JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2017
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IF 1.9
Kwanghyok Jong,Benzhi Ju,Shufen Zhang
In this study, a novel type of pH-responsive polymer PyHES-NAC (2-hydroxy-3-(2-propynyloxy) propyl hydroxyethyl starch (PyHES)) – (N-acetyl-cysteine (NAC)) was synthesized. First, PyHES was prepared via hydrophobic modification of hydroxyl groups in hydroxyethyl starch (HES) with propynylglycidyl ether (PGE), and then pH-responsive carboxylic acid group was connected to propynyl group via thiol-yne click reaction with NAC. Aqueous PyHES-NAC solutions exhibited a good transference between hydrophobic (or self-assembly) and hydrophilic static along with the change of pH value and protective properties of drugs under acidic conditions. 10.0% DOX was released under artificial gastric fluid after 2 h, whereas an immediate release (above 80%) was observed under artificial intestinal fluid. Drug loading capacity of PyHES-NAC was increased by the increase of degree of substitution (DS) of hydrophobic propynyl groups in PyHES, and 41 wt% DOX Loading capacity was the highest value in our study area.
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Nanomaterials
·
2022
·Vol. 12
·期 15
·2587
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IF 5.719
Gaihuan Ren,Zhanzhao Li,Dongxu Lu,Bo Li,Lulu Ren,Wenwen Di,Hongqin Yu,Jianxin He,Dejun Sun
Herein, we describe pH and magnetism dual-responsive liquid paraffin-in-water Pickering emulsion stabilized by dynamic covalent Fe3O4(DC-Fe3O4) nanoparticles. On one hand, the Pickerinfigureg emulsions are sensitive to pH variations, and efficient demulsification can be achieved by regulating the pH between 10 and 2 within 30 min. The dynamic imine bond in DC-Fe3O4can be reversibly formed and decomposed, resulting in a pH-controlled amphiphilicity. The Pickering emulsion can be reversibly switched between stable and unstable states by pH at least three times. On the other hand, the magnetic Fe3O4core of DC-Fe3O4allowed rapid separation of the oil droplets from Pickering emulsions under an external magnetic field within 40 s, which was a good extraction system for purifying the aqueous solution contaminated by rhodamine B. The dual responsiveness enables Pickering emulsions to have better control of their stability and to be applied more broadly.
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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
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2013
·Vol. 52
·期 5
·671-679
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IF 3.543
Chunsheng Xiao,Yilong Cheng,Yu Zhang,Jianxun Ding,Chaoliang He,Xiuli Zhuang,Xuesi Chen
The systemic investigation of the structural impacts of side chains on the pH- and thermo-responsiveness of tertiary amine functionalized poly( l -glutamate)s (TA-PGs) was carried out. The TA-PGs polymers were effectively synthesized by Cu(I)-catalyzed azide-alkyne cycloaddition click reaction of azido tertiary amines with poly(γ-propargyl- l -glutamate) (PPLG). Turbimetric measurements were performed to characterize the pH- and temperature-induced phase transition of TA-PGs in aqueous solution, which suggested a structural dependence of the properties on the N -substituted groups and the “linkers” between 1,2,3-triazole ring and the tertiary amine groups in the side chains. In detail, the pH responsive properties of TA-PGs were basically determined by the hydrophobicity of the N -substituted groups in the side chains and the pH transition point (pH t ) decreased as the increasing hydrophobicity of the N -substituted groups, while the temperature-responsiveness of TA-PGs were affected by either the N -substituted groups or the “linkers.” TA-PGs with a moderate N -substituted amine group (e.g., DEA, PR, and PD) or a branched “linker” (e.g., iso -propylene and 2-methylpropylene group) were more likely to express the LCST-type phase transition tuned by pH variation. These structure–property relationships revealed in this study would help to develop the applications of TA-PGs in smart drug delivery systems. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014 , 52 , 671–679
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Analytical Methods
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2025
·
IF 2.6
Yue Wang,Hui Ju,Peng Yan
Traditional chemical sensor arrays require multiple probes as sensing elements, making their construction complex and costly. To address this limitation, we designed a simple and sensitive two-dimensional fluorescence sensor array for protein identification. The sensor array employs six pH buffer solutions and two osmolytes as sensing elements, with CdSe/ZnS quantum dots (QDs) serving as fluorescent probes. Proteins in different pH environments exhibit variations in surface charge and three-dimensional conformation, which are further amplified by the addition of osmolytes. These enhanced differences result in distinct fluorescence response patterns through differential protein–QD interactions. Using linear discriminant analysis (LDA), 13 proteins were accurately classified. The sensor array was further applied to discriminate protein mixtures and proteins at different concentrations. These results indicate that the proposed sensor array provides a simple, rapid, and cost-effective strategy with promising applications in clinical diagnostics and proteomics.
