TALANTA
·
2022
·
IF 6.556
Jingjing Shu,Wenli Xiong,Ran Zhang,Shanyun Ma,Kaiqiang Zhou,Xuwei Wang,Fen Yan,Da Huang,Jianhua Li,Yuanzi Wu,Jian'an He
In view of the biological significance and micro-heterogeneity of protein glycosylation for human health, specific enrichment of N -glycosylated proteins/peptides from complex biological samples is a prerequisite for the discovery of disease biomarkers and clinical diagnosis. In this work, we propose a “grafting-from” N-glycoprotein enriching method based on the in-situ growth of thermoresponsive polymer brushes from the N -glycosylated site of proteins. The initiator was first attached to the pre-oxidized glycan moieties by hydrazide chemistry , from which the thermoresponsive polymers can be grown to form giant protein-polymer conjugates (PPC). The thermosensitive PPC can be precipitated and separated by raising the temperature to above its lower critical solubility temperature (LCST). Mass spectrometry verified 210 N -glycopeptides corresponding to 136 N-glycoproteins in the rabbit serum. These results demonstrate the capability of the tandem thermoprecipitation strategy to enrich and separate N-glycoprotein/glycopeptide. Due to its simplicity and efficiency specifically, this method holds the potential for identifying biomarkers from biological samples in N -glycoproteome analysis.
研究领域:
生物化学
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分析化学
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蛋白质组学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Materials Express
·
2021
·Vol. 11
·期 7
·1092-1100
·
IF 0
Liu Mingyan,Lin Ling,Shao Miaomiao,Guo Jun,Zhang Qiang,Chang Caiwang
Defects within the oral mucosa can result from a variety of causes, including tumors and trauma. Adults have limited ability to regenerate after tissue loss or injury, meaning autografts to reconstruct defects are often required. Due to shortcomings associated to tissue transplantation,
the use of tissue engineering technology to reconstruct and regenerate oral tissues has gained attention. In this regard, HAM (human amniotic membrane) was utilized as a biological material for tissue engineering applications. However, its unfavorable properties have limited its use, and hybrid
scaffolds based on HAM have been investigated. Thus, in this study, a biomimetic hybrid scaffold composed of gelatin methacryloyl and decellularized human amniotic particles (GelMA-dHAP) was prepared and used as a tissue substitute to promote wound healing of the oral mucosa in rabbits. First,
the degradation and swelling characteristics of the scaffold were evaluated and found to be more desirable than those of GelMA alone. Then, the effects of the GelMA-dHAP stent on the proliferation and differentiation of human foreskin fibroblasts were studied in vitro using a cell counting
kit-8 (CCK8) and immunofluorescence, respectively. This showed that the hybrid scaffold could significantly promote fibroblast proliferation and differentiation in comparison to controls. Following these initial assessments and to better understand the underlying mechanism of wound healing,
in vivo histological and immunohistochemical studies were undertaken using a rabbit oral mucosa defect model. The results showed that GelMA-dHAP can promote angiogenesis and collagen expression within the oral mucosa. Importantly, the GelMA-dHAP scaffold was shown to assist wound healing
better than GelMA alone, demonstrating the potential of the hybrid scaffold in tissue engineering.
