TALANTA
·
2017
·
IF 4.162
Li-Ping Jia,Li-Juan Wang,Rong-Na Ma,Lei Shang,Wei Zhang,Qing-Wang Xue,Huai-Sheng Wang
In the present work a highly sensitive and selective aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) based on the hybridization chain reaction (HCR) signal amplification. It was observed that the aptamer of 8-OH-dG could hybridize with the capture DNA immobilized on the gold electrode with a sticky tail left, which initiated the HCR and led to the formation of extended dsDNA structure on the electrode surface. Then the electroactive species ([Ru(NH 3 ) 6 ] 3+ , RuHex) intercalated into the dsDNA grooves to generate the amplified signal. However, in the presence of 8-OH-dG, the aptamer containing G-rich nucleic acid sequences would be induced to form a G-quadruplex structure, which made it impossible to continue the HCR. So the detection signal will significantly decrease. Under the optimal conditions, the peak current of RuHex was linear with the logarithm of 8-OH-dG concentration in the range from 10 pM to 100 μM with the detection limit of 2.5 pM. By integrating the merits of enzyme-free amplification power of the HCR and the inherent high sensitivity of the electrochemical technique, the prepared aptasensor not only showed high sensitivity for the detection of 8-OH-dG, but also exhibited good selectivity against to the uric acid , an important interferent in the urine sample. Particularly, the aptasensor was applied to detect 8-OH-dG in urine samples with satisfactory results.
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高影响因子
SENSORS
·
2017
·Vol. 17
·期 6
·1235
·
IF 2.677
Yao Zhao,Xueyong Wei,Niancai Peng,Jiuhong Wang,Zhuangde Jiang
Uric acid (UA) is a kind of purine metabolism product and important in clinical diagnosis. In this work, we present a study of ZnS nanostructures-based electrochemical and photoelectrochemical biosensors for UA detection. Through a simple hydrothermal method and varying the ratio of reaction solvents, we obtained ZnS nanomaterials of one-dimensional to three-dimensional morphologies and they were characterized using field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). To fabricate the UA biosensor and study the effect of material morphology on its performance, ZnS nanomaterials were deposited on indium tin oxide (ITO) conducting glass and then coated with uricase by physical absorption. Three kinds of working electrodes were characterized by cyclic voltammetry method. The effect of material morphology on performance of UA detection was investigated via amperometric response based electrochemical method based on enzymatic reaction. The ZnS urchin-like nanostructures electrode shows better sensitivity compared with those made of nanoparticles and nanoflakes because of its high surface-area-to-volume ratio. The photoelectrochemical method for detection of UA was also studied. The sensitivity was increased 5 times after irradiation of 300 nm UV light. These results indicate that ZnS nanostructures are good candidate materials for developing enzyme-based UA biosensors.Keywords:biosensor;enzyme;nanostructures;ZnS;UA
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高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Yi Xin,Wu Yuxuan,Tan Guoxin,Yu Peng,Zhou Lei,Zhou Zhengnan,Chen Junqi,Wang Zhengao,Pang Jinshan,Ning Chengyun
Traced dopamine (DA) detection is critical for the early diagnosis and prevention of some diseases such as Parkinson’s, Alzheimer and schizophrenia. In this research, a novel self-supporting three dimensional (3D) bicontinuous nanoporous electrochemical biosensor was developed for the detection of dopamine by Differential Pulse Voltammetry (DPV). This biosensor was fabricated by electrodepositing palladium nanoparticles (Pd) onto self-supporting nanoporous gold (NPG) wire. Because of the synergistic effects of the excellent catalytic activity of Pd and novel structure of NPG wire, the palladium nanoparticles decorated NPG (Pd/NPG) biosensor possess tremendous superiority in the detection of DA. The Pd/NPG wire biosensor exhibited high sensitivity of 1.19 μA μΜ −1 , broad detection range of 1–220 μM and low detection limit up to 1 μM. Besides, the proposed dopamine biosensor possessed good stability, reproducibility, reusability and selectivity. The response currents of detection in the fetal bovine serum were also close to the standard solutions. Therefore the Pd/NPG wire biosensor is promising to been used in clinic.
