CHEMOSPHERE
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2019
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IF 5.108
Jiajun Long,Longqian Xu,Linghui Zhao,Huaqiang Chu,Yunfeng Mao,Deli Wu
In this study, Cu(0) was synthesized with NaBH 4 as a reducing agent for Cu(II) and used to activate dissolved molecular oxygen (O 2 ) under acidic conditions. The Cu(0) synthesized had much higher activity than the purchased. The roles of Cu was clarified and the formation of reactive oxygen species was discussed through direct detection for the first time. By detecting the valence change of Cu in a CuO system, Bisphenol A (BPA) was found to accelerate the transformation of Cu(II) to Cu(I). Besides, the evidence from electron spin resonance (ESR) studies and scavenging tests revealed the new roles of Cu(0) that Cu(0) could not only convert O 2 to produce ·O 2 − , but also catalyze H 2 O 2 to ·OH. The results from this study offer evidence of new reaction pathways in Cu-activated O 2 systems and deepen understanding of the reaction between Cu species and O 2 .
研究领域:
环境科学
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化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 46
·28637-28646
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IF 3.108
Pei Song,Lisha Zhang,Hao Long,Meng Meng,Ting Liu,Yongmei Yin,Rimo Xi
In this research, we developed a multianalyte fluorescence sensing system through a carbon dots (CDs)-based fluorescent probe that can specifically recognize Fe(III) by fluorescence quenching. The CDs prepared using black tea by a hydrothermal method show outstanding properties like low cytotoxicity, high photostability, excellent biocompatibility, and high sensitivity. It was found that the fluorescence of CDs can be quenched by micromolar concentrations of Fe(III) in both aqueous solutions as well as living cells. It is well known that glucose can be oxidized by glucose oxidase (GOx) to release H2O2, which, in turn, can oxidize Fe(II) to Fe(III). Based on this consideration, a multianalyte sensing system was established. Therefore the quantitative analysis of Fe(III), H2O2, and glucose with detection limits of 0.25 μM, 0.82 μM, and 1.71 μM, respectively, was achieved by the simple and cost-effective multianalyte CDs sensing system constructed. The sensing system showed high photostability and negligible cytotoxicity toward HeLa cells, which enables it to be applied in the visualization of Fe(III) or H2O2 in living cells. The system was further applied in the detection of Fe(III) or glucose in human serum, and satisfactory results were obtained.
研究领域:
分析化学
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材料科学
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生物化学
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生物医学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Mei Liu,Jinxin Zhang,Shasha Liu,Baoxin Li
ZEN-responsive hydrogel was prepared using ZEN aptamer and a cationic conjugated skeleton which was formed by the electrostatic interaction between acrylamide and poly (diallyldimethylammonium chloride) (PDDA). In the presence of ZEN, the binding of aptamer and ZEN caused the hydrogel to dissociate, releasing the gold nanoparticles (AuNPs) encapsulated in the hydrogel into supernatant, whose color changes serving as response instructions were observed by eyes. To realize low dose visual detection, the TMB-H 2 O 2 was used for quantitative readout by AuNPs released from the hydrogel which can efficiently catalyze the reaction to generate a obvious change.There was a good linear relationship between the changed absorbance and concentration of ZEN within 2.5–100 ng/mL, a detection limit of 0.98 ng/mL, recovery rates of 98.8%-101.3% and 99.8%-101.5% for corns and beer, respectively. This developed sensing strategy would provide a promising application for other mycotoxins by replacing the corresponding aptamer sequences.
