ADVANCED ENGINEERING MATERIALS
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2024
·
IF 3.4
Shuang Huang,Chuanjie Yao,Mengyi He,Xinshuo Huang,Zhengjie Liu,Jiayi Chen,Lelun Jiang,Hui-jiuan Chen,Xi Xie
Metabolic substances play a pivotal role in maintaining the body's regular physiological functions. When these processes are disrupted, it can lead to metabolic disorders which may cause severe damage to various organs. Diabetes mellitus, a prevalent metabolic disorder, arises from disturbances in sugar metabolism among other substances. Consequently, there's a pressing need to monitor metabolite levels for early diagnosis. To address this, in this study, a semi-implantable metabolite sensing system developed around functionalized microwires-based bundle electrodes (FMBE) is introduced. This FMBE device tracks glucose, hydrogen peroxide (H 2 O 2 ), and uric acid (UA) levels in vivo in real time, sensitively, and continuously. The FMBE is coated with Au nanoclusters and carbon nanotube–2D carbides and nitride (MXene) nanocomposites to enhance sensing surface area. In vitro characterizations affirm the FMBE's linear responsivity, detection sensitivity, and selectivity toward glucose, H 2 O 2 , and UA sensing. In addition, in in vivo testing in healthy and diabetic rats, it is demonstrated that FMBE is able to continuously monitor interstitial glucose, H 2 O 2 , and UA concentrations after implantation. The FMBE system, thus, stands out as a promising platform for real-time, in situ monitoring of metabolite concentrations, potentially assisting in the diagnosis of diabetes and associated complications.
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ACS Sensors
·
2024
·
IF 8.2
Fan Shi,Zejun Zhang,Behisht Ara,Zhitan Huang,Qifeng Gui,Ahmad Mansoor,Wei Sun
Fe single-atom and Fe cluster-coupled N, S co-doped carbon nanomaterials (FeSA–FeONC-NSC) were prepared through a two-step high-temperature pyrolysis process using Gelidium corneum enriched with C, Fe, O, N, and S as precursors. The analysis by aberration-corrected scanning transmission electron microscopy and X-ray absorption spectroscopy revealed the presence of single-atom Fe in Fe–N4 coordination structures, along with small clusters as Fe–O-coordinated Fe2O3. Single-atom Fe in the form of Fe2+/Fe3+ provides more electrocatalytic active sites, which synergistically accelerates the charge migration process in the assembly of FeSA–FeONC-NSC with Fe2O3 clusters. The flexible nonenzymatic sensor, based on FeSA–FeONC-NSC and fabricated using a polydimethylsiloxane substrate, exhibited excellent catalytic activity for both uric acid (UA) and tyrosine (Tyr). Low detection limits for UA (0.14 μmol L–1) and Tyr (0.03 μmol L–1) were observed by using chronoamperometry in artificial sweat. The in situ detection of sweat was performed in combination with an integrated circuit board affixed to human skin, and the results were generally consistent with those of the high-performance liquid chromatography method. Therefore, FeSA–FeONC-NSC serves as a good modifier for wearable electrochemical sweat sensor applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaoyang Zhu,Yang Zhang,Yu Zhang,Panpan Xie,Yunchuan Liu,Wenhui Zhang,Shenao Zhang,Yusen Wang,Guan Wu,Wangyang Lu,Nan Li
Customizing smart clothing for monitoring the physiological and vital sign information in real time has raised demands for wearable sensors with rapid response, good stability, high sensitivity and excellent mechanical properties. Herein, a flexible high-entropy doped oxide Cu 0.9 Fe 0.06 Ni 0.01 Cr 0.01 Co 0.01 Mn 0.01 (HE-Cu 2 O) nanoparticle modified Ti 3 C 2 T x (HECT) fiber was prepared through a facile hydrothermal growth and heat treatment process. Due to rich active sites, high reactivity and large surface area, HE-Cu 2 O appears impressive detection ability, admired