BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Da-Wei Zhou,Meng Yin,Yun Shen,Xiao-Xue Wang,Chen-Yu Wang,Ke-Zheng Chen,Qi Fang,Sheng-Lin Qiao
Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by the loss of dopaminergic neurons and the consequent decline in motor and cognitive functions. The primary therapeutic agent levodopa necessitates precise dosing due to its narrow therapeutic window and complex pharmacokinetics. This study presents the development of a novel CuCoFe-LDHzyme-based sweat sensor for real-time monitoring of levodopa concentration in PD patients. Employing differential pulse voltammetry (DPV) technique, the sensor demonstrates high sensitivity and selectivity, achieving a detection limit of 28.1 nM. The sensor's design allows for non-invasive, continuous monitoring, significantly enhancing patient convenience compared to traditional blood sampling methods. Through pH correction, precise quantification of levodopa in sweat is accomplished, and a strong correlation (Pearson coefficient = 0.833) with blood levodopa levels is established. The pharmacokinetic profile of levodopa is reconstructed in real-time, offering a promising tool for optimizing PD treatment regimens. This study highlights the potential of CuCoFe-LDHzyme sensors to advance personalized treatment strategies, aiming to improve the quality of life for PD patients by providing clinicians with real-time data for medication adjustments.
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高影响因子
FOOD CHEMISTRY
·
2024
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IF 8.5
Xia Niu,Wen-Yuan Pei,Jian-Fang Ma
Herein, a novel FeCoNi(b)-800 ternary metal nanoalloy was uniformly mixed with reduced graphene oxide (RGO) to synthesize the FeCoNi(b)-800@RGO(2:1) composite. The addition of RGO not only stopped the accumulation of FeCoNi(b)-800 alloy, but also heightened the electrocatalytic activity of composite. Particularly, the FeCoNi(b)-800@RGO(2:1) composite displayed the significantly strong electrocatalytic capacity for the reduction of roxarsone (ROX). Furthermore, the FeCoNi(b)-800@RGO(2:1) composite possessed enough porosity and metal catalytic sites, facilitating the transport and electrochemical reduction of the ROX. Thus, the FeCoNi(b)-800@RGO(2:1) composite modified glassy carbon electrode (FeCoNi(b)-800@RGO(2:1)/GCE) showed the superb electrochemical detection effect for ROX with relatively wide working range (0.1–1500 μM) and low detection limit (0.013 μM). Importantly, the FeCoNi(b)-800@RGO(2:1)/GCE sensor could accurately determine the contents of ROX in actual pork, chicken, duck and egg samples, indicating that it had good suitability in food safety monitoring.
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高影响因子
ANALYST
·
2024
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IF 3.6
Yi Zhang,Xue Kong,Haiyang Guo,Jing Wang,Zheng-Zhi Yin
An electrochemical microsensor for mesothelin (MSLN) based on an acupuncture needle (AN) was constructed in this work. To prepare the microsensor, MSLN was self-assembled on 4-mercaptophenylboric acid (4-MPBA) by an interaction force between the external cis-diol and phenylboronic acid. This was followed by the gradual electropolymerization of thionine (TH) and eriochrome black T (EBT) around the anchored protein. The thickness of the surface imprinted layers influenced the sensing performance and needed to be smaller than the height of the anchored protein. The polymerized EBT was not electrically active, but the polymerized TH provided a significant electrochemical signal. Therefore, electron transfer smoothly proceeded through the eluted nanocavities. The imprinted nanocavities were highly selective toward MSLN, and the rebinding of insulating proteins reduced the electrochemical signal of the embedded pTH. The functionalized interface was characterized by SEM and electrochemical methods, and the preparation conditions were studied. After optimization, the sensor showed a linear response in the range of 0.1 to 1000 ng/mL with a detection limit of 10 pg/mL, indicating good performance compared with other reported methods. This microsensor also showed high specificity and stability, which can be attributed to the fine complementation of the imprinted organic nanocavities. The sensitivity of this sensor was related to the nanocavities used for electron transport around the AuNPs. In the future, microsensors that can directly provide electrochemical signals are expected to play important roles especially on AN matrices.
