SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2021
·
IF 19.503
Hui Liu,Jianing Cheng,Wenjun He,Ying Li,Jing Mao,Xuerong Zheng,Cong Chen,Chunxiang Cui,Qiuyan Hao
Rationally design and exploration of bifunctional electrocatalysts with excellent performance towards water splitting is significant for hydrogen energy economy. Herein, a novel Au/Ni 3 S 2 heterostructure catalyst that was composed of self-supported Ni 3 S 2 nanosheets decorated with Au nanoparticles on Ni foam was fabricated. The bifunctional catalyst exhibited excellent catalytic activities towards oxygen evolution reaction (230 mV @ 10 mA cm −2 ) and hydrogen evolution reaction (97 mV @ 10 mA cm −2 ) in 1 M KOH. The electrolytic tank using the bifunctional catalyst only required 1.52 V to deliver 10 mA cm −2 and sustained for 60 h, outperforming most of advanced bifunctional catalysts. The X-ray absorption fine structure (XAFS) and density functional theory (DFT) calculations validated that the strong electronic coupling at the interface could modulate the electronic structure of Ni 3 S 2 , thereby optimizing the free energies of the adsorbed intermediates. This work provides an atomic-scale insight into the structure-properties relation of a promising heterostructure catalyst for water splitting.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Lingyuan Chen,Jiali Tang,Xinyue Zhang,Shixing Wang,Zhaogang Ren
A novel benzothiazole modified chitosan ( BCS ) with excellent Au(III) adsorption performance and selectivity was prepared as adsorbents. The structure and morphology of the adsorbents were characterized by FTIR, SEM, XRD and XPS. The adsorption property of the adsorbents for Au(III) were investigated under different reaction time, initial concentration of Au(III), temperature, pH and coexisting ions. The maximum adsorption capacity of BCS for Au(III) was 1072.22 mg/g at 298 K and optimal pH = 4, which was better than that of other adsorbents reported in literature. The adsorption kinetics and isotherm models fit the pseudo-second-order and Langmuir equations. This shows that the adsorption process of Au(III) is a monolayer chemical adsorption. The adsorption process can proceed spontaneously and belong to the endothermic reaction according to the thermodynamic results. The excellent adsorption performance is mainly attributed to the ion exchange and chelation of the nitrogen, sulfur and oxygen groups on the adsorbent with gold ions. Significantly, BCS has excellent selectivity toward Au(III) and remarkable recycle performance. With the high adsorption capacity, excellent selectivity and outstanding reusability, the BCS adsorbent could be a promising candidate to adsorb Au(III) from wastewater.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yuan Wang,Sen Liang,Mingliang Mei,Qiaowen Zhao,Guangwei She,Wensheng Shi,Lixuan Mu
Detecting the temperature of intracellular mitochondria with high sensitivity and stability is crucial to understanding the cellular metabolism and revealing the processes of mitochondria-related physiology. In this paper, employing the long fluorescence lifetime of modified Au nanoclusters (mAuNCs) by 4-(carboxybutyl) triphenylphosphonium bromide, we developed a fluorescence lifetime thermometer with high sensitivity and stability for the temperature of the intracellular mitochondria. A high relative temperature sensitivity of 2.8% and excellent photostability were achieved from the present thermometer. After incubation with L929 cells, the mAuNCs could be endocytosed into the cells and targeted the mitochondria, and the temperature changes at the L929 cells’ mitochondria, which were stimulated by carbonyl cyanide 3-chlorophenylhydrazone and Ca2+, were successfully detected via the fluorescence lifetime images of the mAuNCs. Furthermore, utilizing the mAuNCs, we clarified the effect of Mg2+ on the temperature of the intracellular mitochondria. The strategy of employing a material with a long fluorescence lifetime and remarkable stability to fabricate the fluorescence lifetime thermometer for mitochondria can be used to design various thermometers for other organelles.
