NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 11
·8537-8543
·
IF 3.201
Zhifeng Zhang,Wenwen Wang,Zhentan Lu,Ke Liu,Qiongzhen Liu,Dong Wang
Poly(glycidyl methacrylate)-b-polystyrene (PGMA-b-PS) block copolymers were synthesized by two steps with 1,1-diphenylethylene (DPE) as the chain transfer reagent. The molecular weights and chemical structures of block copolymers were characterized by GPC, FTIR and NMR analyses. Then PGMA-b-PS fibers were fabricated under a shear field using methanol as the antisolvent and glycerin as the shear medium. The shear speed, the concentration of block copolymer solution and the ratios of methanol to glycerin were controlled and regulated. The morphologies of block copolymer fibers were investigated by SEM. Finally, immobilization of bovine hemoglobin on block copolymer fibers was carried out which has potential applications in blood substitutes.
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TALANTA
·
2015
·
IF 3.545
Youming Shen,Youyu Zhang,Meiling Liu,Xiaoyin Liu,Huan Guo,Xiangyang Zhang,Chunxiang Zhang,Haitao Li,Shouzhuo Yao
In this work, a novel sulfur-containing compound with aldehyde groups was synthesized, which was used to immobilize antibodies through the covalent bonding of the aldehyde groups with amino groups, in which no additional chemical cross-linker is required. Human IgG was used as a model analyte to fabricate the electrochemical immunosensor. Using the proposed immunosensor, IgG was detected within the range from 0.01 to 25 ng mL −1 with a detection limit of 0.003 ng mL −1 obtained by 3 S/N. The simple electrochemical immunosensor had a good specificity, stability and reproducibility.
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PubMed
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RSC Advances
·
2014
·Vol. 5
·期 10
·7304-7310
·
IF 3.708
Hong Yang,Ming Xu,Ling-Xiang Guo,Hao-Fan Ji,Jun-Yu Wang,Bao-Ping Lin,Xue-Qin Zhang,Ying Sun
This manuscript presents a facile thiol–ene photo-click chemistry method to prepare magnetic stir bar-encapsulated polysiloxane-based organocatalyst gels under benign conditions, and develops a Stir Bar-Encapsulated Catalysis (SBEC) technique. Through thiol–ene addition chemistry, we graft olefin-terminated organocatalysts (i.e. MacMillan catalyst, proline catalyst, and N-heterocyclic carbene catalyst) onto poly[3-mercaptopropylmethylsiloxane], which is further photo-crosslinked to coat the embedded magnetic stir bar. The prepared magnetic stir bar-encapsulated polysiloxane-based organocatalyst gels can be put into reaction flasks to perform stirring and catalysis functions at the same time. The most important benefit of SBEC technique is to infinitely simplify the catalyst/product separation procedure by using a simple stir-bar-retriever, even without any precipitation/filtration steps. The catalytic performances of three different organocatalyst gels applied in asymmetric Diels–Alder reaction, asymmetric aldol reaction and benzoin condensation reaction respectively are also examined herein.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 2.467
Dong Yang,Xueyan Wang,Jiafu Shi,Xiaoli Wang,Shaohua Zhang,Pingping Han,Zhongyi Jiang
Herein, the reduced graphene oxide–Fe 3 O 4 (rGO–Fe 3 O 4 ) nanocomposites are synthesized by the simultaneous reduction of graphene oxide (GO) and in situ deposition of Fe 3 O 4 nanoparticles ( ca. 20 nm) enabled by Fe 2+ ions. The rGO–Fe 3 O 4 nanocomposites integrate the magnetic property of Fe 3 O 4 nanoparticles and the large specific surface area of rGO nanosheets. Catalase (CAT), as a commonly used enzyme, can be efficiently immobilized on the rGO–Fe 3 O 4 nanocomposites through physical adsorption. The CAT loading capacity is as high as 312.5 ± 12.6 mg g −1 , while the activity recovery of CAT can be high up to nearly 98%. The strong magnetic response of immobilized CAT ensures its easy separation from the reaction system when an external magnetic field is applied. Owing to the hydrophobic and hydrogen bonding interactions between enzyme and the support, the immobilized CAT exhibits zero leaching, desirable stability and excellent reusability.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2014
