Nature Communications
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2026
·Vol. 17
·期 1
·2821
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IF 18.1
Xiao Hai-Tao,Song Peng,Jin Jing,Wang Yong-Dan,Xu Yin-Yue,Wu He,Shao Xue-Xue,Gan Qi-Feng,He Jian,Wen Yan-Rong,Zhu Jing,Chen Bao-Yi,Zhou Ping,Lai Li-Na,Yang Hua,Li Ping,Zheng Zu-Guo
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a global health concern that affects nearly one-quarter of the world’s population. General control non-repressed protein 5 (GCN5), a histone acetyltransferase (HAT), has been implicated in the progression of several diseases, but its role in MASLD remains unclear. Here, we provide the experimental evidence that progressive human and male murine MASLD is driven by GCN5, but not by p300/CREB binding protein associated factor (PCAF) activation. Hepatocyte-specific GCN5 overexpression accelerates MASLD progression, whereas its ablation alleviates disease severity. Moreover, pharmacological inhibition of GCN5 with CPTH2 protects against MASLD. Metabolomics and RNA-seq analyses demonstrate that GCN5 promotes de novo lipogenesis (DNL) by upregulating SREBP1c-mediated transcription of lipogenic genes. Mechanistically, GCN5 acetylates histone H3 at the SREBP1c promoter, enhancing transcription through its intrinsic acetyltransferase activity. Our findings further identify GCN5 as a key regulator of LXRα-induced SREBP1c expression, suggesting that targeting GCN5 may selectively inhibit SREBP1c-driven DNL without impairing LXRα-mediated reverse cholesterol transport (RCT). Notably, combined treatment with the Liver X Receptor (LXR) agonist T0901317 and CPTH2 synergistically reduced lipid accumulation in vitro and in vivo, highlighting a promising therapeutic strategy for MASLD.
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Journal of Animal Science and Biotechnology
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2026
·Vol. 17
·期 1
·77
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IF 7.9
Li Yanqing,Fu Min,Yu Lijuan,Zhang Zhen,Xue Zhiyong,Huang Lanhui,Olsen Rolf Erik,Yao Yuanyuan,Yang Yalin,Ran Chao,Wang Chen,Ding Qianwen,Zhou Zhigang
Background The application of traditional vitamin C (VC) and sodium acetate (SA) in aquafeeds is hampered by significant limitations. VC suffers from poor stability during feed processing and storage, as well as low bioavailability. SA is highly hygroscopic, thermally unstable, and rapidly absorbed, leading to short duration of action. This study aimed to design, synthesize, and evaluate tetraacetyl vitamin C ester (TVCE), a novel VC-acetic acid conjugate, as a superior alternative. Results TVCE was successfully synthesized via a one-step acetylation reaction with high yield (90%) and purity (> 95%). By optimizing the distillation recovery process, the by-product acetic acid from the reaction was efficiently recovered and recycled. TVCE demonstrated excellent lipophilicity, remarkable thermal stability (withstanding 160 °C), and was efficiently hydrolyzed by pancreatic lipase in simulated intestinal fluid, indicating its function as a prodrug. In zebrafish, TVCE enhanced systemic antioxidant capacity (increasing superoxide dismutase activity and total antioxidant capacity, while decreasing malondialdehyde content) comparably or superiorly to sodium VC-2-phosphate, even at a lower VC-equivalent dosage. In tilapia, dietary inclusion of TVCE, particularly at 0.1%, significantly improved the weight gain rate and survival rate ( P < 0.05). Notably, despite providing only 48% of the acetate equivalent found in the physical mixture (sodium VC-2-phosphate + SA) group, TVCE still demonstrated superior performance. Furthermore, TVCE supplementation ameliorated liver and intestinal health, as evidenced by reduced serum alanine aminotransferase, diamine oxidase levels, and liver total triglyceride levels, improved tissue morphology, and an optimized gut microbiota structure characterized by an increased abundance of Bacteroidota. Conclusions TVCE is successfully synthesized through a simple, green, and environmentally friendly process, which has successfully achieved the co-production of TVCE and acetic acid. TVCE effectively overcomes the stability and bioavailability issues of conventional VC and SA. In vivo studies demonstrated that TVCE significantly enhances growth performance, antioxidant status, and liver-intestine health in fish. These benefits are attributed to the synergistic effects of VC and acetic acid released upon hydrolysis, which results in a higher biological titer and achieving a "1 + 1 > 2" outcome. These findings establish TVCE as a promising multi-functional nutritional precursor with significant application potential in aquatic feeds.
