MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-10
·
IF 5.3
Dong Hui,Jiang Zhenlong,Chen Yanan,Han Huabo,Zhou Yanli,Wang Xiaobing,Xu Maotian,Liu Lantao
Hydroxyl radical (•OH) detection is pivotal in medicine, biochemistry and environmental chemistry. Yet, electrochemical method–specific detection is challenging because of hydroxyl radicals’ high reactivity and short half-life. In this study, we aimed to modify the electrode surface with a specific recognition probe for •OH. To achieve this, we conducted a one-step hydrothermal process to fabricate a CoZnMOF bimetallic organic framework directly onto conductive graphite paper (Gp). Subsequently, we introduced salicylic acid (SA) and methylene blue (MB), which easily penetrated the pores of CoZnMOF. By selectively capturing •OH by SA and leveraging the electrochemical signal generated by the reaction product, we successfully developed an electrochemical sensor Gp/CoZnMOF/SA + MB. The prepared sensor exhibited a good linear relationship with •OH concentrations ranging from 1.25 to 1200 nM, with a detection limit of 0.2 nM. Additionally, the sensor demonstrated excellent reproducibility and accuracy due to the incorporation of an internal reference. It exhibited remarkable selectivity for •OH detection, unaffected by other electrochemically active substances. The establishment of this sensor provides a way to construct MOF-modified sensors for the selective detection of other reactive oxygen species (ROS), offering a valuable experimental basis for ROS-related disease research and environmental safety investigations. Graphical
研究领域:
医学
·
生物化学
·
环境化学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 18
·期 1
·95-104
·
IF 3.4
Sun Xi,Liu Feng,Xue Xiao
Ensuring food safety necessitates rapid and reliable food contaminant detection methods. Here, we present a novel approach that integrates electrochemical sensors with machine learning to detect Sudan Red I in food. Our study showcases a modified stainless steel needle (SSN) electrode adorned with silver nanoparticles (AgNPs) and investigates three distinct convolutional neural network (CNN) architectures: Inception V1, ResNet-50, and SqueezeNet V1.1. This combination aims to predict the sensor response accurately. The AgNPs/SSN electrode displays heightened electrochemical activity towards Sudan Red I, offering a linear detection range of 0.1–20 μM and an exceptional 10 nM detection limit. Notably, the Inception V1 and ResNet-50 networks exhibit superior predictive capability compared to SqueezeNet V1.1. Our interdisciplinary approach establishes a rapid, sensitive, and dependable detection system for food contaminants. By fusing electrochemical sensors with machine learning, we propel the development of intelligent detection systems with significant implications for food safety enhancement. Graphical abstract
研究领域:
食品科学
·
分析化学
·
人工智能
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Zha Jiahao,He Chongjun,Chen Fangzhou,Wang Hongwei,Dong Biao,Liu Lijuan,Xia Mingjun,Deng Chenguang,Li Qian,Lu Yuangang,Chen Huiting
Oxide matrix red-emitting phosphors are deemed as excellent color converters for white light emitting diodes (WLEDs) and laser diodes (LDs). Manganese-doped MgAl 2 O 4 powder was synthesized by a solid-state reaction method at different sintering temperatures. Microstructure shows that grain size is mainly in the range of 0.2–5 μm, and grain agglomeration occurs with increased sintering temperature. XPS analysis indicates that the doped Mn ion exhibits a valence state of + 4 within the MgAl 2 O 4 matrix. The diffraction peak of the phosphors is shifted by the sintering temperature, which affects lattice constant. Upon excitation by 300 nm ultraviolet light, the samples emit asymmetric broadband red light within the range of 620–720 nm, attributed to Mn 4+ ion’s transition from 2 E g to 4 A 2g states. With the increasing temperature, the main emission peak shifts from 677 nm to 650 nm, ascribed to the change in energy level ( 2 E g ) resulting from the reduction of Al 2 O 3 phase. Crystal field theory confirmed that Mn 4+ ions are within a strong crystal field environment created by MgAl 2 O 4 matrix. By affecting particle size and crystallinity, the sintering temperature influences the fluorescence lifetime of the Mn 4+ ion. Notably, these red-emitting phosphors exhibits remarkable thermal stability as their emission intensity remains approximately at 58% of initial intensity even at elevated temperature (435 K). Consequently, Mn 4+ : MgAl 2 O 4 red-emitting phosphors with high thermal stability render them promising candidates for WLED applications.
