Biotechnology for Biofuels and Bioproducts
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2024
·Vol. 17
·期 1
·1-16
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IF 3.3
Wei Xiaoyu,Yang Lijie,Chen Zhen,Xia Wenhao,Chen Yongbin,Cao Mingfeng,He Ning
Background The structural diversity of extracellular polymeric substances produced by microorganisms is attracting particular attention. Poly-gamma-glutamic acid (γ-PGA) is a widely studied extracellular polymeric substance from Bacillus species. The function of γ-PGA varies with its molecular weight (Mw). Results Herein, different endogenous promoters in Bacillus licheniformis were selected to regulate the expression levels of pgdS , resulting in the formation of γ-PGA with Mw values ranging from 1.61 × 10 3 to 2.03 × 10 4 kDa. The yields of γ-PGA and exopolysaccharides (EPS) both increased in the pgdS engineered strain with the lowest Mw and viscosity, in which the EPS content was almost tenfold higher than that of the wild-type strain. Subsequently, the compositions of EPS from the pgdS engineered strain also changed. Metabolomics and RT-qPCR further revealed that improving the transportation efficiency of EPS and the regulation of carbon flow of monosaccharide synthesis could affect the EPS yield. Conclusions Here, we present a novel insight that increased pgdS expression led to the degradation of γ-PGA Mw and changes in EPS composition, thereby stimulating EPS and γ-PGA production. The results indicated a close relationship between γ-PGA and EPS in B. licheniformis and provided an effective strategy for the controlled synthesis of extracellular polymeric substances.
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GREEN CHEMISTRY
·
2025
·
IF 9.2
Chuanya Li,Qianfeng Zhou,Changhu Leng,Yongchao Wang,Chenyang Ma,Junlei Zhang,Zhijun Li
Polyolefin plastics, though highly resistant to degradation due to their thermodynamic stability and chemical inertness, can be catalytically depolymerized into liquid fuels, offering a promising route to mitigate white pollution and fossil fuel dependency. Here, a sustainable catalytic route for closed-loop polyolefin upcycling is achieved using a bifunctional Sn1W9/γ-Al2O3 catalyst that integrates redox and acidic functionalities within a self-regenerative hydrogen relay cycle. The optimized interface between Sn and W species promotes simultaneous C-H dehydrogenation and C-C metathesis via dynamic Sn-H ↔ W-OH coupling, enabling quantitative conversion of polypropylene at 250 o C within 1 hour and selective production of C5-C22 liquid hydrocarbons (95.0%). Structural, spectroscopic, and kinetic analyses identify hydroxylated W 5+ -OH sites as the principal active centers, stabilized by Sn-mediated hydrogen spillover. The catalyst achieves exceptional stability and reusability across multiple degradation cycles and diverse commercial plastics. Life cycle and techno-economic assessments reveal 16-fold enhanced thermal efficiency, > 85% solvent recyclability, and a carbon footprint of 7.3 kg CO2 e•kg⁻¹surpassing benchmark catalysts in both sustainability and energy utilization. This self-sustaining redox-hydroxyl loop establishes a scalable, low-carbon paradigm for circular plastic valorization. Under mild conditions, the system delivers excellent degradation performance and stability, demonstrating scalability for kilogram-scale recovery.
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Foods
·
2025
·Vol. 15
·期 1
·71
·
IF 5.1
Shufeng Yin,Yiyao Wang,RuiXue Zhao,Ning Zhao,Hao Liu,Yining Tang,Ningbo Qin,Yiwei Dai,Xinping Lin
Inoculated fermentation can enhance the flavor, nutrition, and functionality of juice. The lactic acid bacteria (LAB) are commonly used as starter cultures. This study screened LAB for orange juice fermentation and optimized fermentation factors using response surface methodology (RSM) to improve GABA content in orange juice. A total of 52 LAB strains were screened, andLacticaseibacillus paracaseiZY (Lcb. paracaseiZY) andLacticaseibacillus rhamnosusSN12 (Lcb. rhamnosusSN12) presented higher GABA yields and adaptability to substrates. The optimized fermentation factors for GABA enhancement in orange juice were as follows: initial pH of 5.5, fermentation temperature of 37 °C, soluble solids content of 12.0 °Bx, inoculum ratio ofLcb. paracaseiZY toLcb. rhamnosusSN12 as 1:1, inoculum size of 6 Log CFU/mL, and fermentation time of 96 h. Under these optimized conditions, the GABA content reached 0.89 g/L, representing a 39.06% increase compared to uninoculated orange juice. This indicates that RSM-based optimization is conducive to increasing GABA content in orange juice and provides a scientific basis for the development of GABA-enriched functional fermented juices.
