BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2024
·
IF 2.5
Jianyu Xia,Pengju Guo,Jing Yang,Tao Zhang,Kejian Pan,He Wei
Background Colorectal cancer (CRC) is a prevalent malignancy and poses a significant clinical challenge. Piperine, an alkaloid molecule extracted from Piper nigrum and Piper longum, has emerged as a promising anticancer agent. However, the molecular mechanisms of piperine’ antitumor effects in CRC need to be further elucidated. Methods Human colorectal cancer cells were treated with piperine in vitro . CCK-8 and clone formation assays were adopted to detect cell viability. The accumulation of autophagosomes was assessed by Western blotting and immunofluorescence. Apoptosis and reactive oxygen species (ROS) levels were analyzed by flow. In vivo , a xenograft tumor mouse model was constructed using CT26 cells. Results Piperine inhibited CRC cell viability and suppressed tumor weight and volume in a mouse model. Additionally, piperine treatment induced the accumulation of autophagosomes in CRC cells. This effect was attributed to the inhibition of the AKT/mTOR pathway and the accumulation of ROS. activation of AKT or clearance of ROS attenuated piperine-mediated tumor suppression. Conclusion This study shows that piperine induces autophagy-dependent cell death in CRC cells by increasing ROS production and inhibiting Akt/mTOR signaling.
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Qianqian Zhao,Min Gu,Mengnan Ni,Jinyu Li,Ting Wu,Senlin Zhu,Yupeng Zhou,Yawen Lu,Xiaolin Li,Huae Xu,Meiping Lu
Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa mediated by immunoglobulin E (IgE) after exposure to allergens. The bothersome symptoms of AR, such as runny nose and nasal congestion, affect millions of people worldwide. Ipratropium Bromide (IB), commonly used in clinical practice for treating AR, requires frequent administration through nasal spray and may cause significant irritation to the nasal mucosa. The induction of ROS is closely related to the initiation and symptoms of AR, and ROS will continue to accumulate during the onset of AR. To address these challenges, we have designed a drug delivery system that can be administered in liquid form and rapidly crosslink into a ROS-responsive gel in the nasal cavity. This system enables sustained ROS responsive release of IB in a high-concentration ROS environment at AR lesions, thereby alleviating AR symptoms. The gel demonstrated prolonged release of IB for up to 24 hours in rats. In the treatment of AR rat models, it improved their symptoms, reduced the expression of various inflammatory factors, suppressed MUC5AC protein expression, and decreased mucus secretion through a ROS responsive IB release pattern. Overall, this system holds promise as a better option for AR treatment and may inspire the design of nanogel-based nasal drug delivery systems.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2021
·Vol. 168
·期 1
·016504
·
IF 4.316
Haomin Jiang,Yujuan Cheng,Zhaohui Wang,Zhiqun Bai,Yang Tang,Yanzhi Sun,Pingyu Wan,Yongmei Chen
The cleavage of the linkage bonds among the C9 units in lignin molecules via selective oxidation method to obtain small-weight aromatic compounds is an important way to lignin valorisation. In this study, the cleavage pathways of bonds in a lignin model compound, GGE (guaiacylglycerol-β-guaiacyl ether), by the reactive oxygen species (ROS) in situ generated through electrochemical oxygen reduction reaction (ORR) in an aprotic ionic liquid ([BMIM]BF4) was investigated. The results indicated that when the main ROS was ·OOH, coniferyl alcohol, guaiacol and vanillin were detected as the degradation products which is supposed to be caused by β-O-4 bonds cleavage; Otherwise, when HO2− was the main ROS, coniferyl alcohol could not be detected and the degradation reaction is believed to be trigerred by Cα-Cβ bonds cleavage.