研究领域:
分析化学
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纳米技术
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食品安全
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毒理学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ziqi Ye,Changshuai Shang,Yang Wang,Zhifan Liu,Chaihong Gong,Xiaoxiao Ge,Weiying Zhang,Jing Li,Zhenzhong Guo
Utilizing the peroxidase (POD) activity of nanozymes to construct the colorimetric sensors is a popular strategy for assessing the antioxidant capacity of biological molecules, but the detection sensitivity is often compromised by their low enzymatic activity. Pt-based nanozymes, favored by their regulated nanostructures, well provide numerous binding sites for H 2 O 2 , and exhibit enhanced POD performance. Here, we utilized a simple hydrothermal method to synthesize PtTeNi nanomaterials featured with the ultrathin sheets for detecting biological antioxidant (ascorbic acid (AA) as the model). With the TMB-H 2 O 2 system as the colorimetric substrate, PtTeNi nanomaterial exhibited superior POD-like activity with a higher Vmax (5.02 × 10 −4 mM s −1 ) and lower Km (3.14 mM)) in contrast to natural HRP. AA significantly inhibited the colorimetric reaction of PtTeNi-TMB-H 2 O 2 system via competitively binding OH generated by PtTeNi-mediated H 2 O 2 catalysis and further blocking oxTMB formation. Inspired by this fact, the PtTeNi-TMB-H 2 O 2 colorimetric sensor successfully realized highly sensitive and selective detection of AA with a good linear range from 5 μM to 500 μM and the detection limit of 2.94 μM. For analyzing the total antioxidant capability and AA contents in actual samples (commercial drinks and vitamin C tablets), the PtTeNi-based colormetric method displayed comparable results with the commercial total antioxidant capacity assay kit, with the advantages of easy operation, convenient storage and high stability. This work fully affirms the excellent enzyme-like performance of Pt-based nanomaterial and confirms the feasibility of nanozymes-based colorimetric sensing strategy in detecting antioxidant capability of biological molecules.
研究领域:
纳米技术
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分析化学
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生物传感器
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材料科学
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产品关联
DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Kaiwei Wang,Hao Ruan,Fumin Wang,Yi Zhai,Mingshuai Sun,Yan Chen,Jiaxing Zhang,Yihao Wang,Xuyang Zou,Xubin Zhang
The construction of abundant TiO 6 active sites within the zeolite framework is essential for further enhancing the catalytic performance of TS-1 zeolite. Here, we employ a strategy involving treatment with a mixture of amines and ammonium salts to generate abundant TiO 6 species within zeolites synthesized with the assistance of N,N-dimethylformamide (DMF). It was observed that during the post-treatment process, the presence of amines and ammonium salts plays a key role for the formation of highly active TiO 6 species. The incorporation of DMF can significantly enhance the TiO 6 content in the post-treated zeolite, increasing it to 61.5 % compared with 28.5 % in the zeolite synthesized without DMF. The effect of the DMF/SiO 2 ratio in the synthesized gel on the morphology and catalytic performance was also examined. It is noteworthy that the incorporation of DMF influences the microenvironment of the Ti active sites following post-treatment. 1 H MAS NMR analysis reveals that the characteristic peaks corresponding to Ti-OH and isolated Si-OH groups in the DMF-synthesized catalyst are significantly intensified after treatment with amines and ammonium salts. When H 2 O 2 is employed as an oxidant, the optimal catalytic performance achieves a cyclohexene conversion of 51.1 % and a cyclohexene oxide yield of 39.4 %, with a high TOF value of 92.77 h −1 . Notably, the catalyst exhibits a significantly conversion rate of 79.4 % and a yield of 72.7 % using O 2 as the oxidant.
研究领域:
催化化学
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材料科学
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无机化学
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石油化工
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绿色氧化
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产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2020
·Vol. 8
·期 43
·22720-22727
·
IF 11.301
Xueming Dang,Ruying Yang,Zhe Wang,Siyi Wu,Huimin Zhao
Graphitic carbon nitride (g-C3N4) possesses unique electronic and optical properties in the visible-light-driven production of H2O2. However, bulk g-C3N4 suffers from inefficient sunlight absorption and low carrier mobility. Herein, P doped g-C3N4 with a hollow sphere architecture was synthesized. The hollow sphere structure provides a large accessible surface area for charge transfer and enhances photo-absorption properties. Particularly, the doped P can regulate the energy band structure with stronger reducibility for O2 activation and accelerate photo-generated carrier separation. Meanwhile, the P heteroatom is beneficial to produce 1,4-endoperoxide species more easily and enhance selectivity for H2O2 synthesis through a two-electron O2 reduction process. The resultant catalyst exhibits a high H2O2 production rate of 174 μmol h−1 g−1 in pure water and 1684 μmol h−1 g−1 with isopropanol as a hole scavenger, which are 7.5 and 11.2 times those of bulk g-C3N4. This work provides a new avenue for the design and modification of other photocatalysts for H2O2 production.