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Cell Death & Disease
·
2022
·Vol. 13
·期 4
·1-12
·
IF 9.685
Liu Qihui,Yang Quanli,Wu Zengfeng,Chen Yanfang,Xu Miaomiao,Zhang Hua,Zhao Jiliang,Liu Zonghua,Guan Zerong,Luo Jing,Li Zhi-yong,Sun Guodong,Wen Qiong,Xu Yan,Li Zhenhua,Chen Kebing,Ben Xiaosong,He Wanchun,Li Xueshi,Yin Zhinan,Hao Jianlei,Lu Ligong
Liver fibrosis represents a severe stage of liver damage, with hallmarks of inflammation, hepatic stellate cell activation, and extracellular matrix accumulation. Although previous studies demonstrated γδ T cells are involved in liver fibrosis, the precise role and mechanisms of γδ T cells migrating to fibrotic liver have not been elucidated. Here, we aim to investigate the functional subsets of γδ T cells in hepatic fibrosis and to further explore the underlying causes and drivers of migration. In this study, we observed that γδ T cells accumulate in fibrotic liver. Adoptive transfer of γδ T, especially Vγ4 γδ T subset, can significantly alleviate liver fibrosis. In addition, CCl 4 treatment also leads to activation of mTOR signaling in γδ T cells. Genetic deletion of the Rictor gene, but not Raptor, in γδ T cells markedly exacerbated liver fibrosis. Mechanistically, CCl 4 -induced liver injury causes macrophage accumulation in the liver, and IL-1β produced by macrophages promotes mTORC2 signaling activation in γδ T cells, which upregulates T-bet expression and eventually promotes CXCR3 transcription to drive γδ T cell migration. Moreover, hepatic γδ T cells ameliorated liver fibrosis by cytotoxicity against activated hepatic stellate cells in FasL-dependent manner, and secrete IFN-γ to inhibit the differentiation of pro-fibrotic Th17 cells. Thus, IL-1β-activated mTORC2 signaling in γδ T cells upregulates CXCR3 expression, which is critical for IFN-γ + γδ T cells migration into the liver and amelioration of liver fibrosis. Our findings indicate that targeting the mTORC2 or CXCR3 in γδ T cells could be considered as a promising approach for γδ T cell immunotherapy against liver fibrosis.
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Advanced Science
·
2025
·Vol. 12
·期 26
·2501875
·
IF 14.1
Bao Xiao,Yuquan Wang,Ning Ding,Haoming Qin,Nannan Shen,Xuchang He,Qihao Sun,Yihui He
Perovskite semiconductor cesium lead bromide (CsPbBr 3 ) has demonstrated great promise as a new-generation gamma-ray detector. However, substantial challenges still present in reproducibly achieving optimal spectroscopic performance. The specific strategy for producing spectroscopic-grade CsPbBr 3 crystals with high reproducibility and uniformity are still not clarified. Herein, efficient zone refining processing is developed for CsPbBr 3 crystals that facilitates impurity segregation to achieve an ultrahigh purity level of ≈1.42 ppm, therefore lowers trap density and balances charge transport. In a typical 30 mm diameter zone-refined CsPbBr 3 ingot, all wafers exhibited remarkable energy resolutions of 6–12% and 3–8% for 241 Am and 57 Co γ-rays under comparable electric fields. The crystals also exhibited an ambipolar charge transport characteristic, resemble to elemental semiconductors, with equivalent hole and electron mobility-lifetime products averaging 5.42 × 10 −3 and 2.27 × 10 −3 cm 2 ∙V −1 , respectively. Consequently, over 95% of wafers achieved energy resolutions below 5% whereas 70% exceeded 3% for 137 Cs γ-rays, demonstrating exceptional reproducibility and uniformity. Notably, a champion energy resolution of 1.3% with an outstanding photopeak-to-Compton (P/C) ratio of ≈5.3 is attained in an ambipolar planar detector. It is anticipated that this work shall expedite scalable manufacturing and practical applications of CsPbBr 3 detectors.
