JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
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IF 2.857
Yu-Chun Wang,Wen-Jun Qian,Na-Na Li,Xin-Yuan Hao,Lu Wang,Bin Xiao,Xin-Chao Wang,Ya-Jun Yang
Tea plant (Camellia sinensis) is one of the most economically valuable crops in the world. Anthracnose can affect the growth of leaves and cause serious yield losses of tea. Tea plants are rich in secondary metabolites; however, their roles in resistance to anthracnose are unclear. Herein we compared the contents of total phenolics, catechins, and caffeine in two cultivars with different resistances to anthracnose during Colletotrichum fructicola infection. (−)-Epigallocatechin-3-gallate (EGCG), (+)-catechin (C), caffeine, and critical regulatory genes were induced in C. fructicola-resistant tissues. In vitro antifungal tests showed that caffeine more strongly inhibited mycelial growth than tea polyphenols and catechins. Both electron microscopy and bioactivity analysis results showed that caffeine can affect mycelial cell walls and plasma membranes. Through promoter sequences analysis, a number of stress response-related cis-acting elements were identified in S-adenosylmethionine synthetase and tea caffeine synthase. These results demonstrated that (−)-EGCG, (+)-C, and caffeine may be involved in the resistance of tea plants to anthracnose.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 2.6
Xudong Xia,Rongrui Lu,Nanxing Zhao,Hongming Kong,Fang Yuan,Hesheng Liu,Lianliang Liu,Xiangyang Qi,Qiuping Chen
Summary α-Glycosidase, a critical therapeutic target in type 2 diabetes mellitus (T2DM), can be modulated by inhibitors to reduce postprandial blood glucose levels. This study investigated the inhibitory effects of vine tea ( Ampelopsis grossedentata ) and its key compounds on α-glycosidase. The results demonstrated that fifteen varieties of vine tea extracts inhibited α-glycosidase in a dose-dependent manner, with IC 50 values ranging from 5.25 to 35.8 μg mL −1 . HPLC analysis revealed dihydromyricetin (DHM) as the main compound in vine tea, with an IC 50 value of 262.50 μM. Notably, the synergistic combination of DHM and the antidiabetic drug acarbose (ABS) significantly enhanced inhibitory effectiveness, with the optimal ratio (1:125) yielding a potentiation index (CI) of 0.09. Fluorescence and molecular docking analyses supported the hypothesis that DHM enhanced ABS's inhibitory effect through complex formation and specific interactions, offering a promising option for safer and more effective diabetes therapy.
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BIOLOGY OF REPRODUCTION
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2024
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IF 3.1
Shi Heng,Zhao Xin-Hai,Peng Qin,Zhou Xian-Ling,Liu Si-Si,Sun Chuan-Chuan,Cao Qiu-Yu,Zhu Shi-Ping,Sun Sheng-Yun
Di(2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer known for its toxic effects on the male reproductive system. Green tea polyphenols (GTPs), recognized for their antioxidant and anti-inflammatory properties, have demonstrated protective effects on various organs, but the mechanisms by which GTPs mitigate DEHP-induced testicular damage remain unclear. Healthy male C57BL/6J mice were divided into five groups: control, DEHP, DEHP + GTP treatment, GTP, and oil groups. Testicular histopathological changes were assessed using hematoxylin–eosin (H&E), periodic acid-Schiff (PAS), and Masson staining. Ultrastructural alterations were examined through transmission electron microscopy. High-throughput sequencing was performed to analyze the expression of mRNA, miRNA, and lncRNA and construct an lncRNA–miRNA–mRNA regulatory network for identifying key regulatory axes. Mice in the DEHP group exhibited significant testicular damage, including reduced sperm count, mitochondrial deformation, and endoplasmic reticulum dilation. GTP treatment notably improved testicular structural integrity, restored sperm count, and alleviated mitochondrial and endoplasmic reticulum damage. Additionally, DEHP significantly increased activated CD8+ T cells, which were reduced with GTP treatment. High-throughput sequencing revealed that GTP treatment exerted protective effects through the regulation of six key lncRNA–miRNA–mRNA axes. GTPs significantly protect against DEHP-induced testicular damage, and the lncRNA–miRNA–mRNA regulatory axes play a potential role in this process.
