INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Xianzhen Yin,Yiting Li,Congling Yang,Jie Weng,Jianxin Wang,Jie Zhou,Bo Feng
Macrophage phenotype conversion is crucial for improving post-traumatic angiogenesis and tissue repair. Biomaterials with the ability of skewing macrophage phenotype have attracted widespread attention in the field of tissue engineering. The aim of this study was to transform macrophage phenotype by a three-step process; anodizing, drug loading and coating with polyelectrolyte multilayer (PEM) films. Interleukin (IL)-4, an anti-inflammatory cytokine, was loaded into titania nanotubes (TNTs) on the titanium surface. Subsequently, sodium alginate (ALG) and chitosan (CS) were alternately assembled onto IL-4-loaded TNTs and cross-linked with genipin/calcium chloride, finally forming cross-linked PEM films. The IL-4 release profile and cellular immune response of the modified surface was investigated. In the simulated biological solution, only 20% of IL-4 were detected in the first 3 days, with a sustained release of approximately 5 ng over 10 days. The results of gene expression and protein secretion in macrophages indicated that IL-4-loaded PEM films significantly attenuated the inflammatory activity of macrophages at the later stage through down-regulating the mRNA and protein levels of inflammatory markers. In summary, IL-4 was controlled released from the cross-linked PEM films deposited on the nanotubes, leading to the temporal conversion of macrophage phenotype.
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International Journal of Polymer Science
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2019
·
IF 1.892
Wei Xiong,Yue-kun Shen,Peng Dong,Ying Xiao,Xiong-qing Huang,Wen-qi Huang,Lu Yang,Xia Feng
Sustained release of anesthesia has shown great promise in the treatment of chronic pain in patients. In this research, we used neutralized ropivacaine as an anesthesia and poly(ε-caprolactone) (PCL) with different architectures to systematically study how these architectures affect the release of ropivacaine. After optimizing the parameters of the preparation of microspheres, ropivacaine-loaded 1-PCL microspheres and 4-PCL microspheres were obtained. Fourier Transform infrared spectra (FT-IR) and X-ray diffraction spectra (XRD) confirmed that ropivacaine was encapsulated within the microsphere rather than inserted on the surface of the microsphere. Ropivacaine was found to be buried deeper in the 1-PCL microsphere than in the 4-PCL microsphere. In vitro release assay revealed that small crystalline grains interfered with ropivacaine release in 4-PCL microspheres during the initial release period, but then two kinds of microspheres showed a similar ropivacaine release rate. We basically proved that the architecture of PCL has a negligible effect on ropivacaine release. Cell proliferation test revealed that the release of products from the microspheres resulted in insignificant toxicity towards mammalian cells.
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WATER ENVIRONMENT RESEARCH
·
2019
·Vol. 91
·期 8
·770-779
·
IF 1.24
Jinhong Fan,Hehe Qin,Yunfei Zhang,Simin Jiang
In this study, Fe/Cu bimetal composite was prepared by high-energy ball milling (BM) method for the removal of refractory organics. The BM Fe/Cu bimetal was characterized by SEM-EDS, XRD, and XPS. Evenly distributed Fe and Cu was observed in the EDS mapping. In contrasting experiments, the removal rate of 4-chlorophenol (4-CP) by BM Fe/Cu materials was about 10-fold faster than that by chemical substitution deposition (CSD) of Fe/Cu material. Complete 4-CP removal and 66.7% of total organic carbon (TOC) mineralization in the BM Fe/Cu–O 2 system were achieved. Dissolved oxygen plays a crucial role for 4-CP degradation through the in situ generation of reactive oxygen species (ROS) such as H 2 O 2 , ·OH, and via oxygen activation reactions. The predominant reactive radicals were identified to be and ·OH through ESR technique and inhibition experiments. The coexistence of oxidation and reduction of 4-CP in the BM Fe/Cu-O 2 system was proposed. Practitioner points 4-CP removal rate by BM Fe/Cu is 10-fold faster than that by CSD Fe/Cu at the same conditions. Complete 4-CP removal and 66.7% of TOC reduction were achieved. All three ROS including ·OH, , and H 2 O 2 coexisted in the BM Fe/Cu-O 2 system. A harmonious coexistence of oxidation and reduction mechanism was proposed.
