MATERIALS SCIENCE FORUM
·
2019
·
IF 0
Zhi Min Ou,Jia Ying Pan
In this study, magnetic chitosan microspheres (Fe3O4-CTS) were prepared via chemical co-precipitation and cross-linked with lipase using glutaraldehyde to form Fe3O4-CTS-glutaraldehyde-lipase particles. The textural characteristics of Fe3O4-CTS-glutaraldehyde-lipase particles were assessed by scanning electron microscopy. The optimal immobilization conditions were 2.1 mg/mL lipase, 10 mg/mL Fe3O4-CTS-glutaraldehyde, pH 7.5, 30 °C, 2 h. The loading amount of lipase was 126.0 mg/g carrier, and the specific activity reached to 46.7 U/mg. Fe3O4-CTS-glutaraldehyde-lipase particles was used in resolution of racemic 1-phenylethylamine in a solvent-free system. The conversion and enantiomeric excess of (R)-N-(1-phenylethyl)acetamide reached 33.6% and 97%.
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BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Reziwanguli Wusiman,Shayila Haimiti,Hanikezi Abuduaini,Miaoyan Yang,Yitian Wang,Meijun Gu,Ali Sailike,Lei Gao
Renal fibrosis is a prominent feature of diabetic nephropathy (DN), and the connection between renal fibrosis and abnormal glycolysis is not fully understood. SUMO-activating enzyme subunit 1 (SAE1) plays a crucial role in the SUMO modification process and is related to abnormal glycolysis. Despite this, the specific role of SAE1 in DN and its mechanism are not well defined. To investigate this, a streptozotocin-induced diabetic CD1 mice model was used, with SAE1 suppression achieved through systemic administration of SAE1 siRNA. In parallel, human renal proximal tubular tubule HK2 cells transfected with siSAE1 were exposed to high glucose for in vitro studies. The study revealed that SAE1 levels were elevated in diabetic kidney, and the deletion of SAE1 mitigated renal fibrosis in DN mice. Such suppression in SAE1 was associated with the lower expression of hypoxia inducible factor-1α (HIF-1α) and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), these alterations subsequently improved abnormal glycolysis and mesenchymal transformations in vivo and in vitro . Further experiments discovered that SAE1 stabilized transcription factor HIF-1α expression through SUMOylation, promoting PFKFB3 transcription, which enhanced glycolysis characterized by increased PFK1 activity and lactate production. Additionally, pharmacological inhibition of PFKFB3 reduced renal fibrosis in DN mice, while overexpression of PFKFB3 partly restored the glycolysis and mesenchymal transformations inhibited by SAE1 knockdown in vitro . These data demonstrate that SAE1 promotes abnormal glycolysis by HIF-1α/PFKFB3 which is responsible for the fibrotic phenotype of diabetic kidney. Inhibition of SAE1 could be an alternative strategy in combating diabetes associated-kidney fibrosis via improving aberrant glycolysis.
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PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Heng Tang,Ke Ning,Boji Wu,Xuhong Wang,Jingyu He,Pingping Li,Lina Pan,Jiawen Zhang,Yi He,Shizhu Bian,Xingyu Ma,Jihang Zhang,Chuan Liu,Zhexue Qin,Houyuan Hu
Scutellarein (Sc), a natural flavonoid, holds potential for treating pulmonary arterial hypertension (PAH), yet its mechanisms remain unexplored. This study investigated Sc’s therapeutic effects and underlying pathways in PAH. In vivo experiments demonstrated that Sc significantly attenuated right ventricular hypertension, pulmonary arterial remodeling, αSMA expression, and vascular inflammation in PAH models. In vitro, Sc suppressed hypoxia-induced proliferation, migration, inflammation, and pyroptosis in human pulmonary artery smooth muscle cells (HPASMCs). Mechanistically, Sc activated the SIRT1/NAD + axis to restore mitochondrial homeostasis: it upregulated SIRT1 expression and elevated NAD + levels by promoting SIRT1-mediated deacetylation of nicotinamide nucleotide transhydrogenase (NNT), thereby enhancing NNT activity. Elevated NAD + further activated SIRT1, forming a self-reinforcing SIRT1/NNT/NAD + feedback loop that mitigated hypoxia-induced mitochondrial dysfunction. This study identifies Sc as a novel regulator of the SIRT1-dependent NNT deacetylation pathway, which stabilizes NAD + homeostasis to counteract HPASMCs dysregulation in PAH. These findings highlight Sc’s potential as a therapeutic candidate for PAH, offering insights into targeting mitochondrial-metabolic pathways for vascular remodeling diseases.