·
IF 0.872
Jingyun Wang,Fangling Ji,Jishuang Xing,Shuang Cui,Yongming Bao,Wenbo Hao
A block copolymer of 2-dimethylaminoethyl methacrylate (DMAEMA) and glycidyl methacrylate (GMA) was grafted onto the surface of magnetic nanoparticles (Fe 3 O 4 ) via atom transfer radical polymerization . The resultant PGMA-b-PDMAEMA-grafted-Fe 3 O 4 magnetic nanoparticles with amino and epoxy groups were characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, thermo-gravimetric analysis, and scanning electron microscopy. Lipase from Burkholderia cepacia was successfully immobilized onto the magnetic nanoparticles by physical adsorption and covalent bonding. The immobilization capacity of the magnetic particles is 0.5 mg lipase per mg support, with an activity recovery of up to 43.1% under the optimum immobilization condition. Biochemical characterization shows that the immobilized lipase exhibits improved thermal stability, good tolerance to organic solvents with high lg P , and higher pH stability than the free lipase at pH 9.0. After six consecutive cycles, the residual activity of the immobilized lipase is still over 55% of its initial activity.
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RSC Advances
·
2014
·
IF 3.708
Vikramjeet Singh,Jinsong Zhu,Amita Nand,Zhiqiang Cheng,Yang Mo
Here we report the 3D photo-cross-linking strategy based on the use of α-cyclodextrin as an alternative surface chemistry for the preparation of small molecule microarray (SMMs). Surface plasmon resonance imaging (SPRi) was used in combination with SMMs as a detection technology. Small molecules of diverse structure containing different functional groups can be covalently captured on a single slide and displayed in various orientations in a given spot. Several known small molecules-protein complexes were selected to validate this platform. Furthermore, a comparison between 2D PEG and 3D α-cyclodextrin photo-cross-linking surface was performed using fluorescence and SPRi techniques. Data produced from both type of surfaces strongly favors the better performance of 3D over 2D surface in terms of loading capacity, spot morphology, signal response, and kinetics values. We believe that this strategy will expand the utility of small molecule microarray for high throughput screening against a variety of target proteins.
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RSC Advances
·
2015
·Vol. 5
·期 29
·23039-23045
·
IF 3.84
Weiwei Zhu,Yijing Li,Fang Zeng,Hang Yin,Liyuan Wang,Hao Zhu
Superparamagnetic Fe3O4 nanoparticles with diameter about 18 nm were prepared by a solvothermal method. A simple and green method was used to prepare amino-rich magnetic nanoparticles. The magnetic nanoparticles were modified by polyethylenimine (PEI) which is a polycationic polymer when pH < 10. The resulted magnetic nanoparticles were activated by glutaraldehyde to obtain nanoscale support for Candida rugosa Lipase (CRL) immobilization. The CRL was immobilized on the magnetic nanoparticles covalently, as well as via ionic exchange. The structure and magnetic behavior of the magnetic nanoparticles were confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), and vibrating sample magnetometer (VSM). Then the properties of the immobilized CRL were investigated, and the results showed that the obtained immobilized lipase displayed good reusability and applicability. The immobilized Candida rugosa Lipase (ICRL) presented wider pH tolerance (residual activity > 80% in a pH range from 5.5 to 8.0) than free Candida rugosa Lipase (residual activity decreased rapidly when the pH values were away from 7.0). ICRL showed excellent thermal stability (the relative of ICRL > 90%) after keeping in the water bath at 50 °C for 150 min while free Candida rugosa Lipase (FCRL) was absolutely deactivated. After being reused 10 times, ICRL maintained 60% relative activity.