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ACS Applied Nano Materials
·
2019
·
IF 0
Zhuojun Wei,Yangguang Li,Liguang Dou,Muhammad Ahmad,Hui Zhang
A series of bi-transition-metal hybrids Cu3–xNixAl-LDH/rGO (x = 2.5, 2.0, 1.5) were synthesized via a facile preadjusted pH citric-acid-assisted coprecipitation method. Systematic characterizations suggest that the hybrids show honeycomb-like nanosheets array morphology with ultrathin CuNiAl-LDH nanosheets (∼72.3 × 4.2 nm) staggered vertically grown on both sides of rGO substrate. The Cu3–xNixAl-LDH/rGO hybrids present a remarkably superior reduction performance for 4-nitrophenol (4-NP) than pure LDH samples. Particularly, Cu1Ni2Al-LDH/rGO shows the best activity with kapp = 3.4 × 10–2 s–1, ∼1.7-fold of Cu1Ni2Al-LDH. It is worth noting that the activity of this bitransition metals hybrid Cu1Ni2Al-LDH/rGO is 37% higher than our previously reported monotransition metal hybrid Cu1Mg2Al-LDH/rGO (kapp = 2.5× 10–2 s–1). These findings can be ascribed to (1) highly dispersed ultrasmall Cu2O nanoparticles (∼3.8 nm) instantaneously formed via in situ reduction of atomic-level dispersed Cu2+ ions in LDH layer lattices of the Cu3–xNixAl-LDH hybrids beneficial from clear isolation and stabilization effect of Ni–OH; (2) strong promotion of the doping of atomic-level dispersed Ni2+ ions in LDH and possible Cu2O–Ni–OH(CuNiAl-LDH)–rGO three-phase synergistic effect; and (3) enhanced adsorption capacity for reactants upon π–π stacking and the unique honeycomb-like nanosheet array morphology. The series of hybrids exhibit not only excellent reduction properties for varied aromatic nitro compounds but also good decolorization ability for anionic azo dyes. Further fixed bed experiments reveal that Cu1Ni2Al-LDH/rGO can efficiently decolorize the mixed solution of 4-NP and methyl orange at high flow rate (8 mL/min) with TOF of 1.68 × 10–3 s–1, suggesting great application potential of the as-obtained nanosheet array bi-transition-metal hybrids for water remediation.
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shuangyu Xiong,Luminita Marin,Lian Duan,Xinjian Cheng
Nitroaromatic compounds, especially 2, 4, 6-trinitrophenol, have strong biological toxicity and explosive risks, so the detection of 2, 4, 6-trinitrophenol exhibit importantly practical and scientific significance. In this work, three fluorescence functionalized chitosan CNS 3 , CNS 4 and CNS 5 were prepared using chitosan as matrix. When 2, 4, 6-trinitrophenol (TNP) and/or p-nitrophenol (4-NP) was present in spot, these fluorescent chitosan sensors produced notable fluorescence quenching. It renders the chitosan sensing ability to detect TNP and 4-NP selectively and sensitively. The sensing mechanism is investigated as well. When introduced electron-rich moieties to the fluorescent chitosan, the sensitive detecting ability could be obtained. Excellent recognition ability could reach as low as 0.28 μM. The fluorescence fictionalization cause slight influence to the gel performance of chitosan.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Ting-Ting Li,Wen-Cheng Liu,Jiang Zhu,Yun-Huang Yang,Chao Ma,Chen Lu,Kun-Xiao Zhang
Green lacewing Chrysopa pallens (Rambur) is a general predator of many agricultural pests and plays a pivotal role in reducing crop damage by managing insect pest populations. Odorant binding proteins (OBPs) in insects can sense the semiochemicals in the environment and initiate the delivery of signals to their receptors. However, no Chrysopa pallens OBP (CpalOBP) structure has been reported yet, and their corresponding candidate semiochemicals are still largely unknown. Here, we reported the structure of CpalOBP4 solved with X-ray diffraction and showed its potential ligands. Our results showed that CpalOBP4 has a classical OBP structure with six α-helices and three disulfide bridges, and it can bind with farnesene , 2-tridecanone, cis-3-hexenyl hexanoate, nerolidol and farnesol through a central hydrophobic cavity. Our molecular docking results showed that Met31, Met78, Leu98, Phe141, Leu142 and Pro143 in the hydrophobic cavity were the key residues mediating the interaction of CpalOBP4 with farnesene , 2-tridecanone and cis-3-hexenyl hexanoate, which was further proven by the results that mutations of these residues led to significantly reduced binding affinities of CpalOBP4 for these ligands. Our study provides useful information for the further investigation of the biological function of CpalOBP4 as well as important cues for improving biological control in agriculture.