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Scientific Reports
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2026
·
IF 4.9
Tang Cuiping,Bai Jie,Liu Xinqi,He Yong,Chen Yong,Zang Xiaoya,Guan Jinan,Varfolomeev Mikhail A.,Liang Deqing
The anti-hydrate surface is considered as a promising solution to prevent gas hydrate blockages. Previously, superhydrophobic surfaces have been investigated. But their surface micro structures are easily damaged and lack durability, thereby leading to promote hydrate nucleation and increase the adhesion of hydrates to the wall. In this work a hydrophilic dual network gel coating Zr 4+ /D-Gel was proposed and tested for the inhibition tetrahydrofuran (THF) hydrates formation as an example and for the reduction of the adhesion force of hydrates on the wall surface. In comparison to the uncoated surface, the Zr 4+ /D-Gel hydrophilic coating significantly prolongs the induction time for THF hydrate formation and diminishes the adhesion strength of THF hydrates. Moreover, THF hydrates in contact with the Zr 4+ /D-Gel coating exhibit a cycle of formation and decomposition over time, and a distinct unfrozen water layer was observed at the solution edges during hydrate formation. This work may provide novel insights and direction for the safe and efficient exploitation and transportation of natural gas hydrates.
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Scientific Reports
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2026
·
IF 4.9
Liu Jiaying,Liu Nan,Ye Yiting,Ye Zhandong,Jiang Dongxu,Huang Song
Precancerous gastric lesions (PLGC) represent a critical transitional stage toward gastric cancer, yet effective interventions to halt progression remain limited. Vitexin, a naturally occurring flavonoid, exhibits anti-cancer potential; however, its molecular targets and mechanisms in PLGC have not been fully defined. This study aimed to elucidate the targets and mechanisms of vitexin against PLGC using an integrative strategy combining network pharmacology, molecular docking/molecular dynamics (MD), and cellular validation. Putative targets of vitexin and PLGC-associated genes were intersected to obtain 33 common targets, followed by protein–protein interaction network construction and pathway enrichment analyses. Molecular docking was performed against core targets, and MD simulations were conducted to assess the stability of the EGFR–vitexin complex. In vitro validation was carried out in MNNG-induced MC cells using cell viability, migration, and invasion assays, phase-contrast imaging, and immunofluorescence staining of EGFR and E-cadherin. Network analysis highlighted CCND1, EGFR, and ABL1 as central nodes and implicated oncogenic programs including cell-cycle regulation and PI3K–Akt signaling pathway. Docking suggested favorable binding of vitexin to the core targets. MD simulations further supported a stable binding mode of vitexin within the EGFR pocket over 100 ns with preserved global compactness; a late-stage conformational adaptation accompanied by hydrogen-bond network reorganization was observed without signs of global unfolding. Functionally, vitexin reduced MC cell viability in a dose-dependent manner, reaching ~ 50% inhibition at 20 μM, and significantly suppressed cell migration and invasion. Immunofluorescence demonstrated decreased EGFR expression and altered membrane localization, together with restored E-cadherin expression and membrane localization, suggesting a shift toward an epithelial phenotype. Vitexin inhibits PLGC-associated malignant phenotypes through multi-target modulation. Among the identified candidates, EGFR may serve as a putative and functionally relevant target, while modulation of EGFR-associated signaling together with restoration of E-cadherin may contribute to the reversal of EMT-related phenotypes. These findings provide mechanistic and structural support for the further preclinical evaluation of vitexin in preventing gastric cancer progression.
