CHEMICAL PHYSICS LETTERS
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2019
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IF 1.901
Mi Zhang,Yu-He Zhang,Ting-Mei Ma,Han Zeng
Carbon nano-tubes as well as nano-composite consisting of carbon tubes and nano-gold particles after modification of aromatic substituent were proposed as carriers to immobilize myoglobin molecules for preparation of Myoglobin based electrodes. Feature of morphology, surface structure for nano-materials with Myoglobin integration, configuration of redox sites in protein and physic-properties of coupled Myoglobin molecules were characterized and investigated by various technical means. Results from experiments showed that electro-active groups of nano-materials with entrapped Myoglobin demonstrated rapid mass transferring. Interaction between protein and substitutional groups grafted on nano-materials would increase the active energy of electron transferring and transformation of H 2 O 2 .
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BIOTECHNOLOGY LETTERS
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2019
·Vol. 42
·期 2
·269-276
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IF 2.154
Cai Xinyong,Zhang Mengjie,Wei Wei,Zhang Yinjun,Wang Zhao,Zheng Jianyong
A novel enzyme immobilization method employing metal–organic framework (MOF) encapsulation and macroporous resin adsorption was developed in this study. Candida antarctica lipase B (CALB) was firstly encapsulated onto metal–organic frame structures (Zeolitic imidazole framework-8, ZIF-8) and further bonded to macroporous resin by physical adsorption. Under optimized immobilization conditions, the activity of the prepared immobilized lipase (CALB-ZIF-8@D101) determined via the methyl esterification of oleic acid was 38.4 U/mg. Compared with free lipase, the immobilized lipase exhibited improved thermal and operational stability and organic solvent tolerance. These results demonstrate that the immobilization method of ZIF-8 encapsulation and macroporous resin adsorption enhanced enzyme properties at a superior level.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2026
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IF 1.7
Wang Xinyu,Qin Yilin,Gong Junyuan,Zhang Guohao,Peng Yinyin,Sun Gen,Ji Yanhao,Huang Yaling,Li Yahui,Nie Xiaoqin
This study aimed to enhance U(VI) removal from water by constructing a ternary system of Shewanella putrefaciens ( S. putrefaciens ), chlorite, and humic acid (HA). The ternary system achieved significantly higher U(VI) immobilization than single or binary systems, owing to synergistic effects of adsorption enrichment, electron shuttling, and bioreduction. Under optimal conditions, the U(VI) removal efficiency reached 91.6%. Importantly, pre-forming the ternary composite before U(VI) addition avoided false removal caused by HA-U(VI) complexation, ensuring accurate assessment of reductive immobilization. This work provides a reliable strategy for uranium-contaminated water remediation. Graphical abstract
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
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IF 9.9
Yangjie Li,Yirong Tian,Yu Fu,Liang Pang,Yang Li,Peng Xiao,Zhuan Li
Smart surface coatings have been proven to be an effective strategy to significantly enhance the electronic conductivity and cycling stability of silicon-based anode materials. However, the single/conventional coatings face critical challenges, including low initial Coulomb efficiency (ICE), poor cyclability, and kinetics failure, etc. Hence, we proposed a dual immobilization strategy to synthesize graphene supported anatase TiO 2 /carbon-coated porous silicon composite (denoted as PSi@TiO 2 @C/Graphene) using industrial-grade ferrosilicon as lithium storage raw materials through the simple etching, combined with sol–gel and hydrothermal coating processes. In this work, the dual immobilization from the “confinement effect” of the inner TiO 2 shell and the “synergistic effect” of the outer carbon shell, improves the kinetics of the electrochemical reaction and ensures the integrity of the electrode material structure during lithiation. Furthermore, the introduction of the graphene substrate offers ample space for dispersing and anchoring the Si-based granules, which in turn provides a stable 3D conductive network between the particles. As a result, the PSi@TiO 2 @C/Graphene electrode delivers high reversible capacity of 1605.4 mAh g −1 with 93.65% retention at 0.5 A g −1 after 100 cycles (vs. 4th discharge), high initial Coulomb efficiency (82.30%), and superior cyclability of 1159.9 mAh g −1 after 250 cycles. The above results suggest that the particle structure has great potential for applications in Si-based anode and may provide some inspiration for the design of other energy storage materials.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
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IF 5.268
Yao Zhang,Ling Zhu,Gangcheng Wu,Xingguo Wang,Qingzhe Jin,Xiguang Qi,Hui Zhang
In this study, hollow mesoporous silica cube (HMSC) modified with amino (-NH 2 ) were synthesized and applied in the immobilization of phospholipase D (PLD) via physical adsorption and chemical cross-linking strategy. The amino-functionalized nano carrier HMSC represented excellent immobilization ability and achieved 87.15 % immobilization rate. The immobilized PLD has wider pH application range and thermal stability, and maintained over 90% of the initial activity after incubation at 50 °C for 2 h. After 50 days of storage at 4 ℃, immobilized PLD retained 40.12 % of its initial activity while free PLD lost 88.28% of its initial activity. The modified HMSC with immobilized PLD (HMSC-NH 2 -PLD) retained 50.73% activities after 9 consecutive reuses. Using the HMSC-NH 2 -PLD, a high-efficient method for the conversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and L-serine was proposed. The HMSC-NH 2 -PLD exhibited prominent enzymatic activity for PS synthesis, the maximal conversion of PS was 90.40% with a catalytic efficiency (CE) of 31.95 μmol / (g h under the optimal conditions. The research in this paper provides a sustainable and efficient biocatalysis application for PS synthesis.
