R 2 > 0.99) over a two orders of magnitude. Applied to tomato and peach during cold storage, the method revealed that H3N2F1X1, the most abundant N-glycan in both fruits, was significantly downregulated, suggesting its role as a candidate biomarker for cold stress response and fruit quality control. Download: Download high-res image (204KB) Download: Download full-size image
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Lianfeng Luo,Jieqiong Wang,Meiqin Li,Yingbin Zhang,Yuwan Wang,Yongquan Xu,Haisheng Chen,Yan Zhu,Zhihui Feng,Junfeng Yin
With a distinctive aroma, Shanhuangpi (SHP), the fruit of the plant Clausena anisum-olens (Blanco) Merr., has been newly developed as a commercial fruit resource. The fresh fruit deteriorates rapidly due to its high respiration and metabolism. Therefore, an effective drying process is urgently needed for its preservation. The influence of two commonly used drying methods for plant materials, i.e., vacuum freeze-drying (VFD) and hot air drying (HAD) was evaluated on the aroma, taste, color, and volatile profiles of this fruit. The results indicated that the VFD process well retained the color and volatile composition of the fresh fruit but produced extra graininess and sticky mouthfeel. Alternatively, the HAD process offered a shorter drying time, kept a more palatable sour/sweet ratio, but created a darker brown color and shrinking texture of the fruit. The volatile components were enriched by the micro-chamber/thermal extractor (μ-CTE) and 38 compounds were identified with gas chromatography-mass spectrometry. Sixteen odor-active compounds (detection frequency ≥4) were detected by gas chromatography–olfactometry. Results obtained in this study can be served as a reference for the evaluation and further development of SHP.
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高影响因子
危险品化学品经营许可证（带存储）