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PubMed
高影响因子
ANALYST
·
2015
·Vol. 140
·期 13
·4662-4667
·
IF 4.107
Yaqi Hu,Chunyan Du,Yunchao Li,Louzhen Fan,Xiaohong Li
Direct and rapid detection of 1-hydroxypyrene (1-OHP) is of great importance owing to its high carcinogenicity, teratogenicity and toxicity. In this study, a simple colorimetric assay for rapid determination of 1-OHP is reported, which is based on non-crosslinking aggregation of gold nanoparticles (Au NPs) induced by 1-OHP in the presence of formic acid (FA). Initially, Au NPs were synthesized with citrate as the capping agent and exhibited red color. Subsequently, the addition of FA did not cause aggregation of Au NPs, but a proton transfer process occurred from FA to carboxylic anions on the surface of Au NPs with a decreased zeta potential. The subsequent addition of 1-OHP resulted in a further decreased zeta potential and an intensely hydrophobic environment, which led to a strong and rapid non-crosslinking aggregation of Au NPs within 5 min with the color changing from red to violet blue. Based on this principle, sensitive and selective detection of 1-OHP was achieved. The detection limit was 3.3 nM. Finally, the colorimetric assay was successfully applied to detect 1-OHP in a urine sample. This strategy provides new insights into developing colorimetric methods for on-site and real-time detection of polycyclic aromatic hydrocarbons.
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高影响因子
IEEE SENSORS JOURNAL
·
2025
·
IF 4.5
Ziheng Zhi,Fangrong Hu,An Su,Mingzhu Jiang,Wenying Jiang,Longhui Zhang,Zhongpeng Gao,Weiyu Luo,Chengju Meng
P-Xylene is a prevalent indoor hazardous volatile organic compound(VOC). A long-term exposure limit of 100 ppm for p-xylene is generally recommended. Therefore, sensitive and selective detection of trace p-xylene is crucial. This work presents a method for detecting p-xylene gas by combining terahertz (THz) metasurface sensor with a metal-organic-framework (MOF) material, copper benzene-1,3,5-tricarboxylate (HKUST-1). The sensor with double split ring resonators is fabricated using a micromachining process and characterized by a terahertz time-domain-spectroscopy (TDS) system. The test results are consistent with the simulation. Subsequently, the sensor's surface is modified using the MOF material of HKUST-1 to enhance its sensitivity to the p-xylene gas. At room temperature, the sensitivity for p-xylene gas concentrations is between 1-100 ppm .The modified sensor has a maximum response frequency shift of 14 GHz for p-xylene gas at 100 ppm. Furthermore, the specificity, repeatability and stability of the sensor are characterized. The proposed method for highly sensitive and specific detection of p-xylene gas has important reference significance for the development of other types of gas sensors.
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JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2024
·
IF 2.7
Chunying Du,Hua Yin,Aowei Xie,Junhong Yu,Yifan Wang,Fengli Yao,Siyu Zhang,Yidan Zhang,Lu Liu,Peng Wang,Jianjun Dong,Ximing Xu
Hyperuricemia is mainly caused by insufficient renal urate excretion. Urate transporter 1 (URAT1), an organic anion transporter, is the main protein responsible for urate reabsorption. In this study, we utilized artificial intelligence-based AlphaFold2 program to construct URAT1 structural model. After molecular docking and conformational evaluation, four e-pharmacophoric models were constructed based on the complex structures of probenecid-URAT1, benzbromarone-URAT1, lesinurad-URAT1, and verinurad-URAT1. Combining pharmacophore modeling, molecular docking, MM/GBSA calculation and ADME prediction, 25 flavonoids were selected from the natural products database containing 10,968 molecules. Then, a model of HEK-293T cells overexpressing URAT1 was constructed, and the inhibitory activity to URAT1 of 25 flavonoids was evaluated by measuring their effect on cellular uptake of 6-carboxyfluorescein (6-CFL). Fisetin, baicalein, and acacetin showed the best activity with IC50 values of 12.77, 26.71, and 57.30 µM, respectively. Finally, the structure-activity relationship of these three flavonoids was analyzed by molecular docking and molecular dynamics simulations. The results showed that the carbonyl group on C-4 and hydroxyl group on C-7, C-4', and C-5’ in flavonoids were conducive for URAT1 inhibitory effects. This study facilitates the application of flavonoids in the development of URAT1 inhibitors.Communicated by Ramaswamy H. Sarma