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Nature Communications
·
2026
·
IF 18.1
Wang Zihao,Cheng Quhan,Zhou Mengxue,Feng Kexin,Chen Qi,Yuan Yi,Zhang Shengmin,Kong Deling,Wang Kai,Wang Jianglin
Erythrocytes and erythrocyte membrane vesicles have shown promise for personalized drug delivery through passive immune evasion, yet their active role in immune phagocytosis remains largely unexplored. Here, we introduce a paradigm based on phosphatidylserine-everted erythrocyte membrane vesicles that trigger efferocytosis, enabling immune-mediated tissue regeneration rather than immune evasion. Incorporation of these phosphatidylserine-everted erythrocyte membrane vesicles into small-diameter vascular grafts markedly enhances endothelialization, suppresses calcification, and improves long-term patency in a rabbit carotid artery replacement model. Preclinical evaluation in a large canine model further demonstrates superior performance compared with clinically used expanded polytetrafluoroethylene grafts. This study establishes engineered phosphatidylserine-everted erythrocyte membrane vesicles as a versatile, customizable, and cost-effective biointerface for vascular grafts and other blood-contacting devices, providing a compelling strategy to harness innate immune mechanisms for regenerative medicine.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Xiao Le,Zheng Yuanyuan,Jiang Xingyi,Wang Manyi,Luo Yanbo,Pang Yongqiang,Wang Hongjuan,Chen Di
This study presents a liquid-filled stick microextraction method with a built-in acidifier (LFSME-BA) that enables ready-to-use, pH-adjustment-free extraction of non-steroidal anti-inflammatory drugs (NSAIDs) from complex herbal matrices. Similar to an alcohol swab, the device contains an H 3 PO 4 -saturated hydrophobic deep eutectic solvent (DES) loaded inside a hollow plastic stick and a polypropylene fabric tip. Upon use, the acidified DES is released and absorbed by the fabric tip. When immersed in the sample solution, H 3 PO 4 gradually diffuses outward and, in the aqueous model solution used in this study, reduces the sample pH to approximately 1.97. This in situ acidification converts the NSAIDs from their ionized forms to their neutral molecular species, markedly increasing hydrophobicity, thereby facilitating their diffusion into the DES phase. Following desorption with 0.4 mL acetonitrile and LC–UV analysis, the LFSME-BA method provided excellent linearity ( R 2 ≥ 0.994, 20–2000 ng/mL), low detection limits (3.04–5.80 ng/mL), and satisfactory accuracy (93.7–102.5%) and precision (RSD ≤ 8.3%). Application to herbal oral liquids and granules yielded relative recoveries of 85.1%–108.8%, further confirming the method’s reliability. This ready-to-use, disposable platform combines extraction and pH self-regulation in one step, offering a simple and efficient approach for screening adulterated pharmaceuticals in herbal products. Graphical
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CHINESE JOURNAL OF POLYMER SCIENCE
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2026
·
IF 4.6
Yang Yi,Song Jun-Mei,Wang Tian-You,Wu Shu-Wei,Zhou Yu-Hang,Gu Zhi-Peng,Guo Ling-Hong,Jiang Xian
Sanshool is a promising skin photoprotective agent with strong UV absorption and great antioxidative activity. However, it faces challenges including poor stability, skin penetration-associated systemic toxicity, and efficacy loss upon chemical modification. To address these issues, amphiphilic hyaluronic acids (HHA) were synthesized and self-assembled to integrate with sanshool via hydrophobic interactions, significantly boosting its photostability by 24% and enhancing its antioxidative activity. In HaCaT cells, HHA-sanshool nanoparticles (NPs) reduced UVB-induced reactive oxygen species, decreased cell apoptosis, and lowered G2/M phase arrest from 42% to approximately 31% (close to the normal level), while also inhibiting excessive autophagy. Moreover, in a mouse model, HHA-sanshool NPs alleviated UVB-induced skin damage, reducing skin thickening by up to 50% and mitigating erythema, protected collagen/elastic fibers, and suppressed proinflammatory factor, with no dermal penetration in vivo . This strategy provides a simple, efficient and safe platform for natural active molecular clinical translation in skin photoprotection.