研究领域:
生物医学工程
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组织再生
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伤口愈合
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材料科学
✓ 已验证
DOI
American Journal of Translational Research
·
2021
·
IF 4.06
Lexiang Li,Yi Chen,Qiwei Fu,Haishan Wu,Yiqin Zhou,Jiahua Shao,Jun Wu,Yaguang Han,Qirong Qian
Background: Tissue engineering is widely applied to treat osteochondral damage in osteoarthritis (OA). However, the superposition of seed cells, material scaffolds, inducing factors, and microenvironmental factors limit their practical application. We intended to develop a novel tissue engineering method for improving the repairment of osteochondral damage and to discuss its effect on repairing osteochondral defects. Methods: The combined decellularization methods of physics, chemistry and enzymes were used to decellularize rabbit rib cartilage and articular cartilage, and rabbit decellularizated osteochondral composite scaffolds were prepared. The structure and organization of the scaffolds were analyzed. We extracted and identified infrapatellar fat pad stem cells (IPFP-SCs) from healthy rabbits and OA rabbit, which were different in viability, migration, osteogenic and chondrogenic differentiation. Finally, a variety of decellularizated bone cartilage composite scaffolds were loaded with rabbit IPFP-SC for in vitro and in vivo studies. Results: The decellularization effect was strong, and the organic ingredients were lost. The layered scaffold showed lower density, greater porosity, larger pore size and water absorption than the whole scaffold, but the mechanical properties of the two scaffolds were low. IPFP-SCs were successfully extracted, and the migration and cartilage ability of IPFP-SCs in OA group were weak. The decellularized scaffold showed a high biocompatibility. The structure and composition of osteochondral promoted osteogenic differentiation and chondrogenic differentiation of IPFP-SCs. Moreover, the decellularized extracellular matrix loaded with IPFP-SC had the strongest repairing effect. Conclusion: The decellularized extracellular matrix loaded with IPFP-SC showed a better repair effect on rabbit osteochondral defects.
研究领域:
组织工程
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骨科
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干细胞研究
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Qiangjun Kang,Guohui Liu,Changan Liu,Weina Ren,Yuqing Wang
Bone defects are common consequences of bone trauma. The application of biomaterials as tissue scaffolds combined with oxygenating agents, as bone regeneration promoters, is an interesting strategy to improve bone repair. The present study aimed to investigate the potential of a temperature-sensitive hydrogel containing 1-bromoheptafluorooctane in repairing rabbit bone defects and its possible mechanism of action. Bone defects were surgically created in the femoral condylar of New Zealand White rabbits. The animals were divided into three groups: A) treated with the temperature-sensitive hydrogel containing 1-bromoheptafluorooctane with oxygen release; B) treated with the temperature-sensitive hydrogel containing 1-bromoheptafluorooctane without oxygen release; and C) received no treatments. At four and 12 weeks post-operation, the specimens from all groups were collected. The new bone formation was studied by morphological observation, X-ray, Micro-CT, Masson’s trichrome, and CD31 immunohistochemical staining. At 12 weeks post-operation, the bone defects were completely healed in groups A and B, while a major bone defect was still present in group C. Micro-CT showed that TMD, BVF, Tb.Th and Tb.N in group A were significantly higher compared to the other groups at each time point post-operation. The SMI value of group A showed a significant difference from the other groups at 12 weeks post-operation. The number of micro-vessels in group A was significantly higher compared to the other groups at four and 12 weeks post-operation. The oxygenating temperature-sensitive hydrogel containing 1-bromoheptafluorooctane could effectively promote new bone formation while increasing tissue vascularization, highlighting a good clinical application prospect.
研究领域:
生物医学工程
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组织工程
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骨科
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生物材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
·
2023
·
IF 2.7
Gongyan Liu,Haitao Sun,Ce Liu,Liya Bai,Liping Yang,Wenxue Jiang,Shuxia Gao
This study was conducted to investigate the effects of different dietary fibre sources on growth performance, gastrointestinal tract development, caecal fermentation and bacterial composition in the caecal contents of rabbits. A total of 120 35-day-old weaned Minxinan black rabbits were divided into three groups and fed a diet composed of peanut straw powder (Group A), alfalfa powder (Group B) and soybean straw powder (Group C) as the main fibre source. The final body weight and average daily gain in Group B were higher than those in Group C, and the average daily feed intake and feed conversion ratio in Group A were lower than those in Group C ( p < 0.05). The relative weights of the stomach, small intestine and caecum of rabbits in Group C were higher than those in Groups of B and A, and the relative weights of the caecal contents in Group C were lower than those in Groups A or B ( p < 0.05). The pH value and propionic acid, butyric acid and valeric acid concentrations in the caecum of Group C were lower than those in the caecum of Groups A or B, and the concentration of acetic acid in the caecum was lower ( p < 0.05). The dominant microbes in the caecal contents of Minxinan black rabbits were Firmicutes, Bacteroidetes and Proteobacteria at the phylum level, and the number of species, Chao1 index and ACE index measured was different between the B–C and A–C groups ( p < 0.05). Different dietary fibre sources could affect the growth performance, gastrointestinal tract development and intestinal microbiota of rabbits, and the nutritional value of alfalfa powder was better than that of peanut straw and soybean straw.