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
Qingmei Zhang,Yuanming Sun,Kaitao Yao,Siyuan Cheng,Yifei Zhou,Haoyu Wang,Fei Lei,Yiran Yin,Yingfu Jiao,Weifeng Yu,Liqun Yang,Yuanhai Li,Po Gao,Xingtao Chen
A Ce 3+ -crosslinked injectable self-healing hydrogel with pH responsiveness is developed for wound analgesia and ROS removal. • Hydrogel enables rapid drug and cerium release in acidic wounds, while achieving sustained release with minimal leakage at physiological pH. • Acid-triggered cerium release achieves ≥78% ROS clearance, with the hydrogel maintaining excellent cytocompatibility (viability ≥95%) at neutral pH. • A single perilesional injection achieves 36-hour analgesia in the mouse plantar incision model, significantly surpassing free ropivacaine. • Analgesia is achieved by inhibiting TRPV1+ neuron activation in DRG and spinal dorsal horn, with reliable biosafety and intact wound healing. Since tissue acidification in wounds is tightly linked to pain and inflammation, pH-responsive hydrogels are promising candidates for local analgesia and ROS scavenging in wound management. Herein, an injectable, self-healing and pH-responsive hydrogel crosslinked by coordination bonds between cerium ions and carboxylate moieties of carboxymethyl cellulose (CMC) is developed to encapsulate ropivacaine (Rop). The hydrogel features rapid matrix dissolution accompanied by fast release of both Rop and cerium ions under acidic conditions (pH 6.2), while providing sustained Rop release and extremely limited cerium ion release at physiological pH of 7.4. These features contribute to prominent antioxidant activity (ROS clearance rate ≥78%) under acidic conditions, and excellent cytocompatibility (cell viability ≥95%) at pH 7.4. Injecting the hydrogels into mouse plantar incisions resulted in prolonged analgesia (36 h) matching their Rop release feature, alongside excellent biosafety without impairing wound repair. Immunofluorescent assays demonstrate that such analgesic effects are realized by inhibiting the activation of TRPV1 + neurons in the DRG and neurons in the spinal dorsal horn. These findings provide an injectable, self-healing and pH-responsive hydrogel crosslinked by cerium ion coordination for local anesthetic loading and antioxidation in wound healing. Download: Download high-res image (104KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Rongjian Ding,Xiangchuan Xu,Haoyang Ding,Zhen Chen,Hao Xia,Shujun Cao,Zhongwei Gu,Hongli Mao
Injectable hydrogels have great potential in wound repair applications due to their simple operation and ability to adapt to irregular wounds. However, the current injectable hydrogels have poor biocompatibility, insufficient hemostatic performance, and low wound healing efficiency, which limit their application in wound repair. In this study, an injectable hydrogel (TS@EP) was constructed through a catalyst-free amide reaction between NHS ester-terminated four-arm poly(ethylene glycol) (4aPEG-SS), gallic acid-modified ε-poly(l-lysine) (ε-PLL-GA), and amino-terminated Pluronic F127 (APF). The prepared hydrogels have excellent mechanical properties, strong tissue adhesion, and excellent biocompatibility. In addition, the TS@EP hydrogel exhibited remarkable antibacterial and antioxidant capacities, which are critical for preventing bacterial infection and scavenging excessive reactive oxygen species (ROS) in wound sites. The results of animal experiments have confirmed that the TS@EP hydrogel can effectively seal the wound, thereby achieving rapid hemostasis and also having the function of promoting wound healing. In conclusion, the TS20@E2.5P7.5 hydrogels have great promise as a biomaterial for wound hemostasis and seamless closure, offering a potential alternative to conventional wound management strategies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Ziyi Lin,Peng Xu,Yuehai Xu,Yixin Zheng,Huimin Li,Zixin Chen,Zhe Wang,Shaochen Song,Yuhao Liu,Zhao Yang,Ju Cui,Heyun Shen