adsorption inclination and exceptional reaction kinetics, confirming by DFT calculation. What’s more, the covalent coupling between the Ti 3 C 2 T x conductive frameworks and HE-Cu 2 O nanoparticles can further avoid the negative influence of metal oxide on the electronic conductivity, ensuring fast electron transfer rate. As a result, the HECT fiber can be operated in DA/PBS solution with reliable electrocatalyst properties, including low detection limit (1 μM), rapid response rate (<2 s), remarkable long-term cycling performance (100 cycling) and excellent reproducibility (RSD ≈ 2.01 %). Additionally, when the HECT fiber, matching with the Ag/AgCl electrode and Pt wire, weaves into the garment, the integrated system retains obvious response level, less response time (<5 s) and outstanding electrocatalyst performance under dynamic situations (bending, running, rope skipping and doing yoga), demonstrating a potential in practical application.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Jihong Zhou,Hongyu Chen,Xinyu Han,Ya Xu,Yuxi Meng,Miaomiao Tian,Taowen Dong,Fang Chai
As ascorbic acid (AA) plays a crucial role in the regulation of human redox metabolism, it is of great significance for reliable and sensitive detection AA. Considering this, heterogeneous flower-like bimetallic CuAg micro-composites (CuAg-MF) with unique oxidase-mimetic activity were fabricated to achieve the colorimetric sensing for AA. This strategy allows CuAg-MF to efficiently convert dissolved oxygen into •O 2 ¯ and catalyzes 3,3′,5,5′-tetramethylbenzidine (TMB) to generate an oxidized TMB (ox-TMB) with remarkable color changes, resulting in a colorimetric signal output. The detection of AA achieved by reducing ox-TMB in the obstruction of the chromogenic reaction, with a limit of detection (LOD) of 0.34 µM, and the performance stability, and reliability, as well as practicability were verified based on this mechanism. Notably, the integrating smartphone sensor provided portable, sensitive, rapid, and reliable detection of AA with a LOD of 0.39 µM, and excellent recoveries in environmental and food samples. This sensing platform was expected to apply as the efficient portable method and exhibit promising potential for AA monitoring in complex matrices.
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Tingting Wang,Fangning Liu,Chuanxia Chen,Yizhong Lu
Background Tyramine is an important index of food freshness degree, and tyrosinase that can specifically oxidized monophenolamine to catecholamine plays a crucial part in the occurrence and development of melanin-related skin diseases. Therefore, it is crucial to develop sensitive and efficient methods for the detection of tyramine and tyrosinase. Results In this work, encouraged by tyrosinase-triggered specific oxidation of tyramine to dopamine and the unique fluorescent reaction between dopamine and amino silane, we have developed a one-step synthetic strategy of silicon containing nanoparticles (Si CNPs) for “turn-on” detection of tyramine and tyrosinase. The Si CNPs formed with thoroughly studied mechanism exhibit uniform structure and robust yellow-green fluorescence. The low detection limits for tyramine (1.87 μM) and tyrosinase (0.0029 U/mL) demonstrate admirable sensitivity outstripping most methods. The proposed assay achieves satisfactory results in the determination of tyramine and tyrosinase activity in real samples. Furthermore, we leverage this new fluorescent assay to enable the fabrication of an “AND” Boolean logic gate. Significance The entire process can be completed at easily available temperature and pressure with rapid response, convenient operation and visual observation. This fluorescent assay featured with excellent sensitivity, selectivity and stability has considerable prospects in the application of biosensors and disease diagnosis.