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Journal of Food Measurement and Characterization
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2026
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IF 3.6
Zhao Mengyuan,Liu Xiaofei,Liu Yang,Li Wanxuan,Zhang Xiangrong
The aim of this study was to encapsulates cinnamaldehyde (CA) using the complex coacervation technique with soybean protein isolate (SPI) and maltodextrin (MD) as the wall matrix. The physical characteristics of cinnamaldehyde microcapsules (CM) were analyzed by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), molecular docking, and thermogravimetric analysis (TG). The results demonstrate that SPI and MD successfully encapsulated CA via hydrogen bonding and electrostatic interactions. The composite exhibited a smooth surface without voids, thereby preventing the rapid volatilization of CA. The thermal stability of CM was significantly improved compared with free CA. CA could be sustainedly released over a period of 10 days under different conditions. On the 10th day, the cumulative release rates of CM stored at 4 ℃, 25 ℃ and 46 ℃ were 39.99%, 48.16% and 76.36%, respectively. Meanwhile, the cumulative release rates of the microcapsules stored at 33%, 52% and 77% relative humidity were 45.97%, 61.51% and 65.90%, respectively. Its release followed the first-order kinetic. CM also inhibited the growth of E. coli, S. aureus, and Aspergillus niger. The shelf life of chilled chicken breast was effectively extended by 9 days upon CM treatment. Compared with the control group, chicken breast treated with CM showed lower pH, reduced thiobarbituric acid reactive substances (TBARS) to 0.14 mg/kg, and decreased total volatile base nitrogen (TVB-N). The research provides a valuable information to prepare CA to preserve meat.
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AQUACULTURE INTERNATIONAL
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2026
·Vol. 34
·期 5
·140
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IF 3.5
Shehata Akram Ismael,El Basuini Mohammed F.,Shija Vicent Michael,Bakky Md. Akibul Hasan,Tran Ngoc Tuan,Zhang Ming,Li Shengkang
This study probed the impacts of dietary octacosanol supplementation at 0 (control), 6, 12, and 18 mg/kg diets on the growth parameters, intestinal enzyme activities, biochemical, antioxidant status, gene expression pattern, and hypoxia tolerance of whiteleg shrimp ( Litopenaeus vannamei , initial weight 1.49 ± 0.01 g, n = 3, total shrimp = 360) during a 60-day feeding trial. To assess growth efficiency, final weight, weight gain, and specific growth rate were recorded, which were significantly ( p < 0.05) improved with octacosanol supplementation, with optimal responses observed at 12 mg/kg. Feed conversion ratio decreased quadratically, reaching optimal efficiency at approximately 10.83 mg/kg. Intestinal enzyme activities (amylase, lipase, and protease) and antioxidant indicators (SOD, CAT, GPx, and TAC) were markedly enhanced at 12 mg/kg, while lipid peroxidation (MDA) was minimized. Hemolymph biochemical indices, including total protein, albumin, globulin, HDL-c, and lysozyme, increased significantly ( p < 0.05), whereas AST and ALT decreased. Furthermore, transcriptional expression of immune- and antioxidant-related genes ( Mn-SOD , CAT , GSH-Px , LYZ , and ProPO ) was upregulated, coinciding with elevated antioxidant capacity and immune responses. Shrimp fed octacosanol diets exhibited enhanced hypoxia tolerance, with LT 50 extended by approximately 30–40 min compared to the control. Collectively, the results demonstrate that dietary octacosanol at 12 mg/kg optimizes growth, physiological health, and stress resilience in L. vannamei , supporting its potential as a natural dietary additive for sustainable shrimp farming. Future studies are needed to explore octacosanol’s long-term effects on shrimp under various stress conditions, its molecular mechanisms, and its impact on other species, as well as its synergy with other feed additives.
