JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 24
·4857-4864
·
IF 2.557
Zhongping Huang,Yueting Xu,Yilei Huang,Charles Liu,Kezhi Jiang,Lili Wang
A novel method based on direct analysis in real time integrated with mass spectrometry was established and applied into rapid determination of ginkgolic acids in Ginkgo biloba kernels and leaves. Instrument parameter settings were optimized to obtain the sensitive and accurate determination of ginkgolic acids. At the sample introduction speed of 0.2 mm/s, high intensity of [M–H] – ions for ginkgolic acids were observed in the negative ion mode by utilization of high-purity helium gas at 450°C. Two microliters of methanol extract of G. biloba kernels or leaves dropped on the surface of Quick-Strip module was analyzed after solvent evaporated to dryness. A series of standard solutions of ginkgolic acid 13:0 in the range of 2–50 mg/L were analyzed with a correlation coefficient r = 0.9981 and relative standard deviation ( n = 5) from 12.5 to 13.7%. The limit of detection was 0.5 mg/L. The results of direct analysis in real time-mass spectrometry were in agreement with those observed by thermochemolysis gas chromatography. The proposed method demonstrated significant potential in the application of the high-throughput screening and rapid analysis for ginkgolic acids in dietary supplements.
研究领域:
分析化学
·
植物化学
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DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2025
·
IF 4.2
Jianbin Shi,Dongfang Fang,Yong Sui,Tian Xiong,Xueling Chen,Chuanhui Fan,Deshun Zhou,Fang Cai,Xin Mei
Sweet potato ( Ipomoea batatas L.) leaves are rich in polyphenols and consumed as leaf vegetable in world. The content and composition of polyphenols are diversity at different harvesting period. This study investigated the changes of total polyphenols content (TPC), antioxidant capacity, and polyphenol fractions content of sixteen varieties of SPLs during growth stage. The results showed significant diversity in the concentrations of active compounds in different genotypes and growth stages. TPC ranged from 27.45 to 243.82 mg CAE/g DW among the varieties over various growth stages, with G3 and E1 varieties exhibiting relatively high TPC and antioxidant capacity. Twelve polyphenol fractions were identified in SPLs. Correlation analysis demonstrated a significant positive relationship between antioxidant capacity and the content of monosubstituted caffeoylquinic acids (3-caffeoylquinic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid) and disubstituted caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid). Principal component analysis highlighted the superior performance of the E1 variety in terms of polyphenol content and antioxidant capacity.
研究领域:
植物学
·
食品科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Xueying Song,Jiamu Kang,Xingyan Wei,Liu Liu,Yongfeng Liu,Feng Wang
Shigella flexneri has the ability to contaminate pork and cause foodborne diseases. This study aimed to examine the effectiveness of linalool (a natural preservative) against S. flexneri and explore its potential application in contaminated pork. The results showed that linalool was capable of damaging the cell membrane and binding to the DNA of S. flexneri , and inhibiting biofilm formation and disrupting mature biofilms. The antibacterial effectiveness of linalool on the surface of pork was further demonstrated by analyzing the physicochemical properties of the pork ( i.e. , weight loss rate, pH value, color index, and TVB-N value) and its protein profiles. Linalool did not completely kill S. flexneri in pork at minimum bactericidal concentration (MBC) concentration and its antibacterial effect of linalool was stronger during the initial stage of storage. During storage, linalool influenced the abundance of specific proteins in the pork, particularly those involved in pathways related to fat metabolism. These findings offer novel insights into the antibacterial efficacy of linalool and its underlying mechanism in pork.
研究领域:
食品科学
·
微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 20
·3295
·
IF 4.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Loquat leaf extract (LLE) was added to guar gum and pullulan as an environmentally friendly packaging film (GPE) to preserve Chinese water chestnuts (CWCs). The effect of the amount of LLE on the guar gum/pullulan composite film was investigated. The optimal amount of LLE was 4% (GPE4), with lower water vapor permeability (WVP) and greater mechanical strength, antioxidant, and comparable antibacterial performance than many pullulan-based films. Upon packing the CWCs for 4 days, the weight loss rate of GPE4 was only 1.80 ± 0.05%. For GPE4, the POD activity, the soluble solid content, and the vitamin C (Vc) content of the CWCs were 21.61%, 36.16%, and 26.22% higher than those of the control sample, respectively. More importantly, GPE4 was effective in preserving the quality of CWCs after 4 days of storage, better or at least comparable to non-biodegradable plastic wrapping (PE). Therefore, it can be concluded that GPE films hold significant promise as a sustainable alternative packaging material for preserving fruit-based foods like CWCs, potentially replacing PE in the future.