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高影响因子
Bioengineered
·
2022
·
IF 6.832
Yazhou Sun,Weiguo Wan,Xin Zhao,Xueyu Han,Tianxin Ye,Xiaoli Chen,Qian Ran,Xiukun Wang,Xin Liu,Chuan Qu,Shaobo Shi,Cui Zhang,Bo Yang
Sigma 1 receptor (S1R) has shown a preferable protective effect on left ventricular function, but whether it protects right ventricular (RV) function is still elusive.This study aimed to determine the effects of S1R on RV dysfunction secondary to pulmonary arterial hypertension.Sixty wild-type male Sprague–Dawley rats were randomly divided into the control group, the fluvoxamine group, the pulmonary arterial hypertension group and the pulmonary arterial hypertension combined with fluvoxamine group. Monocrotaline (60 mg/kg) was administered to induce pulmonary arterial hypertension, and fluvoxamine was given for 21 consecutive days to activate S1R after one week of monocrotaline administration. Echocardiographic, serologic, and histologic parameters, qRT-PCR, and western blotting were conducted after 4 weeks of monocrotaline administration.The expression of S1R was decreased in the right ventricle in pulmonary arterial hypertension. TAPSE, and the FAC of the right ventricle were significantly decreased, and RV EDP and the plasma concentration of N-terminal pro-B-type natriuretic peptide was increased in the pulmonary arterial hypertension group, but fluvoxamine partly restored those abnormalities (all P < 0.05). Moreover, pulmonary arteriole remodeling, and fibrosis and hypertrophy in the RV were shown in the pulmonary arterial hypertension group; interestingly, fluvoxamine recovered RV structural remodeling (all P < 0.05) but neither alleviated pulmonary arteriole remodeling nor reduced pulmonary artery pressure. Furthermore, S1R activation protects RV function by upgrading the NRF 2/HO 1-mediated antioxidant stress pathway. In conclusion, chronic S1R activation ameliorates structural remodeling and RV dysfunction secondary to pulmonary arterial hypertension without altering pulmonary artery pressure.
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PubMed
高影响因子
LIQUID CRYSTALS
·
2020
·
IF 2.908
Jiazhen Dang,Shenghua Du,Liyuan Ren,Mengting Zhang,Pei Chen,Aiai Gao,Weisong Du,Xinbing Chen,Zhongwei An
Heterocyclic mesogenic compounds are one of the interesting liquid crystals. Here, a series of 1-methyl-1H- benzimidazole-based mesogenic compounds incorporating ethynyl moiety, 2-[4-[2-[4-(alkoxy)phenyl]ethynyl]phenyl]-1-methyl-1H-1,3-benzimidazole derivatives (nPEPMx) with terminal groups of H, CH3 and NO2 (coded as nPEPMH, nPEPMM and nPEPMN, respectively), are synthesised and their properties are evaluated. It is noted that the compounds nPEPMx with short alkoxy chain less than 9 carbon atoms display enantiotropic nematic mesophases, whereas corresponding reference compounds without ethynyl moiety have enantiotropic smectic mesophases. The mesophase ranges of compounds nPEPMx 50–70°C and 55–108°C on heating and cooling for nPEPMH, 76–106°C and 98–142°C for nPEPMM, and 78–132°C and 130–166°C for nPEPMN, respectively. Besides, compounds nPEPMx show obviously large birefringence of 0.501–0.641, suggesting a potential application in liquid crystal mixture. The high birefringence may result from its large π-conjugated molecule structure incorporating ethynyl and benzimidazole π-conjugated mesogenic unit.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Fangyi Pei,Zhimiao Wang,Jianhua Lv,Fang Li,Wei Xue
Cu–Zn–Al catalyst was prepared by the co-precipitation method, and the catalytic performance for transfer hydrogenation of acetophenone (AP) to 1-phenylethanol (1-PhE) was evaluated using isopropanol (IPA) as a hydrogen source. The addition of Zn changed the morphology of the catalyst and reduced aggregation of catalyst particles, resulting in highly dispersed Cu active sites. As the Zn content increased, the valence state of Cu species changed. When the Cu/Zn molar ratio was 2/3, the Cu+/Cu0 ratio in the catalyst was 0.81, resulting in good selectivity for 1-PhE. The reaction conditions were optimized over the Cu–Zn–Al catalyst with a Cu/Zn molar ratio of 2/3. When the reaction was performed at 180 °C for 2 h with an IPA/AP molar ratio of 15, the AP conversion was 89.4% and the 1-PhE selectivity was 93.2%. The Cu–Zn–Al catalyst showed good stability, and AP conversion and the 1-PhE selectivity were essentially unchanged after five cycles.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 16
