Advanced Science
·
2025
·
IF 14.1
Jie Zhao,Xinyi Liu,Dan Li,Xuefei Jing,Weijie Wu,Liangrui He,Yizhang Tang,Zhen Liu,Yulin Zeng,Haitian Sha,Huibin Qiu,Xujiang Yu,Wanwan Li
Constructing colloidal particles into designated hierarchies with integrated functionalities offers insights into bottom-up material fabrication. However, the arbitrary and controllable assembly of individual nanoscale and microscale particles remains highly challenging due to their diverse structural properties. Here, a general paradigm for the robust co-assembly of plasmonic noble metal nanoparticles and multifunctional polymer microspheres through thermodynamically driven heterocoagulation and coordination interactions is developed. Monodisperse poly(styrene-co-maleic anhydride) microspheres (PSMA MSs) are facilely synthesized using a one-step emulsification method with optional simultaneous encapsulation of magnetic and fluorescent nanoparticles. Upon surfactant removal, the purified PSMA MSs become metastable and trigger ultrafast co-assembly of silver nanocubes (AgNCs) onto MSs within 5 min with the maximum coverage rate reaching nearly 50%. The densely immobilized AgNCs outside magnetic MSs exhibit excellent catalytic efficiency (>89% conversion across 15 cycles) and enable enzyme integration for cascade reactions. Moreover, the largest reported plasmonic-fluorescent encoded array (124 codes) is realized, achieving a significantly improved detection limit of 10 copies/reaction for respiratory viruses. This work addresses the long-standing challenge of achieving uniform distribution and dense immobilization of plasmonic noble metal nanoparticles and offers a general approach to fabricating designated hierarchies based on noble metal nanoparticles, with integrated functionalities for high-performance practical applications.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Nei Wu,Yuling Yang,Jianming Xu,Haizhen Wang
Heavy-metal-tolerant bacteria provide a sustainable, eco-friendly approach to remediate cadmium (Cd) contaminated soils, and elucidating their tolerance mechanisms is key for designing effective bio-remediation strategies. This study integrated whole-genome sequencing, untargeted metabolomics and multi-phenotypic analyses to reveal the Cd tolerance mechanisms and soil remediation effects of three plant-growth-promoting bacteria: Rhizobium sp. WW22, Burkholderia sp. WW13 and Pseudomonas sp. WW2. Results showed that three strains achieved Cd adsorption efficiencies of 60.27 %-99.56 % in 0.3–100 mg L-1 Cd solutions, with amino, carboxyl, hydroxyl, and amide groups on cell surfaces as major binding sites. They have evolved gene clusters related to metal transport/efflux and oxidative stress response, which synergistically maintain metabolic homeostasis under Cd stress. Among them, strain WW13 exhibited stronger Cd tolerance due to abundant unique gene clusters for antioxidant defense, siderophore synthesis, and putrescine metabolism. Notably, three strains showed convergent metabolic responses under Cd stress, with secretions (predominantly organic oxygen compounds, lipids/lipid-like molecules, benzenoids, and organoheterocyclic compounds) that significantly increased environmental pH. Key common upregulated secretions (itaconic acid, cis/trans-aconitic acid) increased soil pH by 0.01–0.32 units and reduced the proportion of exchangeable Cd by 2.22 %-9.19 % within 10 days. Pot experiments further confirmed that strains indirectly reduced Cd bioavailability by increasing soil pH, enriching hydroxyl/carboxyl/amino groups, regulating dissolved organic carbon/nitrogen, ammonium nitrogen, activities of β-glucosidase and N-acetyl-β- D -glucosaminidase. By day 14, the proportion of exchangeable Cd was reduced by 4.09 %-5.86 %. This study revealed multi-pathway Cd tolerance mechanisms in bacteria and their roles in soil Cd immobilization, providing theoretical basis and efficient strains for bioremediation of Cd-contaminated soils.
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高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 35
·5693-5700
·
IF 5.342
Taijun He,Zhenyu Xing,Yixing Wang,Difeng Wu,Yang Liu,Xiangyang Liu
Generally, surface initiated-atom transfer radical polymerization (SI-ATRP) often suffers from the immobilization of the initiator on the surface-inert substrate. Developing an effective and versatile one-step method to anchor the priming sites while retaining the bulk properties of the substrate still faces challenges. In this work, direct fluorination is proposed to treat the benzene ring-containing substrate and the C–F introduced on the benzene ring can be used as an initiation site to achieve SI-ATRP. Computer and molecular simulation experiments have shown that the C–F added on the phenyl ring is more suitable for initiating ATRP than the C–F on the alkane chain. Thereafter, polyethylene terephthalate (PET) fabric was successfully grafted with fluorinated methacrylate polymer via direct fluorination-initiated ATRP, resulting in durable superhydrophobic properties, which demonstrated the practicability of the method. Furthermore, fluorination-initiated ATRP presents universality with other phenyl ring-containing polymeric substrates, such as aramid fiber, polyimide fiber and poly-p-phenylene benzobisoxazole fiber. Among them, the fluorinated aramid fiber can be grafted with hydroxyethyl methacrylate to obtain increased interfacial shear strength.