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RSC Advances
·
2014
·Vol. 4
·期 71
·37470-37478
·
IF 3.708
Jianxiong Xu,Weiwei Liu,Yunfei Yu,Jingjing Du,Na Li,Lijian Xu
A simple synthesis of mesoporous silica nanoparticles with cubic Pmn pore structure (SBA-1) and controlled pore size is reported. The synthesis of mesoporous silica nanoparticles is achieved by employing organic mesomorphous complexes of polyelectrolyte (poly(acrylic acid)) and cationic surfactant (hexadecyl pyridinium chloride) as template, and tetrapropoxysilane as silica source. It is found that the pore size of the synthesized mesoporous silica nanoparticles can be tuned by the incorporation of 1,3,5-trimethylbenzene. By adjusting the amount of 1,3,5-trimethylbenzene in the synthesis, a series of silica nanoparticles with controlled pore size ranging from 2.5 nm to 5.3 nm is obtained. The adsorption properties of the mesoporous silica nanoparticles with different pore size against lysozyme are compared. The results show that mesoporous silica nanoparticles with proper pore size and well ordered pore structure benefit the adsorption and exhibit a low leaching rate.
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RSC Advances
·
2016
·Vol. 6
·期 89
·86455-86463
·
IF 3.289
Xue Qu,Feifei Wang,Yi Sun,Yu Tian,Rui Chen,Xiaoyu Ma,Changsheng Liu
Glycoproteins play important roles in various biological events. Extraction of specific bioactive glycoproteins from physiological fluids is highly required for clinical diagnosis and treatment. Concanavalin A (Con A) is a tetramer lectin protein that can specifically bind glycospecies containing sugar units, and this binding occurs in physiological environments. Inspired by this, we propose a sugar–lectin recognition based glycoprotein surface imprint, which is anticipated to selectively extract bioactive glycoproteins in physiological environments. The glycosylated biomarker, transferrin, was used as a model template. Transferrin was first immobilized on a Con A modified Fe3O4 surface via the sugar–lectin interaction at pH 7.4. Dopamine was then polymerized onto this surface for glycoprotein imprinting at the same pH, and continuous oxygen bubbling into the dopamine solution improved the polymerization speed. After removal of the template by boronate buffer, transferrin imprinted Fe3O4 was obtained. The subsequent binding experiment indicated that the transferrin imprint has pH dependent binding affinity towards this protein. The optimal transferrin binding capacity, as well as the maximum imprinting factor was observed at neutral pH. The isotherm adsorption study reveals that imprinted and non-imprinted material are both fitted with the Langmuir adsorption model. Single selective adsorption and competitive adsorption experiments show that the obtained surface imprint has selectivity towards template glycoprotein, and circular dichroism (CD) spectral analysis indicates that boronate buffer washing has no negative influence on the natural structure of transferrin and Con A. These results demonstrate that this novel glycoprotein surface imprint can work under physiological conditions, and the glycoprotein extraction method is mild enough to keep the bioactivity of these targets for further bio-application.