·13496-13502
·
IF 2.22
Fang Weishuang,Ao Laiyuan,Tang Ying,Li Jie,Xiang Huaicheng,Zhang Zhiwei,Du Qianbiao,Fang Liang
Recently, there have been considerable interests in the Li-containing spinel oxides as promising microwave dielectric materials. Li loss at elevated temperatures, however, is known to be inevitable, resulting in deterioration on dielectric performances. Here the influence of Li nonstoichiometry on the microwave dielectric properties of Li 2 ZnGe 3 O 8 , a spinel oxide, was reported. An appropriate level of Li excess enhanced the densification of Li 2 ZnGe 3 O 8 and increased dielectric performances. In contrast, high level of Li excess induced the formation of the secondary phase of Li 2 ZnGeO 4 confirmed by XRD and Raman spectra, which in turn lowered the dielectric properties. Typically, a composition with x = 0.075, in the Li 2(1+ x ) ZnGe 3 O 8 ceramics exhibited the enhanced microwave dielectric properties with ε r = 10.68, Q × f = 77,300 GHz (at 13.3 GHz), and τ f = − 70.35 ppm/°C when sintered at 940 °C for 4 h. Moreover, these compounds have well chemical compatibility with the silver electrodes.
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ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xingguo Zhou,Hanghang Liu,Yanhui Zheng,Yaobao Han,Tingting Wang,Hao Zhang,Qiao Sun,Zhen Li
Radiotherapy has been extensively used to treat cancer patients because it can effectively damage most solid tumors without penetration limits. A hypoxic microenvironment in solid tumors leads to severe radioresistance and expression of hypoxic inducible factor-1 (HIF-1), which results in poor efficacy of radiotherapy alone. Herein, we report the excellent efficacy of radiotherapy achieved using a new type of yolk–shell Cu2–xSe@PtSe (CSP) nanosensitizer functionalized with the HIF-1α inhibitor acriflavine (ACF). We prepare the CSP nanosensitizer through the interfacial redox reactions between chloroplatinic acid and Cu2–xSe nanoparticles (CS) and then functionalize the nanosensitizer with ACF through their electrostatic interactions. We show that the synthesized CSP nanosensitizer can arrest the cell cycle (i.e., at the gap 2/mitosis (G2/M) phases) of tumor cells to enhance their sensitivity to X-rays and decompose endogenous H2O2 into O2 to reduce hypoxia and increase the production of reactive oxygen species, which leads to severe damage to DNA double strands and apoptosis of tumor cells. We also show that the ACF on the surface of CSP nanoparticles can effectively reduce the expression of HIF-1α. All these effects lead to a low vascular endothelial growth factor, low density of microvessels in tumor, decreased cell proliferation, and increased cell apoptosis, which synergistically and drastically enhance the efficacy of radiotherapy. This work provides insights and guidance for developing novel nanosensitizers to enhance the efficacy of radiotherapy.
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高影响因子
Journal of CO2 Utilization
·
2017
·
IF 4.292
Adriano Zanon,Somboon Chaemchuen,Bibimaryam Mousavi,Francis Verpoort
A newly Zn-doped ZIF-67 has been synthesized in water at room temperature for the first time without the aid of any organic solvent. The textural and physical properties of the material were analyzed by X-ray diffraction (XRD), thermogravimetric analysis (TGA), N 2 adsorption-desorption, scanning electron microscopy (SEM) and ICP-AES. The catalyst showed quantitative conversions and selectivity for the synthesis of cyclic carbonates starting from epoxides and carbon dioxide. Compared with different reported MOFs as heterogeneous catalysts used for the cycloaddition of carbon dioxide to epoxides, this catalyst outperforms all the reported ones till now. The coupling reaction was studied at different pressures and temperatures applying different epoxide substrates. Additionally, no co-catalyst and solvent were required to obtain excellent conversions. The heterogeneous nature of the catalyst was maintained at least up to four catalytic cycles with a slight loss of activity.
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