研究领域:
动物营养
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微生物学
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胃肠生理学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Wen-Shuai Jiang,Wei Xin,Shuang Xun,Shao-Nan Chen,Xiao-Guang Gao,Zhi-Bo Liu,Jian-Guo Tian
A sensitive and selective optical biosensor based on reduced graphene oxide (RGO), which uses the polarization-dependent absorption effect of graphene under total internal reflection, is reported for determination of rabbit IgG. RGO sheets with the thickness of about 8.1 nm are fabricated by high temperature reduction and used as a sensing film, because of its strong polarization dependent absorption. This RGO-based optical sensor shows a satisfactory response to rabbit IgG with a minimum concentration of 0.0625 μg/ml. As a contrast, commercial SPR apparatus is used to investigate rabbit IgG with a minimum concentration of 0.3125 μg/ml. Moreover, with antigen–antibody binding, this sensor can also achieve label-free and real-time detection. Taking into account these factors, the RGO-based optical sensor may be a potential candidate for biosensing.
研究领域:
材料科学
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生物传感器
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光学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Fan Leng,Sai Lei,Bin Luo,Siyao Lv,Long Huang,Xulin Jiang
As a minimally invasive method, endovascular embolization has been an effective strategy for controlling bleeding and tumor treatment. Herein, carboxymethyl chitin embolic microspheres were prepared with the aqueous two-phase carboxymethyl chitin/polyethylene glycol system without using any crosslinking agents and thrombin-functionalized embolic microsphere named as [email protected] was made after covalent introduction of thrombin. The size of the microspheres can be adjusted from 5 to 500 μm. The data of in vitro and in vivo tests indicated that these microspheres possessed good degradability and biocompatibility. Meanwhile, [email protected] can significantly promote blood clotting and enhance the strength of the blood clots . More importantly, [email protected] displayed better embolization effect than that of the commercial available Gelfoam Alicon® and polyvinyl alcohol-based embolic microspheres CalliSpheres® in rat femoral vein and rabbit ear artery embolization models. Therefore the size-tunable and biodegradable thrombin-functionalized carboxymethyl chitin microspheres [email protected] possess great potential for effective hemostasis and endovascular embolization.
研究领域:
生物医学工程
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药剂学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Yutao Ma,Zhihua Li,Yucheng Luo,Yao Chen,Le Ma,Xiaoya Liu,Jingyu Xiao,Man Huang,Yingnan Li,Hongliang Jiang,Meijuan Wang,Xiaoqian Wang,Jiangtao Li,Jian Kong,Peng Shi,Hanry Yu,Xingyu Jiang,Qiongyu Guo
Transarterial chemoembolization (TACE), the mainstay treatment of unresectable primary liver cancer that primarily employs nondegradable drug-loaded embolic agents to achieve synergistic vascular embolization and locoregional chemotherapy effects, suffers from an inferior drug burst behavior lacking long-term drug release controllability that severely limits the TACE efficacy. Here we developed gelatin-based drug-eluting microembolics grafted with nanosized poly(acrylic acid) serving as a biodegradable ion-exchange platform that leverages a counterion condensation effect to achieve high-efficiency electrostatic drug loading with electropositive drugs such as doxorubicin (i.e., drug loading capacity >34 mg/mL, encapsulation efficiency >98%, and loading time <10 min) and an enzymatic surface-erosion degradation pattern (∼2 months) to offer sustained locoregional pharmacokinetics with long-lasting deep-tumor retention capability for TACE treatment. The microembolics demonstrated facile microcatheter deliverability in a healthy porcine liver embolization model, superior tumor-killing capacity in a rabbit VX2 liver cancer embolization model, and stabilized extravascular drug penetration depth (>3 mm for 3 months) in a rabbit ear embolization model. Importantly, the microembolics finally exhibited vessel remodeling-induced permanent embolization with minimal inflammation responses after complete degradation. Such a biodegradable ion-exchange drug carrier provides an effective and versatile strategy for enhancing long-term therapeutic responses of various local chemotherapy treatments.