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高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Keyi Long,Tiao Han,Wenxi Hu,Fuping Nie,Mei Yang,Jun Li,Changjun Hou,Danqun Huo
Loop-mediated isothermal amplification (LAMP) has been used as one of the tools for nucleic acid detection. The pyrophosphoric acid (PPi) produced by LAMP leads to a change in the pH value of the solution, and acid-base indicators can be used to distinguish positive from negative. However, the microliter reaction system and low concentration target cause small changes in pH, sensitive pH indicators are urgently needed. Herein, we developed a one-step solvothermal approach to synthesize biomass-derived carbon dots named FA-bioCDs. Compared with hydrothermal synthesis, the solvothermal approach red-shifted the emission maximum to 535 nm, and made FA-CDs pH-sensitive in the range of 8.16–9.12, so it can be used for LAMP endpoint detection. The relatively alkaline LAMP buffer reduces the fluorescence of FA-bioCDs. Rapid LAMP accumulates hydrogen ions, lowering the pH and “turning on” FA-bioCDs fluorescence. Thus, LAMP acts as a pH-mediated fluorescence switch for FA-bioCDs. The strategy was used for human papillomavirus DNA detection, and the detection limit was as low as 2.450 copies·μL −1 in about 30 min. LAMP ensures rapid and sensitive detection, and the relatively long emission wavelength of FA-bioCDs may reduce interference in practical detection scenarios. The development of LAMP-FA-bioCDs not only offers a rapid and environmentally friendly tool for nucleic acid detection in clinical settings but also bridges the gap between agriculture and medicine.
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MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Xiaoju Zhou,Chenyuan Wang,Haojie Ke,Rui Zhang,Xiaoya Yu,Wei Li,Shi Chen,Lianrong Wang
Melanoma is one of the most common and aggressive skin malignancies with unsatisfactory treatment effects due to the serious side effects, multidrug resistance, and poor prognosis. Chemotherapeutic transdermal delivery has emerged as a highly effective and novel strategy for melanoma therapy. In this study, we chose hydrophobic 10-hydroxycamptothecin (HCPT) as a model drug and developed novel microneedle (MN) patches integrated with cRGD-modified pH-sensitive HCPT liposomes (cRGD-LP-HCPT MN patches). The approach not only enhances the drug’s solubility and stability but also allows for high targeting specificity and maximizing intracellular drug accumulation in melanoma cells. The cRGD-modified pH-sensitive HCPT liposomes (cRGD-LP-HCPT) were prepared by using a thin-film hydration-ultrasonic method. cRGD-LP-HCPT exhibited high drug encapsulation efficiency (94.78%), small size (∼102.91 nm), good stability, and a profile of a rapid drug release triggered by acidic conditions. Meanwhile, it displayed higher cytotoxic effects, with an IC50 value of 1 μg/mL, compared to HCPT or the liposomes without modification. Consequently, to facilitate the transdermal delivery of HCPT into deep tumor tissues, cRGD-LP-HCPT were assembled into water-soluble needle tips of the microneedles. The cRGD-LP-HCPT MN patches exhibited excellent mechanical properties. Successful needle penetration into the pig skin with a distribution at a depth of about 200 μm was observed. Furthermore, in contrast to the systemic distribution profile of intravenous cRGD-LP-IR780, cRGD-LP-IR780 MN patches led to highly localized and potent tumor-specific fluorescence with negligible exposure to normal tissues. Ultimately, this novel HCPT formulation exhibited significant antitumor efficiency and minimal side effects. These results demonstrated that the cRGD-LP-HCPT MN patch could be a promising drug delivery system for melanoma treatment in the clinic.
