Analytical Methods
·
2024
·Vol. 16
·期 16
·2472-2477
·
IF 2.7
Chenhao Zhao,Xingdan Wang,Jing Wu,Yeli Hu,Qiwei Zhang,Qi Zheng
Sialic acids are a family of monosaccharides that share a nine-carbon backbone and a carboxyl group. A recent derivatization method based on 3-nitrophenylhydrazine (3-NPH) provides a mild chemical labeling technique for biomolecules containing carbonyl or carboxyl groups. In this study, we utilized 3-NPH to label sialic acids via a two-step derivatization process. The derivatized species can produce a common reporter ion corresponding to C1–C3 with two labels, and a fragment differentiating between Neu5Ac, Neu5Gc, and KDN. This method is compatible with O-acetylated sialic acids and provides high sensitivity to Neu5Gc and KDN, and since the utilization of dual labeling significantly enhances the hydrophobicity of derivatives, it can effectively mitigate matrix effects when combined with parallel reaction monitoring technology. Negative-ion tandem mass spectrometry (MS/MS) analysis reveals a distinctive fragmentation profile for the 4-O-acetylated species, while the other sialic acids yield similar MS/MS spectra with a high abundance of reporter ions. Using the reporter ion as a transition, this analytical strategy is effective for analyzing complex biological samples. For example, it was successfully employed to quantify sialic acids in the intestinal tissues of several carp species, demonstrating its potential in sialylation research.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2024
·
IF 8.1
Xiang Liu,Ailing Zhang,Hao Yu,Liang Chen,Lei Zhang,Yong Zhao,Jialu Li,Weiqi Zhang,Zhiting Zhou,Yongyan Zhou,Yuanyuan Wang,Jian Zhen Ou
Alkaline water electrolysis is one of the primary drivers of hydrogen energy development, and anion exchange membranes (AEMs) play a dual role in ensuring both conductivity and safety. However, traditional polymer AEMs have a wide pore size distribution and poor chemical stability, making it difficult to achieve a long-term balance between conductivity and safety of the water electrolysis system. Here, we select inorganic two-dimensional multilayer graphene oxide (GO) membranes as AEMs, using carboxylated wrinkled graphene (WG) and ethylenediamine (EDA) to create a cation-modified porous EDA-WG/GO (E-W/G) composite membrane with a 3 nm pore size. The enlarged channel size and enhanced hydrophilicity improve OH − permeability compared to the pristine GO membrane, while the strengthened hydration layer acts as a barrier to hydrophobic gases for O 2 /H 2 separation. The results show that the prepared E-W/G membrane exhibits superior current density (600 mA cm −2 ) and gas impermeability (gas purity 99.99%) compared to the commercial Fumasep FAA-3-50 membrane (590 mA cm −2 and 99.81%, respectively). Furthermore, after continuous testing for 168 h in high-temperature and alkaline environments, the E-W/G membrane maintained conductivity comparable to its initial state and showed enhanced gas impermeability. Our strategy provides new insights into the design of high-performance AEMs and is expected to contribute to the advancement of the hydrogen energy industry.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Liuying Zhu,Minghui Wang,Hongyan Li,Ting Luo,Zeyuan Deng,Jing Li,Liufeng Zheng,Bing Zhang
3′-Sialyllactose (3′-SL), a major acidic oligosaccharide found in human milk, has been investigated to improve cognitive-enhancing effects with 3 weeks old C57BL/6 mice by administering 3′-SL orally at a dose of 350 mg/kg/day for 6 weeks. Behavioral tests indicated that supplementation with 3′-SL promoted cognitive and memory development in young mice. Through interaction network and coenrichment analysis, nine differentially expressed genes (DEGs) related to memory and cognition were identified and localized in the hippocampal tissue of mice. The intervention of 3′-SL significantly increased the metabolism of sialic acid in mouse hippocampal tissue and promoted the expression of