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高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 7
·4179-4189
·
IF 5.044
Zhang Shaobo,Zhou Yuhang,Zhu Gaolu,Jiang Yongze,Xie Jiulong,Qi Jinqiu,Yang Hanbo,Hui Wenkai,Huang Jintian
Abstract The magnetic properties of the magnetic transparent composite films largely depend on the content of Fe 3 O 4 employed. However, higher addition of Fe 3 O 4 into the matrix will lead to Fe 3 O 4 nanoparticles agglomeration resulting in the reduction in the transparency of the composite films. The contradiction between Fe 3 O 4 nanoparticle content and transparency limits the application of transparent magnetic films. In this work, the spherical bamboo nanocellulose (SBN) with an average size of 61 nm was prepared from bamboo powder by NaOH/Urea/Thiourea aqueous system, which was an effective, inexpensive and less toxic strategy. The basic structure of SBN was investigated. The SBN exhibited the cellulose II type crystal structure. The molecular chain length of SBN were lower than before dissolution. The magnetic SBN/Fe 3 O 4 composite films with light penetration were prepared. The results revealed that the dispersion of Fe 3 O 4 nanoparticles in the SBN/Fe 3 O 4 composite films was much better than that in nanofibrillated cellulose (NFC)/Fe 3 O 4 composite films because of the abundant hydrogen bonds between Fe 3 O 4 nanoparticles and SBN. The transparent results indicated the highest transmittance of the films was 94% in the wavelength range of visible light, which was almost 23.7% higher than that of NFC/Fe 3 O 4 composite films with the same amount of Fe 3 O 4 addition (15 wt%). The findings in this study also revealed that the SBN in the SBN/Fe 3 O 4 composite films was distributed in layers. The saturation magnetization of the SBN/Fe 3 O 4 composite films was 10.65 emu g −1 with 15 wt% Fe 3 O 4 incorporated, which guaranteed the excellent magnetic properties. Graphic abstract
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Xing Cao,Qiushi Zhang,Weichen Yang,Lin Fang,Shiwei Liu,Rui Ma,Kai Guo,Ning Ma
The high chlorine (Cl) and lead (Pb) content characteristics of municipal solid waste incineration fly ash (MSWIFA) pose environmental risks and hinder resource utilization. Herein, an MSWIFA-based magnesium potassium phosphate cement (MKPC) preparation strategy was developed, which allowed the MSWIFA recycling and the Pb-Cl synergistic immobilization without the washing pretreatment . The compressive strength of the resulting 10 wt% MSWIFA-based MKPC was 28.44 MPa, with over 99.2% reduction in leaching toxicity of Pb and Cl. The high-angle annular dark field scanning transmission electron microscope (HAADF-STEM) and X-ray absorption spectroscopy (XAS) analyzes showed that Pb, phosphate and Cl - formed Pb 5 (PO 4 ) 3 Cl in MKPC. In-situ X-ray diffraction (XRD) tests showed that Pb 3 (PO 4 ) 2 was gradually transformed to Pb 5 (PO 4 ) 3 Cl through a dissolution-precipitation process. The formation energy, Bader charge, charge density difference and density of states (DOS) of Pb 5 (PO 4 ) 3 Cl were analyzed by first-principles calculations, confirming that Pb 5 (PO 4 ) 3 Cl was more thermodynamically stable than Pb 3 (PO 4 ) 2 and PbCl 2 and that electronic interactions between Pb-p, O-p, P-p and Cl-p orbits were the origin of Pb-Cl synergistic immobilization. This work provides a new strategy for the resource utilization of MSWIFA without washing pretreatment, and provides an in-depth understanding of the Pb-Cl synergistic immobilization mechanism.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 2.892
Yinzhou Guo,Jiangtao Liu,Kehu Zhang,Honghong Zhang,Yan Li,Zhongli Lei