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Nature Communications
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2026
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IF 18.1
Shi Yuanji,Yang Minghao,Mu Hongxin,Hu Haidong,Ren Hongqiang
The global shift in per- and polyfluoroalkyl substance (PFAS) crisis towards (ultra-)short-chain congeners highlights the urgent need to design adsorbents with hydrophilic functions. However, current functional group immobilization modifications fail to balance efficiency with regeneration, limiting access to affordable (ultra-)short-chain PFAS-free potable water. Herein, we present a non-immobilized dynamic hydroxyl cycling strategy that uses only water to circularly drive commercial zeolite adsorption-regeneration processes, efficiently and sustainably removing various (ultra-)short-chain PFASs (C-F number: 1-6) from potable water. The enhanced nanopore accessibility and the formation of a “zeolite framework-confined water” dual-binding mode make the modified zeolite one of the highest capacity adsorbents reported (233.82-733.13 mg g -1 ). Notably, this methodology offers a potential low-cost, in-situ upgrade for existing purifiers, potentially providing healthy water to underdeveloped regions through 179 days of full-scale purification.
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CELLULAR & MOLECULAR BIOLOGY LETTERS
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2026
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IF 12.2
Xiaoxuan Zhao,Yang Zhao,Yuepeng Jiang,Yiming Ma,Jing Ma,Hongli Zhao,Xiaoling Feng
Background Unexplained recurrent spontaneous abortion (URSA) is characterized by defective endometrial stromal cell decidualization, with cellular senescence emerging as a key contributor. However, the metabolic–epigenetic mechanisms linking glycolysis to senescence-driven decidualization failure remain unclear. This study elucidates how hexokinase 2 (HK2)-mediated glycolytic reprogramming promotes histone lactylation-dependent stromal senescence and decidualization impairment in URSA. Methods We employed multi-omics profiling (RNA-seq, metabolomics, and CUT&Tag) of primary stromal cells from patients with URSA and controls to map the histone H3K18 lactylation (H3K18la)–cut-like homeobox 1 (CUX1)–senescence-associated secretory phenotype (SASP) axis. Subsequently, this axis was validated both in vitro decidualization models and URSA murine models. Results Decidual tissues from patients with URSA exhibited stromal cell senescence and impaired decidualization. Mechanistically, HK2-driven glycolysis elevated lactate production, which in turn promoted H3K18la at the CUX1 promoter. CUX1 then directly activated the transcription of key SASP factors, thereby propagating the senescence state. Critically, CUX1 depletion or glycolysis inhibition rescued these senescence and decidualization deficiency in vitro. Furthermore, CUX1 knockdown in the URSA murine model reduced stromal senescence and improved decidualization. Conclusions Our findings define a novel HK2–H3K18la–CUX1–SASP signaling axis that drives URSA pathogenesis by linking metabolic reprogramming with epigenetic regulation. This work highlights CUX1 as a potential therapeutic target for correcting decidualization deficiency in URSA. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1186/s11658-026-00879-y.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Liu Tao,Li Dan,Hu Wenying,Neha,Mohan Brij,Sun Wei
The need for sensitive and reliable adenine (Ade) detection in complex biological systems has driven the development of advanced electrochemical sensing platforms. In this work, we developed a molecularly imprinted electrochemical sensor based on a ternary interface composed of homogeneously distributed Au nanoparticles (AuNPs), nitrogen/sulfur co-doped graphene quantum dots (NSGQDs), and nitrogen-doped graphene (NGR) on disposable screen-printed electrodes. Unlike conventional conductive modifiers that primarily promote electron transfer, NSGQDs served as nucleation-directing mediators, regulating the growth and anchoring of AuNPs on NGR and thereby producing a more uniform, lower-resistance transduction layer. This improved interfacial homogeneity facilitated signal transduction and supported the formation of a more consistent poly(o-phenylenediamine) molecularly imprinted film for Ade recognition. Under optimized conditions, the sensor exhibited two linear ranges of 0.05–20.0 μM and 20.0–150.0 μM, with a limit of detection of 0.012 μM and a limit of quantification of 0.036 μM. The sensor also showed good selectivity, reproducibility, reusability, and stability, with satisfactory recoveries in spiked human serum samples. These results indicate that improved control over AuNPs nucleation and interfacial uniformity can translate into broader linearity, lower detection limits, and more dependable electrochemical sensing of Ade in complex matrices. Graphical abstract
NSGQDs drive uniform formation of AuNPs on N-doped graphene, creating a fast-transport transducer for poly(o-phenylenediamine)-imprinted adenine sensing with high selectivity, low Rct, and serum-level applicability.
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