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高影响因子
Current Drug Delivery
·
2025
·Vol. 22
·期 10
·1469-1480
·
IF 3
Haifeng Zhang,Qiuhui Wang,Shangdong Wang,Ruiyao Zhou,Jianwu Cai,Xiao Hu
BackgroundPancreatic cancer is a highly malignant tumor with a poor prognosis, and current treatment methods have limited effectiveness. Therefore, developing new and more effective therapeutic strategies is crucial. This study aims to establish pH-responsive silk fibroin (SF) nanoparticles encapsulating β-hydroxyisovalerylshikonin (SF@β-HIVS) to enhance the therapeutic effects against pancreatic cancer.MethodsSF@β-HIVS nanoparticles were prepared using a self-assembly technique and characterized under different pH conditions using scanning electron microscopy (SEM) and dynamic light scattering (DLS). The effects of SF@β-HIVS on the viability, apoptosis, and migration of PANC-1 cells were assessed through in vitro experiments. Additionally, in vivo experiments using a PANC-1 xenograft mouse model evaluated the antitumor activity and biosafety of SF@β-HIVS.ResultsSF@β-HIVS nanoparticles exhibited a uniformly distributed spherical structure under pH 7.4 conditions and rapidly disintegrated in acidic environments, releasing the drug. In vitro experiments demonstrated that SF@β-HIVS significantly inhibited PANC-1 cell proliferation, induced apoptosis, and suppressed cell migration. In vivo, experiments confirmed the significant antitumor activity and good biosafety of SF@β-HIVS.ConclusionThis study successfully developed pH-responsive SF@β-HIVS nanoparticles and validated their potential in treating pancreatic cancer. These findings provided a foundation for the clinical application of SF@β-HIVS in pancreatic cancer treatment.
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EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Dan Ji,Yaping Wang,Mengqing Luo,Zihao Zhang,Xiaohua Chang,Yutian Zhu
Dual-responsive shape-transformable block copolymer (BCP) particles have arisen great attentions because of their tunable physical and chemical characteristics triggered by external stimuli. Herein, we developed a simple yet effective strategy to realize pH and light dual-triggered shape-switchable BCP particles through the co-assembly of BCPs with photo-responsive additive 4-hydroxyazobenzene (Azo-OH) within the evaporation emulsion droplets. The formation of hydrogen bonding interactions between the Azo-OH and 4-vinylpyridine (4VP) unit increases the volume fraction of P4VP domain, in turn leading to a morphological transition of the particles from onions to pupas by tuning the Azo-OH content. Subsequent light-induced trans – cis transition of the Azo unit induces the enhancement of the hydrophilicity of the P4VP domains, giving rise to internal shape transitions of the BCP particles from pupas to onions with P4VP at the outmost-layer. Furtherly, since the hydrogen bonds between Azo-OH and 4VP group are sensitive to pH, the interfacial properties of the emulsion droplets and the assembled structures are tuned through adjusting the environment pH of the aqueous solution, causing the formation of various particle shape, including pupas, raspberries, and onions. This work offers a promising method to engineer the microstructures of polymeric assemblies, which may attract more attention on the applications of the stimuli-responsive particles.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Yuanhao Sheng,Yufu Li,Huizhong Hu,Jinhong Zhong,Xin Zhong,Feilin Wang,Haihui Pan,Yongguang Bi,Fansheng Kong
Apigenin(AP) exhibits significant biological activities and pharmacological effects, including antioxidant, hypoglycemic, lipid-lowering, and anti-inflammatory properties. However, its application is limited by its low solubility, poor bioavailability, and instability. Gelatin is commonly used as a drug delivery carrier. In this study, apigenin-gelatin nanoparticles(AP-GNPs) were prepared using a pH-driven method to better explore the practical applications of AP. Characterization of AP-GNPs using FT-IR, XRD, SEM and TEM revealed that apigenin was bound to gelatin via hydrogen bonding and encapsulated within, forming a spherical amorphous structure.The particle size, PDI, zeta potential, encapsulation efficiency, and loading content of AP-GNPs were 365.3 ± 7.7 nm, 0.209 ± 0.022, −11.53 ± 0.82 mV, 83.7 ± 2.2 %, and 9.3 ± 0.3 %, respectively. Stability tests and gastrointestinal simulation digestion experiments demonstrated that AP-GNPs exhibited superior stability, solubility, and bioaccessibility compared to AP. Additionally, AP-GNPs provided protection against AAPH-induced erythrocyte haemolysis and showed inhibitory effects on the activities of α-glucosidase and α-amylase. In summary, the significant enhancement of the solubility, stability, and bioaccessibility of AP by AP-GNPs offers novel insights for the extensive application of apigenin in the pharmaceutical and food industries.