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高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2017
·Vol. 61
·期 12
·1700332
·
IF 4.323
Xiaoyao Ma,Yuan Zhang,Zengyong Wang,Yunbing Shen,Man Zhang,Quandeng Nie,Yuanyuan Hou,Gang Bai
Scope Ursolic acid (UA) is a pentacyclicterpenoid carboxylic acid that is present in a wide variety of plant foods. There are many beneficial health effects that are attributed to the properties of UA. However, the specific cellular targets of UA and the mechanism underlying downstream signal transduction processes linked to the anti-inflammation pathway have not been thoroughly elucidated to date. Methods and results Chemical biology strategies such as target fishing, click reaction synthesis of a UA probe and molecular imaging were used to identify potential target proteins of UA. Cysteinyl aspartate specific proteinase 3 (CASP3) and its downstream signaling pathway were verified as potential targets by molecular docking, intracellular enzyme activity evaluation and accurate pathway analysis. The results indicated that UA acted on CASP3, ERK1 and JNK2 targets, alleviated inflammation-associated downstream multiple signal transduction factors, including ERK1, NF-κB and STAT3, and exhibited anti-inflammation activities. Conclusion As a natural dietary supplement, UA demonstrated anti-inflammation activity via inhibition of CASP3 and shows the potential to improve the therapy effect of several inflammation-associated diseases.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-10
·
IF 5.3
Ke Wenrui,Kuang Guochen,Gu Chuangqi,Wang Junhao,Jiang Xiao,Zhu Rongjiao,Wang Haiyuan,Zhang Zhen
A Cr-doped VO 2 nanobelt (Cr/VO 2 ) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO 2 for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H 2 O 2 ) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO 2 exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO 2 can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications. Graphical abstract
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高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yu-Ting Xue,Zhencheng Chen,Xin Chen,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
A non-enzymatic glucose sensor was successfully prepared by the electrodeposition methods assembling of Zn-metal-organic frameworks/multi-walled carbon nanotubes (Zn-MOF/MWCNTs) on gold electrode (GE). The sensor showed better catalytic oxidation performance for glucose at very low potential (0.20 V) compared to the bare GE (0.30 V), because the modified complex can effectively increase the active site and reaction contact area. The sensor exhibited a wider linear detection range of 0.020–8.14 mM with a detection limit of 0.037 mΜ. In addition, the electrochemical response of the sensor to glucose showed good selectivity, which was further verified in the complex serum environment by the standard addition recovery method. The recovery of the sensor was in the range of 98.52–102.65%, with a relative standard deviation (RSD) of no more than 8.00%, showing the potential applications in real testing.