In this study, a novel type of stimuli-responsive support materials was synthesized successfully by atom transfer radical polymerization (ATRP) and ring opening polymerization (ROP) of N-isopropylacrylamide (NIPAAm), tert -butyl methacrylate (tBMA) and ε-caprolactone (CL). β-Cyclodextrin (β-CD) and azobenzene (Azo) were employed as the starting groups to form PCL-hv-PNIPAAm by host–guest inclusion complexation, while PCL-PMAA was converted by simple hydrolysis of PCL-PtBMA with trifluoroacetic acid. Both PCL-PMAA and PCL-hv-PNIPAAm formed the microspheres by self-assembly. Their characteristics were confirmed by NMR, TEM, GPC, DLS, etc. Then, the microspheres were used for the immobilization of pectinase by electrostatic interaction. The optimal immobilization reaction parameters were 10 U/mL for pectinase at 25 °C for 4 h at pH = 6. Compared with free pectinase, the immobilized enzymes were found to exhibit better tolerance of variations in pH and temperature, as well as improved storage stability. Furthermore, the relative activity of the immobilized pectinase was controlled at approximately 13% when the temperature was higher than the LCST of PNIPAAm. With different light irradiation, there is approximately a 15% influence on the relative activity of the immobilized pectinase. This new enzyme support material has good environmental adaptability and can make the activity of immobilized enzymes be tailored predictably and enable the enzyme to be used as a biocatalyst for various applications in the food industry.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiang Qiu,Shushu Wang,Shanshan Miao,Hongbo Suo,Huajin Xu,Yi Hu
This work aims to achieve the co-immobilization of laccase and 2,2-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) to improve removal capability of the biocatalyst for pollutants while avoiding potential pollution caused by ABTS. The laccase was immobilized on magnetic chitosan nanoparticles modified with amino-functionalized ionic liquid containing ABTS (MACS-NIL) based on Cu ion chelation (MACS-NIL-Cu-lac). The carrier was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, x-ray diffraction and etc., and electron paramagnetic resonance confirmed the mediator molecule ABTS on the carrier could also play the role of electron transmission. MACS-NIL-Cu-lac presented relatively high immobilization capacity, enhanced activity (1.7-fold that of free laccase), improved pH and temperature adaptability, and increased thermal and storage stability. The removal performance assay found that MACS-NIL-Cu-lac had a good removal efficiency with 100.0 % for 2,4-dichlorophenol in water at 25 °C, even when the concentration reached 50 mg/L. Reusability study showed that after six catalytic runs, the removal efficiency of 2,4-dichlorophenol by MACS-NIL-Cu-lac could still reach 93.2 %. Additionally, MACS-NIL-Cu-lac exhibited higher catalytic efficiencies with 100.0 %, 70.5 % and 93.3 % for bisphenol A, indole, and anthracene, respectively. The high catalytic performance in pure water system obtained by the novel biocatalyst co-immobilizing laccase and electron mediator ABTS showed greater practical application value.
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高影响因子
TALANTA
·
2016
·
IF 4.035
Yun Guan,Lanjunzi Liu,Chao Chen,Xiuzhi Kang,Qingji Xie
The facile preparation of poly( l -DOPA)-tyrosinase (PD M -Tyr) composite and its application both in substrate (phenol) and inhibitor (atrazine) sensing is reported here for the first time. Effective immobilization of enzyme is realized via in-situ entrapping Tyr in poly( l -DOPA) (PD M ), which is formed by Tyr catalytic polymerization of l -DOPA. The Tyr modified electrode is simply prepared by dipping the PD M -Tyr composite on an Au electrode and then covered by Nafion. The thus-prepared Tyr-immobilized electrode exhibits excellent performance superior to most Tyr-based electrochemical biosensors, the sensitivity to phenol is as high as 5122 μA mM −1 in the linear range of 10 nM~1.25 μM, the apparent Michaelis-Menten constant ( K M app ) determined as low as 3.13 μM indicates strong substrate binding and high catalytic activity of the immobilized Tyr. The biosensor also works well in atrazine biosensing, with a linear detection range of 50 ppb~30 ppm and a low detection limit of 10 ppb obtained. In addition, the biosensor shows excellent stability, precision, high sensitivity and fabrication simplicity.