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Insects
·
2025
·Vol. 16
·期 3
·307
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IF 2.9
Fida Hussain Magsi,David R. Hall,Zongxiu Luo,Xiangfei Meng,Chunli Xiu,Zhaoqun Li,Lei Bian,Nanxia Fu,Jianlong Li,Zongmao Chen,Xiaoming Cai
Simple SummaryHelopeltis cinchonaeis an economically important pest of tea plantations in Asia and has recently been reported in various provinces of China, expanding its population range. The bugs feed on growing shoots, sucking the sap and leaving the shoots susceptible to infection by fungi. No known control or monitoring tools have been developed forH. cinchonaeexcept for the excessive application of pesticides. The components of the sex pheromone produced by femaleH. cinchonaehave been identified and synthesized in previous work and have been shown to attract male bugs to traps. This is the first time that the components of the sex pheromone have been identified for a species ofHelopeltisbug. Here, we present the results of field trapping tests to optimize the blend of pheromone components, compare different types of traps, and determine the best position of the trap in order to maximize the catch of male bugs. Traps baited with the optimized lure were used to monitor populations of the bug and showed two distinct generations during the year. These results contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.Helopeltis cinchonaeis an emerging pest of tea and causes severe damage to tea plantations in China. The female ofH. cinchonaehas been reported to produce a sex pheromone consisting of two components, hexyl (3R)-3-acetoxybutyrate and (5R)-1-acetoxy-5-butyroxyhexane, and lures containing the synthetic compounds have been shown to attract maleH. cinchonaeto traps in the field. This is the first time that components of the sex pheromone have been identified for a species ofHelopeltisbug, but their field application has not been evaluated in detail. The present study shows that a blend of both compounds loaded into a polyethylene vial at 0.2 and 2 mg, respectively, caught significantly more maleH. cinchonaebugs than the individual compounds and all the other tested blends. Sticky wing traps baited with the binary blend of compounds at the optimized ratio and dosage caught more bugs than bucket funnel or delta traps, and traps hung at a height of 10 cm above the tea shoots caught more bugs than those at other heights. The optimized traps and lures were used for monitoring the pest and two distinct population peaks ofH. cinchonaewere observed, the first one during mid-May to early June, and the other one in mid-September. Overall, the results of this study contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Mengli Chen,Zhanyi Xu,Guoxing Chen,Peitong Chen,Chunxia Tian,Jiali Qian,Tiefeng Song,Yongfeng Jin,Guonian Zhu,Ru Yan
Numerous essential oils have been well acknowledged as eco-friendly alternatives to combat insect pests due to synthetic insecticide-induced pest resistance and environment pollution. As a highly commercial essential oil, tea tree oil exhibits excellent insecticidal and repellent activities. However, the molecular mechanism of the olfactory system mediating the tea tree oil-induced repellency against insect pests remains unknown. In our study, mosquito was used as a suitable model to examine the molecular mechanism of tea tree oil-induced repellency against insect pests. The results showed that tea tree oil exhibited excellent spatial and oviposition repellency against Aedes aegypti adults and outstanding repellency against larvae, which were conferred by the main constituent terpinen-4-ol. The reduced repellency in the Orco–/– mutant strain revealed that tea tree oil-induced repellency against mosquitoes was dependent on odorant receptor(s). Moreover, we identified one antenna-biased odorant receptor, AaOr96, that was involved in detecting constituents of tea tree oil to elicit repellency, and the predicted protein–ligand complex indicated that AaOr96 interacted with terpinen-4-ol via van der Waals forces from five key residues. Finally, knocking out AaOr96 resulted in a reduced spatial repellency against A. aegypti by tea tree oil and terpinen-4-ol, and a reduced oviposition repellency by terpinen-4-ol, but not by tea tree oil. Our study not only reveals that tea tree oil has great potential in pest management but also provides more insights into the molecular basis of repellency of essential oils.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Hui-Ping Yu,Qian-Qian Xu,Xin Wang,Yuan-Yuan Cui,He-Fang Wang,Cheng-Xiong Yang