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JOURNAL OF PHARMACEUTICAL SCIENCES
·
2026
·
IF 3.9
Zechen Yan,Geping Wu,Yu Wang,Luyang Zhao,Qiuzheng Du
A γ-cyclodextrin MOF oral nano-delivery system was constructed for mitotane with a 20.04% drug loading. • The system enhanced mitotane solubility by ∼3.8-fold and in vitro anti-tumor efficacy against adrenal carcinoma cells. • In vivo pharmacokinetics showed a 1.71-fold increase in oral bioavailability compared to raw drug. Adrenocortical carcinoma (ACC) is an aggressive cancer with an urgent need for more effective and safer therapies. Mitotane (MT), has its clinical utility compromised by poor aqueous solubility and low oral bioavailability. In response, a novel nano-delivery platform was fabricated by encapsulating MT into γ-cyclodextrin metal-organic framework (γ-CD-MOF), denoted as MT@γ-CD-MOF. The composite was prepared via a solvothermal method. Comprehensive characterization combined with molecular simulation confirmed the successful loading of MT in a molecularly dispersed state within the γ-CD cavities, with a drug loading capacity of 20.04% and an approximately 3.8-fold increase in apparent solubility. In vitro studies demonstrated that MT@γ-CD-MOF suppressed the proliferation and migration of SW-13 ACC and enhanced pro-apoptotic effects by activating Caspase-3 and increasing intracellular reactive oxygen species levels. Importantly, in vivo pharmacokinetic studies in rats revealed a substantial improvement in oral bioavailability, with the area under the concentration-time curve from 0 to 24 h (AUC 0-24 h ) increasing by 1.71-fold compared with raw MT. These results indicate that MT@γ-CD-MOF is an efficient and biocompatible oral delivery platform, offering a promising formulation strategy to enhance the therapeutic efficacy of MT against ACC. Download: Download high-res image (183KB) Download: Download full-size image
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POLYMER DEGRADATION AND STABILITY
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2018
·
IF 3.193
Hui Gao,Fang Xie,Yanju Liu,Jinsong Leng
Recently, shape memory polyimides (SMPIs) with varying glass transition temperature ( T g ) and optical transparency have been developed widely. While, rare reports were focused on the evaluation of the durability of SMPIs in the complex and volatile space environments. This paper presents the simulated γ-radiation accelerated experiment of two kinds of optically transparent SMPIs with a T g of 178 °C and 196 °C, respectively. The effects of γ-radiation on chemical structure , optical transmittance, shape memory behavior, thermomechanical property , thermal stability, and mechanical properties were investigated. Although SMPIs have endured γ-radiation for 1 × 10 6 Gy, the chemical structure, T g , optical transmittance, thermal stability, and the shape memory behavior remained unchanged. Also, SMPIs with a T g of less than 200 °C could maintain their high tensile strengths and elongations at break (>109.5 MPa, >7.6%) after being exposed to 1 × 10 6 Gy γ-radiation, which shows great potential application in space active deformation structures and devices.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2026
·
IF 3.9
Yunxiang Luo,Tongtong Zhang,Yang Qin,Fujie Zhao,Hongchen Wang,Guohua Liu,Lu Qi
Bisphenol A (BPA), an emerging wastewater contaminant, poses significant ecological risks even in trace concentrations, necessitating advanced removal strategies. In this study, a novel γ-Fe2O3-embedded nitrogen-doped polydopamine-derived carbon composite adsorbent (Fe-PC) was synthesized using polydopamine as a self-nitrogen-doped carbon precursor rather than a conventional shell material. The Fe-PC adsorbent exhibited a high BPA adsorption capacity of 423.86 mg g–1 and rapid equilibrium within 120 min. Adsorption kinetics followed a pseudo-second-order model (R2 = 0.999), while equilibrium data aligned with the Langmuir isotherm, indicating monolayer chemisorption is the dominant mechanism. Thermodynamic analysis confirmed that the process is spontaneous and endothermic. Mechanistic investigations revealed that γ-Fe2O3 introduces abundant active sites, enabling synergistic adsorption via chemical bonding, π–π interactions, hydrophobic interactions, and Fe3+ coordination with BPA hydroxyl groups. Combining high adsorption capacity, rapid kinetics, and stability, Fe-PC offers a promising strategy for efficiently removal of emerging contaminants from water.
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POLYMER COMPOSITES
·
2026
·
IF 4.7
Mengfei Du,Enhao Zhu,Xilai Yan,Zhe Wang,Ke Wang
Enhancing the durability of ultraviolet-cured-in-place pipe (UV-CIPP) in alkaline environments remains challenging due to its long-term exposure to concrete pore solution from the original pipe. Herein, a novel strategy that γ-AlOOH coating assists to immobilize Irganox 1010 onto glass fibric (GF) was proposed for simultaneously improving alkaline corrosion resistance and mechanical properties of UV-CIPP. Through the beneficial bridging of γ-AlOOH with the coupling agent, Irganox 1010 was immobilized on the GF (GF-Al-g-1010) surface through hydrogen bonding and covalent bonding. The leaching of Si 2+ from the GF backbone of unsaturated polyester resin (UP)/GF-Al-g-1010 composite was reduced by 40.60% and the hydrolyzed alcohol products decreased by 47.1%. Meanwhile, the mechanical properties of UP/GF-Al-g-1010 remained well preserved. The flexural and tensile modulus retained at 99.0% and 96.3%, respectively. The immobilization of Irganox 1010 at the UP/GF interface formed a protective hydrophobic layer and stabilized free radicals, which together inhibited resin degradation and reduced interface debonding. Moreover, the flexural strength and modulus of composites increased by 36.5% and 38.4%, respectively. The chemical bonding between GF-Al-g-1010 and UP chains enhanced interfacial adhesion. In summary, this work will facilitate the design of UV/thermal dual-cured composites with high strength and alkaline corrosion resistance for pipeline rehabilitation. Highlights A novel AlOOH coating assists to immobilize Irganox 1010 onto glass fabric. The flexural strength and modulus increased 36.5% and 38.4%, respectively. The Si 2+ leaching and hydrolyzed alcohol products were reduced by 40.60% and 47.1%. The flexural and tensile modulus retention reached 99.0% and 96.3%, respectively. The AlOOH-1010 coating improved both durability and mechanical properties.