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Materials Today Communications
·
2025
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IF 4.5
Yi Zhang,Hao Wang,Man Li,Xinhao Sun,Yan Zhang,Zhifeng Wang,Chunling Qin
This work successfully synthesizes a new type of multi-layered flexible hybrid electrode (Cu/Ni(OH) 2 /np-Ni) composing of bimetallic components, hierarchical porous structure and outstanding glucose electrocatalytic performance. The synthesis strategy includes a facile two-step approach, i.e. the Ni(OH) 2 nanolayer in-situ grows on a nanoporous Ni (np-Ni) network (Ni(OH) 2 /np-Ni) via deionized water immersion and then the metallic Cu dendrites are uniformly deposited on Ni(OH) 2 /np-Ni. Moreover, due to the hierarchical porous structure as well as synergetic effect of Ni(OH) 2 and Cu dual electroactive components, the Cu/Ni(OH) 2 /np-Ni hybrid electrode electrodeposited with 1 min delivers wider linear range of 18 mM and higher sensitivity of 1.629 mA mM −1 cm −2 . The electrode possesses excellent interference immunity, reproducibility as well as long-time stability. In addition, the mechanism of electrochemical sensors for detecting glucose under alkaline conditions is briefly introduced. Accordingly, the flexible Cu/Ni(OH) 2 /np-Ni electrode material shows promise for further development as a contender for wearable glucose sensors.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
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IF 4.8
Jianan Yang,Tonglian Jiang,Xun Lu,Xiang li,Xuling Zhou,Xinxin Guo,Chengxin Ma,Xiaobei Xie,Dongxiao Li,Siqi Yu,Jiayi An,Binghai Zhao,Hongzhi Li
Hyperuricemia is a known risk factor for chronic kidney disease (CKD) and subsequent renal fibrosis. N6-methyladenosine (m6A) is the most prevalent chemical modification in eukaryotic mRNAs and has been implicated in various diseases. However, its role in hyperuricemic nephropathy (HN) remains unclear. This study investigated the involvement of the methylase METTL14 in HN pathogenesis. Our in vitro and in vivo function experiments demonstrated that METTL14 plays a crucial role in HN. In mouse models of uric acid (UA)-induced renal injury, we detected impaired kidney function, increased renal interstitial fibrosis, and significantly decreased m6A methylation levels in renal tissues. Treatment with benzbromarone, a UA-lowering drug, alleviated renal injury, restored m6A methylation levels, and upregulated METTL14 expression. Cellular experiments showed that METTL14 overexpression attenuated high UA-induced fibrosis in renal tubular epithelial cells. This overexpression significantly decreases the expression of GLUT9, a key protein involved in UA transport, leading to reduced UA reabsorption. Additionally, MeRIP-qPCR and dual-luciferase reporter gene experiments further demonstrated that METTL14 overexpression enhanced Glut9 mRNA m6A methylation modification, accelerating its degradation and decreasing expression levels. Thus, METTL14-mediated RNA m6A modification plays a role in the renal tubular epithelial cell damage induced by high UA, by regulating Glut9 mRNA post-transcriptionally. These findings provide valuable insights for the diagnosis and development of therapeutic drugs for HN.
