JOURNAL OF MEMBRANE SCIENCE
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2025
·
IF 9
Mei-Ling Liu,Ying Tang,Lu Li,Yuanyuan Rao,Shi-Peng Sun,Weihong Xing
The adhesion of bacteria from aquatic environments onto membrane surfaces can severely compromise the membrane’s fluorinated compound rejection efficiency. While graphene oxide membranes demonstrate potential for pollutant separation, their practical application is constrained by interlayer swelling and interfacial instability. This study developed a reduced graphene oxide (rGO) composite membrane cross-linked via supramolecular host-guest interactions and ionic synergy, using cucurbit[6]uril (CB6) and silver ions, supported on polyaniline/polyimide electrospun nanofibers. CB6 formed stable complexes through ion-dipole interactions of polar carbonyl groups with silver ions, while cation-π interactions of silver ions with rGO establish a ternary cross-linked network, effectively suppressing interlayer swelling and precisely tuning nanochannel size. The membrane achieves a 98.2% rejection rate for perfluorooctanoic acid. Additionally, silver ions, CB6, and rGO endow the membrane with long-lasting antibacterial properties, exhibiting a 99.6% inhibition rate against Escherichia coli . This supramolecular synergistic strategy offers a novel approach for designing multifunctional membranes with high-efficiency separation and anti-biofouling performance.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Qiuhui Qian,Ji Wu,Cuizhen Wang,Zheng Yang,Ying Cheng,Yuansi Zheng,Xuedong Wang,Huili Wang
As a synthetic rubber antioxidant, the environmental monitoring concentrations of N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6-PPD) have exceeded the risk threshold, attracting widespread attention. Although investigations into the harmful effects on zebrafish have commenced, a comprehensive exploration of its toxicological impacts and underlying molecular mechanisms remains to be conducted. By using zebrafish as a model, this study systematically evaluated 6-PPD-induced lipid metabolism disorders and inflammation response following environmental exposure. Bioinformatics analysis revealed that 6-PPD target genes enriched in the hepatitis B pathway, indicating potential hepatic toxicity via inflammatory pathways. Therefore, we hypothesize that 6-PPD could trigger hepatotoxicity through the crosstalk between lipid metabolism and inflammation. Further experiments substantiated this hypothesis by showing lipid accumulation in the liver following 6-PPD exposure, along with elevated triglyceride (TG) and total cholesterol (TC) levels, and imbalanced expression of lipid metabolism-related marker genes. Additionally, 6-PPD exposure induced the accumulation of reactive oxygen species (ROS) and inhibited the differentiation and maturation of immune cells, resulting in immune evasion. Most of these abnormalities were exacerbated in a dose-dependent manner with increasing concentrations of 6-PPD. The addition of the PPARγ pathway agonist puerarin (PUE) or NF-κB pathway inhibitor quinazoline (QNZ) to 6-PPD exposure group mitigated these toxic effects, validating our conjecture that lipid metabolism disorder and inflammatory responses may result from the regulation of the PPARγ/NF-κB pathway.
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-10
·
IF 5.3
Chen Fei,Wang Xiang,Yan Lei,Lin Zhixiong,Zheng Weijun,Wu Dianming,Qiu Bin,Fang Yifan,Liu Mingkun
By preparing gold nanoparticles-graphdiyne (Au-GDY) as sensing substrate and [Ru(NH 3 ) 6 ] 3+ -UiO-66-NH 2 MOFs (Ru-UiO-66) as signaling probe as well as linking the as-prepared Au-GDY and Ru-UiO-66 with the specific peptide (PT) of MMP-7 to form Ru-UiO-66/PT/Au-GDY, a cost-effective and sensitive electrochemical sensor was designed for the detection of matrix metalloproteinase-7 (MMP-7) which is an important biomarker for tumor and biliary atresia. The superior electrical properties, easy preparation, large surface area, and high stability of Au-GDY make it be a desirable substrate to immobilize PT. The unique structure of UiO-66-NH 2 is beneficial to assemble [Ru(NH 3 ) 6 ] 3+ probe and link PT. In the absence of MMP-7, the Ru-UiO-66/PT/Au-GDY modified electrode exhibited a prominent peak current of [Ru(NH 3 ) 6 ] 3+ , which is decreased after the addition of target MMP-7 since it can specifically recognize and cleave PT to induce the Ru-UiO-66 leave the electrode. The experimental results showed that the decrease of the [Ru(NH 3 ) 6 ] 3+ signal has a linear relation with the concentrations of MMP-7 in the range 0.01 pg mL −1 –10.0 ng mL −1 . By optimizing experimental conditions, the achieved detection limit (LOD) for MMP-7 is 5.0 fg mL −1 . The analysis of real serum samples has excellent accuracy. The simplicity of preparation and sensitivity make the Ru-UiO-66/PT/Au-GDY sensor a considerable promising analysis tool for MMP-7 detection. Graphical abstract
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高影响因子
JOURNAL OF GENE MEDICINE
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2020
·Vol. 22
·期 9
·e3201