In this work, a novel fluorinated magnetic microporous organic network (Fe 3 O 4 @FMON) was exquisitely designed and synthesized for highly efficient and selective magnetic solid phase extraction (MSPE) of fluorinated benzoylurea insecticides (BUs) from complex tea beverage samples. The Fe 3 O 4 @FMON exhibited good extraction for BUs via the pre-designed hydrophobic, π-π stacking, hydrogen bonding and specific F F interactions. A sensitive Fe 3 O 4 @FMON-based MSPE-HPLC-UV method with wide linear range (0.10–1000 μg L −1 , R 2 ≥ 0.996), low limits of detection (0.01–0.02 μg L −1 ), and large enrichment factors (85.6–98.0) for BUs from tea beverage samples was developed. By decorating F elements within MON's networks, the Fe 3 O 4 @FMON characterized good hydrophobicity and chemical stability, which could be reused at least 8 times without decrease of recoveries. This work demonstrated the great prospects of Fe 3 O 4 @FMON for enriching trace BUs from complex substrates and triggered the potential of FMON for sample pretreatment of fluorinated analytes.
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PHYTOMEDICINE
·
2024
·
IF 6.7
Shanyi Yang,Zhijuan Wei,Jichu Luo,Xuancheng Wang,Guanghui Chen,Xuan Guan,Zhiyong She,Wenhui Liu,Ying Tong,Huan Liu,Mingsen Wen,Hongwei Chen,Pingchuan Zhu,Gui Li,Dongling Wang,Lin Huang,Siyi Xu,Danying Chen,Qisong Zhang,Ye Wei
Background Non-alcoholic fatty liver disease (NAFLD) poses a significant global public health concern. Liupao tea (LPT) is a Chinese national geographical indication product renowned for its lipid-lowering properties. However, the precise mechanisms and active constituents contributing to the efficacy of LPT against NAFLD remain unclear. Purpose This study aims to comprehensively explore the therapeutic potential of Liupao tea extract (LPTE) in alleviating NAFLD through an integrated strategy. Methods Initially, network pharmacology analysis was conducted based on LPTE chemical ingredient analysis, identifying core targets and key components. Potential active ingredients were validated through chemical standards based on LC-MS/MS. To confirm the pharmacological efficacy of LPTE in NAFLD, NAFLD mice models were employed. Alterations in hepatic lipid metabolism were comprehensively elucidated through integration of metabolomics, lipidomics, network pharmacology analysis, and real-time PCR analysis. To further explore the binding interactions between key components and core targets, molecular docking and microscale thermophoresis (MST) analysis were employed. Furthermore, to investigate LPTE administration effectiveness on gut microbiota in NAFLD mice, a comprehensive approach was employed. This included Metorigin analysis, 16S rRNA sequencing, molecular docking, and fecal microbiome transplantation (FMT). Results Study identified naringenin, quercetin, luteolin, and kaempferol as the potential active ingredients of LPTE. These compounds exhibited therapeutic potential for NAFLD by targeting key proteins such as PTGS2, CYP3A4, and ACHE, which are involved in the metabolic pathways of hepatic linoleic acid (LA) and glycerophospholipid (GP) metabolism. The therapeutic effectiveness of LPTE was observed to be comparable to that of simvastatin. Furthermore, LPTE exhibited notable efficacy in alleviating NAFLD by influencing alterations in gut microbiota composition ( Proteobacteria phylum, Lactobacillus and Dubosiella genus) that perhaps impact LA and GP metabolic pathways. Conclusion LPTE could be effective in preventing high-fat diet (HFD)-induced NAFLD by modulating hepatic lipid metabolism and gut microbiota. This study firstly integrated bioinformatics and multi-omics technologies to identify the potential active components and key microbiota associated with LPTE's effects, while also primally elucidating the action mechanisms of LPTE in alleviating NAFLD. The findings offer a conceptual basis for LPTE's potential transformation into an innovative pharmaceutical agent for NAFLD prevention.