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CARBON
·
2026
·
IF 11.6
Wenjun Zhang,Youhai Huang,Sifan Zhang,Sanqiang Zhang,Zhaohua Fang,Meng Chen
As a rising new member of carbon allotropes, graphdiyne has shown great potential in various fields due to its fascinating properties. Herein, a controlled two-step hydrothermal route was employed to convert hydrogen-substituted γ-graphdiyne into two functional derivatives: graphdiyne oxides (OGDY) and graphdiyne oxide quantum dots (OGDYQD). Systematic characterization revealed distinct differences in morphology, chemical structure, and optical properties between precursors and derivatives. Especially, the existence of alkyne bonds was demonstrated through evidence from Fourier transform infrared spectroscopy (FTIR), photoluminescence spectroscopy (PL), and selective oxidation experiments, which provides new insights for the characterization of graphdiyne materials. Moreover, we identified an oxidation-locking phenomenon governing selective alkyne oxidation at specific sites during synthesis. The oxidation-locking feature endowed significant application potential upon both derivatives: OGDY exhibited excellent photocatalytic ability in the degradation of cationic dyes, achieving efficiency ∼5–200 higher than those of other carbon-based materials; while OGDYQD could achieve ultrasensitive electrochemical detection of methyl orange with a limit of detection (LOD) as low as 0.032 μM, as well as serve as an excellent catalyst, which was comparable to metal catalysts, for the hydrogenation of 4-nitrophenol. This work provides fundamental insights into sp-carbon reactivity while delivering practical platforms for multiple applications.
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COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2022
·
IF 9.463
Linlin Wang,Fenghua Zhang,Shanyi Du,Jinsong Leng
The cross-linking degree is a significant factor affecting the thermodynamic properties of resins, the cross-linking degree of photosensitive resins is limited during Digital Light Procession (DLP) printing. We present a strategy for double networks in epoxy resin to further improve the performances of printed structures via two different processing methods. Epoxy acrylates and polyethylene glycol dimethacrylates crosslink by UV-light radiation forming a light-induced network structure, and epoxy molecules crosslink under γ-ray radiation forming a ray-induced network structure. The two kinds of networks interpenetrate to form the interpenetrating polymer network (IPN). The printed IPN specimens have the characteristics of high Young’s modulus (up to 4.89 GPa) and fracture strain (as high as 30.4 %). The printed flowers and canister reinforced by short glass fibers (GFs) exhibited rapid shape recovery behaviors. The IPN epoxy/GFs composites have good values in manufacturing smart deployable structures , medical devices , and automobile shell parts.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Xianghe Liu,Bingqi Liu,Hongtao Xu,Haonan Li,Peng Li,Zhuo Zuo,Qibiao Wang
Efficient discrimination of thermal neutrons, fast neutrons, and γ-rays is of great importance for radiation monitoring, nuclear security, and fundamental research. However, conventional plastic scintillators, despite their low cost and fast response, generally suffer from limited neutron/gamma (n/γ) discrimination capability, which motivates the development of advanced scintillation materials and dedicated detection systems. In this work, we developed a polystyrene/poly(methyl methacrylate) (PS/PMMA) blended plastic scintillator that combines the high light yield of PS with the mechanical robustness of PMMA. A boron-containing dopant, bis(pinacolato)diboron (B2Pin2), was introduced together with optimized dye and cross-linker concentrations to enhance neutron sensitivity and overall scintillation performance. To evaluate the detector performance, we further constructed a custom measurement platform consisting of a photomultiplier tube (PMT)-based readout module and a high-speed pulse waveform analysis system. Experiments with a 241Am–Be source demonstrate that the developed scintillator exhibits effective pulse shape discrimination capability, demonstrating statistical discrimination of γ-ray-, fast-neutron-, and thermal-neutron-related event populations within a single detector system.
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