Materials
·
2021
·Vol. 14
·期 17
·4895
·
IF 3.623
Linqing Zuo,Haodong Sun,Xinhai Yuan,Juan Wen,Xi Chen,Shiyu Zhou,Yuping Wu,Teunis van Ree
The Zn/MnO2battery is a promising energy storage system, owing to its high energy density and low cost, but due to the dissolution of the cathode material, its cycle life is limited, which hinders its further development. Therefore, we introduced agar as a microskin for a MnO2electrode to improve its cycle life and optimize other electrochemical properties. The results showed that the agar-coating layer improved the wettability of the electrode material, thereby promoting the diffusion rate of Zn2+and reducing the interface impedance of the MnO2electrode material. Therefore, the Zn/MnO2battery exhibited outstanding rate performance. In addition, the agar-coating layer promoted the reversibility of the MnO2/Mn2+reaction and acted as a colloidal physical barrier to prevent the dissolution of Mn2+, so that the Zn/MnO2battery had a high specific capacity and exhibited excellent cycle stability.
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JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2019
·
IF 4.027
Wen-Jia Dan,Qiang Zhang,Fan Zhang,Wei-Wei Wang,Jin-Ming Gao
In this article, 23 compounds (6 and 7a–7v) were prepared and evaluated for their in vitro α-glucosidase inhibitory activity. The compounds 7d, 7f, 7i, 7n, 7o, 7r, 7s, 7u, and 7v displayed the α-glucosidase inhibition activity with IC50 values ranging from 1.68 to 7.88 µM. Among all tested compounds, 7u was found to be the most efficient, being 32-fold more active than the standard drug acarbose, which significantly attenuated postprandial blood glucose in mice. In addition, the compound 7u also induced the fluorescence quenching and conformational changes of enzyme, by forming α-glucosidase–7u complex in a mixed inhibition type. The thermodynamic constants recognised the interaction between 7u and α-glucosidase and was an enthalpy-driven spontaneous exothermic reaction. The synchronous fluorescence and CD spectra also indicate that the compound 7u changed the enzyme conformation. The findings identify the binding interactions between new ligands and α-glucosidase and reveal the compound 7u as a potent α-glucosidase inhibitor.
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Frontiers in Nutrition
·
2025
·
IF 5.1
Xiaojie Zhang,Lan Yang,Haifei Lian,Zixuan Xu,Zichen Yun,Xiaohui Xu,Shixiong Liu,Qifei Luo,Majigsuren Zolzaya,Dacheng Liu
Background: Composite yeast culture (CYC) serves as an important functional feed additive. The content of its active ingredient, α-mannan, directly affects product quality and efficacy. However, due to the complex matrix of CYC, establishing a highly specific and accurate quantitative method for α-mannan remains a challenge.Methods: This study aimes to establish an analytical method based on enzymatic hydrolysis combined with high-performance liquid chromatography equipped with an ultraviolet detector (HPLC-UV) for the quantification of α-mannan in CYC. The method involved the specific enzymatic hydrolysis of α-mannan in the sample using α-mannosidase, followed by the release of mannose, which was then derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP) and analyzed by HPLC-UV. The actual content of α-mannan was calculated using a formula.Results: Method validation results showed that mannose exhibited good linearity within the concentration range of 0.5–400 μg/mL, with a correlation coefficient of 0.99998. The limit of detection (LOD) and limit of quantification (LOQ) were 0.063 mg/L and 0.208 mg/L, respectively. The average recovery rate was 88.020%−94.204%, with a relative standard deviation (RSD) ranging from 0.702% to 2.259%, indicating that the method has high accuracy and good precision.Conclusion: This study successfully established an efficient and reliable HPLC-UV detection method suitable for the specific quantitative analysis of α-mannan in CYC. The method exhibited good reproducibility and operational feasibility, providing robust technical support for quality control of CYC products and related functional component research.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 11