learning-related genes (p < 0.05). Notably, it increased the expression of genes associated with neural cell adhesion molecule (NCAM, p < 0.05), glutamate receptors, and fibroblast growth factor receptor (FGFR, p < 0.05). This suggests that 3′-SL may elevate polysialylated NCAM (PSA-NCAM) levels, which could subsequently interact with FGFR and glutamate receptors, thereby enhancing synaptic growth and plasticity. Additionally, 3′-SL altered the composition of the mouse intestinal microbiota. The synergistic action of gut microbiota and intestinal sialidase promoted the production of free sialic acid, providing essential nutritional elements for the development of the brain’s nervous system. In conclusion, our findings provide new insights into the promoting effect of 3′-SL on cognitive development in growing mice and elucidate its molecular mechanisms.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Peng-Xing Xie,Qing-Yuan Zhao,Haojie Dong,Tao Long,Meng-Sha Ding,Bin Xie,Xiao-Feng Wang,Wei Ling,Mengting Liu,Xiong-Wei Wu,Peng-Fei Wang,Xian-Xiang Zeng
Delivery of high energy density in polyanion compound-Na 3 V 2 (PO 4 ) 3 based on three-electron redox is still challenging because of their controversial multi-electron reaction mechanism and unsatisfactory electrochemical reversibility. Herein, the reversible V 4+ /V 5+ redox ( ca. 4.0 V) could be successfully activated in Na 4 VFe(PO 4 ) 3 cathode material through a Na-rich strategy, demonstrating a reversible specific capacity of 154.7mAh g −1 at 0.1C and 90.3mAh g −1 after 800 cycles at 20C, with 80 % of the initial capacity retained. The entire electrochemical reaction undergoes a highly recoverable phase transition by in-situ X-ray diffraction. Impressively, the Na-rich strategy could not only allow Na2 site to be occupied to activate the V 4+ /V 5+ redox pair confirmed by X-ray absorption near-edge structure spectroscopy, but also enable Na + transportation across the generated vacancies rather than synergistic Na + diffusion. This work provides rational design strategy of multi-electron transfer reaction in high-energy sodium-ion batteries.
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Metals
·
2024
·Vol. 14
·期 12
·1453
·
IF 2.6
Bo Pei,Xin Qiao,Que Huang,Changcheng Liu,Mengna Shi,Xiaomei Jiang,Feng Li,Li Guo
Na3V2(PO4)3(NVP) is considered to be a promising cathode material for sodium-ion batteries (SIBs). Ion doping can effectively improve its structural deformation, poor conductivity, and electrochemical performance. However, the research on the effect of ion doping on the thermal stability of NVP is still limited. In this paper, Mg/Ti co-doped and Mn/Ti co-doped modified NVP with carbon nanotubes (CNTs) (MgTi@ CNTs and MnTi@CNTs) were prepared, respectively, and X-ray diffraction (XRD) results proved that MgTi@CNTs and MnTi@CNTs have good structural stability and crystallinity. The electrochemical performance indicates that the dual strategy ofp-n-type co-doping and CNT coating provides superior sodium storage performance, enhancing both electronic conductivity and ion diffusion. Secondly, based on the safety point of view, the thermal stability ofp-n-type ion-doped NVP and its mixed system with electrolyte in a charged state was studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and accelerated calorimeter (ARC). The results show that the optimized MgTi@CNTs and MnTi@CNTs electrodes exhibit excellent thermal stability in the absence of electrolytes, indicating their high intrinsic safety. However, it is worth noting that in the electrode/electrolyte system,p-n-type ion-doped NVP have higher reactivity with the electrolyte, and their comprehensive thermal safety is lower than that of NVP. Therefore, in practical applications, it is necessary to comprehensively consider the thermal stability of the material and the thermal safety of its mixed system with the electrolyte. This paper provides a data basis for the practical application of NVP in SIBs.
