Antioxidants
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2024
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IF 6
Xiao Xiao,Feiyu Yang,Yuling Huang,Shaohui Liu,Zhenhua Hu,Shanggao Liao,Yuanyuan Li
Numerous approaches targeting hepatic stellate cells (HSCs) have emerged as pivotal therapeutic strategies to mitigate liver fibrosis and are currently undergoing clinical trials. The investigation of herbal drugs or isolated natural active compounds is particularly valuable, due to their multifaceted functions and low risk of side effects. Recent studies have hinted at the potential efficacy of verbascoside (VB) in ameliorating renal and lung fibrosis, yet its impact on hepatic fibrosis remains to be elucidated. This study aims to evaluate the potential effects of VB on liver fibrosis by assessing its ability to inhibit HSC activation. VB demonstrated significant efficacy in suppressing the expression of fibrogenic genes in activated LX-2 cells. Additionally, VB inhibited the migration and proliferation of these activated HSCs by scavenging reactive oxygen species (ROS) and downregulating the AMPK pathway. Furthermore, a biosafe reverse microemulsion loaded with VB (VB-ME) was developed to improve VB’s instability and low bioavailability. The optimal formulation of VB-ME was meticulously characterized, revealing substantial enhancements in cellular uptake, ROS-scavenging capacity, and the suppression of HSC activation.
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PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Yandai Lin,Yanxi Yang,Xueli Ren,Zhe Liu
Exogenous electrical stimulation has attracted considerable attention due to the advantages of microelectric induction and subsequent biological effects such as actin reorganization and reactive oxygen species (ROS) generation. Herein, an injectable hydrogel of BPR-ARG@Gel (BAG) with pyroelectric BPR nanoparticle loading and l-arginine (ARG) introduction was fabricated for advanced cancer therapy in vivo. Due to the photothermal effect, the holes and electrons in BPR nanoparticles were separated to produce an open-circuit voltage and consequently catalyze water H2O to generate toxic superoxide (•O2–) and hydroxyl radicals (•OH). These ROS substances further oxidize ARG to produce NO for synergistic tumor treatments. The mice experiments indicated that the employment of BAG hydrogel incorporation with a near-infrared laser downregulated the heat shock protein and recruited immune cells with 5-fold-enhanced expression of proinflammatory cytokines of interferon-γ. It was also noteworthy that the injectable hydrogel of BAG substantially induced the generation of reactive oxygen/nitrogen species (ROS/RNS) with reliable biosafety and strong tumor inhibition. Overall, these findings have provided potentially new inspirations and a feasible strategy to translate this multifunctional hydrogel toward tumor therapy in a pyroelectric stimulation manner.
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高影响因子
Surfaces and Interfaces
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2025
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IF 6.3
Baiwei Ma,Xuanyu Lin,Tiaotiao Zhu,Xiaofei Zheng,Juanfang Zhu
s Bacterial infections pose a threat to human health worldwide. Developing antibiotic-free strategies or integrating multiple bactericidal methods is urgently needed for efficient sterilization. Covalent organic frameworks (COFs) have attracted considerable attentions for killing bacteria using photodynamic and photothermal therapy methods, which prevent drug resistance. In this study, a donor–acceptor (D–A)-type COF anchored with pyridinium and quaternary ammonium, named BPDS-COF, was developed to achieve ternary synergistic antibacterial effects. The pyridinium cations generated in situ not only sterilize but also attract and accommodate electrons to thus promote the photoelectric generation of ROS. Additionally, the quaternary ammonium salts provide good antibacterial activity. The simultaneous presence of pyridinium and quaternary ammonium can enhance the hydrophilicity and bacterial adsorption and improve adsorption for oxygen to more easily generate ROS, which are beneficial to sterilization. Moreover, BPDS-COF has the potential to be used as an oral antibacterial dental filler and implant coating material. This ternary synergistic antibacterial strategy could inspire the design of other multifunctional antimicrobial COFs.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Liyuan Yu,Jianqin Peng,Qun Han,Wanxin Huang,Yijie Jiang,Yongming Ruan,Xia Liu,Gesmi Milcovich,Xuexiang Weng
Dendritic mesoporous silica nanoparticles (DMSNs) have emerged as promising nanocarriers due to their unique three-dimensional structure and tunable pore characteristics. This study investigates the potential of DMSNs to deliver thyme essential oil (TEO) for enhanced antifungal activity against Fusarium oxysporum (F. oxysporum), a major plant pathogen. DMSNs were successfully synthesized and characterized, followed by the encapsulation of TEO within their porous structure. The resulting TEO@DMSNs composites exhibited significant antifungal activity against F. oxysporum, with inhibition rates reaching 70%, indicating an effective crop protection with a dose-dependent effect. The enhanced antifungal efficacy of TEO@DMSNs compared to free TEO is attributed to the sustained release of TEO and the synergistic effect of the nanocarrier itself. In-depth mechanism investigations revealed that TEO@DMSNs likely disrupted the fungal cell membrane, leading to leakage of cellular contents and ultimately cell death. Moreover, DMSNs and TEO@DMSNs were found to be safe for plant growth, demonstrating their potential as environmentally friendly antifungal agents. This study provides valuable insights into the design and development of advanced nanocarriers for targeted drug delivery and disease management in agriculture.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Meng Han,Jincheng Tang,Haiyue Zhao,Yachao Sheng,Ziang Li,Wenxiao Shi,Jiahao Wang,Chao Ma,Kun Xi,Yong Gu,Liang Chen