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IF 3.258
Liping Ma,Yanjing Cao,Lin Zhang,Ketao Li,Laixing Yan,Yizhan Pan,Jianhua Zhu
Background Celastrol (Cel) has been corroborated as an anti-inflammatory and anti-apoptotic agent in multiple cell damage models. However, the protective effect of Cel in high glucose (HG)-induced cardiomyocyte injury is still unclear. The present study aimed to determine whether Cel can mitigate HG-stimulated cardiomyocyte injury via regulating the miR-345-5p/growth arrest-specific 6 (Gas6) signaling pathway. Methods Cardiomyocytes were exposed to normal glucose (NG; 5 mmol/l) or HG (30 mmol/l) and then administered with Cel. Cell counting kit-8 and flow cytometry assays were used to detect cell proliferative activity and apoptosis. mRNA and protein expression were analyzed using a quantitative reverse transcriptase-polymerase chain reaction and western blotting, respectively. A bioinformatics algorithm and a luciferase reporter gene assay were used to determine whether Gas6 is a direct target of miR-345-5p. Results The present study confirmed the inhibitory effects of Cel in HG-induced inflammation in cardiomyocytes. Moreover, Cel exhibited the ability to antagonize HG-induced cardiomyocyte apoptosis and suppress the elevated Bax/Bcl-2 ratio elicited by HG stimulation. Intriguingly, Cel treatment revoked the HG-triggered repression of Gas6 protein expression, and Gas6 loss-of-function accelerated HG-induced cardiomyocyte apoptosis. HG-triggered up-regulation of miR-345-5p expression was depressed following Cel treatment. Importantly, we validated that Gas6 is a direct target of miR-345-5p. Transfection with miR-345-5p inhibitors restrained HG-induced release of pro-inflammatory cytokines and cell apoptosis. Conclusions The findings of the present study demonstrate that Cel administration antagonized HG-induced cardiomyocyte apoptosis and inflammation through up-regulating Gas6 expression by restraining miR-345-5p.
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PubMed
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2025
·
IF 3.1
Jiahui Sun,Luyao Shi,Fan Xu,Hanyan Sun,Yitong Liu,Jiangyun Sun,Qingxin Zhou
Colorectal cancer (CRC) is a worldwide health issue. It causes illness and death in millions of people each year. A positive correlation has been observed between the intake of dietary fat and the development of CRC. The composition of gut microbiota exhibits a significant correlation with pathophysiologic processes in intestine. Clinical treatment remains inadequate due to the complex pathogenic mechanisms of CRC triggered by a high-fat diet (HFD). Naringenin, a flavonoid from grapefruit, has anti-cancer activity. Our findings suggest that naringenin enhances gut microbiota diversity by increasing the abundance of beneficial bacterial species while reducing opportunistic pathogenic bacteria. The fecal microbiota transplantation assay (FMT) demonstrated that the anti-HFD-CRC activity of naringenin depended on the gut microbiota. Furthermore, naringenin antagonized the IL-6/STAT3 pathway. These results suggest that naringenin may be a potential treatment for HFD-CRC.
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PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 11
·期 2
·157-162
·
IF 2.073
Jimin Yang,Xuesong Yin,Wei Zhang
In our study, we report the development of molybdenum disulfide (MoS2) nanosheet-supported poly(indole-6-carboxylic acid) (PIn6COOH) and its usage for the electrochemical determination of circulating tumor DNA (ctDNA) such as PIK3CA gene associated with breast cancer. The MoS2 nanosheets prepared by the solvent exfoliation method from commercial bulk MoS2 were first immobilized on the surface of a carbon paste electrode; then, PIn6COOH, a novel electroactive material, was electropolymerized on the MoS2 substrate by the potentiostatic method to form a unique nanocomposite structure. The physical adsorption between aromatic In6COOH monomers and MoS2 efficiently improved the electropolymerization efficiency, resulting in increased electrochemical response of PIn6COOH. Owing to the presence of abundant carboxyl groups, probe ssDNA was covalently immobilized on the carboxyl-terminated PIn6COOH/MoS2 nanocomposite through free amines of DNA sequences based on 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydrosulfosuccinimide crosslinking reaction. The covalently immobilized probe ssDNA could selectively hybridize with its target DNA to form dsDNA on the surface of the PIn6COOH/MoS2 nanocomposite. The self-signal changes of PIn6COOH induced by DNA immobilization and hybridization could be sensitively recognized via cyclic voltammetry and electrochemical impedance spectroscopy. This developed biosensor showed high performance of DNA hybridization with a detection limit of 1.5 × 10−17 mol L−1 for the detection of PIK3CA gene. The easy construction of this biosensor besides its fine sensitivity and very low time consumption can make this PIn6COOH/MoS2 nanocomposite very highly competitive for further applications in carcinoma diagnostic and therapeutic fields.