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Microorganisms
·
2025
·Vol. 13
·期 4
·798
·
IF 4.2
Xiaoyu Cao,Yu Zhang,Qianqian Xu,Hai Yan
Hyperuricemia is a metabolic disorder owing to the underexcretion and/or overproduction of uric acid (UA). Recent studies have shown that probiotics have many potential applications as functional foods to ameliorate hyperuricemia. In this study, we have successfully isolatedBacillus paranthracisYC03 with urate-lowering potential from Jiangshui. The in vitro results indicated that YC03 exhibited strong biodegradation capacities toward UA and its precursors (inosine and guanosine). Meanwhile, the cell-free extracts of YC03 were also found to catalyze and remove inosine and guanosine. To further explore the application potential of this strain in developing functional foods, we evaluated its probiotic properties through in vitro assays and whole genome analysis.B.paranthracisYC03 has excellent abilities, with tolerance to acid and bile salt and good adhesion. In addition, hemolytic assays, along with antibiotic resistance and biogenic amine production tests, have also preliminarily confirmed the safety of using YC03 in food. We have also annotated the key enzyme genes, includingauaG,hpt,rih,punAanddeoD, which are involved in the biodegradation of UA and nucleosides. The results of nucleoside biodegradation product detection will be valuable for exploring the metabolic pathway for biodegrading nucleosides with YC03. These findings provide meaningful insights for the development of functional foods to improve hyperuricemia usingB.paranthracisYC03.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zhaoxian Chen,Shihong Liu,Wenrou Yu,Li Wang,Fengxue Lv,Liejun Yang,Huiqing Yu,Haiyang Shi,Yingzhou Huang
Comfortable treatment of malignant tumors is the clinical orientation of cancer therapy at present, which puts forward a high demand for non-invasive, portable and high-frequency monitoring status of tumor in the treatment. Unlike traditional blood and X-ray technique, here we have developed hydrogel-based wearable sweat sensors, equipped with multiple molecular receptors for surface enhanced Raman spectroscopy (SERS) monitoring treatment effects of lung cancer. The SERS technique was utilized in combination with multiple artificial intelligence (AI) algorithms (LGB GNB, LDA, RF, and SVM) to develop a novel and precise diagnostic model for monitoring the treatment effect. Based on 12617 SERS spectras from clinical patients, the results successfully diagnosed three treatment effects (progressive disease, partial response, and no change) with an accuracy of 89.7 %. Benefiting from data mining in AI algorithms, key Raman spectra features in clinical spectra are identified to explore characteristic biomarkers of lung cancer associated with various comorbidities. The clinical data suggest that carbonyl biomarkers in sweat might be crucial for understanding complications such as diabetes and hypertension. Our results not only offer a novel and comfortable monitoring technique but also enable personalized treatment of lung cancer with complications, presenting significant potential for clinical application.
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ACS Nano
·
2024
·
IF 15.8
Yingshuang Meng,Lingfeng Wang,Guangming Zhao,Jibo Diao,Zhibo Qi,Mingyang Yu,Zhonghua Li,Yuchao Niu,Gaohong He,Xiaobin Jiang
Gout flare-up, commonly resulting from monosodium urate monohydrate (MSUM) crystallization, has led to painful inflammatory arthritis among hundreds of millions of people. Herein, a kind of hydrogel nanoparticles (HNPs) with specific properties was developed, aimed at providing a promising pathway for MSUM crystallization control. The experimental and molecular dynamics simulation results synchronously indicate that the fabricated HNPs achieve efficient inhibition of MSUM crystallization governed by the mechanism of “host–guest interaction” even under very low-dose administration. HNPs as the host dispersed in the hyperuricemic model effectively lift the relative heterogeneous nucleation barrier of the MSUM crystal and hinder solute aggregation with strong electronegativity and hydrophobicity. The initial appearance of MSUM crystals was then delayed from 94 to 334 h. HNPs as the guest on the surface of the formed crystal can decelerate the growth rate by anchoring ions and occupying the active sites on the surface, and the terminal yield of the MSUM crystal declined to less than 1% of the control group. The good biocompatibility of HNPs (cell viability > 94%) renders it possible for future clinical applications. This study can guide the rational design of inhibitory nanomaterials and the development of their application in the control of relevant pathological crystallization.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Jiaxue Liang,Hang Qi,Feng Zhu,Shuqi Chen,Bohua Liu,Chongling Sun,Yanyan Wang