研究领域:
药学
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生物医学工程
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肿瘤学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Doklady Biochemistry and Biophysics
·
2021
·Vol. 497
·期 1
·123-129
·
IF 0.788
Zhao Liang,Luo Yuming,Wang Yijun,Zhao Fujian,Chen Xiaofeng,Cai Daozhang
Abstract This study aimed to evaluate if the 3D printed bioactive glass porous scaffolds (BGS) can improve the reconstruction of the large bone defect. A rabbit model of large bone defects was established by making a 1.0 or 1.5 cm segmental defect in the middle of the femur bone. Then a 1.0 or 1.5 cm BGS was implanted into the bone defect. X-ray imaging showed that in both 1.0 and 1.5 cm groups, the newly formed bone tissue could be observed at 4 weeks after implantation, but a strengthened ossification trend could be observed at different time points. In the 1.0 cm group, a larger number of newly formed bone tissues were observed at 4 weeks, and in the 1.5 group, more newly formed bone tissues were found at 8 weeks. Nevertheless, ossified tissue generation on the BGS mainly completed at 12 weeks after implantation in both groups. The H&E staining revealed that the 3D BGS was easily degraded to form osteoid-like material in vivo , where the neo-ossification gradually occurred from the edge to the center. Immunohistochemical analysis showed that in the 1.0 group, protein expressions of three osteogenesis-related genes- BMP, collagen I and RUNX-2-all peaked at 8 weeks, and then gradually decreased at 12 and 18 weeks. In the 1.5 group, BMP and collagen I peaked at 18 weeks.
研究领域:
骨科
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组织工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2016
·Vol. 6
·期 5
·400-407
·
IF 1.287
Chen Liang,He Shao-Ru,Zhuang Jian,Sun Yun-Xia,Liang Sui-Xin,Liu Yu-Mei,Wang Yi-Fei,Sun Xin,Chen Yan-Ling
Tissue engineering of tracheal with decellularized tracheal matrix has been proposed as a therapeutic alternative to tracheal replacement. At present, the method to acquire decellularized tracheal matrix was not efficient enough, which should be improved. The aim of our study is to
explore a novel method to acquire decellularized tracheal matrix efficiently. Here, the tracheas were taken from New Zealand white rabbits and randomly divided into three groups: Control group (C group), stored in sterile D-Hanks solution; Traditional method group (T group), digested by the
detergentenzymatic method; Novel method group (N group), digested by the Trypsin-EDTA comprised with Detergent-enzymatic method. After the same time of decellularization, the efficacy of decellularization, the histoarchitecture and mechanical properties of decellularized tracheal matrix were
detected in each group. Bone marrow mesenchymal stem cells (MSCs) from neonatal rabbit were seeded in the decellularized tracheal matrix, and SEM was used to detect the growth of MSCs. After the same time of decellularization, most of the cellular components and nuclear material were removed
in N group, compared with C group and T group. SEM and TEM analysis revealed that both N group and T group could retain the hierarchical structure of the native trachea. Manual compression suggested the trachea of T group was much weaker than N group. Tensile test result showed that tensile
breaking load, tensile strength, elongation at break, modulus of elasticity of T group and N group were similar to C group. In addition, 1 days and 3 days after seeded, much more MSCs could adherent in N group than T group. This study suggested that the novel method can generate a decellularized
tracheal matrix superior to the traditional method.