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JOURNAL OF VIROLOGY
·
2025
·
IF 3.8
Xiaohan Chen,Yipeng Pang,Fructueux Modeste Amona,Zilu Liu,Fang Wang,Yuan Liang,Jiachen Yang,Wanhan Zhang,Xingtang Fang,Xi Chen
Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious pathogen that causes substantial economic losses in the global swine industry, primarily by impairing reproductive performance and respiratory health. However, current therapeutic approaches remain limited in effectively controlling PRRSV infection. Here, we present self-assembled cyanidin-3-O-glucoside (C3G)-based nanoparticles (C3G-Cs-SeNPs) as a promising antiviral agent against PRRSV infection. These nanoparticles demonstrated excellent stability and biocompatibility, with minimal cytotoxicity in Marc-145 cells. C3G-Cs-SeNPs significantly reduced ROS levels in PRRSV-infected cells, enhancing the antioxidant capacity and mitigating oxidative stress. In addition, they effectively inhibited key stages of the PRRSV lifecycle, including viral internalization and replication, leading to a marked decrease in viral proliferation. Moreover, C3G-Cs-SeNPs alleviated ferroptosis by restoring redox homeostasis through enhanced antioxidant enzyme activity. Mechanistically, these nanoparticles potentially activate the SIRT1/Nrf2/HO-1 signaling pathway, which plays a critical role in regulating inflammation and ferroptosis in PRRSV-infected cells. These findings suggest that C3G-Cs-SeNPs could serve as a novel therapeutic approach to modulate inflammation, ferroptosis, and viral replication in respiratory viral infections. Our study highlights the therapeutic potential of C3G-Cs-SeNPs in combating PRRSV infection, with important implications for viral infection management and respiratory disease treatment.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dehao Jia,Luyang lv,Weijia Sun,Tian Tian,Binnan Shi,Nuo Zhang,Lei Yang,Qin Wei
CA15–3 serves as a tumor marker for breast cancer, and its sensitive detection is crucial for monitoring the disease and managing its treatment. In this study, an electrochemiluminescence (ECL) sensor for the detection of CA15–3 was prepared by using zirconium metal-organic frameworks (Zr-TCBPE) as an ECL emitter and Pt and Pb nanoparticles grown on graphene (rGO-PtPd) as substrate materials. The formation of a ligand bond between the 1,1,2,2-tetra(4-carboxylbiphenyl) ethylene (H 4 TCBPE) and Zr restricted the intramolecular motion of H 4 TCBPE, thereby suppressing nonradiative transitions and triggering a strong coordination-induced ECL enhancement effect. Simultaneous low-potential oxidation using N 2 H 4 as a co-reactant further significantly enhanced the ECL intensity. This method exhibited a wide detection range of 0.1 mU/mL to 500 U/mL, with a detection limit of 0.026 mU/mL. It demonstrated high accuracy and satisfactory reproducibility, indicating that the coordination-induced ECL sensing approach was highly promising for the analysis of disease biomarkers.