研究领域:
材料科学
·
光学
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Peng Fei,Ai Xiangnan,Bu Xiaoyu,Zhao Zixuan,Gao Baoxiang
Mitochondrial membrane potential (MMP) is crucial for mitochondrial function and serves as a key indicator of cellular health and metabolic activity. Traditional lipophilic cationic fluorescence intensity probes are unavoidably influenced by probe concentration, laser intensity, and photobleaching, limiting their accuracy. To address these issues, we designed and synthesized a pair of fluorescence molecules, OR-C8 and SiR-BA, based on the Förster Resonance Energy Transfer (FRET) mechanism, for dual-modality visualization of MMP. OR-C8 anchors to the inner mitochondrial membrane through strong hydrophobic interactions, while SiR-BA is expelled from mitochondria when MMP decreases, thereby regulating the FRET process. During MMP reduction, the fluorescence intensity and lifetime of OR-C8 increase, while the fluorescence intensity of SiR-BA decreases. By combining changes in fluorescence intensity ratio and fluorescence lifetime, dual-modality visualization of MMP was achieved. This method not only accurately reflects MMP changes but also provides a novel tool for in-depth studies of mitochondrial function and related disease mechanisms, offering significant potential for advancing mitochondrial research and therapeutic development.
研究领域:
细胞生物学
·
生物物理学
·
荧光显微镜技术
·
分子生物学
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DOI
PubMed
高影响因子
ONCOGENE
·
2024
·
IF 6.9
Du Jiaying,Bai Dongsheng,Gu Chunyang,Zhao Jiawei,Zhou Chen,Wang Yuxiang,Zhao Yue,Lu Na
Hepatocellular carcinoma (HCC) stands as one of the most aggressively advancing and lethal malignancies. Sorafenib is presently endorsed as a primary therapy for advanced liver cancer, but its resistance presents a formidable challenge. Previous studies have implicated a connection between post-sorafenib discontinuation rebound and the development of drug resistance, yet the underlying mechanism remains elusive. In this study, we discerned that Sorafenib induced a senescent phenotype in HCC cells and caused a cleavage of ubiquitin-binding protein p62. Mechanistic studies establish that truncated p62 drives cellular senescence by promoting proteasome-dependent degradation of 4EBP1. Furthermore, truncated p62 induced specific ubiquitination of 4EBP1. Crucially, virtual drug screening uncovered that dacinostat inhibited cellular senescence by blocking sorafenib-induced p62 cleavage. In summary, our findings imply that truncated p62 from sorafenib cleavage promotes senescence via 4EBP1 degradation. The prevention of p62 cleavage could emerge as a crucial strategy for impeding the sorafenib-induced cellular senescence.
研究领域:
肿瘤学
·
分子生物学
·
药理学
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DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2023
·Vol. 52
·期 4
·2665-2675
·
IF 2.1
He Bangrong,Zou Zhengguang,Zhang Weijian,Jiang Xinyu,Mao Zheng
The Pechini sol–gel and solid-state methods were used to prepare La 0.7 Sr 0.3 Mn 0.95 Ni 0.05 O 3 specimens for analysis of their microscopic morphology, magnetic and magnetocaloric effect properties. The results showed that the average particle size of the sample made by the Pechini sol–gel method was 127.7 nm, smaller than 444.6 nm using the solid-state method . The Curie temperature ( T C ) of Pechini sol–gel (S–G) and solid-state (S-S) samples was 321.7 K and 334 K; the Δ S M was 4.15 J kg −1 K −1 and 3.11 J kg −1 K −1 under an applied magnetic field of 5 T. The samples were all second-order phase transition materials, indicating that the phase transition order was independent of the synthesis method. The T C of the S–G sample shifted to a lower temperature and the Δ S M was significant, which is desired for magnetic refrigeration. This shows that the synthesis path strongly impacts the morphology and physical properties, explaining the physical mechanism of these property changes in detail. Graphical Abstract
研究领域:
材料科学
·
物理学
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DOI
高影响因子
TRIBOLOGY LETTERS
·
2020
·Vol. 68
·期 3
·1-14
·
IF 2.566
Liu Chenxu,Meng Yonggang,Tian Yu