研究领域:
材料科学
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光化学
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催化
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ling Zhang,Shuhan Yin,Jiaze Hou,Wenping Zhang,Hui Huang,Yongxin Li,Cong Yu
Choline is an essential nutrient for the growth and development of the baby, and therefore it is often added to infant formula. In this paper, a novel sensor for choline determination in infant formula is developed based on upconverting nanoparticles (UCNPs) with near infrared luminescence. UCNPs-based detection can avoid the interference of background fluorescence from complex samples, and thus provide high selectivity and sensitivity. It was observed that in the presence of Fe 3+ , polyacrylic acid coated UCNPs were quenched to 3% of its original intensity. The degree of quenching was among the best for UCNPs. Hydrogen peroxide could oxidize Fe 2+ to Fe 3+ , which caused quenching of the upconversion luminescence. A new H 2 O 2 detection method was thus established. In addition, choline could be hydrolyzed to betaine by choline oxidase, and at the same time produced H 2 O 2 , which also caused luminescence quenching through Fe 2+ oxidation. Therefore, selective choline sensing was achieved.
研究领域:
分析化学
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纳米技术
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食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2024
·
IF 6.1
Xuwen Da,Yunli Xu,Lei Wang,Xiulian Liu,Yatong Peng,Yao Wu,Wanpeng Zhou,Wentao Wang,Xuesong Wang,Qian-Xiong Zhou
Traditional photodynamic therapy (PDT) is almost ineffective against deeply seated tumors due to the limited penetration depth of external light. The development of chemiexcited PDT is promising to address this issue, but it is still challenging to construct simple, efficient and targeted chemiexcited PDT systems. In this work, CPPO (bio(3,5,6-trichloro-2-n-pentyloxycarbonylphenyl) oxalate) and [Ru(phen)2(PIP-OCH3)](PF6)2 (Ru3, where phen = 1,10-phenanthroline, PIP-OCH3 = 2-(4-methoxy phenyl)-1Himidazo[4,5-f][1,10]phenanthroline) with efficient photo-catalytic NADH oxidation were co-encapsulated with DSPE-PEG 2000 to form Ru3/CPPO@PEG nanoparticles (NP3). Once internalized by cancer cells, Ru3 can be excited by the chemical energy produced by the reaction between CPPO and endogenous H2O2. The subsequent oxidation of intracellular NADH and generation of H2O2 in situ by excited Ru3 will further promote the sustained occurrence of chemical reaction, realizing a self-enhanced chemiexcited PDT. NP3 could effectively deplete intracellular NADH, inhibit ATP synthesis, increase H2O2 content, and produce reactive oxygen species (ROS) within cancer cells, thus exhibited efficient chemiexcited PDT activity against various cancer cells. Notably, minimal cytotoxicity was observed towards normal cells. Good therapeutic effects against subcutaneously implanted and lung metastatic tumors were also observed for NP3. The current findings may provide new sights for development of novel chemiexcited PDT.
研究领域:
光动力疗法
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癌症研究
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纳米技术
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生物化学
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药学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Wenyuan Zhao,Ying Ma,Jianshan Ye,Jiye Jin
Many quantum dots (QDs) are considered as excellent electrochemiluminescence (ECL) fluorophores with high quantum yields (QYs) and good stability. However, their ECL efficiency dramatically decreases in the aqueous solution compared to that in organic phase, which significantly limits their applications in bioanalysis. In this study, we developed a new sensing strategy via the combination of a closed bipolar electrode and electrochemiluminescence (CBP-ECL) system, which allows the separation of sensing in aqueous media and generation of ECL in organic media. Two kinds of QDs, CdSe/ZnS and CsPbBr 3 were applied as ECL fluorophores in this system, and stable, high QYs ECL signals were achieved. The resulting sensing platform demonstrated high sensitivity for the detection of H 2 O 2 with a linear range of 0.02−12 mM and the limit of detection (LOD) of 9.2 × 10 −8 M when CdSe/ZnS QDs were used as ECL reagents. We envision that this system can be widely employed in any kind of bioanalysis which can produce the electrochemical currents in the detecting cell. More importantly, we expect this system could break through the limitation of the fluorophores, especially those with solvent dependent ECL signals such as aggregation-induced emission (AIE) molecules, or most of the hydrophobic dyes.