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Frontiers in Pharmacology
·
2025
·
IF 4.8
Dapeng Li,Yuanfan Li,Jie Cang,Xianwen Yan,Feipeng Wu,Xuan Sun,Wenchao Zhang
Introduction: Osteosarcoma (OS) is the most common primary malignant bone tumor in pediatric populations. Its treatment is complicated by chemotherapy-induced toxicity and limited induction of immunogenic cell death (ICD).Methods: To address these challenges, we developed a pH-responsive, multi-component nanoparticle system designed to co-deliver doxorubicin (DOX), monophosphoryl lipid A (MPLA), and a PD-1/PD-L1-targeting peptide, integrated with the immune-modulating polymer PEG-PC7A. The system was optimized using both one-factor-at-a-time (OFAT) and Box-Behnken design (BBD).Results: The optimized nanoparticles had a hydrodynamic size of 110 nm, high encapsulation efficiency (97.15%), and pH-sensitive drug release (91% at pH 6.5). In vitro studies showed enhanced ICD markers, including calreticulin exposure and ATP/HMGB1 release, aswell as synergistic dendritic cell maturation via dual STING/TLR4 pathway activation. In an orthotopic LM8 osteosarcoma model, the nanoparticles significantly suppressed tumor growth, promoted cytotoxic T lymphocyte infiltration, reduced regulatory T cells, and established long-term immune memory.Discussion: The combination of ICD induction, innate immune activation, and checkpoint blockade reprogrammed the tumor microenvironment, amplifying anti-tumor immune responses. These results demonstrate the potential of this multifunctional nanoparticle platform as an effective immunochemotherapeutic strategy for osteosarcoma, offering enhanced therapeutic efficacy and reduced systemic toxicity.
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Journal of Environmental Sciences
·
2019
·
IF 3.556
Hongyan Sun,Ruyuan Jiao,Hui Xu,Guangyu An,Dongsheng Wang
In order to evaluate the influence of particle size and particle concentration on the coagulation process, two kinds of particle suspensions, nanoparticles and microparticles , were employed to investigate the effect of particle size on coagulation mechanisms with varying coagulation parameters. Results showed that it is easier for nanoparticles to cause self-aggregation because of Brownian motion, while interception and sedimentation are the mainly physical processes affecting particle transport for microparticles, so they are more stable and disperse more easily. The particle size distribution and particle concentration had distinct influence on the coagulation mechanisms. Under neutral conditions, as the amount of coagulant increased, the coagulation mechanism for nanoparticles changed from charge neutralization to sweep flocculation and the nanoparticles became destabilized, re-stabilized and again destabilized. For microparticles, although the coagulation mechanism was the same as that of nanoparticles, the increased rate of aluminum hydroxide precipitation exceeded the adsorption of incipiently formed soluble alum species, resulting in the disappearance of the re-stabilization zone. Under acidic conditions, Brownian motion dominates for nanoparticles at low particle concentrations, while sweep flocculation is predominant at high particle concentrations. As for microparticles, charge neutralization and sweep flocculation are the mechanisms for low and high particle concentrations respectively. Under alkaline condition, although the mechanisms for both nano- and microparticles are the same, the morphology of flocs and the kinetics of floc formation are different. At low particle concentrations, nanoparticles have larger growth rate and final size of flocs, while at high particle concentrations, nanoparticles have higher fractal dimension and recovery factors.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Shasha Yan,Lanfang Chai,Wei Li,Ling-Ping Xiao,Xiaohong Chen,Run-Cang Sun
Although pH-sensitive lignin-based materials have extensively attracted attentions because of the smart response to pH, it is still a challenge to realize the regulation of the pH-responsive properties. Focusing on this problem, we proposed a new method by tunning the content of hydroxyl groups to adjust the performances of lignin-based hydrogel (L-HG). The modified lignins with the hydroxyl content ranged from 0.31 to 5.91 mmol/g were obtained after acetylation or demethylation . As the results, when increasing the hydroxyl content, the gels became more compact and smooth, and the tensile strength reached 4.10 MPa. The strain was up to 83.2%, and the bending behavior occurred more easily at various pH (3, 5, 9, 11 and 13). While decreasing the hydroxyl content resulted in the impairments of almost all of the above performances. Based on the test of the moisture content, the pH-sensitive bending behavior might be mainly caused by the opposing forces of hydrophilic and hydrophobic interaction. Finally, the application test showed that the trimmed hydrogel could hook up 10 times weight greater than itself under both acid and alkaline conditions. We believe that this work would provide the theoretical guidance for the regulation of pH sensitivity of L-HG.
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