The therapeutic potential of nanomaterials in metabolic dysfunction-associated steatohepatitis (MASH) management remains limited by suboptimal drug loading efficiency and insufficient modulation of the disease’s multifactorial pathogenesis. To address these issues, we developed a fully active pharmaceutical ingredient (API)-based nanodrug designed for synergistic MASH therapy through three integrated mechanisms: dual modulation of reactive oxygen species (ROS) homeostasis via endogenous and exogenous pathways, mitigation of pathological lipid accumulation, and suppression of inflammatory cascades. The nanodrug (QRDP) system utilizes arginine-modified polydopamine as both a nanocarrier and intrinsic antioxidant and codelivery of quercetin and the mitochondrial uncoupler 2,4-dinitrophenol. QRDP eliminates more than 80% of ROS and increases hepatic glutathione levels by 1.5-fold through exogenous antioxidant delivery and endogenous ROS suppression via regulating mitochondrial proton channels. Additionally, QRDP-mediated mitochondrial uncoupling reduced intracellular lipid overload by 90% through inhibition of ATP synthesis, accompanied by significant decreases in serum total cholesterol (39%), triglycerides (30%), and free fatty acids (59%). Furthermore, QRDP significantly downregulated pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) in vitro and in vivo, while restoring anti-inflammatory markers (Arg-1 and IL-10) to over 78% of baseline levels. In high-fat diet (HFD)-induced MASH mice, QRDP treatment not only attenuated hepatic fibrosis and normalized liver function but also provided substantial protection against lipotoxicity. This full-API nanotherapeutic platform represents a safe and effective multitarget strategy for comprehensive MASH intervention.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-20
·
IF 12.6
Shen Jiayi,Luo Yuwen,Hu Wuming,Zhang Susu,Yang Yu,Lan Linwen,Liu Enqian,Xu Jian,Hu Tingting,Nie Jingjun,Chen Dafu,Liang Ruizheng,Lyu Lingchun
Myocardial ischemia/reperfusion (I/R) injury represents a major global health concern, driven by excessive reactive oxygen species (ROS), which closely correlates with subsequent myocardial infarction and fibrosis. Combining ROS scavenging with ischemic myocardium revascularization holds therapeutic promise. However, developing multifunctional therapeutic agents that can simultaneously scavenge ROS and promote angiogenesis remains unreported. Herein, we propose MgSrCeAl-layered double hydroxide (LDH) nanosheets as a multifunctional ROS scavenger and angiogenesis promoter for efficient myocardial I/R injury treatment. MgSrCeAl-LDH nanosheets not only exhibit exceptional hydroxyl radicals (·OH) and superoxide anions (·O 2 − ) scavenging capabilities, which is 1.78 times and 1.61 times that of CeO 2 , respectively, but also respond to the ischemia-induced acidic microenvironment to release angiogenic Sr 2+ with a concentration of 32.65 ppm (pH 5.0), which is conducive to efficient angiogenesis. Specifically, the ROS levels in I/R-injured myocardium decreased to 66.2% of its original value following intervention with MgSrCeAl-LDH nanosheets, while the vascular density in the peri-ischemic myocardium increased by approximately 3.6-fold. Ultimately, the infarcted myocardial area is reduced 60.9% compared to the group without MgSrCeAl-LDH intervention. Mechanistically, MgSrCeAl-LDH nanosheets alleviate myocardial infarction and fibrosis caused by I/R injury through activating PI3K/Akt pathway and inhibiting TGF-β pathway, ultimately improving cardiac function and inhibiting ventricular remodeling. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Haixing Feng,Jionghong Liang,Aiyi Chen,Xutong Zhou,Haojie Liu,Zhaoxi Zhao,Ming Wu,Jiangwen Liu,Guie Xie
Metallic implants in diabetic patients face a critical dual challenge: excessive reactive oxygen species (ROS) and advanced glycation end-products (AGEs) accumulation severely impair osteointegration, while suppressing ROS is beneficial for tissue repair but compromises antibacterial defense. Here, we report an innovative multifunctional coating composed of manganese oxide nanoparticles and polydopamine (PDA) on a nano-network metallic substrate (TAMP), which enables dynamic regulation of ROS and near-infrared-triggered antibacterial activation. Under near-infrared irradiation (NIR), the coating induces mild photothermal heating (PTT) and synergistic photodynamic therapy (PDT) effects, leading to moderate ROS generation and achieving over 95% bactericidal efficiency against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) without damaging host tissue. In the absence of light, controlled manganese ion release scavenges excess ROS, restores redox balance, and promotes human mesenchymal stem cell proliferation. The coating exhibits long-term antibacterial stability, maintaining more than 96% bactericidal activity after 7 days, and enhanced corrosion resistance with corrosion current reduced to 9.88% of that of the bare substrate. This dual-mode, light-controllable, and ROS-adaptive system provides an innovative solution to reconcile infection control and tissue regeneration in diabetic oxidative microenvironments, offering a promising strategy for next-generation metallic implants.