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Environmental Technology & Innovation
·
2021
·
IF 5.263
Yansong Zhang,Zehai Yu,Yunshuang Hu,Caifeng Song,Fuwei Li,Wen He,Xiaofeng Wang,Zhixia Li,Hongfei Lin
Magnetic polyvinyl alcohol–sodium alginate–diatomite (PVA–SA-diatomite) composite carriers for immobilized microorganisms were prepared for removal of NH 4 + -N from effluents. The mass transfer efficiency, chemical stability, and NH 4 + -N removal efficiency of the carriers were investigated. The amount of each ingredient added significantly affected the performance of the carriers. The optimal concentrations of PVA, SA, diatomite , and Fe 3 O 4 were 10%, 1%, 1%, and 2%, respectively. The obtained carrier achieved a NH 4 + -N removal efficiency of 72.5% after reaction for 12 h, which was 31.8% higher than that with the non-magnetic carrier. The removal of NH 4 + -N was attributed to the cooperative action among non-magnetic ingredients, magnetic ingredients, and microorganisms. The adsorptions of NH 4 + -N by non-magnetic ingredients and Fe 3 O 4 contributed 21.2% and 25.5%, respectively, and microorganism metabolism contributed 53.2% to NH 4 + -N removal. The novel texture structure of the carrier leads to improved mass transfer of nutrients and stable activity of microorganisms. Nitrification followed the first-order reaction for low concentration of effluents and zero-order reaction for high concentration of effluents. The reaction rate increased with increasing initial NH 4 + -N concentration. The magnetic carrier was applicable for a long time. These results show the great potential of the developed carrier for industrial applications.
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JOURNAL OF MOLECULAR STRUCTURE
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2023
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IF 3.8
Di-Ming Chen,Xue-Jing Zhang
Acetylene is an important industrial material for the production of acetaldehyde, acetic acid, benzene, synthetic rubber, synthetic fiber, etc., and its separation from the gas mixtures of C 2 H 2 /CO 2 is of great importance but a challenging task due to their similar physical properties and molecular sizes. In this study, taking the advantage of open-donor sites on the pore surface as the nano-traps for C 2 H 2 molecules, a new microporous metal-organic framework (MOF) formulated as {[C o 3 (OH) 2 (HCOO) 2 (CPT) 2 ](NMF) 2 (MeOH) 3 (H 2 O)} n ( 1 , NMF = N - methylformamide) has been prepared by virtue of a bifunctional organic ligand 4-(4H-1,2,4-triazol-4-yl)benzoic acid (HCPT), which showed discriminating sorption performance toward C 2 H 2 (113 cm 3 /g at 298 K and 1 bar) over CO 2 (33 cm 3 /g) under the same conditions. The different sorption behavior of {[C o 3 (OH) 2 (HCOO) 2 (CPT) 2 ]} n between C 2 H 2 and CO 2 results in a high uptake ration (3.4:1) and IAST selectivity (13) at 298 K and 1 bar. Furthermore, the force-field based grand canonical Monte Carlo (GCMC) simulation results demonstrate that the large electronegativity and polarizability of open O donor sites on the pore surface contribute to its high C 2 H 2 /CO 2 separation performance, leading to its more favorable C 2 H 2 uptakes than CO 2 .
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SCIENCE OF THE TOTAL ENVIRONMENT
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2023
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IF 9.8
Qihui Yu,Hao Liu,Guocheng Lv,Xin Liu,Lijuan Wang,Libing Liao
The contamination of heavy metal lead has a serious impact on the natural environment and organisms. Among various materials for lead removal, animal bone derived hydroxyapatite has received extensive attention. However, there are different opinions among researchers regarding the mechanism of lead removal by hydroxyapatite , possibly due to varying initial lead concentrations used in different studies and lack of accuracy in the study of lead removal mechanisms. In present work, we synthesized a carbon-containing hydroxyapatite (CHAP) through pyrolysis of bovine bone with excellent lead removal efficiency, and further investigated the lead removal mechanism of CHAP under high and low initial lead concentrations by combining XRD Rietveld refinement, FTIR, XPS, HRTEM etc. methods. The results showed that under low initial Pb 2+ concentration condition, the main mechanism of lead removal by CHAP was chemical precipitation (94.1 %), with small contributions of lead complexation with carbon functional groups and cation-π interactions on the amorphous carbon in CHAP, and surface adsorption on the precipitates. Under high initial Pb 2+ concentration condition, chemical precipitation remained the main mechanism (74.68 %), but the contributions of the other three mechanisms increased, and ion exchange appeared in the later stage of the removal process. This study provides new insights on the lead immobilization mechanism by CHAP at different initial Pb 2+ concentrations in water.