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ChemCatChem
·
2018
·Vol. 10
·期 18
·4143-4153
·
IF 4.674
Xinyu Wang,Jing Lu,Yunlong Zhao,Xiaopeng Wang,Zhang Lin,Xueming Liu,Ronglan Wu,Chao Yang,Xintai Su
Graphical SuperHUMATE performance : A novel catalyst, carbon anchored nickel/heazlewoodite nanoparticles (Ni/Ni 3 S 2 @C nanosheets), was fabricated by facile pyrolysis of solid precursor with potassium humate as carbon source and sulfur source. The Ni/Ni 3 S 2 @C nanocatalyst exhibited superior catalytic performance towards 4-nitrophenol reduction with excessed sodium borohydride as reactant, which the K /m value up to 166 s −1 g −1 . A facile molten salt method was utilized for the synthesis of carbon anchored nickel/heazlewoodite nanoparticles (Ni/Ni 3 S 2 @C nanosheets) with potassium humate as the carbon and sulfur source and NaCl as template. The morphology, particle size and crystallinity of the products were characterized by various techniques containing TEM, FE-SEM and XRD. Furthermore, the mesoporous Ni/Ni 3 S 2 @C own easy accessibility of active sites and high surface area (149.04 m 2 g −1 ). Thus, the as-prepared Ni/Ni 3 S 2 @C exhibited prominent performance for catalytic reduction of 4-nitrophenol (4-NP). Catalytic reduction of other nitrophenols (NPs) were also tested which can prove that the catalyst own selectivity on reduction of NPs. For durability, the property of the catalyst does not decrease obviously after five cycles. More significant correlations concerning effect of activation parameters ( E a =37.21 kJ mol −1 ) on 4-NP reduction were scrutinized and discussed. Hence, we provide a facile method for fabrication of metal/metal sulfide@C which own better dispersity, small dimension and excellent catalytic performance for further studies.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2018
·Vol. 22
·期 8
·2405-2412
·
IF 2.509
Mo Zunli,Niu Xiaohui,Gao Huhu,Li Zhenliang,Meng Shujuan,Guo Ruibin
A novel and simple chiral sensing platform had been successfully fabricated by means of amidation reaction between 3, 4, 9, 10-perylenetetracarboxylic acid (PTCA) and chitosan (CS) to form 3, 4, 9, 10-perylenetetracarboxylic acid–chitosan (PTCA–CS) composite film. Since CS has chiral center and PTCA has excellent electrical conductivity, the PTCA–CS composite modified glassy carbon electrode (PTCA–CS/GCE) could be treated as an effective electrochemical chiral sensor and applied for chiral discrimination of tryptophan (Trp) enantiomers theoretically. PTCA–CS composite was characterized by Fourier transform infrared (FTIR) spectroscopy and cyclic voltammetry (CV). When the prepared chiral sensing interface interacted with tryptophan isomers, a higher selectivity was received from D-Trp by differential pulse voltammetry (DPV). It indicated that the PTCA–CS/GCE can be treated as an electrochemical chiral sensor for the discrimination of Trp enantiomers. Further study demonstrated that the peak currents were linearly increased with the increasing percentage of L-Trp of Trp racemic mixture. Furthermore, the enantioselective interaction of the PTCA–CS/GCE was systematically studied by other experimental factors, such as the incubation time and acidity.