✓ 已验证
产品关联
DOI
高影响因子
Materials Advances
·
2026
·
IF 5.8
Lian Ren,Xiaobin Zhou,Longbao Feng,Guodong Sun
Spinal cord injury (SCI) induces the formation of a complex pathological microenvironment characterized by excessive reactive oxygen species (ROS) accumulation, persistent hypoxia and severe inflammatory responses, which severely impairs the processes of neural regeneration and functional recovery. To address these critical issues, we constructed a hydrogel system for the synergistic repair of SCI by integrating magnesium-manganese layered double hydroxides (MgMn-LDHs) into a ROS/pH-responsive GelMA-PBA/HA-DA hydrogel matrix. Harnessing the redox activity of Mn3+, MgMn-LDHs efficiently scavenge excess ROS and create a regenerative microenvironment favorable for neural repair. Meanwhile, Mg2+ promotes axonal elongation, myelination and the polarization of anti-inflammatory M2 macrophages by regulating key neural differentiation signaling pathways. The GelMA-PBA/HA-DA hydrogel, crosslinked via dynamic boronate ester bonds, enables the precise and controlled release of MgMn-LDHs in the acidic and highly oxidative pathological microenvironment post-SCI. In vitro experiments demonstrated that this hydrogel system could effectively support the proliferation and neurite outgrowth of PC12 cells under hypoxic and ROS-enriched conditions, inhibit the secretion of pro-inflammatory cytokines, and significantly promote vascular regeneration. In vivo studies in a mouse SCI model revealed that the hydrogel system markedly improved locomotor function, reduced the expression levels of inflammatory markers, ameliorated the inflammatory status of injured tissues, and effectively facilitated axonal regeneration and remyelination at the injury site. This MgMn-LDHs-loaded GelMA-PBA/HA-DA hydrogel system establishes a multifunctional and translatable therapeutic platform for SCI treatment, and also provides valuable insights for the research on therapeutic strategies against other neurodegenerative diseases.