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Crystals
·
2025
·Vol. 15
·期 7
·623
·
IF 2.4
Xuekai Lan,Bairui Chen,Huatao Tang,Changzhi Yin,Bin Tian,Wen Lei
Microwave dielectric ceramics are indispensable in modern communication technologies, playing a pivotal role in components such as filters, oscillators, and antennas. Among these materials, ZnAl2O4ceramics have garnered attention for their excellent quality factor (Q × f) and low dielectric constant (εr). However, their high sintering temperature (~1650 °C) limits practical applications. This study investigates ZnAl2-x(Zn0.5Si0.5)xO4(ZAZS) (x= 0.1–0.9) ceramics, where [Zn0.5Si0.5]3+substitutes Al3+, to reduce sintering temperature while maintaining high-performance microwave dielectric properties. ZAZS ceramics were synthesized via the solid-state reaction method and characterized for their structural, morphological, and dielectric properties. X-ray diffraction analysis confirmed the formation of a single-phase solid solution up tox= 0.8, with minor secondary phases appearing atx= 0.9. The substitution increased lattice parameters and enhanced material densification, as observed through SEM and relative density calculations. Microwave dielectric measurements showed that ZAZS ceramics achieved a maximumQ × fof 20,200 GHz and aτfvalue reduced to −62 ppm/°C atx= 0.8, whileεrdecreased from 7.90 to 6.98. Bond-valence calculations reveal that the reduction of the average Al/Zn/Si–O bond valence weakens octahedral rigidity, systematically tuningτftoward zero. These results demonstrate that ZAZS ceramics, with a reduced sintering temperature of 1400 °C, exhibit excellent potential for application in low-loss microwave devices.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Yuxin Lin,Luyao Wang,Sining Meng,Jianjia Liu,Xinyu Li,Wenjuan Wang,Miaoyang Hu,Xi Wang,Weixia Duan,Shangcheng Xu,Jun Wang,Rong Gao
Bisphenol F (BPF), one of the major substitutes for bisphenol A (BPA), is increasingly used in pharmaceuticals, food packaging, and chemical manufacturing. Emerging evidence suggests its potential neuropsychiatric risks. However, the underlying mechanisms remain poorly characterized. Thus, the present study was performed to investigate the roles of the kynurenine (KYN) pathway in BPF induced anxiety- and depression-like behaviors, especially the keys roles of the toxic metabolite 3-hydroxykynurenine (3−HK). The mice were administrated with BPF (10, 40, 160 μg/kg/day) for 30 consecutive days, and the behaviors as well as the changes of the KYN pathway were examined. Noteworthily, even low-dose of BPF exposure (40 μg/kg/day) elicited pronounced anxiety- and depression-like behaviors, molecularly shown as the synaptic marker proteins (PSD95, SYN) and dendritic spine proteins (Drebrin) loss in the prefrontal cortex and hippocampus, along with impaired synaptic connection. The UHPLC-MS/MS metabolomic profiling revealed the aberrant metabolism of the KYN pathway, as shown by the elevated neurotoxic metabolite 3-HK and decreased neuroprotective metabolite kynurenic acid (KynA). Mechanistic analyses demonstrated that the 3-HK rate-limiting enzyme KMO was upregulated while the KynA rate-limiting enzyme KAT2 was downregulated in mouse brain. Notably, pharmalogical inhibition of KMO (GSK180) or blockage kynurenine generation with the TDO2 inhibitor (680C91) substantially ameliorated the BPF induced anxiety- and depression-like behaviors and synaptic impairments. Collectively, the present study unravels a previously unrecognized the BPF induced anxiety- and depression-like behaviors in mice via KMO and 3-HK increase. Therefore, targeting the KMO may provide novel interventive strategy for BPF induced neurobehavioral disorders.
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JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2025
·
IF 2.5
Xiaoli Liu,Shan Wang,Chuangfu Kuang,Yuwen Deng,Shuaicai Yuan,Juying Zou