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POLYMER
·
2018
·
IF 3.483
Miaojun Xu,Kun Ma,Dawei Jiang,Jiaoxia Zhang,Min Zhao,Xingkui Guo,Qian Shao,Evan Wujcik,Bin Li,Zhanhu Guo
The effects of phosphazene based epoxy resin hexa-[4-(glycidyloxycarbonyl) phenoxy]cyclotriphosphazene (CTP-EP) as a synergistic agent on fire retardancy, thermal and mechanical properties of polyamide 6/aluminum diethylphosphinate (PA6/AlPi) composites were investigated. AlPi and CTP-EP presented an obviously synergistic flame retardant effect for the PA6 matrix. The PA6/AlPi/CTP-EP composites with a thickness of 1.6 mm successfully passed UL-94 V-0 rating with the limiting oxygen index value of 31.0% when the loading amount of AlPi/CTP-EP (mass fraction of 97/3) was 11 wt%. The incorporated CTP-EP improved the complex viscosity of PA6/AlPi/CTP-EP composites due to the chain extending reaction between the epoxide groups of CTP-EP and the terminal groups of PA6 matrix. The CTP-EP stimulated the formation of more sufficient and compact char layer during combustion. The higher melt viscosity and more compact char layer of PA6 composites further inhibited the volatilization of flammable gases, thus the ignition time of PA6/AlPi/CTP-EP composites was prolonged and the released heat was reduced. The introduction of CTP-EP simultaneously enhanced the flame retardant and mechanical properties of PA6/AlPi/CTP-EP composites compared with that of PA6/AlPi composites.
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BIOCHEMICAL PHARMACOLOGY
·
2018
·
IF 4.235
Binbin Sun,Zeyu Zhong,Fan Wang,Jiong Xu,Feng Xu,Weimin Kong,Zhaoli Ling,Nan Shu,Ying Li,Tong Wu,Mian Zhang,Liang Zhu,Xiaodong Liu,Li Liu
Skeletal muscle accounts for approximately 75% of glucose disposal in body and statins impair glucose metabolism. We aimed to investigate the effect of atorvastatin on glucose metabolism in C2C12 cells. Glucose metabolism and expression of glucose transporter 4 (GLUT4) and hexokinase II (HXKII) were measured following incubation with atorvastatin or pravastatin . Roles of cholesterol in atorvastatin-induced glucose metabolism impairment were investigated via adding cholesterol or mevalonic acid and confirmed by cholesterol depletion with methyl-β-cyclodextrin. Hypercholesterolemia mice induced by high fat diet (HFD) feeding, orally received atorvastatin (6 and 12 mg/kg) or pravastatin (12 mg/kg) for 22 days. Results showed that atorvastatin not pravastatin concentration-dependently impaired glucose consumption, glucose uptake and GLUT4 membrane translocation in C2C12 cells without affecting expression of HXKII or total GLUT4 protein. The atorvastatin-induced alterations were reversed by cholesterol or mevalonic acid. Cholesterol depletion exerted similar impact to atorvastatin, which could be alleviated by cholesterol supplement. Glucose consumption or GLUT4 translocation was positively associated with cellular cholesterol levels. In HFD mice, atorvastatin not pravastatin significantly increased blood glucose levels following glucose or insulin dose and decreased expression of membrane not total GLUT4 protein in muscle. Glucose exposure following glucose or insulin dose was negatively correlated to muscular free cholesterol concentration. Expression of membrane GLUT4 protein was positively related to free cholesterol in muscle. In conclusion, atorvastatin impaired glucose utilization in muscle cells partly via inhibiting GLUT4 membrane translocation due to inhibition of cholesterol synthesis by atorvastatin, at least, partly contributing to glucose intolerance in HFD mice.