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Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Wenquan Huang,Yuehua Deng,Liping Ye,Qiuling Xie,Yanbin Jiang
Poor safety and effectiveness is an outstanding challenge in the preparation of drug delivery systems (DDS) for cancer treatment. The pursuit of the high curative effect will inevitably increase the risk of adverse side effects. Herein, a bio-safe DDS was constructed by combining the advantages of functional zein and Au doped mesoporous silica nanoparticles ( [email protected] 2 ) to achieve chemo-photothermal therapy. The cRGD functionalized zein (cRGD-Zein) was coated on the surface of [email protected] 2 which effectively avoided premature leakage of paclitaxel and realized sustained drug release. Meanwhile, the high hemolysis rate (107%) of [email protected] 2 had been significantly reduced to 4%. The anti-hemolysis mechanism of functionalized zein was explored to give a deeper understanding of the interaction between nanoparticles and RBCs. The results showed that the functional zein would change the protein conformation during the interaction with [email protected] 2 to protect the RBCs from the damage of [email protected] 2 . And the release rate of hemoglobin was limited by the size of RBCs membrane cracks with approximately 40 nm in width and 470 nm in length. The cell cytotoxicity and uptake assays showed that the prepared DDS exhibited low tumour cell viability (35%) and enhanced uptake performance (99.3%). This work suggested that the prepared nanoparticles could serve as a promising carrier to achieve safe and efficacious tumour therapy.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Danli Hu,Haiyan Cui,Xinyang Wang,Fang Luo,Bin Qiu,Weicheng Cai,Hui Huang,Jian Wang,Zhenyu Lin
An Au/GaN photoelectrode was prepared by sputtering 30 nm thick Au film on the surface of n-type gallium nitride (GaN). When the electrode contacts with multilayered molybdenum disulfide (MoS2), photogenerated electrons and photogenerated holes transfer to MoS2 because of the band gap matching of MoS2 and GaN. The presence of Au promotes charge transfer and results in a greater recombination of electrons and holes; by this means, a more significant suppression of photocurrent can be detected. This characteristic has been coupled with the high selectivity of an aptamer and applied to develop a novel photoelectrochemical aptasensor for cancer biomarkers (alpha-fetoprotein (AFP) as a model). The aptamer of AFP was modified on the surface of the Au/GaN photoelectrode by Au–S bonds, which can bind to the target protein with high selectivity. Then, the transfer process of the charge carriers of GaN to MoS2 can be blocked by the target protein so that the suppression of photocurrent is reduced. The difference of the photocurrent in the presence and absence of AFP (ΔI) showed a linear relationship with AFP concentration that ranged from 1.0–150 ng/mL (R2 = 0.9995), and the detection limit was 0.3 ng/mL. The standard addition recovery rates ranged from 85.2 to 91.7%. The method possessed good sensitivity and high selectivity for AFP detection. The developed biosensor can be modified to detect other cancer biomarkers by simply replacing the aptamer used.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Xuemin Xia,Yijin Weng,Lei Zhang,Ruyi Tang,Xia Zhang
Surface-Enhanced Raman Scattering (SERS) is a powerful analysis technology, attracting more and more attention due to its high sensitivity and selectivity. Herein, we report a simple seed-mediated method to synthesize Au@Ag nanoparticles (NPs) as a multifunctional biosensor for the label-free detection of hydrogen peroxide (H 2 O 2 ) and glucose by SERS. Au@Ag NPs, as an ultrasensitive SERS substrate, show the dual activities (peroxidase-like and GOx-like activities). Under the condition of pH 4.0 NaAc buffer solution, the glucose and H 2 O can be catalyzed by Au@Ag NPs to produce glucose acid and H 2 O 2 , and then H 2 O 2 can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue oxidation product oxidic TMB (oxTMB) which exhibits strong SERS signals at 1188, 1330, 1605 cm −1 . Thus, we have developed a new SERS strategy for analysis of glucose with a detection limit of 5 × 10 − 10 mol L −1 , suggesting that Au@Ag NPs have the potential for biosensor, immunoassay and medical treatment.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 21
·6823-6833
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IF 7.393
Yingjiao Zhai,Hui Yang,Sinan Zhang,Jinhua Li,Kaixi Shi,Fangjun Jin
The controllability of materials that has attracted widespread attention is a key factor for improving the performance of SERS substrates. Hence, in this study, a kind of controllable materials constructed with Au nano-disk array and monolayer MoS2 had been developed. The optical properties of the composite were studied and the Raman enhancement was obtained. Compared with the monolayer MoS2, the peak of the above composite was slightly red-shifted, and the intensity was increased by about 2.5 times. This was due to the thermal effect and the internal quantum efficiency improvement of MoS2 caused by the local surface plasmon resonance of Au. The simulation results of the finite-difference time-domain and the experimental results all showed an enhanced photoluminescence of the composite, which was attributed to the resonance wavelength of the Au nano-disk being adjusted to be consistent with the photoluminescence peak of the monolayer MoS2. Through the synergistic action of the electromagnetic enhancement and chemical enhancement, the highly sensitive and stable surface-enhanced Raman scattering (SERS) active substrate constructed with Au-MoS2 composite was sequentially utilized in the detection of antibacterial agent residues on the aquatic products. Thereafter, a calibration curve with ultralow limits of detection (LOD) at 10−15 M was established for the standard solutions of crystal violet (CV), and then the assay on shrimps was successfully realized. Therefore, this research provides an effective way for attractive SERS performance of MoS2, and confirms the potential possibility for application on food safety and other trace detection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Gang-gang Zhang,Xiao-cui Lai,Neng-shui Ding,Qi-rong Xiong,Shuai Hou,Hong-wei Duan,Wei-hua Lai
Flower-like gold nanoparticle (AuNF) is widely used as a probe. However, the performance of conventional AuNF is not satisfactory because of complex synthesis, insufficient uniformity, and ordinary optical performance. In this paper, a novel chrysanthemum-like Au@polydopamine (AuNC@PDA) prepared using one-pot method was compared with conventional AuNF@polydopamine (AuNF@PDA) prepared using three-step method. AuNC@PDA had more branch structures with more uniform petaloid shape and narrower particle size distribution compared with AuNF@PDA. The grayscale of AuNC@PDA both in aqueous solution and on nitrocellulose membrane was approximately threefold higher than that of AuNF@PDA. Because of PDA coating, the stability and coupling efficiency of AuNC@PDA were much higher than that of conventional nanoprobes − AuNF and AuNS (gold nanosphere). These results indicated that AuNC@PDA had superior morphological and optical performance. The AuNC@PDA was first utilized as a probe in immunochromatographic assay (ICA), and compared with conventional AuNF@PDA. The limits of detection (LOD) of ICA based on AuNC@PDA, AuNS, and AuNF@PDA were 1.59, 9.58, and 5.13 mIU/mL, respectively, when human chorionic gonadotropin was detected as the target. The LOD of ICA based on AuNC@PDA had 6.0-fold and 3.3-fold improvement compared with that based on AuNS and AuNF@PDA, respectively. Our results could be used as a basis for developing novel probes in practical ICA applications.