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Nano Research
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2023
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IF 9.9
Dong Zhicheng,Xu Yunyun,Wu Can,Chao Jin,Tian Chen,Lin Zhang
Organo-chromium(III) complex is one of the chromium contaminant species, which would transform to high-toxic Cr(VI) during migrating in the environment. Such natural organo-chromium(III) (NOCr) is difficult to remove by traditional degradation or precipitation methods, due to its high stability and solubility. Herein, we demonstrated a novel NOCr removing method by transforming it to certain structures similar to Cr-Fe minerals in nature, through a self-circulating decomplex and immobilization mechanism with nano zero-valent iron (nZVI). Taking chromium glycinate (Cr-Gly) as a probe, nZVI showed a high Cr removal efficiency of 99.4% under ambient conditions. The removal process included three stages of adsorption, decomplexation, and re-immobilization. Cr-Gly was first adsorbed on the surface of nZVI by chemisorption of the oxide shell. Then, the adsorbed Cr-Gly was decomplexed and oxidized to Cr(VI) by ·OH and 1 O 2 , which were generated from molecular oxygen activated by nZVI. Meanwhile, the released Cr(VI) could be in-situ adsorbed and re-reduced to Cr(III), which was further immobilized in form of Cr-O-Fe complex. As the Cr-O-Fe complexing structure was similar to that of Cr-Fe minerals (such as chromohercynite) in nature, this work explored a novel and efficient NOCr removing method that was potential to weaken chromium pollution in the environment.
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BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Jing-jing Guo,Yi-tong Wang,Zhen Fang
Magnetic biochar was synthesized via chelation of Fe 3+ with carboxymethyl cellulose and pyrolysis for covalently immobilizing Eversa® Transform lipase. The magnetic biochar had 75.8 mg/g lipase loading that was 54.1 % higher than that without magnetism. The immobilized lipase achieved 91.3 mg/g lipase loading with 19.2 U/mg lipase activity after optimization. It showed good thermal and acid stability with 82.5 % and 98.2 % relative activity at 45 °C and pH 4, respectively. Its relative activity was 90.8 % after stored for 30 d at 4 °C. After magnetically separated for 10 cycles, it still kept 70.1 % activity due to the strong covalent bonding. The lipase further catalyzed one-pot esterification and transesterification of high acid value oil (38 mg KOH/g) with 95.7 % biodiesel yield and cycled for 10 times at 85.7 % yield. Kinetic study gave the activation energy of 28.7 kJ/mol. The covalently immobilized lipase could find practical applications.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Jing Luo,Chenghao Zhang,Wei Liu,Bingqi Xie,Jisong Zhang
2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) has vital applications in the field of multiphase reaction, especially for the aerobic oxidation of alcohols. Activated carbon (AC) has a porous structure, rigid surface property, and low cost, which was the prominent carrier for TEMPO immobilization to produce the heterogeneous catalyst. However, the brilliant strategy of TEMPO immobilization on AC for long-term aerobic oxidation of alcohols was still challenging. In this work, surface-formylation and reductive amination were conducted to achieve the covalent TEMPO grafting on AC, which opened up a novel routine for surface modification and preparing heterogeneous catalysts based on carbon materials. The catalysts were packed in a micro-packed bed reactor to perform continuous aerobic oxidation of various alcohols. The catalytic performance of benzyl alcohol to benzaldehyde (turnover number: 1160.6, turnover frequency: 99.6 h −1 ) was much superior to those in the literature by using covalently grafted heterogeneous TEMPO catalysts.
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BIOCHEMICAL ENGINEERING JOURNAL
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2019
·
IF 3.371
Jianguo Zhang,Hongli Mao,Mengla Li,Erzheng Su
An enzymatic approach to β-cyclodextrin synthesis attracts the attention of food engineers and chemists because of the broad applications of β-cyclodextrin in the food industry and the demand for a green process for β-cyclodextrin production. Achieving a high recovery of cyclodextrin glycosyltransferase through immobilization and a high proportion of β-cyclodextrin in a product mixture of cyclodextrins would improve the purification process of β-cyclodextrin. In this study, a recombinant cyclodextrin glycosyltransferase (CGTase) was immobilized on polydopamine-Fe 3 O 4 (PDA-Fe 3 O 4 ) nanoparticles in the presence of polyethyleneimine (PEI). After optimization of the preparation of the PDA-Fe 3 O 4 nanoparticles and the CGTase immobilization step, 77.9% of the CGTase was recovered. The immobilized CGTase exhibited good thermal and operational stabilities, and gave a ratio of 88.9% β-cyclodextrin when the reaction temperature and kinetic parameters were optimized. Immobilized CGTase was recycled nine times and retained 19.1% β-activity and 0% γ-activity. This study has established a reliable approach to the high recovery of CGTase during efficient β-cyclodextrin production.
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