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CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Nianbing Zhong,Ming Chen,Yihao Luo,Zhengkun Wang,Xin Xin,Bruce E. Rittmann
We present a novel UV–visible-light photocatalytic optical hollow-fiber in which a hollow optical fiber (HOF) and TiO 2 -based composite are coupled for enhancement of photocatalytic activity toward degradation of 4-chlorophenol (4-CP). A HOF was coated with a TiO 2 -based composite composed of Er 3+ :YAlO 3 /SiO 2 /TiO 2 (EYST) and acting as the photocatalytic element. The EYST coating showed good light-inducing capacity at the fiber-coating interface and high photocatalytic activity driven by UV–visible light. The photocatalytic activity of the fabricated photocatalytic HOFs was investigated for degradation of 4-CP in aqueous solution in the wavelength spectrum of 360–780 nm. Examined were the effects of the EYST coating gradient and reactor operating conditions (pH and temperature) on the degradation of 4-CP. The EYST-coated HOF significantly enhanced the 4-CP degradation, dechlorination, and mineralization, compared to pure TiO 2 -coated HOF and EYST-coated solid optical fibers. Specifically, high photocatalytic activity was obtained when the EYST coating thicknesses at the HOF’s incident end and HOF’s hemispherical end were 10 and 160 μm, the pH was 6.0, and temperature was 55 °C.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2018
·Vol. 20
·期 38
·24796-24806
·
IF 3.906
Chensheng Ma,Chris Tsz-Leung Chan,Ruth Chau-Ting Chan,Allen Ka-Wa Wong,Bowie Po-Yee Chung,Wai-Ming Kwok
Apart from being an analogue of the prototype for photoinduced intramolecular charge transfer (ICT), 2-ethylhexyl 4-dimethylaminobenzoate (EHDMABA) is also one of the earliest patented and most commonly used sunscreen components. There is, however, little documented information about the photophysics and factors affecting the photophysics of this molecule. Such information is of importance for both the understanding of the ICT reaction and assessing the underlying process of photoprotection, especially in view of the “sunscreen controversy” that has arisen from the contrasting in vivo vs. in vitro photobiological results on this and related UV filters. We report herein a femtosecond broadband time-resolved fluorescence (fs-TRF), complemented by transient absorption (fs-TA) to allow a full probe of the excited state cascades for EHDMABA and two of its derivatives in solvents of varied properties. The results provide direct evidence for a nearly solvent independent inner sphere ICT reaction occurring on the sub-picosecond time scale, and an ensuing solvent dictated deactivation of the ICT state. The ICT state in the aprotic solvent acetonitrile decayed solely through the intrinsic intersystem crossing (ISC) to produce a potentially harmful triplet excited state. In the protic solvent, the solvation and formation of ICT-induced solute–solvent hydrogen (H)-bonding opened the originally inaccessible internal conversion (IC) channel of the ICT state, leading to the rapid reformation of the ground state molecule with a unitary efficiency in the aqueous solution. This H-bonding-mediated IC restrained or eliminated the intrinsic ISC, providing a mechanism at the molecular level for the benign dissipation of the electronic excitation. The precise rate of IC was observed to vary with the alkoxy substituent and its efficiency was affected by the H-bonding capacity of the solvent. The findings of this work demonstrate the pivotal role of the microenvironment and the direct participation of solvent molecules through H-bonding in drastically altering the nonradiative dynamics and promoting or inhibiting photostability and photoprotection. This may assist in developing next-generation UV filters and help in improving formulation design for the optimal efficacy of sunscreen products. The pronounced H-bonding-induced fluorescence quenching and variation in the fluorescence wavelength imply that these molecules may also serve as a sensitive fluorescence probe for the H-bonding properties of the microenvironment.
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