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Materials Today Chemistry
·
2024
·
IF 6.7
Nana Tang,Yanping Wei,Quanguo He,Shuting Shi,Chuanqin Zhou,Aiting Chen,Jun Liu
A novel and facile electrochemical determination of Sertraline at nanomolar concentration using bimetallic MXene NbTiCT x and MWCNTs hybridized electrodes as sensing platforms is demonstrated. NbTiCT x /MWCNTs composites were produced by etching NbTiAlC to obtain NbTiCT x , and ultrasonically mixing this with MWCNTs. An electrochemical sensor based on NbTiCT x /MWCNTs nanocomposite modified (NbTiCT x /MWCNTs/GCE) was constructed, for use in the sensitive detection of Sertraline (SER). The successful preparation of NbTiCT x /MWCNTs and the electrochemical performance of the electrodes were verified by Scanning electron microscopy, X-ray diffraction, cyclic voltammetry and electrochemical impedance characterization methods. The experimental results show that NbTiCT x /MWCNTs/GCE can increase the SER oxidation peak current, with two good linear relationships (R 2 = 0.9910 and 0.9967) between the current and the concentration of SER (5.0–10.0 μM and 10.0–100.0 μM), with a detection limit of 0.16 μM (S/N = 3). The sensor has good selectivity, repeatability, reproducibility and stability, and enables the sensitive determination of SER in human serum and urine samples of clinical biological fluids. It is believed that this work is significant and is of broad interest for work in innovative material development and biomedical applications.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fan Shi,Jiaqi Geng,Behisht Ara,Baoli Wang,Xiaojiao Li,Qiantao Ma,Lisi Wang,Wanting Fu,Mansoor Ahmad,Wei Sun
Electrochemical sensor is a sensitive and fast method to test hydrogen peroxide (H 2 O 2 ) released from cancer cells. To map a clear understanding of the mechanisms during drug treatment of cancer, it is necessary to in-situ and real-time monitor of H 2 O 2 content in the release process from cancer cells. Herein, N-doped helical carbon nanotubes (HCNTs) coated manganese oxide (MnO) nanoparticles (MnO@HCNTs) were prepared using a high-temperature molten salt method. The ratio of Mn salt to 2-methylimidazole was adjusted to optimize the Mn 2+ /Mn 3+ ratio of the active center. The MnO@HCNTs were further modified on a polydimethylsiloxane (PDMS) based electrode printed by screen printing technique to build a flexible electrochemical sensor (MnO@HCNTs/PDMS). The presence of graphitic N increases the surface electron density, thereby enhancing the electrical conductivity of MnO@HCNTs/PDMS. Simultaneously, graphitic N triggers electronic arrangement in the Mn, leading to an increase in Mn-N coordination. Furthermore, the adsorption performance of MnO@HCNTs/PDMS for H 2 O 2 increases with more N content, which may also enhance the electrocatalytic activity towards H 2 O 2 . The sensitivity of this sensor for H 2 O 2 analysis was observed as 636.9 μA μM −1 cm −2 with a limit of detection as 2.6 nmol L −1 in a linear response range from 10.0 nmol L −1 to 1000.0 nmol L −1 and a fast response time of 1.2 s. Finally, the flexible MnO@HCNTs/PDMS was combined with a smart sensing system, which displayed the ability for highly sensitive real-time monitoring of drug-stimulated H 2 O 2 that released from lung cancer cells A549 under the phorbol ester stimulation.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lu Fang,Yi Zhang,Ye Liu,Jialong Shou,Hongying Liu,Lihua Li
Levodopa (L-Dopa) is a widely prescribed drug for treating Parkinson's disease (PD), however, due to its motor complications, dosage management is required during clinical treatment. Uric acid (UA) is an important antioxidant in the body and is believed to have a defensive effect on the development and progression of PD. Therefore, accurate measurement of L-Dopa and UA in PD patients is of great significance. In this study, a flexible electrochemical sensor based on carbon nanotube (CNT) fibers was developed for individual and simultaneous determination of L-Dopa and UA. The electrochemical behavior and detection performance of the sensor were studied by cyclic voltammetry and differential pulse voltammetry. The CNT fibers (CNTF) electrode exhibited a wide detection range of 0.16–2.08 mM and 0.08–0.96 mM for L-Dopa and UA, as well as a high sensitivity of 0.7142 μA μM −1 cm −2 and 1.33 μA μM −1 cm −2 , respectively. In addition, the sensor also exhibited good anti-interference, repeatability and bending stability, and has been successfully applied to the detection of L-Dopa and UA in bovine serum samples.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Haifeng Zhang,Zhaoxin Li,Shengtian Sang,Liang Yin
As the number of people with diabetes continues to increase, the use of high-performing blood glucose testing devices for blood glucose management is critical. Therefore, a flexible micronedle sensor (MNs sensor) based on flexible micronedle array structure (MNs array) is proposed in this study, inspired by the ultra-fast water absorption characteristics of straw, which can be used to quickly, conveniently and noninvasculatively detect glucose concentration in human interstitium fluid (ISF). The MNs sensor is the composite of flexible MNs array and highly sensitive detection electrode. The MNs sensor has excellent mechanical properties, the maximum pressure is 69.2 N (4 times the average), the compressive strength is 88.13 MPa (1.88 times the average), and the fracture compression ratio is 194.7 %(3.36 times the average). These properties enable MN sensors to penetrate the stratum corneum in complete form while extracting ISF quickly and consistently. Meanwhile, the MN sensor has the highly sensitivity (1826.75nA mM −1 cm −2 , 4.72 times the average value). It has a wider detection range (1 mM-30 mM) and faster detection (30 s) than existing MN glucose detectors. In addition, the MN sensor has excellent anti-interference performance and can accurately detect glucose concentration in the case of multiple disturbances. This work lays a foundation for the development of a new painless, real-time monitoring and high accuracy blood glucose detection system.