研究领域:
食品科学
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包装工程
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Zhihong Huang,Lu Wang,Xinjie Li,Bingjun Lu,Yuli Lin,Bo Fu,Chiqun Shan,Xinxin Tang,Pei Sung Toh,Shaoyou Lu
With the widespread use of plastic products, phthalates (PAEs), as common plasticizers, have become a significant contributor to environmental pollution. PAEs can migrate to tea leaf during cultivation, processing, and packaging, and can be released into the tea infusion during brewing, posing potential risks to human health. A total of 220 tea samples from four major tea-producing regions in China were collected and analyzed for the concentrations of 13 PAEs in tea leaves and tea infusions. PAEs were present in all tea samples at concentrations ranging from 504.4 to 6177.0 ng/g, with dibutyl phthalate (DBP) contributing the most. Dark tea and tea from the eastern coast and southern regions of China were the most contaminated. The brewing experiments indicated that PAEs could migrate into tea infusions, with concentrations ranging from 0 to 77.4 ng/mL, showing the highest transfer rates during the second and third brews. The health risk assessment results revealed that PAEs posed low noncarcinogenic and carcinogenic risks through tea consumption, but risk levels were significantly higher in children than in adults. This indicates the necessity for giving children more attention. We suggest stricter environmental protection measures during tea cultivation, processing, and packaging to safeguard public health.
研究领域:
环境科学
·
食品科学
·
公共卫生
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Nan Li,Feng-xiang Pang,Yu-wan Zhou,Shou-chang Ding,Chuan-jian Shi,Rui-jia Wen,Yong-xin Mai,Xian-lin Wu,Jin-fang Zhang
Background Osteosarcoma (OS) is a malignant primary tumor in bone tissues, and Methotrexate (MTX) is widely used as a chemotherapeutic drug for OS in clinical treatment. However, MTX resistance is a major challenge for OS patients. Polydatin (PD) is an extract derived from Reynoutria japonica Houtt and has been identified as a potential agent for reversing chemotherapy resistance in cancer. We therefore wondered whether PD could alleviate MTX resistance in OS. Methods The effect of PD on MTX resistance was investigated. And H19 expression was examined in the PD-treated OS cells. The association between H19 and MTX resistance in osteosarcoma was investigated.The effects of H19 on MTX resistance in osteosarcoma were evaluated by cell proliferation, colony formation, ChIP and western blotting assays. In order to investigate the molecular mechanism of PD underlying methotrexate resistance in OS, the cell proliferation, colony formation, q-PCR and western blotting were examined. Results In the present study, PD was found to act as a novel MTX sensitizer, and significantly enhanced the suppressive effects of MTX on OS in vitro and in vivo . Mechanically, PD was demonstrated to suppress the expression of long non-coding RNA H19 as well as its binding protein histone methyltransferase Enhancer of zeste homolog 2 (EZH2), and thus reduced the trimethylation of histone H3K9 (H3K9me3) and K27 (H3K27me3). Furthermore, H19 knowndown increased the binding ability of H3K9me3 and H3K27me3 with the folate metabolism-related genes RFC-1 and PCFT's promoter, and influenced folate metabolism and MTX resistance. Conclusion In conclusion, our results indicated that PD alleviated MTX resistance in OS via the H19/H3K27me3/H3K9me3 mediated folate metabolism regulatory axis. The translational potential of this article The findings of this study validate the function of PD in alleviating MTX resistance, and provide the new insight into developing PD as a promising drug candidate for the MTX resistant OS patients.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Jingjing Du,Tianying Tao,Mengxi Gao,Xueting Zhang,Xilin Wang,Qian Zhang,Yuanqian Xu,Baodan Jin,Lan Wang,Xia Cao
Nanoplastics pose a potential threat to a wide variety of aquatic organisms . Despite the awareness of this existing hazard, the impact of nanoplastics on natural fungal communities remains a research gap. In this study, five dominant fungi species, isolated from a stream ecosystem, were used to explore the effects of different nano-polystyrene (nano-PS) particles concentrations on a simulated fungal community. Specifically, the evaluation was conducted regarding the fungal growth , reproductivity, structural composition, and ecological function in leaf litter decomposition. A 15-day exposure experiment showed that 100 μg/L nano-PS significantly reduced the microcosm pH. The extracellular enzyme activities of β-glucosidase, leucine-aminopeptidase, and peroxidase were significantly promoted by nano-PS exposure for 5 days or 15 days. Total sporulation rate significantly decreased after the 15-day exposure to 1 and 100 μg/L nano-PS and significantly increased under 10 μg/L nano-PS. In contrast, nano-PS concentrations had no effects on fungal biomass . In addition, the reduced relative abundance of Geotrichum candidum lowered its contribution to leaf decomposition, resulting in a decreased litter decomposition rate of a 24.5–27.9 % after exposure. This suggests that 1–100 μg/L nano-PS inhibited leaf decomposition by inhibiting fungal reproduction and reducing the contribution of specific fungal species. In addition, the findings highlight the importance of exploring the potential mechanisms of the interaction between nanoplastics and fungal species.