Management of dura tear during spine surgery remain challenging due to the complex focal environment. Current sealants could hardly independently repair dura tear while simultaneously regulate epidural inflammation and fibrosis induced by oxidative stress. A bioadhesive hydrogel sealant with active reactive oxygen species (ROS)-scavenging potential for suppression of epidural inflammation and fibrosis was designed to provide a versatile tool for spine surgeon. In order to achieve such goal, tannic acid-functionalized black phosphorus nanosheets with better ambient stability were incorporated and integrated into network of hydrogel sealant. Such integration was found to bring improved mechanical strength and sealing performance as evidenced by higher burst pressure and effective sealing of dura tear under in vivo environment. Moreover, instead of merely acting as a physical sealing barrier, the hydrogel sealant was also found with active ROS-scavenging capability. Effective inhibition on oxidative stress-mediated epidural inflammation and fibrosis was observed with employment of hydrogel sealant as revealed by both in vitro and in vivo studies. The proposed hydrogel sealant could provide a potential candidate for sealing of dura tear, and may hopefully provide a promising strategy for prevention of epidural fibrosis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Zhiheng Luo,Jianxiong Ma,Yan Wang,Yuhan Du,Yujie Liu,Wei Zhang,Yuxiao Lai,Xinlong Ma
The management of bone defects, particularly those with irregular geometries resulting from osteoporotic fractures, remains fraught with challenges. Microspheres have emerged as a promising vehicle for tissue engineering, distinguished by their controlled release, safety, and ease of application. Various bioactive components are integrated into microspheres to improve their performance. Metal-organic frameworks, formed from metal ions interconnected by organic ligands, are increasingly utilized in tissue engineering. Specifically, magnesium-based MOFs are notable for their broad applicability; Mg 2+ ions are instrumental in bone reconstruction and repair, facilitating osteogenesis, angiogenesis, antibacterial effects, and anti-inflammatory properties. Mg-MOF was synthesized using magnesium chloride and gallic acid, and it was incorporated into gelatin microspheres to create Gel@Mg-MOF composite microspheres. Leveraging gelatin's biocompatibility, controlled release, and biodegradability, the composites' biocompatibility was evaluated through toxicity and adhesion assays. Moreover, the osteogenic and angiogenic potentials of the Gel@Mg-MOF microspheres were assessed, alongside their capacity for ROS scavenging. Results suggest that controlled Mg 2+ release from Gel@Mg-MOF microspheres promotes osteogenic activity in RBMSCs and enhances angiogenic potential in HUVECs. Additionally, the gallic acid-containing composite microspheres exhibited antioxidative properties. Collectively, the findings suggest that Gel@Mg-MOF microspheres could provide effective support for bone defect repair, with potential for clinical deployment.
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CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Xinrang Zhai,Honghua Hu,Miner Hu,Shunxian Ji,Tao Lei,Xiaozhao Wang,Zhiqiang Zhu,Wei Dong,Chong Teng,Wei Wei
Diabetic wound infection brings chronic pain to patients and the therapy remains a crucial challenge owing to the disruption of the internal microenvironment. Herein, we report a nano-composite hydrogel (ZnO@HN) based on ZnO nanoparticles and a photo-trigging hyaluronic acid which is modified by o-nitrobenzene (NB), to accelerate infected diabetic wound healing. The diameter of the prepared ZnO nanoparticle is about 50 nm. X-ray photoelectron spectroscopy (XPS) analysis reveals that the coordinate bond binds ZnO in the hydrogel, rather than simple physical restraint. ZnO@HN possesses efficient antioxidant capacity and it can scavenge DPPH about 40 % in 2 h and inhibit H 2 O 2 >50 % in 8 h. The nano-composite hydrogel also exhibits satisfactory antibacterial capacity (58.35 % against E. coli and 64.03 % against S. aureus for 6 h). In vitro tests suggest that ZnO@HN is biocompatible and promotes cell proliferation. In vivo experiments reveal that the hydrogel can accelerate the formation of new blood vessels and hair follicles. Histological analysis exhibits decreased macrophages, increased myofibroblasts, downregulated TNF-α expression, and enhanced VEGFA expression during wound healing. In conclusion, ZnO@HN could be a promising candidate for treating intractable infected diabetic skin defection.