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Advanced Science
·
2025
·
IF 14.1
Zhenru Li,Li-Peng Sun,Yanzhen Tan,Zhiwei Wang,Xiao Yang,Tiansheng Huang,Jie Li,Yi Zhang,Bai-Ou Guan
Flexible wearable biosensors have emerged as a promising tool for tracking dynamic glycemic profiles of human body in diabetes management. However, it remains a challenge to balance the shrunken device space and multiple redundant sensing arrays for further advancement in miniaturization of multimodal sensors. Herein, this work proposes an entirely new optoelectronic hybrid multimodal optical fiber sensor which is composed of laser patterning of polydimethylsiloxane (PDMS) to form laser-induced graphene (LIG) as the interdigital electrodes, and a long period grating (LPG) prepared from an optical microfiber encapsulated into the PDMS modulated by periodical structure of LIG electrodes. This operation can simultaneously integrate two heterogeneous sensing mechanisms, optical and electrical, into a single sensor in a compact manner. Combining the LIG electrode with conductive hydrogel, a flexible glucose biosensor based on electrical mechanism is constructed by loading glucose oxidase into the hydrogel. Meanwhile, the microfiber LPG can also be served as a spectroscopically available sensor for biomechanical monitoring. Optical and electrical sensors can work simultaneously but independently of each other, particularly in the scene of wound healing for rat model and movement for human exercise. This platform represents a pivotal step toward multifunctional sensors that enable measurements of biomechanical information and glucose.
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高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Chuanqin Zhou,Yingyu Sima,Yiyong Wu,Yanping Wei,Du Tuo,Shuting Shi,Aiting Chen,Nana Tang,Quanguo He,Wu Wang
Clozapine is vital for managing schizophrenia and other psychiatric disorders. As a potent medication with risks of severe side effects, accurate detection ensures effective treatment monitoring, patient safety, and optimization of therapeutic outcomes. Herein, niobium carbide two-dimensional transition metal carbides (Nb 2 CT x MXene) was prepared by etching niobium aluminum carbide (Nb 2 AlC), which was next partially oxidized by a hydrothermal method to niobium oxide (Nb 2 O 5 )/Nb 2 CT x and ultrasonically bound to carboxylated multi-walled carbon nanotubes (MWCNTs-COOH). It was modified onto a glassy carbon electrode for the detection of the antipsychotic drug clozapine ingested by humans. The morphology and electrochemical properties of MXene-based nanocomposite electrode were verified by scanning electron microscopy, X-ray diffractometry, Raman, cyclic voltammetry and electrochemical impedance characterization methods. The results showed that electrode responded well to clozapine solutions, with good linearity ( R 2 = 0.9979 and 0.9995) of the peak currents with clozapine concentrations (0.01–1.0 μM and 1.0–10.0 μM) and that this sensor had good immunity, repeatability, reproducibility and stability. The limit of detection was 8.38 nM and the recovery was 92.5 % ~ 106.5 % in real serum and urine samples. The MXene-based sensors offer excellent analytical performance and hold promise for cost-effective generalisation to the detection of nanomolar clozapine in a variety of clinical applications.
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