·747
·
IF 5.4
Zhang Zhilin,Pu Jin,Peng Yong,Tan Hongliang,Xu Fugang
A noble-metal-free, aggregation-independent surface-enhanced Raman scattering (SERS) platform is reported based on a bifunctional α-MoO 3−x -CuCl 2 nanocomposite for the sensitive and selective detection of phosphate species. The defect-rich α-MoO 3−x provides strong SERS enhancement, and the introduction of CuCl 2 not only enhances its peroxidase like activity, but also endows it selective response to phosphate species. The composite α-MoO 3−x -CuCl 2 can efficient catalytic oxidation of colorless tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB), which yields a strong SERS signal. At the assay pH of 4.0, the protonated phosphate species (mainly H 2 PO 4 ⁻) selectively coordinate with Cu 2+ , suppressing the nanozyme activity and causing a dose-dependent decrease in both colorimetric and SERS responses. Under optimized conditions, the method exhibits a linear detection range from 8.0 × 10 − 9 M to 8.0 × 10 − 5 M and a limit of detection as low as 2.0 × 10 − 9 M. The platform also demonstrates good anti-interference capability against common coexisting ions and satisfactory recoveries (103.75%–107.5%) in spiked lake water and tap water samples. This work provides a low-cost, reliable, and reproducible strategy for indirect SERS detection of phosphate species without reliance on noble metals or nanoparticle aggregation. Graphical abstract
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PLoS One
·
2016
·Vol. 11
·期 3
·e0150412
·
IF 3.057
Sheng Geng,Sharui Shan,Hanjun Ma,Benguo Liu
In order to investigate polymeric flavonoids, the polycondensate of catechin with glyoxylic acid (PCG) was prepared and its chemically antioxidant, cellular antioxidant (CAA) and α -glucosidase inhibitory activities were evaluated. The DPPH and ABTS radical scavenging activities and antiproliferative effect of PCG were lower than those of catechin, while PCG had higher CAA activity than catechin. In addition, PCG had very high α -glucosidase inhibitory activities (IC 50 value, 2.59 μg/mL) in comparison to catechin (IC 50 value, 239.27 μg/mL). Inhibition kinetics suggested that both PCG and catechin demonstrated a mixture of noncompetitive and anticompetitive inhibition. The enhanced CAA and α -glucosidase inhibitor activities of PCG could be due to catechin polymerization enhancing the binding capacity to the cellular membrane and enzymes.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2022
·
IF 6.364
Jinglin Gao,Hongquan Cao,Xiaoyu Hu,Hengyang Mao,Xianfu Chen,Minghui Qiu,Hendrik Verweij,Yiqun Fan
Porous α-quartz ceramics with improved mechanical strength were fabricated by using silica sol as a bonding agent in an aqueous gel-casting process. α-quartz dispersion was prepared with a solid load of 75 wt% and a viscosity of 1.66 Pa·s. α-Al 2 O 3 particles were added to promote dispersion of the bonding agent. The optimum amounts of amorphous silica and α-Al 2 O 3 were 10 % and 8 % of the mass of α-quartz powder, respectively. The thermal treatment of α-quartz green bodies was optimized for mechanical and permeation properties. Electrical fields up to 1 kV/mm were applied to the green bodies to accomplish α-quartz grains orientation and hence piezoelectric response. This caused an increase of piezo-acoustic response by 58 % at an excitation frequency of 200 kHz. In-situ mitigation of fouling was obtained by applying an alternating voltage of 60 V, during TiO 2 suspension filtration, which resulted in an increase of the stationary flux by 23 %.