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Nanoscale
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2024
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IF 5.8
Yong Li,Jing Wang,Tao Zhu,Ying Zhan,Xiaoli Tang,Jianying Xi,Xiaohui Zhu,Yong Zhang,Jinliang Liu
Catalytic generation of toxic hydroxyl radicals (˙OH) from hydrogen peroxide (H2O2) is an effective strategy for tumor treatment in chemodynamic therapy (CDT). However, the intrinsic features of the microenvironment in solid tumors, characterized by limited H2O2 and overexpressed glutathione (GSH), severely impede the accumulation of intracellular ˙OH, posing significant challenges. To circumvent these critical issues, in this work, a CaO2-based multifunctional nanocomposite with a surface coating of Cu2+ and L-buthionine sulfoximine (BSO) (named CaO2@Cu–BSO) is designed for enhanced CDT. Taking advantage of the weakly acidic environment of the tumor, the nanocomposite gradually disintegrates, and the exposed CaO2 nanoparticles subsequently decompose to produce H2O2, alleviating the insufficient supply of endogenous H2O2 in the tumor microenvironment (TME). Furthermore, Cu2+ detached from the surface of CaO2 is reduced by H2O2 and GSH to Cu+ and ROS. Then, Cu+ catalyzes H2O2 to generate highly cytotoxic ˙OH and Cu2+, forming a cyclic catalysis effect for effective CDT. Meanwhile, GSH is depleted by Cu2+ ions to eliminate possible ˙OH scavenging. In addition, the decomposition of CaO2 by TME releases a large amount of free Ca2+, resulting in the accumulation and overload of Ca2+ and mitochondrial damage in tumor cells, further improving CDT efficacy and accelerating tumor apoptosis. Besides, BSO, a molecular inhibitor, decreases GSH production by blocking γ-glutamyl cysteine synthetase. Together, this strategy allows for enhanced CDT efficiency via a ROS storm generation strategy in tumor therapy. The experimental results confirm and demonstrate the satisfactory tumor inhibition effect both in vitro and in vivo.
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JOURNAL OF CONTROLLED RELEASE
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2024
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IF 10.5
Jianwei Wang,Xiaojia Lv,Ying Li,Haiqiang Wu,Meiwan Chen,Hua Yu,Jianwei Wu,Chenyang Li,Wei Xiong
Intestinal mucosal barrier loss is responsible for the chronic and recurrent ulcerative colitis. Myosin light chain kinase (MLCK) is a potential therapeutic target of the intestinal mucosal barrier dysfunction. Here, we developed a reactive oxygen species (ROS)-sensitive hydrogel (ATG-CS-Gel) derived from a diselenide-bridged arctigenin (ATG) and chitosan (CS) conjugate, with the aims of targeting to inflamed mucosa and modulating MLCK. Our results demonstrated that ATG-CS-Gel achieved ROS-responsive release and significantly inhibited ROS production and mitochondrial depolarization in the Caco-2 and HT-29/MTX-E12 cells under H 2 O 2 -induced stress conditions. Compared with normal tissues, orally-administrated ATG-CS-Gel preferentially adhered to the inflamed mucosa for 24 h, which was attributed to the adhesion between CS and mucin. Therapeutically, ATG-CS-Gel reduced inflammatory symptoms, accelerated intestinal mucosal healing, scavenged excessive ROS, reshaped intestinal flora, and eventually achieved much better therapeutic efficacy in DSS-induced colitis mice when compared to 5-aminosalicylic acid. Moreover, ATG-CS-Gel was demonstrated to reverse intestinal mucosal barrier loss by blocking MLCK activation and maintaining tight junction expression. In summary, this study highlights the potential of MLCK modulation in the restoration of intestinal mucosal barrier using ATG-CS-Gel. The development of ATG-CS-Gel represents a novel and promising strategy for the treatment of ulcerative colitis.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2025
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IF 5.2
Xiaohui Jia,Yuhe Dong,Jihui Lu,Zhenyuan Yang,Ran Xu,Xiang Zhang,Jingyi Jiao,Zixuan Zhang,Yixuan Lin,Fuhao Chu,Penglong Wang,Tian Zhong,Haimin Lei