We aim to explore the key metabolic components and underlying mechanisms of the Bushen Huoxue Formula (BH) in treating Osteoarthritis (OA). The mouse knee OA model was constructed using the destabilization of the medial meniscus method. OA mice were orally administered the BH. Mouse cartilage damage was assessed. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS), network pharmacology analysis and molecular docking were employed to analyze the serum metabolite components and target protein of BH. After lipopolysaccharide (LPS) treatment, different concentrations of β-Estradiol 3-acetate were added to primary chondrocytes. Flow cytometry was utilized for detecting cell apoptosis. The Ubiquitin-Specific Protease 13 (USP13)/Toll-like Receptor 4 (TLR4)/Myeloid Differentiation Primary Response Protein 88 (MYD88)/NF-κB pathway and the TLR4 ubiquitination levels were assessed using immunological quantification and biochemical methods. Relative to normal mice, OA mice exhibited decreased knee joint cartilage thickness and increased inflammatory damage. BH treatment reversed these effects. Furthermore, BH enhanced TLR4 ubiquitination. Estradiol acetate was identified as the metabolic component of BH that alleviates OA. Estradiol acetate and its subtype molecule β-Estradiol 3-acetate could bind to the USP13 protein. The β-Estradiol 3-acetate concentration-dependently decreased the elevated levels of USP13, TLR4, MYD88, p-p65/p65 in chondrocytes induced by LPS, while increasing the TLR4 ubiquitination. β-Estradiol 3-acetate reversed LPS-induced chondrocyte apoptosis and elevation of inflammatory factors. Moreover, USP13 overexpression abolished the protective effects of BH and β-Estradiol 3-acetate against LPS-induced chondrocytes. In Conclusion, the BH metabolite β-Estradiol 3-acetate promotes TLR4 ubiquitination to relieve inflammation and apoptosis in OA chondrocytes by inhibiting USP13.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Zhihao Jiang,Zihao Han,Jianwei Dao,Tiqun Wei,Zhenglin Jiang,Liang Yang
Narrowband red-emitting phosphors excited by near-ultraviolet (near-UV) light can significantly improve the R 9 and are promising for white light-emitting diodes (WLEDs). In this work, Li 6 Y 1-x (BO 3 ) 3 :xEu 3+ is first reported as a novel narrowband red phosphor, which exhibits a high R 9 value (83.8) under near-UV chip excitation for fabricating WLEDs with high color rendering index (CRI). X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) confirmed that Eu 3+ successfully partially substituted Y 3+ in the Li 6 Y(BO 3 ) 3 lattice, and all samples maintained a monoclinic P 2 1 /c structure without impurity phases. Under 396 nm near-UV excitation, Li 6 Y 1-x (BO 3 ) 3 :xEu 3+ phosphors showed intense red emission at 616 nm (attributed to the Eu 3+ 5 D 0 → 7 F 2 transition) with a full width at half maximum (FWHM = 4.8 nm), and this narrowband feature directly contributes to the improved R 9 . The optimum Eu 3+ doping concentration was 0.07; at this concentration, the red-orange ratio (the intensity ratio of 5 D 0 → 7 F 2 to 5 D 0 → 7 F 1 transitions) reached a maximum value of 4.46, indicating Eu 3+ is in a low-symmetry crystal field with high red emission purity. The luminescent intensity at 423 K remained 68.9 % of that at room temperature, with an activation energy of 0.186 eV, demonstrating excellent high-temperature stability. When the optimal phosphor was mixed with commercial BaMgAl 10 O 17 :Eu 2+ blue and (Ba, Sr) 2 SiO 4 :Eu 2+ green phosphors and packaged on a 395–400 nm chip, the fabricated WLED exhibited a high CRI (R a = 87) and a suitable correlated color temperature (CCT = 5753 K). The results demonstrate that Li 6 Y(BO 3 ) 3 :Eu 3+ is a very promising red phosphor candidate for high-CRI solid-state lighting
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Yuanqi Lv,Lin Chen,Haizhou Wu,Xinglian Xu,Guanghong Zhou,Beiwei Zhu,Xianchao Feng
The quality of meat products is influenced by protein oxidation. Understanding the effects of antioxidant polyphenols and oxidizing lipoperoxides on myofibrillar proteins is essential for controlling the quality of meat products. This study was designed to investigate the epigallocatechin-3-gallate (EGCG)-mediated formation of myofibrillar protein emulsion gels under malondialdehyde (MDA)-induced oxidative stress. Low MDA concentrations (3 and 6 mM) improved the water-holding capacity, strength, elasticity, and emulsifying properties of gels due to the enhanced protein-protein interactions via MDA. The addition of EGCG reduced the overall properties of the emulsion gel due to further modification of the proteins. At a high concentration of MDA (12 mM), there was excessive cross-linking and unfolding of myofibrillar proteins, which led to a compact gel structure, and reduced the overall properties of the gel. In this situation, the addition of EGCG protected myofibrillar proteins from modifications induced by MDA and improved the quality of emulsion gels.
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