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高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
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2020
·
IF 3.347
Yang Zhang,Jiabei Zhou,Qin Ye,Kui Zeng,Jie Pan,Lu Chen,Yingying Wang,Bo Yang,Qiaojun He,Jianqing Gao,Su Zeng,Lushan Yu
Thioredoxin 1 (Trx1) and telomerase play key roles in the development and progression process of most tumors, and they both are promising drug therapy targets. We have, for the first time, discovered that Trx1 and telomerase had a dual-target synergistic effect. Based on that results, we designed a series of 6-dithio-2′-deoxyguanosine analogs (named as YLS00X) and verified whether they can inhibit Trx1 and telomerase simultaneously. TrxR1/Trx1 system activity and telomerase expression were significantly inhibited by 6-dithio-2′-deoxyguanosine analogs, especially YLS004. YLS004 can also cause ROS accumulation, and induce tumor cell apoptosis . The vitro antitumor activity of 6-dithio-2′-deoxyguanosine analogs using MTT assay on 11 different human cancer cells and found that human colon cancer cells(HCT116) and melanoma cells (A375) were the most sensitive cells to 6-dithio-2′-deoxyguanosine analogs treatment and vivo xenografts models also confirmed that. The serum biochemical parameters and multiple organs HE staining results of subacute experiments indicated that YLS004 might be mildly toxic to immune organs, including the thymus, spleen, and hematopoietic system . Besides, YLS004 was rapidly metabolized in the rats' blood. Our study revealed that YLS004, a Trx1 and telomerase inhibitor , has strong anti-tumor effects to colon cancer and melanoma cells and is a promising new candidate drug.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
·
2021
·Vol. 135
·期 2
·431-440
·
IF 2.686
Jiang Bowei,He Wei,Jin Chuan,Liu Ying,Wen Dan,Wang Chudong,ZEYE Moutanou Modeste Judes,Li Jienan,Zha Lagabaiyila
Genotyping of short tandem repeat (STR) markers is the basic method of forensic science. Enhanced technologies are needed to meet the requirements of databasing and casework samples. The STRscan-17LC kit is a 6 Dye STR kit which amplifies 16 STR loci: D3S1358, TPOX, D16S539, vWA, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, D18S51, TH01, D12S391, and D21S11 and the sex-determinant locus amelogenin. This kit is designed for better tolerance to PCR inhibitors and analysis of mildly degraded samples with all fragments smaller than 330 bases. In this study, the STRscan-17LC kit is validated according to the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines, including PCR-based studies, sensitivity, precision and accuracy, inhibitors, species specificity, DNA mixture studies, population, and concordance studies. The validation results suggest that the STRscan-17LC kit is a useful tool for forensic application.
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PubMed
高影响因子
Cell Reports
·
2022
·
IF 9.995
Xinyu Li,Jiasheng Li,Di Zhu,Ning Zhang,Xusheng Hao,Wenfeng Zhang,Qian Zhang,Yangli Liu,Xueying Wu,Ye Tian
Summary Coordination of inter-tissue stress signaling is essential for organismal fitness. Neuronal mitochondrial perturbations activate the mitochondrial unfolded-protein response (UPR mt ) in the intestine via the mitokine Wnt signaling in Caenorhabditis elegans . Here, we found that the protein disulfide isomerase PDI-6 coordinates inter-tissue UPR mt signaling via regulating the Wnt ligand EGL-20. PDI-6 is expressed in the endoplasmic reticulum (ER) and interacts with EGL-20 through disulfide bonds that are essential for EGL-20 stability and secretion. pdi-6 deficiency results in misfolded EGL-20, which leads to its degradation via ER-associated protein degradation (ERAD) machinery. Expression of PDI-6 declines drastically with aging, and animals with pdi-6 deficiency have decreased lifespan. Overexpression of PDI-6 is sufficient to maintain Wnt/EGL-20 protein levels during aging, activating the UPR mt , and significantly extending lifespan in a Wnt- and UPR mt -dependent manner. Our study reveals that protein disulfide isomerase facilitates Wnt secretion to coordinate the inter-tissue UPR mt signaling and organismal aging.