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Marine Drugs
·
2024
·
IF 4.9
Xin Sun,Jiangnan Hu,Maomao Ren,Hong Chang,Dongting Zhangsun,Baojian Zhang,Shuai Dong
α-Conotoxins, as selective nAChR antagonists, can be valuable tools for targeted drug delivery and fluorescent labeling, while conotoxin-drug or conotoxin-fluorescent conjugates through the disulfide bond are rarely reported. Herein, we demonstrate the [2,4] disulfide bond of α-conotoxin as a feasible new chemical modification site. In this study, analogs of the α-conotoxin LsIA cysteine[2,4] were synthesized by stapling with five linkers, and their inhibitory activities against human α7 and rat α3β2 nAChRs were maintained. To further apply this method in targeted delivery, the alkynylbenzyl bromide linker was synthesized and conjugated with Coumarin 120 (AMC) and Camptothecin (CPT) by copper-catalyzed click chemistry, and then stapled between cysteine[2,4] of the LsIA to construct a fluorescent probe and two peptide-drug conjugates. The maximum emission wavelength of the LsIA fluorescent probe was 402.2 nm, which was essentially unchanged compared with AMC. The cytotoxic activity of the LsIA peptide-drug conjugates on human A549 was maintained in vitro. The results demonstrate that the stapling of cysteine[2,4] with alkynylbenzyl bromide is a simple and feasible strategy for the exploitation and utilization of the α-conotoxin LsIA.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Gu Zhuo,Fu Xiong,Wang Ping-Ping,Tan Chin-Ping,Chen Chun
Sugarcane, as one important and heavily planted industrial crop, is meaningful to develop its byproducts. In this paper, the ultrasonic collaborative pulse was beneficial for the yield improvement and good bioactivity protection. The sugarcane polyphenol extract (SPE) yield reached 2.42 ± 0.08 mg/g DW at the optimized conditions: pulse time of 60 s, pulse intensity of 2 kV/cm, ultrasonic time of 90 min, and ultrasonic power of 120 W. The SPE contained the total phenolic content of 6.01 ± 0.12 mg GAE/g extract and total flavonoids content of 7.15 ± 0.24 mg RE/g extract. The SPE was mainly composed of chlorogenic acid, schaftoside, hyperoside, quercitrin, and trans-3-hydroxycinnamic acid with 10.24 %, 14.92 %, 4.22 %, 12.05 %, 25.54 %, respectively. The SPE showed good radical scavenging activity with ORAC value of 134.57 μmol/g. The SPE could reduce the oxidative stress and extend the mean lifespan of nematodes by 7.19 % in vivo through increasing the activity of SOD and CAT to decrease the ROS level and MDA content. In addition, the SPE showed strong α-glucosidase inhibitory activity with IC50 of 0.53 mg/mL in a mixed inhibition type, which suggested that the SPE had good hypoglycemic potential.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qing Guo,Wei Su,Fangzhou Wen,Jinyun Cai,Lini Huo,Haiyi Zhong,Peiyuan Li
In this paper, we designed a novel “Freeze-thaw” type hydrogel microneedle (PP-CDLut-AMY MN). The “Freeze-thaw” cycle endows the MN excellent water absorption, with a dissolution rate of up to 486 %. The addition of polydopamine@polypyrrole (PP) enabled the MN to have a stable temperature increase to approximately 50 °C under near-infrared light irradiation, which exhibited killing rates of 99 % and 98 % against free S. aureus and E. coli , respectively. Natural macromolecule α-Amylase (AMY) was used as a bacterial biofilm disintegrator, and the destruction rate of S. aureus biofilm reached 83.2 %. Meanwhile, the incorporation of Hydroxypropyl-β-cyclodextrin @Luteolin (CDLut) provided the MN with good antioxidant properties, which could scavenge 73.35 % of DPPH free radicals. In vivo experiments have shown that the MN can effectively promote the healing of wounds infected by S. aureus biofilm and that the stable and gentle photothermal effect did not cause unnecessary damage to the surrounding tissues. We believe that this novel hydrogel MN has great potential to combat bacterial biofilms associated with wound infections.