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Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xiangyan Mo,Fangxue Hang,Er-Fang Ren,Lili Gai,Kai Li,Debao Niu
Camellia oleifera ( C. oleifera ) shells, a major by-product of the camellia oil industry, are rich in tea saponins. The efficient extraction and characterization of tea saponins from C. oleifera shells using deep eutectic solvents (DESs) were investigated, with the extraction mechanism further explored through scanning electron microscopy (SEM) and molecular dynamics simulations. A total of 24 different types of DESs were prepared, with the optimal DES identified as a combination of L-proline and acetamide (the molar ratio of 1:4). Response surface methodology (RSM) optimization revealed that the maximum yield of tea saponins extraction reached 22.46 %. 18 kinds of tea saponin monomers were identified by ultrahigh-performance liquid chromatography coupled with quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). SEM analysis showed that DESs effectively disrupted the surface structure of C. oleifera shell. Furthermore, molecular dynamics simulations demonstrated the high extraction efficiency of DESs was due to their larger solvent accessible surface area (SASA), increased number of hydrogen bonds, extended hydrogen bond lifetime, and lower intermolecular interaction energy between DESs and tea saponins. These findings provide new insights into the extraction mechanism and contribute to the optimization of DES-based extraction techniques for bioactive compounds from natural plant sources.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jie-Qiong Wang,Bang-Ming Tang,Ying Gao,Jian-Xin Chen,Fang Wang,Jun-Feng Yin,Liang Zeng,Wei-Biao Zhou,Yong-Quan Xu
The flavor stability of tea beverages during storage has long been a concern. The study aimed to explore the flavor stability of Longjing green tea beverage using accelerated heat treatment trials, addressing the shortage of lengthy storage trials. Sensory evaluations revealed changes in bitterness, umami, overall harmonization, astringency, and ripeness as treatment duration increased. Accompanied by a decrease in L-values, ΔE and an increase in a and b-values. Seventeen non-volatile metabolites and three volatile metabolites were identified differential among samples by metabolomics, with subsequent correlation analysis indicating associations between sensory attributes and specific metabolites. Umami was linked to epigallocatechin 3,5-digallate and alpha - D -glucopyranose, astringency was correlated with ellagic acid and 1-ethyl-1H-pyrrole. Ripeness showed associations with ellagic acid, 6,7-dihydroxycoumarin, heptanal, and benzaldehyde, and overall harmonization was linked to 6,7-dihydroxycoumarin, β -myrcene, α -terpineol, and heptanal. A series of verification tests confirmed the feasibility of accelerated heat treatment trials to replace traditional storage trials. These results offer valuable insights into unraveling the complex relationship between sensory and chemical profiles of green tea beverages.