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NUCLEAR ENGINEERING AND DESIGN
·
2024
·
IF 1.9
Shiyin Ji,Xiuhua Ji,Yanhao Song,Yanxia Wei,Tao Duan
Regulation of microstructure and processing cost of waste form are important for the design and application of high-level radioactive waste immobilization. In this study, CaZrTi 2 O 7 (zirconolite) – CaNdTiNbO 7 (pyrochlore) ceramics were synthesized via conventional sintering method at 1000–1500 °C to investigate the effect of sintering temperature on the microstructure, solidification, and crystal evolution. The powder x-ray diffraction (XRD) and backscattered scanning electron microscopy–energy dispersive spectroscopy (BSEM–EDS) results indicated gradual formation of zirconolite and pyrochlore ceramics, following with their densification when increasing sintering temperature. It was found that sintering temperature should be higher than 1300 °C to form zirconolite-pyrochlore solid solutions. Densified pyrochlore was obtained at 1500 °C. In addition, transformation from relatively low-symmetry lattice to higher-symmetry lattice was found with an increase in sintering temperature. Furthermore, higher temperature can enhance the crystal growth and densification in the ceramic composite. The finding in this study could provide important suggestions to design and process zirconolite-pyrochlore waste forms for industrial application.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Huachuan Feng,Yilin Wang,Nan Li,Zishu Qian,Tao Chen,Xiaoming Chen,Qing Wang,Wenkun Zhu
Uranium-stressed soil caused by nuclear industry development and energy acquisition have attracted extensive attentions for a long time. This study investigated the effects of biochar application with different pyrolysis temperatures (300 ℃, 500 ℃ and 700 ℃) on remediation of uranium-stressed soil. The results showed that higher pyrolysis temperature (700 ℃) was benefit for ryegrass growing and caused a lower uranium accumulation in plants. At the same time, uranium immobilization was more effective at higher biochar pyrolysis temperature. Careful investigations indicated that activities of soil urease and sucrase were promoted, and bacterial diversity was strengthened in C700 group, which may contribute to uranium immobilization. The biochar application could activate metabolic of lipids and amino acids , organic acids and derivatives, and organic oxygen compounds. Nicotinate and nicotinamide metabolism, and Benzoxazinoid biosynthesis were unique metabolic pathways in the C700 group, which could enhance the uranium tolerance from different perspectives. Based on these results, we recommend to use biochar with 700 °C pyrolysis temperature when processing remediation of uranium-stressed soil. This study will facilitate the implementation of biochar screening and provide theoretical helps for remediation of uranium-stressed soil.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-31
·
IF 3.9
Tian Kun,Li Chunping,Liu Huiming,Wang Lianchun
To enhance the adsorption capacity of biochar (BC), herein a novel multifunction modified biochar (SDMBC) was prepared by directly crosslinking of the nanomicelle of sodium dodecyl sulfate/sapindus-saponin (SDS/SAP) composite system onto the BC through a simple, environmental friendly approach. Result showed that the adsorption performance of SDMBC has been greatly improved, compared with BC or using alone SDS and SAP, adsorption ability increased by 48.83%, 29.50%, 36.44%, respectively, the best modified effect was appeared when the concentration of SAP to SDS was 0.8 and 0.8 CMC. SDMBC exhibited high adsorption abilities of 130.23, 108.43, 277.09 125.27, 112.78 mg/g for heavy metal ions lead Pb(II), Cadmium Cd(II) and organic pollutants with different chemical properties bisphenol A(BPA), Methylene blue (MB), P-nitrophenol (PNP), respectively, higher than most previously reported adsorbents, importantly, SDMBC can still efficient removal capabilities even in the binary competition. Subsequently, the SDMBC and BC was characterized by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), Zeta potential (Zeta), it found SDMBC has a more layered structure, richer functional groups and more amorphous structure compared with BC, which are closely related with improving its adsorption capacity. The adsorption behavior of SDMBC for MB show that process was found to be spontaneous, propitious, endothermic, the adsorption isotherms fitted Freundlich models well, pseudo-second-order best describes kinetics adsorption, suggesting that the process is multi-layer chemical adsorption. The little affected by ionic strength and coexisting substances, could remained removal rate over a wide pH range, SDMBC still keep high removal rates even after 5 reuses. Based on FT-IR analysis, plausible adsorption mechanism proposed, including hydrogen bond, electrostatic attraction and π-π bonding. Cost analysis manifests that the SDMBC are high efficiency and cheap eco-adsorbents compared with commercial activated carbon, and the SDMBC dosage required for the removal of 99% of a fixed amount of MB in different volumes of effluent was predicted. Seven machine learning (ML) models were used to predict the MB (60 mg/L) adsorption of the SDMBC, using Shapley Additive Explanations (SHAP) for model interpretation. Finding Extreme Gradient Boosting (XGBoost) exhibited best performance, the order of feature importance as time> Ratio> pH> concentration> temperature. Thus, SDMBC as a new cheap and eco-adsorbents, can be used to effectively remove various types of pollutants, has a great application potential in sewage treatment, while the accurate ML prediction model presented a valuable advice for designing efficient adsorbents and optimization operating conditions in the future.
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