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ACS Applied Materials & Interfaces
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2024
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IF 8.3
He Zhao,Ling Zhang,Tianbo Deng,Chunzhong Li
Wearable sweat sensors have received considerable attention due to their great potential for noninvasive continuous monitoring of an individual’s health status applications. However, the low secretion rate and fast evaporation of sweat pose challenges in collecting sweat from sedentary individuals for noninvasive analysis of body physiology. Here, we demonstrate wearable textiles for continuous monitoring of sweat at rest using the combination of a heating element and a microfluidic channel to increase localized skin sweat secretion rates and combat sweat evaporation, enabling accurate and stable monitoring of trace amounts of sweat. The Janus sensing yarns with a glucose sensing sensitivity of 36.57 mA cm–2 mM–1 are embroidered into the superhydrophobic heated textile to collect sweat directionally, resulting in improved sweat collection efficiency of up to 96 and 75% retention. The device also maintains a highly durable sensing performance, even in dynamic deformation, recycling, and washing. The microfluidic sensing textile can be further designed into a wireless sensing system that enables sedentary-compatible sweat analysis for the continuous, real-time monitoring of body glucose levels at rest.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiangxian Li,Hengfeng Zhu,Xuanru Wei,Quanling Yang,Chuanxi Xiong,Zhuqun Shi
Activated carbon is extensively utilized in blood purification applications. However, its performance has been significantly limited by their poor blood compatibility. In this work, 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibers (TOCN) and activated carbon (AC) were used to form composite beads by the drop curing method to improve hemocompatibility. The TOCN/AC composite beads had porous surface and exhibited extraordinary adsorption properties. The beads had a high adsorption capacity for creatinine with the optimal adsorption capacity of 83.33 mg g −1 . And the equilibrium adsorption of bilirubin, uric acid and Cu 2+ by TOCN/AC beads was as high as 159.80 mg g −1 , 114.61 mg g −1 and 154.0 mg g −1 , respectively, with a mass ratio of TOCN to AC of 1:4. It is also observed that the adsorption behavior of TOCN/AC beads on creatinine was consistent with the second-order kinetics and Langmuir isothermal model. The hemolysis rate of TOCN/AC was 1.21 %, indicating that TOCN/AC beads had good blood compatibility. The clearance of creatinine toxin in blood by TOCN/AC beads was as high as 87 % within 90 min. Overall, our produced composite beads had great potential for application in the field of blood purification.
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高影响因子
BIOMACROMOLECULES
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2025
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IF 5.4
Xuanru Wei,Hengfeng Zhu,Dichan Hong,Xiangxian Li,Zhuqun Shi,Quanling Yang
Conventional hemoperfusion adsorbents suffer from inefficiency and poor biocompatibility. Cellulose, a natural polysaccharide with biocompatible, biodegradable, and nontoxic properties, was combined with graphene oxide (GO) to fabricate composite beads (TGO) for blood purification. GO synthesized via a modified Hummers method was complexed with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanofibrils (TOCNs). Increasing GO content (2–20 wt %) enhanced TGO’s specific surface area (256.4–289.0 m2 g–1) while retaining an ∼10 nm pore size. TGO demonstrated exceptional adsorption capacities: bilirubin (418.4 mg g–1), creatinine (23.5 mg g–1), uric acid (146.6 mg g–1), and Cu2+ (171.9 mg g–1). The beads exhibited excellent hemocompatibility (hemolysis rate <5%) and prolonged recalcification time (585 ± 5.2 s). Notably, TGO restored blood bilirubin levels to normal within 30 min, highlighting its potential for blood purification.
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MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Jie An,Chaoyan Zhang,Fei Yan,Peng Ma
Rapid and precise detection of tumor biomarkers in serum is of great significance for early diagnosis, treatment assessment, and recurrence monitoring of cancers. The fabrication of user-friendly electrochemiluminescence (ECL) aptasensors with effective signal amplification is highly desirable. In this study, an ECL aptasensor was developed with luminol-based ternary ECL platform using dissolved oxygen as endogenous co-reactant and nanochannel-confined platinum nanoparticles (PtNPs) as co-reactant promoter, which can realize highly sensitive detection of the tumor biomarker, carcinoembryonic antigen (CEA). On the cost-effective indium tin oxide (ITO) electrode, amino-modified vertically-ordered mesoporous silica film (NH 2 -VMSF) was easily grown to form high-density nanochannel array with ultrasmall nanochannels. PtNPs were then in-situ synthesized within the nanochannels via electrodeposition. Ternary ECL system was successfully fabricated as confined PtNPs can catalyze the electroreduction of dissolved oxygen at negative potentials as well as electrochemical oxidation of luminol, enhancing the ECL signal of luminol. The recognitive aptamer (Apt) was covalently immobilized on the outer surface of NH 2 -VMSF, resulting in the fabrication of an aptasensor. In presence of CEA, a decreased ECL signal was obtained as the formed complex on the recognition interface hindered the diffusion of ECL emitter to the supporting electrode. Based on this signal turn-off mode, sensitive detection of CEA was achieved ranged from 10 fg mL −1 to 100 ng mL −1 , with a low detection limit (DL, 0.4 fg mL −1 ). The aptasensor displayed high selectivity and good reproducibility. The nanochannel-based aptasensor and ternary ECL system demonstrates great potential in convenient and sensitive detection of cancer biomarker.