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BLOOD
·
2025
·Vol. 145
·期 10
·1074-1088
·
IF 23.1
Yong Long,Qianqian Zhang,Ling Ling,Yuan Zhuang,Xiaolei Wei,Haoyang Huang,Zhanping Lu,Yushan Huang,Xianming Chen,Yuhua Ye,Xiaoqin Feng,Haokun Zhang,Binbin Huang,Yueyan Huang,Yidan Liang,Mingyan Fang,Yukio Nakamura,Bin Lin,Xinhua Zhang,Daru Lu,Xin Jin,Xiangmin Xu
Accumulation of free α-globin is a critical factor in the pathogenesis of β-thalassemia. Autophagy plays a crucial role in clearing toxic free α-globin, thereby reducing disease severity. However, the impact of natural mutations in autophagy-related genes (ATGs) on the phenotypic variability of β-thalassemia remains unclear. In this study, we systematically investigated the relationship between variants in ATGs and disease phenotypes in a cohort of 1022 patients with β-thalassemia, identifying 4 missense mutations in the autophagy and beclin 1 regulator 1 (AMBRA1) gene. Disruption of the Ambra1 gene in β-thalassemic mice was found to reduce autophagic clearance of α-globin in red blood cell precursors, exacerbating disease phenotypes. Functional characterization of the AMBRA1 gene and these mutations in patient-derived CD34+ cells, edited human umbilical cord blood–derived erythroid progenitor 2 (HUDEP-2) cells, and engineered HUDEP-2 β-thalassemic cells confirmed that AMBRA1 facilitates the autophagic clearance of free α-globin in human erythroid cells. Functional studies demonstrated that AMBRA1 missense mutants destabilize Unc-51-like kinase 1 protein, inhibit light chain 3 protein lipidation, and subsequently hinder autophagic flux, leading to increased α-globin deposition. Additionally, these mutations were associated with erythrotoxic effects in vitro, including increased intracellular reactive oxygen species levels, higher apoptosis rates, and impaired erythroid differentiation and maturation. This study sheds light on the molecular association between mutations in ATGs and the exacerbation of β-thalassemia, highlighting the potential role of the AMBRA1 gene as a promising diagnostic and therapeutic target for β-hemoglobinopathies.
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Nature Catalysis
·
2023
·Vol. 6
·期 10
·906-915
·
IF 37.8
Li Mengyang,Wu Yongmeng,Zhao Bo-Hang,Cheng Chuanqi,Zhao Jinghui,Liu Cuibo,Zhang Bin
Amino acids have wide application in the food and pharmaceutical industries. Current biotic and chemical syntheses suffer from low efficiency, complex purification operations and high energy consumption. Here we report a sustainable electrocatalytic synthesis of alanine from NO and pyruvic acid over oxide-derived Ag with low-coordination sites under ambient conditions. Mechanistic studies reveal a cascade NO → NH 2 OH → pyruvate oxime → alanine pathway. The quick pyruvate oxime formation and slow pyruvate oxime reduction steps cause various side reactions, leading to low alanine production. Then, a spatially decoupled two-pot electrosynthesis system using flow reactors loaded with oxide-derived Ag is designed for pyruvate oxime formation and reduction reactions. This decoupled system delivers 3.85 g of easily purified alanine with a total Faradaic efficiency of 70% and a purity of >98% at 100 mA cm −2 . Further techno-economic analysis demonstrates the potential. This method is suitable for solar-energy-driven alanine electrosynthesis from polylactic acid wastes and for the fabrication of other amino acids.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2020
·
IF 16.683
Yanxian Geng,Dongyun Chen,Najun Li,Qingfeng Xu,Hua Li,Jinghui He,Jianmei Lu
Heterojunctions have attracted considerable attention for efficiently utilizing solar energy and improving conversion efficiency during pollutant degradation. Herein, carbon nitride and hematite (α-Fe 2 O 3 ) are used to prepare a Z-scheme 2D/2D α-Fe 2 O 3 /g-C 3 N 4 heterojunction using an impregnation-hydrothermal method. The unique 2D/2D structure has a high interfacial area and widely-dispersed active sites. The energy band structure of the Z-scheme heterojunction leads to broad visible-light absorption and promotes charge transfer. Optimizing the content of the α-Fe 2 O 3 precursor in composite leads to a maximum efficiency of 60.8% for the removal of 600 ppb of NO , which is approximately 1.78 times that of g-C 3 N 4 (34.2%). The photocatalytic performance is greatly promoted because of the formation of the heterojunction and the strong interfacial action between g-C 3 N 4 nanosheets and α-Fe 2 O 3 nanoplates. Cycling experiments verify that the α-Fe 2 O 3 /g-C 3 N 4 heterojunction has good stability and reusability. The α-Fe 2 O 3 /g-C 3 N 4 heterojunction therefore has great potential in sustainable and efficient pollutant degradation.
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