Real-time monitoring the drugs penetration into brain is crucial for clinical therapy of neurodegenerative diseases. In this study, a Blood-Brain Barrier-electrochemical sensing (BBB-ES) platform combining BBB on chip model and electrochemical sensing system was proposed, which facilitated stable BBB growth under fluidic conditions and realized real-time detection of L-Dopa across the BBB. Based on this system, the effects of fluid force on the cell morphology, skeleton arrangement and tight junction protein ZO-1 of BBB growth were evaluated, it was verified that the fluid force promote cell rearrangement. Additionally, L-Dopa detecting performance was optimized by modifying SWNTs-AuNPs-PPy/Tyr(Single-Walled Carbon Nanotubes-Au Nanoparticles-Polypyrrole/Tyrosinase) on electrochemical electrode, which showed a wide linear response range of 10–500 μM and excellent selectivity toward L-Dopa. The integrated BBB-ES system was finally applied to real-time monitor the penetration process of L-Dopa through the BBB, it was proved that the penetration of L-Dopa increased with the increase of flow rate and time, and finding that L-Dopa was delivered by passive diffusion without disrupting the integrity of BBB. Based on these findings, the system can be used to monitor the real-time process of different drugs penetrating BBB and even different organ barriers, which also provide the possibility to solve the clinical demand for drugs screen.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Zhuanzhuan Shi,Yunpeng Li,Xiaoshuai Wu,Bo Chen,Wei Sun,Chunxian Guo,Chang Ming Li
Point-of-care testing (POCT) has attracted great interest because of its prominent advantages of rapidness, precision, portability, and real-time monitoring, thus becoming a powerful biomedical device in early clinical diagnosis and convenient medical treatments. However, its complicated manufacturing process and high expense severely impede mass production and broad applications. Herein, an innovative but inexpensive integrated sandwich-paper three-dimensional (3D) cell sensing device is fabricated to in situ wirelessly detect H2O2 released from living cells. The paper-based electrochemical sensing device was constructed by a sealed sandwiched bottom plastic film/fiber paper/top hole-centered plastic film that was printed with patterned electrodes. A new (Fe, Mn)3(PO4)2/N-doped carbon nanorod was developed and immobilized on the sensing carbon electrode while cell culture solution filled the exposed fiber paper, allowing living cells to grow on the fiber paper surrounding the electrode. Due to the significantly shortening diffusion distance to access the sensing sites by such a unique device and a rationally tuned ratio of Fe2+/Mn2+, the device exhibits a fast response time (0.2 s), a low detection limit (0.4 μM), and a wide detection range (2–3200 μM). This work offers great promise for a low-cost and highly sensitive POCT device for practical clinic diagnosis and broad POCT biomedical applications.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-11
·
IF 5.3
Mei Lin,Shi Yanmei,Ding Xueke,Li Jing
Surface functionalization and the combined utilization of zero‐dimensional and two‐dimensional nanomaterials is an effective method to achieve highly sensitive detection for electrochemical analysis. Using an all-in-one strategy, phthalocyanine, gold nanoparticles, and ionic liquid were successively modified on the graphene surface as a highly integrated electrode modification material. Phthalocyanine can repair the defects of reduced graphene oxide by binding to the graphene structure surface through non-covalent functionalization. The combination of ionic liquid on the surface of gold nanoparticles can enhance their physical and chemical activity while preserving their stability. The obtained phthalocyanine-coated graphene nanosheets decorated with ionic liquid-functionalized gold nanoparticles nanocomposites had enhanced electrocatalysis and conductivity ability, and were used for highly sensitive electrochemical detection of ascorbic acid in fruit juices. Excellent results were obtained for the detection of ascorbic acid in the linear range 0.05 to 50 µmol/L with a detection limit of 6.80 nmol/L ( S/N = 3) and a sensitivity of 2.68 μA μM −1 cm −2 , indicated that the proposed sensor strategy with multiple signal amplification can achieve higher detection sensitivity. Furthermore, the sensor was used to quantify ascorbic acid content in grapefruit and orange juice with good selectivity and accuracy. The highly integrated electroactive nanocomposites construction method described may also be used with other electrode modification materials and is anticipated to yield fresh perspectives on the advancement of ultrasensitive detection. Graphical
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Fangbing Wang,Jing Ren,Qiwen Peng,Hongyi Sun,Qiankun Zeng,Yongheng Zhang,Guoyue Shi,Min Zhang
Plasma separation, rich in biomarkers crucial for diagnosis, is conventionally achieved via high-speed centrifugation, a method hindered by its blood usage, lengthy processes, and complex operations, which delays detection. We introduced a novel real-time blood sensing method based on a Janus membrane and enzymes @MOFs. Asymmetric driving of the janus membrane can realize spontaneous separation of plasma and prevent hemolysis during direct separation. Glucose oxidase (GOx), uric acid oxidase (UOx) and horseradish peroxidase (HRP) were encapsulated in a hydrophilic organometallic framework (MOFs) to construct an enzyme cascade nanoreactor. Embedding enzyme in hydrophilic MOFs not only retains the natural conformation of free enzyme but also improves the brittleness of enzyme, endows MOFs with new biological functions, and expands its sensing application. Using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogen and a custom app for color interpretation, we achieved real-time visualization of glucose (Glu) and uric acid (UA) at a 50 μM limit. The system accurately analyzed serum samples, matching commercial kits and showing promise for portable, personalized diagnostics.
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