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CELL PROLIFERATION
·
2021
·Vol. 54
·期 4
·e13008
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IF 6.831
Yifang Yuan,Ronghui Zhou,Ting Li,Shuang Qu,Hua Bai,Jiawu Liang,Xiaoxiao Cai,Bin Guo
Objectives Au nanoclusters (AuNCs) have been used widely in fluorescence bio-imaging because of their good fluorescence, small particle size and non-cytotoxicity. AuNCs are also efficient in computed tomography (CT) imaging. Hence, a dual-modal imaging probe can be constructed without any complicated modification processes by exploiting the excellent performance of AuNCs. In the present study, AuNCs were enriched with mesoporous silica nanoparticles (MSNs) to obtain enhanced fluorescence/CT dual-modal imaging, which was capable of acquiring more imaging information for diseases compared with single-mode imaging. Materials and methods Biocompatible bovine serum albumin (BSA)-capped AuNCs were prepared and loaded into amine-functionalized MSNs to form MSN@AuNCs. BSA-AuNCs, MSNs, and MSN@AuNCs were characterized by ultraviolet-visible (UV-vis) spectra, transmission electron microscopy (TEM), fluorescence spectra, and zeta potential. CT imaging was recorded using micro-CT scanning. Fluorescence imaging was measured using confocal laser scanning microscopy and flow cytometry. Results The prepared AuNCs and MSNs possessed good properties as previously reported. The fluorescence intensity and CT value of the AuNCs were enhanced after being enriched with MSNs. The nanoparticles were both non-cytotoxic. Confocal laser scanning microscopy and flow cytometry indicated that MSN@AuNCs in CAL-27 cells showed improved fluorescence imaging compared with simple AuNCs at the same concentration. Conclusions The results revealed that the strategy of enriching AuNCs with MSNs can obtain highly sensitive fluorescence/CT dual-modal imaging, which indicated the potential of this nanoparticle in the diagnosis and treatment of disease.
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JOURNAL OF CLUSTER SCIENCE
·
2020
·Vol. 32
·期 5
·1371-1379
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IF 1.731
Zhang Yingzhen,Song Yingying,Li Yancai
A simple and environment-friendly autocatalytic reduction process was developed for synthesis of Au/Ni(OH) 2 nanocomposites. The nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). It is found the nanocomposites are composed of ultrathin Ni(OH) 2 nanosheets and gold nanoparticles (AuNPs), and the AuNPs disperse evenly on the Ni(OH) 2 nanosheets. The electrochemical and electrocatalytic properties of the Au/Ni(OH) 2 modified glassy carbon electrode (Au/Ni(OH) 2 /GCE) were investigated by electrochemical experiments. Furthermore, through optimizing Au loading of the nanocomposites, the 5%-Au/Ni(OH) 2 /GCE displays the best electrocatalytic activity towards glucose oxidization in alkaline medium. Under optimal conditions, the 5%-Au/Ni(OH) 2 /GCE displays a wide linear range of 0.01 to 3.15 mM (R 2 = 0.998) to glucose. Besides, the sensor has a low detection limit of 0.10 μM (S/N = 3) and a high sensitivity of 342.1 μA·mM −1 ·cm −2 . It also displays satisfying stability, good reproducibility and selectivity.
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DYES AND PIGMENTS
·
2021
·
IF 4.889
Guojuan Qu,Tao Jiang,Wenjing Li,Tao Liu,Xiang Ma
Au nanoclusters (AuNCs) as a novel type of luminescent nanomaterial have been extensively explored due to low toxicity, excellent photostability and ultrafine size. However, few progresses have been made in producing ultrabright, homogeneous, functional, water-soluble AuNCs. In this regard, we propose an outstanding cross-linker acrylamide modified [email protected] ( [email protected] ) based on a simple one-step pretreatment , achieving a high quantum yield and self-healing hydrogels by copolymerizing with acrylamide. The plentiful network structure of the hydrogel effectively restricts the vibration of the ligands on the AuNCs surface, resulting in a 4-fold enhancement of quantum yield (from 5.6% to 22.8%). Meanwhile, the prepared [email protected] hydrogel exhibits self-healing capability and excellent elasticity. It is worth noting that AuNCs are used as cross-linkers in the polymerization reaction for the first time, which supplies a platform to realize the functionalization of gold nanoclusters.
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