研究领域:
生物医学工程
·
组织工程
·
再生医学
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Chen Lin,Xinyi Bai,Linkun Zhang
Purpose MicroRNA-34c-5p (miR-34c-5p) plays a pivotal role in bone remodeling, yet its therapeutic potential is hindered by challenges such as instability, limited cellular internalization, and immune responses. This study was aimed at developing innovative scaffolds capable of efficiently delivering microRNAs (miRNAs), specifically miR-34c-5p.Methods Chitosan (CS)/sodium tripolyphosphate (STPP)/sodium alginate (SA) scaffolds, referred to as CTS scaffolds, were synthesized at a specific ratio and characterized using dynamic light scattering and scanning electron microscopy (SEM). Cytotoxicity assessments were conducted through cell activity staining. The loading capacity and releasing performance of miRNAs were quantified using spectrophotometry. Subsequently, the in vivo efficacy of miR-34c-5p Agomir/Antagomir in regulating bone repair was evaluated in the rabbit cranial bone defect model, with micro-CT scanning and histological analysis conducted at 4, 8, and 12 weeks.Results CTS scaffolds with a composition ratio of 1:0.2:0.1 were successfully synthesized, exhibiting a mean particle size of 360.1 nm. SEM revealed scaffolds had the porous spongy structure. Cell activity staining confirmed the excellent biocompatibility of the CTS scaffolds. Spectrophotometry demonstrated miR-34c-5p Antagomir were continually released, reaching 91.41% within 30 days. Differential new bone formation was observed between the miR-34c-5p Agomir and Antagomir groups. Micro-CT imaging and histological staining revealed varying degrees of bone regeneration, with notable improvements in the miR-34c-5p Antagomir group.Conclusion CTS scaffolds with a composition ratio of 1:0.2:0.1 demonstrate favorable biocompatibility and enable efficient loading and sustained release of miR-34c-5p Antagomir. The study suggests potential applications of miR-34c-5p Antagomir in promoting bone repair and highlights the promise of innovative scaffolds for therapeutic miRNAs administration in bone regeneration.
研究领域:
生物医学工程
·
组织工程
·
再生医学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 8
·1423-1436
·
IF 4.872
Xinkun Shen,Pingping Ma,Yan Hu,Gaoqiang Xu,Kui Xu,Weizhen Chen,Qichun Ran,Liangliang Dai,Yonglin Yu,Caiyun Mu,Kaiyong Cai
Early mechanical fixation between an implant and native bone is critically important for successful orthopedic implantation, especially for hosts suffering osteoporosis with reduced bone mass. To endow a titanium-based implant with a desirable local anti-osteoporosis property for enhancing its early osseointegration, alendronate-loaded hydroxyapatite-TiO2 nanotube (TNT-HA-Aln) substrates were fabricated and systematically characterized in this study. The results of Aln/Ca2+ release and Ca2+ concentration in an osteoclast medium verified that the release of Aln was significantly accelerated along with the acidity rise caused by osteoclast differentiation. Other in vitro tests, such as CCK-8, alkaline phosphatase (ALP), mineralization, gene expression (Runx2, Osterix, ALP, Col I, OPN, OC, OPG and RANKL), protein production (OPG and RANKL) and tartrate-resistant acid phosphatase (TRAP), proved that TNT-HA-Aln substrates have great potential for improving osteoblast proliferation/differentiation and inhibiting osteoclast differentiation. Moreover, in vivo tests, such as the push-out test, micro-CT and H&E staining proved that TNT-HA-Aln implants could efficiently improve local osseointegration after implantation for 3 months. The study provides an alternative to exploiting drug–device combinations to enhance early osseointegration in osteoporosis.
研究领域:
生物医学工程
·
骨科
·
材料科学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
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