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Materials Today Communications
·
2024
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IF 3.7
Yicheng Zhou,Li Dong,Jiazheng Zhang,Rongrong Qiao,Lin Liu,Chao Liu,Xiaobo Zhang,Zhiwei Tong
In this study, a nanocomposite of Polyaniline/titanium carbide (PANI/Ti 3 C 2 ) was synthesized through self-assembly and in situ polymerization for use as a sensor material to simultaneously detect guanine (G), adenine (A), and uric acid (UA). X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were utilized to confirm the successful synthesis of PANI/Ti 3 C 2 . Electrochemical analysis methods including differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) were used to detect the electrochemical performance of PANI/Ti 3 C 2 modified glassy carbon electrode (PANI/Ti 3 C 2 /GCE). The pH dependence, electrochemically active surface area (ECSA), electron transport , stability, reproducibility, sensitivity, and selectivity of the modified GCE were examined. Compared to previous reports, the nanocomposite exhibited excellent performance. Uric acid, guanine, and adenine detection limits were 0.33, 0.31, and 0.28 μM, respectively, over concentration ranges of 0.62 - 20.10, 0.31 - 17.00, and 0.31 - 17.00 μM. The nanocomposite demonstrated high recovery and low error in serum sample detection. In summary, PANI/Ti 3 C 2 is a sensor material with excellent electrochemical properties .
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Nanoscale
·
2025
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IF 5.1
Xinda Xu,Chao Zhang,Woochul Yang,Yujia Li,Bing Li,Yuvaraj Haldorai,Jiang Zhenyu,Wanfeng Xie
Cobalt tetraoxide (Co3O4) holds great potential for enzymeless glucose detection due to its strong redox capabilities. However, its poor electrical conductivity limits the charge transfer and hence weakens its catalytic activity when utilized as an electrode in electrochemical sensors, such as an enzymeless glucose sensor. In this study, we developed an indium oxide (In2O3)-wrapped Co3O4 nanofibers (Co3O4@In2O3 NFs)-based electrochemical sensor for the enzymeless detection of glucose, with an ultra-low limit of detection (LoD) of 8.85 nM (S/N = 3), a wide detection range from 10 nM to 118 μM, and a high sensitivity of 1197.5 μA mM−1 cm−2. In addition, this sensor exhibits good selectivity, reproducibility, and long-term stability for over 30 days. The sensing performance of bare Co3O4 NFs towards glucose has been significantly enhanced by incorporating them within highly conductive In2O3, which is mainly attributed to the synergistic combination of the electrocatalytic activity of Co3O4 NFs, as the delicately designed core, and the excellent conductivity of In2O3, as the shell. Thus, this novel modified Co3O4@In2O3 NFs electrochemical composite has huge potential to be further developed for enzymeless glucose analysis in practical clinical settings.
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MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Yu-Ying Xin,Li-Hong Liu,Yan Liu,Zhao-Peng Deng,Xiao-Li Cheng,Xian-Fa Zhang,Ying-Ming Xu,Li-Hua Huo,Shan Gao
The development of electrochemical dopamine (DA) sensor with anti-interfering ascorbic acid and uric acid is challenging due to the presence of their overlapping electrical signals. Herein, MoS 2 nanoflower assembled from ultrathin nanosheets were uniformly loaded on rGO substrate through one-step hydrothermal reaction. Then, the composite (MoS 2 /rGO) was modified on the surface of glassy carbon electrode (GCE) by a drop coating method, successfully assembling non-enzymatic electrochemical sensor for ultra-selective DA detection. The test results show that the MoS 2 /rGO/GCE probe exhibits good sensitivity (15.73 μA μM −1 cm −2 ), small limit of detection (13 nM) and wide range of detection (0.1 ∼ 110.0 μM) for DA in 0.1 M PBS medium (pH = 7.4), as well as good interference resistance, reproducibility and stability. This excellent sensing ability mainly stems from the synergistic effect of the prepared electrode material with abundant edge defects, small charge transfer resistance, large electrochemically active area, and more negative Zeta potential (–23.4 mV). Furthermore, the satisfactory and accurate detection of DA content in animal serums predicts its potential for practical applications in related biological aspects.
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危险品化学品经营许可证（带存储）