研究领域:
分析化学
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生物化学
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纳米技术
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电化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-7
·
IF 6.232
Huang Qingwei,Zhang Jinli,Li Wei,Fu Yan
Heparin was employed as the stabilizing agent in the synthesis of peroxidase-mimicking Pd nanoparticles. The heparin-capped Pd nanozyme can act as both the signal amplifier and the selective binder of protamine. The most efficient nanozyme with the mean size of 3.5 nm consists of 70.8% metallic Pd 0 and 29.2% Pd 2+ species. Enzyme kinetic studies show that the K m values are 0.036 mM for 3,3′,5,5′-tetramethylbenzidine and 78 mM for H 2 O 2 . Protamine shows strong affinity to the heparin-capped Pd nanozyme, and induces an apparent aggregation of the nanoparticles. This results in a significant inhibition of the peroxidase-mimicking activities. Hence, the oxidation of TMB by H 2 O 2 to a blue product with a maximum absorption at 652 nm is suppressed. Based on this finding, a photometric assay is developed for the determination of protamine. The linear response is in the concentration range 0.02 ~ 0.8 μg mL −1 , and the limit of detection is 0.014 μg mL −1 . This assay presents high selectivity toward other biological substances.
Highly active and selective Pd nanozyme was synthesized through adopting heparin as the capping agent for quantitative determination of protamine.
研究领域:
分析化学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Mei-Yuan Shi,Ming Xu,Zhi-Yuan Gu
Ultrathin two-dimensional (2D) metal-organic framework (MOF) nanosheets have attracted numerous attention due to their unparalleled advantages such as unique structures, large surface areas, good stability and high catalytic efficiency . In this paper, 2D Cu(bpy) 2 (OTf) 2 nanosheets (Cu-MOF, bpy = 4,4-bipyridine, OTf = trifluoromethanesulfonate) are first utilized to achieve the fluorescent detection for H 2 O 2 and glucose with their intrinsic peroxidase-like activity. The fluorescent biosensor Cu-MOF nanosheets were achieved by the efficient catalysis of non-fluorescent thiamine (TH) to strong fluorescent thiochrome in the presence of H 2 O 2 . With the combination of glucose oxidase , highly selective and sensitive fluorescent detection for glucose could be established with a detection limit of 0.41 μM and two separate linear ranges of 10–100 μM and 100–1000 μM, respectively. Furthermore, the developed method has been applied to human serum for the quantitative determination of glucose as a clinical diagnosis indicator of diabetes mellitus.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Shuang Zhou,Da Wen,Wei Zhong,Jianjun Zhang,Yaorong Su,Aiyun Meng
The effective adsorption of oxygen (O 2 ) molecules over photocatalysts is a critical step in promoting the performance of photocatalytic H 2 O 2 production. However, g-C 3 N 4 usually features a Yeager-type (side-on) adsorption configuration of O 2 molecules, which causes the breaking of O–O bonds and severely hinders the H 2 O 2 production activity. Herein, we synthesized an oxygen-vacancy-rich TiO 2– x /g-C 3 N 4 step-scheme (S-scheme) heterojunction to regulate the oxygen adsorption configuration and improve the 2e – ORR selectivity of H 2 O 2 production. In-situ X-ray photoelectron spectroscopy (in-situ XPS) and density functional theory (DFT) calculations reveal that the S-scheme heterojunction is formed between TiO 2– x and g-C 3 N 4 . The difference between their Fermi levels leads to the electron flow from g-C 3 N 4 to TiO 2– x , which increases the electron-deficient sites in g-C 3 N 4 . As a result, the cleavage of O–O bonds on the surface of g-C 3 N 4 is avoided and the oxygen adsorption configuration is tuned from Yeager-type to Pauling-type (end-on). Consequently, the photocatalytic H 2 O 2 production rate is dramatically improved to 1780.3 μmol h –1 , which is about 5 times higher than that of pristine g-C 3 N 4 . This work paves a new way to tailor the oxygen adsorption configuration by rationally designing S-scheme heterojunction photocatalysts.
研究领域:
材料科学
·
催化化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）