Abstract The active control of friction in oil-based lubricants was realized in the present study with the use of MoS 2 particle additives and the application of an electric field. By modifying the surface charging state of the MoS 2 particles, the dependence of potential-controlled boundary lubrication behavior on the electrical properties of the particles was demonstrated. For a diethyl succinate lubricant containing negatively charged MoS 2 particles, the coefficient of friction (COF) was reduced by 60–70% when a positive potential was applied to a lower friction pair of copper plates. After modification with poly(diallyldimethylammonium chloride), the particles were positively charged, and the COF was reduced with the application of a negative potential. The mechanisms underlying the potential control of the COF were investigated by observing the distributions of the particle additives and characterizing the tribofilms formed at different potentials. Most of the charged particles were locally concentrated near the opposite pole, and this was reversed when the electric field changed. For locally high concentrations of MoS 2 particles, a MoS 2 /MoO x tribofilm with a thickness of 100–500 nm and a loose structure formed on the lower friction pair, which significantly decreased the shear force during the friction process. Graphical Abstract
研究领域:
材料科学
·
机械工程
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 30
·23515-23523
·
IF 2.779
Yao Zhongxiang,Bi Gang,Yin Juxin,Chen Zeyu,Cai Chunfeng
Due to the narrow band gap and high quantum confinement effects, Group IV–VI PbSe semiconductor materials have wide applications in infrared detectors. The morphology and size of nanomaterials affect the physical and chemical properties of PbSe semiconductor materials. Here, we developed a synthesized and grew PbSe-branched, I-shaped, and L-shaped nanorods method with uniform distribution by modulating the reaction environment. The results suggested that the arm length of the nanorods increased along the < 100 > axes with reaction time. The directional attachment of nanoparticles along the {100} plane was enhanced by appropriately increasing the reaction temperature. NH 4 Cl could promote the orientational attachment of PbSe nanocrystals along the {100} planes to form I-shaped and L-shaped nanorods. The PbSe-branched nanorod arms were uniformly distributed between 4.2 and 21 nm corresponding to an absorption spectrum between 1086 and 1574 nm and photoluminescence between 1301 and 1621 nm. This indicates that the optical properties are tunable in the near-infrared. Therefore, this research expands the potential applications of PbSe nanostructures in tunable infrared lasers, bioimaging, and solar cells, among others.
研究领域:
材料科学
·
纳米技术
·
光学
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DOI
高影响因子
TRANSITION METAL CHEMISTRY
·
2019
·Vol. 44
·期 8
·779-787
·
IF 1.016
Li Laiming,Li Youxin,Assefa Aschenaki,Bao James J.
Platinum was immobilized on magnetic silica gel by means of boronic, nitric, carboxylic or sulfuric acid ligands to give four heterogeneous Pt nano-catalysts, designated as Fe 3 O 4 @SiO 2 -BA@Pt, Fe 3 O 4 @SiO 2 -NA@Pt, Fe 3 O 4 @SiO 2 -CA@Pt and Fe 3 O 4 @SiO 2 -SA@Pt, respectively. Particles of these mono-dispersible Pt catalysts were 10–20 nm in size and could be separated for recycling by means of a magnet. Fe 3 O 4 @SiO 2 -BA@Pt (0.174 mmol/g Pt) showed the best catalytic activity and selectivity, which were better than Speier’s catalyst. Its turnover numbers were up to 1.7 × 10 6 and 1.1 × 10 6 for hydrosilylation of 1-hexene or styrene, respectively. This material could also catalyze the hydrosilylation of a broad range of alkenes and alkynes as substrates and methyldichlorosilane or triethoxysilane as silanes. Similar yields of 1-hexyl-methyldichlorosilane at the first and eighth runs (96.5% and 95.2%, respectively), together with a final Pt content of 0.171 mmol/g indicated the outstanding stability of Fe 3 O 4 @SiO 2 -BA@Pt under the catalytic reaction conditions.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 2
·1186-1197
·
IF 4.682
Li Yana,Li Yuqiang,Ji Yi,Wang Hong,Zhong Dingyong