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PubMed
高影响因子
ADSORPTION SCIENCE & TECHNOLOGY
·
2015
·
IF 0.669
Ping Yu,Qilong Sun,Zhenjiang Tan,Minjia Meng,Jianming Pan,Yongsheng Yan,Chunxiang Li
Highly effective magnetic molecularly imprinted polymer (MMIP) beads for adsorption of 2,4,6-trichlorophenol (2,4,6-TCP) were synthesized by suspension polymerization. The effect of various parameters such as bed depths (0.6, 1.1 and 1.7 cm), flow rate (1, 2 and 3 ml minute−1), influent 2,4,6-TCP concentrations (50, 100 and 200 mg l−1), influent solution pH (4, 6 and 8) and temperature (288, 298 and 308 K) was investigated. The breakthrough time increased with the increase of the bed depth, but decreased with an increase of the initial concentration, flow rate and temperature. The Adams–Bohart and Thomas models were applied to the adsorption process under experimental conditions to predict the breakthrough curves for process design. The Thomas model was in good agreement with the experimental data. Compared with magnetic non-imprinted polymers, MMIPs exhibited an excellent selectivity towards 2,4,6-TCP.
研究领域:
环境科学
·
材料科学
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高影响因子
Desalination and Water Treatment
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2016
·
IF 1.272
Quan Zhang,Fei-yun Sun,Wen-yi Dong,Ru-bing Han,Lei Liu
A laboratory-scale flat-sheet membrane bioreactor (MBR) was employed to treat real micro-polluted surface water loaded with 2,4,6-trichlorophenol (2,4,6-TCP). MBR treatment performances were evaluated in terms of dissolved organic matters (DOM) degradation, target trace organic (2,4,6-TCP) removal, and effluent bio-stability improvement. The experimental results showed that the removal efficiency of DOM related closely with the biomass content in MBR. With continuous growth and accumulation of biomass under a long sludge retention time (SRT), stable DOM removal efficiency above 50% could be obtained, resulted in an effluent DOM kept below 2.0 mg TOC/L. Upon the steady state of MBR, more than 90% 2,4,6-TCP was removed by synergistic function of aeration volatilization, biological degradation, and activated sludge adsorption. The removal pathways of 2,4,6-TCP were specified by series of batch-scale trials, and corresponding contributions were estimated. It was revealed that fresh ultrafiltration rejection took a major responsible for 2,4,6-TCP removal in the start-up phase, which rejected about 15% 2,4,6-TCP. In comparison, for a steady state where had a high biomass content around 450 mg/L, biodegradation was the main pathway for 2,4,6-TCP removal, reflected by that above 300 μg/L 2,4,6-TCP was eliminated by microorganism degradation within no more than 1.2 h. The results herein claimed that MBR is a feasible way to treat micro-polluted water resource, and its removal pathway specification gave reliable guidance for MBR application.
研究领域:
环境工程
·
水处理技术
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高影响因子
Chemical Engineering Journal Advances
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2020
·
IF 0
GAO Shan,WANG Li,ZHANG Zhijia,LI Rui,WANG Qiang,WANG Guojun,WEI Hao,OUYANG Xiao
Visual and selective detection of 2,4,6-trinitrophenol (TNP) for public and environmental protection is an extremely urgent issue and challenge. Herein, a novel fluorescence polyurethane (FPU) has been successfully synthesized and exploited for selective TNP-detection in coexistence with other commonly competitive nitroaromatic analytes. Excellent recognition ability of FPU toward TNP could reach as low as 0.12 μM and the observed fluorescence responses of FPU are exceptional stable to acid, alkali, and salt. Furthermore, a portable, cost effective, and simple indicator paper depending on FPU for visual detection of TNP is developed. FPU indicator paper exhibits two unique advantages: one is the higher selectivity for TNP detection, the other is realizing the rapid and visual explosives detection. The detection mechanism is described for the synergies in the photoelectron transfer (PET), the fluorescence internal filtration effect (IFE) and the fluorescence energy transfer (FRET). This work provides a novel guidance for the design of fluorescent polymers with portable, cost effective, and visual detection of TNP.
研究领域:
环境科学
·
分析化学
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