On-demand responsive hydrogels are a promising solution for effective wound management as they can adjust their properties in response to changes in the wound environment, allowing them to provide tailored support for the healing process. However, the conventional hydrogels may not fully meet the diverse demands of the intricate healing process. Herein, a novel glycyrrhizic acid (GA) based self-assembly hydrogel coordinated with copper and polyphenol (GCP hydrogel) was developed to exhibit triggered release behavior in response to the microenvironment. The GCP hydrogel coordinated with copper and protocatechuic acid (PA) and self-assembled with GA, also exhibits enzyme-like properties by mimicking the cascade process of superoxide dismutase (SOD) and catalase (CAT), effectively scavenging reactive oxygen species (ROS). Furthermore, the on-demand release of Cu 2+ at different stages of the wound healing process can not only enhance the antibacterial ability of methicillin-resistant Staphylococcus aureus (MRSA) but also intelligently promote angiogenesis with outstanding biocompatibility. In addition, the GCP hydrogel effectively modulated the activity of macrophages in response to inflammatory stimuli, exhibiting remarkable anti-inflammatory abilities and promoting tissue regeneration. The multifunctional GCP hydrogel platform has the potential to create a dynamic microenvironment that is conducive to tissue regeneration, making it an ideal candidate for smart wound management.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
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IF 4.7
Zhen He,Xiangyun Tan,Ming Yuan,Liang Chen,Yan Meng,Qi Wang,Junjie Hu,Zhenpeng Qiu,Yuan Yang
Acute liver injury (ALI) is a prevalent form of hepatic disease associated with significant morbidity and mortality due to medical treatments, exposure to toxins or viral infections. Anethole trithione (ATT) is a heterocyclic sulfur compound recognized for its chemoprotective properties against cancer and drug-induced toxicity. This study aimed to evaluate the effectiveness of ATT in the treatment of ALI. The therapeutic effects of ATT on hepatic injury were evaluated in vivo by inducing ALI in mice through the administration of lipopolysaccharide (LPS) and D-galactosamine (D-Gal). Additionally, HepG2 and Huh7 cells exposed to LPS were utilized to investigate the underlying mechanisms in vitro. The results indicated that ATT significantly reduced the production of reactive oxygen species (ROS), mitigated oxidative stress-related biochemical markers, and inhibited hepatocyte apoptosis in vivo, resulting in marked improvement in ALI in the murine model. Mechanistic studies conducted both in vivo and in vitro demonstrated that ATT alleviates LPS/D-Gal-induced ALI by inhibiting ROS production and the activity of nuclear factor-kappa B (NF-κB). Collectively, these findings underscore the potential therapeutic benefits of ATT in the management of ALI.
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CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Hui Yan,Lietao Wang,Haoshuang Wu,Yongqi An,Yumei Qin,Zhen Xiang,Huining Wan,Yanfei Tan,Li Yang,Fanjun Zhang,Qing Jiang,Rifang Luo,Yunbing Wang
Unhealthy vascular tissue healing after stenting is primarily triggered by endothelial dysfunction and excessive smooth muscle cell proliferation, which is induced and aggravated by local thrombosis and excessive inflammation. Excessive inflammatory response is closely related to elevated concentrations of reactive oxygen species (ROS). Here, an environment-friendly coating strategy termed “Anti-fouling plus” with inflammatory self-regulation ability was proposed. Upon this, membrane-mimicking copolymer MA(PCLA) served as the antifouling coating, effectively inhibiting coagulation and inflammation during the early stage of implantation. Furthermore, a ROS-responsive prodrug was drawn into the coating to promote tissue healing. A molecular prodrug of thioketal-bearing dexamethasone was engineered to be released in a controlled and responsive manner in response to externally high levels of ROS. The released dexamethasone exhibited highly effective anti-inflammation properties after implantation. The combination of ROS-responsive prodrug and membrane-mimicking antifouling interface strategy intelligently integrated inflammation responsiveness and regulation, which could mediate a gentle vascular microenvironment and thus promote vascular remodeling. In vivo stent implantation studies demonstrated that the proposed “Anti-fouling plus” coating effectively inhibited neointima hyperplasia, modulated the inflammatory response and promoted endothelialization, offering promising surface modification approaches for vascular stents.
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