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Journal of Agriculture and Food Research
·
2024
·
IF 4.8
Jun Chen,Meng Li,Qianru Gao,Huabing Yang,Tianxiang Zhu,Xiaojuan Zou,Baifei Hu,Hongtao Liu
Vine tea is a traditional Chinese tea that has been used for centuries. It is rich in flavonoids and has excellent health benefits, especially for liver. The aim of this study was to investigate the hepatoprotective mechanism of dihydromyricetin (DMY), a flavonoid mainly contained in vine tea. Six-week-old male C57BL/6J mice were administered different doses of DMY by oral gavage for two weeks. Liver function and physiological indices of mice were assessed, while changes in gut microbiota following DMY treatment were monitored through 16S rDNA gene sequencing. Additionally, metabolic pathways and mechanisms were further explored using RT-qPCR and western blot analysis. The results showed that aspartate aminotransferase and alanine aminotransferase levels were significantly reduced after DMY intervention in mice. Meanwhile, the diversity and abundance of gut microbiota was increased by DMY. Moreover, DMY enhanced gut microbiota-mediated primary and secondary bile acid synthesis pathways. It inhibited the expression of bile acid synthesis rate-limiting enzymes, such as cyp7a1 and cyp27a1 , in mouse liver tissue. However, bile acid-related receptors including fxr , FGFR4, and TGR5 were significantly activated, indicating that DMY inhibited bile acid synthesis in the liver. This negative feedback regulation mechanism, in which DMY participated, maintained bile acid homeostasis in the liver and intestine of mice. In conclusion, DMY modulated the composition of intestinal microbiota and its mediated bile acid homeostasis in mice, thereby exhibiting a protective effect on liver tissue.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Guojian Deng,Lunfang Huang,Wenya Wang,Tianzi Yu,Jingming Ning,Yujie Wang
The intensity of green tea's floral and sweet flavors was enhanced after being scented by osmanthus (OSGT). However, the mechanism of flavor enhancement by key volatiles remains unknown. Here, the role of key volatiles in OSGT on aroma and taste was explored by sensory experiment-guided flavor analysis. Binary mixed models of ( E )- β -ionone, dihydro- β -ionone, and α -ionone showed additive interactions on floral aroma enhancement, the interactions were increased with increasing concentrations. At the concentration in OSGT, binary mixed models of ( E )- β -ionone, geraniol , linalool , and γ -decalactone showed additive interactions on sweet aroma enhancement. ( E )- β -ionone, geraniol , linalool , and γ -decalactone all significantly increased the perceived intensity of sweetness of sucrose solutions. Additionally, molecular docking revealed the perception mechanism of olfactory and taste receptors to the above characterized volatiles, with hydrogen bonding and hydrophobic interactions being the main interactions. This study highlights the importance of characteristic volatiles in enhancing the flavor of OSGT.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jia Yu,Yuanjie Wu,Chaoxi Zeng,Haoren Wu,Gaofeng Jia,Jianhui Ye,Si Qin,Zhonghua Liu,Meng Shi
Dark tea has long been recognized for its health-promoting benefits, attributed to its complex phytochemical composition. However, the specific bioactive compounds responsible for these beneficial effects remain inadequately characterized. This study aimed to explore the impact of dark tea water extract (DTE) on digestive enzyme activity, antioxidant capacity, and glucose-lipid balance. DTE was fractioned into four fractions using gel separation, followed by analysis with high-performance liquid chromatography and quasi-targeted metabolomics. The 30 % ethanol elution (EEA) and 50 % ethanol elution (EEB) fractions showed stronger antioxidant and enzyme inhibition effects compared to the whole DTE. The EEA fraction was rich in 5 catechins and 26 additional phytochemicals, while the EEB fraction contained high levels of caffeine, ECG, and 29 other phytochemicals. Notably, significant correlations were observed between quercetin-3,4′-O-di-β-glucopyranoside and 3-(2-Naphthyl)-L-alanine with digestive enzyme inhibition. Cellular studies revealed the ability of EEA and EEB to reduce lipid accumulation, improve glycolipid metabolism, and alleviate oxidative stress by increasing SOD, CAT, and GSH levels while decreasing MDA and ROS in HepG2 cells. Furthermore, 34 flavonoids, 2 alkaloids, 2 terpenes, 2 alcohols and polyols, 2 phenylpropanoids and polyketides, 1 organoheterocyclic compound were directly linked to the antioxidant activity and the modulation of glucose and lipid levels. These findings offer valuable insights into the phytochemical profiles of dark tea and its potential health benefits.
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