✓ 已验证
产品关联
DOI
高影响因子
VIEW
·
2026
·
IF 8.1
Guangrong Zheng,Tengfei Ke,Hongjin Wang,Hong Huang,Ying Zhang,Guanghai Fei,Chengde Liao,Haiyan Yang
The antioxidant defense system (ADS) within the tumor microenvironment restricts the efficacy of reactive oxygen species (ROS) based therapies by conferring intrinsic redox tolerance. To overcome this limitation, we develop a tumor cell membrane camouflaged liposomal nanoplatform (C-MLip@LA) that modulates the ADS to enhance tumor-targeted therapy through a synergistic reactive oxygen/nitrogen species (ROS/RNS) cascade. The system encapsulates L-arginine (LA) as the core, anchors MnO 2 nanoparticles on the liposomal surface, and is cloaked with homologous tumor cell membranes, thereby enabling tumor-specific recognition and immune evasion. C-MLip@LA disassembles after cellular internalization, where MnO 2 depletes intracellular glutathione (GSH) and is reduced to Mn 2 + , thereby elevating ROS levels and enabling T 1 -weighted magnetic resonance imaging. Concurrently, the released LA reacts with endogenous H 2 O 2 to generate nitric oxide (NO). The interplay between NO and ROS initiates a cascade reaction that produces highly cytotoxic peroxynitrite, thereby amplifying immunogenic cell death and leading to pronounced tumor growth inhibition in a CT-2A tumor model. Collectively, this work presents a biomimetic nanoplatform for redox-modulated cancer therapy with integrated diagnostic and immunomodulatory functions.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2026
·
IF 4.7
Aolei Hu,Xilu Chen,Haimei Ling,Zhenzi Mi,Ruilian Yu,Yao Fu
Malignant melanoma has a high recurrence rate after surgical resection, conventional neoadjuvant chemotherapy (NAC) suffers from off-target toxicity and limited efficacy. Leveraging elevated tumor-specific reactive oxygen species (ROS), we designed a sequential therapeutic strategy combining ROS-responsive micelles and a multifunctional hydrogel to combat local recurrence. By conjugating 3-indoleacetic acid to chondroitin sulfate via a ROS-responsive thioketal linker, an amphiphile (CS-TK-IAA, CTI) was synthesized, significantly enhancing tumor-specific uptake and ROS-scavenging capacity. CTI self-assembled into micelles (CLT@CTI) encapsulating celastrol (CLT), achieving high encapsulation efficiency (92.3%) and drug loading capacity (25.2%). Postoperatively, an injectable chitosan-based hybrid hydrogel (CPG) with self-healing, tissue adhesion, and hemostatic properties was applied to the resection site, triggering a photothermal effect via near-infrared light. In a mouse melanoma recurrence model, neoadjuvant CLT@CTI therapy effectively suppressed primary tumor growth, while postoperative CPG gel-mediated photothermal ablation of residual tumors dramatically delayed recurrence and improved 60-day survival rates. This sequential therapeutic regimen, neoadjuvant chemotherapy with postoperative photothermal therapy, provides a promising and viable solution for preventing melanoma recurrence.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2026
·
IF 4.4
Mengxia Ji,Zhen Tao,Tiange Wei,Yifan Shao,Qing Xu,Jinman Yang,Jiexiang Xia,Huaming Li
A direct Z -scheme heterojunction was constructed between carbon nitride and BiOBr 0.5 I 0.5 ultrathin nanosheets. • The optimized composite achieved 84% BPA removal efficiency under visible light irradiation within 40 min. • The internal electric field at the heterointerface drives a direct Z-scheme charge transfer pathway. • The h + , •O 2 − , and •OH were identified as dominant active species via ESR and scavenging experiments. • Enhanced spatial carrier separation and preserved redox ability synergistically boost photocatalytic degradation activity. Bismuth oxyhalide-based solid solutions have excellent visible light absorption and oxidation capabilities, but limited charge separation efficiency hinders their applications. In this study, a Z-scheme heterojunction involved ultrathin polymeric carbon nitride (CN) and BiOBr 0.5 I 0.5 solid solution (BOBI) nanosheets was synthesized for BPA photodegradation. The well-matched energy bands and work function difference between CN and BOBI promote efficient separation and transfer of photogenerated carriers. Meanwhile, CN/BOBI retains the strong reduction capacity of CN and the strong oxidation capacity of BOBI, which is conducive to the generation of more reactive oxygen species (ROS, •OH and •O 2 − ) and holes. Based on these advantages, CN/BOBI exhibits excellent bisphenol A (BPA) removal performance. The optimized CN/BOBI-2 composite achieves an 84% BPA removal efficiency under visible light irradiation. This work establishes a scalable strategy for modulating solid solution photocatalysts via heterojunction engineering, which can be applied to sustainable environmental remediation. Download: Download high-res image (213KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）