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高影响因子
Bioresource Technology Reports
·
2021
·
IF 0
Ya Liu,Rui Pei,Zhongliang Huang,Jingran Xiao,Ayan Yao,Kaiji Xu,Yixin Li,Shafqat Ullah,Zongzhong Yu,Yuanpeng Wang,Shu-Feng Zhou,Guowu Zhan
Herein, we propose a facile synthetic strategy for biogenic photocatalyst by sequentially immobilizing CdS and Pt nanoparticles (NPs) on engineered recombinant Escherichia coli ( e-E. coli ) strains. In particular, we transferred the cysteine desulfurase gene to the heterologous host to overexpress cysteine desulfurase and the resulting e-E. coli strain could serve as a biofactory for the extracellular growth of CdS NPs involving catalyzing L-cysteine (Cys) to form a reactive sulfur source, followed by the precipitation of CdS via the reaction between Cd 2+ ions and the locally concentrated active sulfur source around the strain. The subsequent modification of e-E. coli /CdS composites with noble metal Pt NPs further enhanced the catalytic degradation performance towards organic dyes, due to suppressing the recombination of the photogenerated electron-hole pairs. Accordingly, the ternary e-E. coli /CdS/Pt composites emerged as robust photocatalysts for pollutant degradation in waste-water treatment, which is promising for environmental remediation in ambient conditions.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Minghui Xiong,Bo Chai,Guozhi Fan,Xiaohu Zhang,Chunlei Wang,Guangsen Song
The strong electron interaction between metal oxide–carbon-based catalyst components plays a vital role in the peroxymonosulfate (PMS) activation for pollutant degradation. Herein, a novel CoOOH nanosheets anchored on rape straw-derived biochar (BC) surface (labeled as CoOOH/BC) as an efficient PMS activator toward degrading sulfamethoxazole (SMX) was synthesized. Experimental results indicated that integrating CoOOH nanosheets on the BC surface could inhibit CoOOH aggregation to increase the specific surface areas, exert a component synergistic effect to enhance activation degradation activity, and improve the catalyst stability. As a result, a 96 % degradation efficiency of SMX was achieved within 20 min over 20 wt% CoOOH/BC composite catalyst under the optimal conditions. Density functional theory (DFT) calculations disclosed that a built-in electric field (BIEF) pointing from BC to CoOOH was constructed at their interface, which could mediate PMS activation for reactive oxygen species (ROS) generation and induce direct electron transfer from SMX to PMS, resulting in efficient SMX degradation via both radical and non-radical pathways. Moreover, quenching experiments and electron paramagnetic resonance (EPR) measurements confirmed that single oxide ( 1 O 2 ) and superoxide radical ( O 2 · - ) are the dominant active species in the current system. Additionally, the possible SMX degradation routes were reasonably proposed based on liquid chromatography-mass spectrometry (LC-MS) results. This work provides an in-depth understanding of the role of BIEF in PMS activation, and expands the application of biochar-based materials in the field of environmental remediation.
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高影响因子
PLASMA CHEMISTRY AND PLASMA PROCESSING
·
2011
·Vol. 31
·期 6
·811-825
·
IF 1.798
Wei Yanlin,Chen Yashao,Liu Peng,Gao Qiang,Sun Yu,Huang Changzheng
To improve application of hydrophobic polymethyl methacrylate intraocular lens (PMMA IOL) in a convenient and continuous way, 2-hydroxyethyl methacrylate (HEMA) was immobilized by dielectric barrier discharge plasma at relatively high pressure. The hydrophilicity and topography of the modified IOL surface were comprehensively evaluated by contact angle and atomic force microscopy, while the surface biocompatibility of the modified IOL was investigated by platelets adhesion and cells proliferation experiments. The results revealed that the hydrophilicity of the HEMA- g -PMMA IOL samples were significantly and permanently improved. Less platelets attachment was observed on the modified IOL, especially in the HEMA 2 - g -PMMA IOL group (with 1.65 × 10 −1 mol/L HEMA concentration), which also suppressed the proliferation of cells.
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高影响因子
ACS Omega
·
2026
·
IF 5.2
Kaidi Zhan,Yaxiling Wang,Jing Lin,Fengxu Chen,Xia Fan,Peiwen Hu,Jiangwen Wen,Fangduanyang Gong,Nan Zhang
Sensitive and selective detection of agricultural chemicals in complex matrices necessitates streamlined workflows without performance loss. By combining the inherent high sensitivity of photoelectrochemical (PEC) analysis with the high selectivity of enzymatic recognition, an immobilization-free PEC assay for the sulfonylurea herbicide chlorsulfuron (Chs) was developed, with purification steps omitted and analytical procedure simplified. Specifically, BiOI nanoflakes (NFs)/NiO heterojunction was fabricated as a sensing platform, generating strong and stable cathodic photocurrent in the presence of dissolved O2. The catalytic reaction of acetolactate synthase (ALS) consumed O2, quenching the photocurrent response. Conversely, upon addition of the model target Chs, a representative sulfonylurea herbicide and effective inhibitor of ALS, the signal was recovered. By monitoring the photocurrent change before and after the enzymatic reaction, Chs was quantified over a linear range of 0.25–100 μg/L, with a limit of detection (LOD) of 0.16 μg/L. The assay avoids laborious probe-immobilization steps by directly adding the enzyme into the electrolyte, significantly simplifying the detection protocol. Furthermore, by omitting traditional purification procedures, the overall workflow was further optimized. The method was successfully applied to detect Chs residues in wheat powder and to evaluate its accumulation in wheat seedlings, with accuracy and reliability validated by high-performance liquid chromatography (HPLC). This work achieves highly sensitive and immobilization-free PEC analysis of Chs and offers a feasible approach for sulfonylurea herbicide detection in selected agricultural samples, with streamlined pretreatment process and enhanced analytical efficiency.
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高影响因子
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