Ga 2 O 3 is considered as a candidate for the preparation of solar-blind photodetectors due to its large direct band gap. Developing low-cost preparation methods of high-quality Ga 2 O 3 films is of importance for large-scale applications. In this work, we investigate the preparation of Sn doped β-Ga 2 O 3 films by using simple and cheap sol–gel method under ambient condition. It is found that Sn doping induces a growth mode transformation from \(\left( {\overline{2}01} \right)\) to \(\left( {\overline{3}11} \right)\) oriented texture on the c-plane sapphire substrates. With an optimal Sn proportion, high-quality β-Ga 2 O 3 films consisting of needle-like grains are observed. Compared with the undoped films, the Sn-doped films with needle-like grains obtained a low dark current of 0.76 nA, performed an on/off ratio as high as ~ 6000, responsivity, R of 164 mA/W and D* of 3.21 × 10 12 Jones at a bias voltage of 30 V in the configuration of metal–semiconductor–metal solar-blind deep ultraviolet detectors. This is the highest on/off ratio of Ga 2 O 3 solar-blind photodetectors fabricated by sol–gel method reported up to now and has the potential to be applied in practical use. Graphical abstract
研究领域:
材料科学
·
半导体物理
·
光电技术
✓ 已验证
DOI
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2026
·
IF 8.4
Li Hai-bo,Liu Yong-hui,Liu Hai,Li Yuan,Le Qiu-min,Wang Fei-fei,Ma Lan,Liu Xing
The high relapse rate of drugs is currently a therapeutic dilemma in the treatment of substance use disorder (SUD). Emerging evidence from preclinical animal models demonstrates that pretreatment with selective dopamine D 2 receptor (D 2 R) antagonists prevents reinstatement of drug-seeking. However, the role of D 2 R downstream signaling in regulating relapse behavior remains unclear. In this study, we investigated the roles of G α i -protein- and β-arrestin-dependent D 2 R signaling pathways in cocaine-primed reinstatement using cocaine self-administration (SA) mouse model treated with biased ligands. We found that treatment of D 2 R G α i -protein antagonists, but not β-arrestin antagonists, significantly attenuated cocaine-primed reinstatement of drug seeking without affecting locomotor activity or anxiety levels. Administration of D 2 R G α i -protein antagonists, but not β-arrestin antagonists, increased cyclic adenosine monophosphate (cAMP) levels in the nucleus accumbens (NAc). Furthermore, treatment of D 2 R G α i -protein antagonists, but not β-arrestin antagonists, suppressed cocaine-induced neuronal activation in the NAc. Our results demonstrate that G α i -protein-dependent D 2 R signaling plays a crucial role in cocaine-primed reinstatement and suggest that D 2 R G α i -protein-biased ligands may be promising pharmacotherapeutic targets for SUD treatment.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 12.6
Chaoran Liu,Wenyu Zhang,Qingbin He,Xinyu Ma,Jianfeng Qiu,Runxiao Zheng,Hongjin Xue
Catalyzing the glucose cascade reaction to impair tumor cell energy metabolism represents a promising strategy for tumor therapy. However, the application of natural enzymes as therapeutic agents remains limited by various challenges. Although nanozymes with multi-enzyme activities, including glucose oxidase-like (GOx-like) activity, have been reported, their development often involves complex material combinations and cumbersome synthesis processes. Here, we develop a nanozyme with GOx-, peroxidase (POD)-, superoxide dismutase (SOD)-, and NADH oxidase (NOX)-mimic activities by simply controlling the AuPt alloy ratio. The optimal cascade activity was observed for the nanozyme at an Au: Pt proportion of 13:7. Density functional theory (DFT) calculations revealed that Au sites drive glucose dehydrogenation (GOx-like), while Pt sites facilitate hydroxyl radical (•OH) generation (POD-like). Both in vitro and in vivo data indicated that Au 13 Pt 7 nanozymes disrupt tumor redox/metabolic homeostasis by depleting glucose and generating cytotoxic •OH, and impairing mitochondrial function via NOX-like, thereby inducing cell apoptosis. Notably, apoptotic immunogenic cell death (ICD) induces antitumor immunity and suppresses tumor metastasis. This study presents an innovative strategy for engineering nanozymes with multi-enzyme catalytic capabilities while demonstrating the promising application of alloy-based nanozymes in synergistic metabolic-immunotherapy.
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产品关联
DOI
PubMed
高影响因子
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