研究领域:
生态学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Therapeutic Delivery
·
2025
·
IF 2.2
Lixin Fan,Yang Yu,Kunpeng Shi,Yanqiu Hu,Xingyu Hou,Weitong Sun
Objective The presence of overexpressed folate-receptor and high concentration of glutathione (GSH) in liver cancer cells has been exploited and we have synthesized Folic acid-Dextran-Cystamine-Stearic acid (FDCS) for efficient and multifunctional delivery of the drug sorafenib (SAF) to enhance the anticancer effects.Methods The characteristics of micelles such as physicochemical properties and in vitro release were investigated. The pharmacokinetic characteristics of the micelle and SAF groups in rats were investigated. In vitro and in vivo anti-tumor experiments were performed with HepG2 cells.Results SAF-FDCS was successfully prepared and characterized. The cellular experiments showed that SAF-FDCS significantly enhanced the toxicity and inhibitory effects on HepG2 cells compared with free drug and the other micelles without smart response, and the uptake capacity of cellular HepG2 for SAF-FDCS was significantly higher than the groups without folic acid. Pharmacokinetic results showed that SAF-FDCS revealed a longer circulation time than free SAF. In addition, the tumor inhibition rate of SAF-FDCS in the subcutaneous graft tumor model of HepG2 cells was 84.6%, significantly higher than in other groups.Conclusions These results demonstrated the feasibility of SAF-FDCS micelles in inhibiting tumor growth and their superiority in anti-cancer effects compared to free drugs and normal micelles.
研究领域:
药学
·
肿瘤学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 18
·14072-14078
·
IF 4.6
Beixiao Zhang,Gul Muhammad,Liya Deng,Md Asraful Alam,Anqi Zhao,Thomas O. Butler,Zhenglong Li,Ximing Zhang,Jingliang Xu
Microalgae biomass is regarded as a potential feedstock for valuable compounds such as pigments, lipids and proteins. However, development of single molecule extraction processes is the most common practice. A green multiproduct extraction approach is needed for economically sustainable process development of the microalgal industry. Therefore, this study aims to investigate the sequential extraction of lutein and lipid from dry and wet Chlorella pyrenoidosa biomass pretreated with a choline chloride-based deep eutectic solvent (DES) under a sustainable biorefinery scheme. In this context, we have assessed the kinetic modeling of the solid–liquid extraction process for the aforementioned compounds, focusing on the effects of temperature and time. The maximum lutein (3.80 mg g−1) and lipid (95.0 mg g−1) contents from dry biomass were obtained at 45 °C in 40 min and at 70 °C in 90 min, respectively. From wet biomass, the maximum lutein (2.57 mg g−1) and lipid contents (87.47 mg g−1) were obtained at 35 °C in 40 min and at 70 °C in 90 min, respectively. The kinetics of the solvent-based extraction process for lutein and lipids were assessed via first-order and second-order kinetic models with an associated investigation of kinetic parameters, such as rate constants, saturation concentration and activation energies. We found that temperature is an important parameter that influences the extraction of all compounds and also has a significant impact on the kinetic parameters. Toxicity evaluation of the DES and economic assessment of DES vs. ionic liquids (ILs) were performed. The synthesis cost of the DES is lower than that of ILs, and Escherichia coli JM109 survivability assessment confirms the DES as a non-toxic solvent. The present study provides valuable insights into the sequential extraction for a high-value multiproduct biorefinery.
研究领域:
生物技术
·
可再生资源与环境
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Meiling Wang,Guowen Meng,Qing Huang
A prototype of environmentally friendly and low-cost fluorescence sensor, consisting of spinach-extracted chlorophyll- a modified alkali-treated peanut shell (denoted as chlorophyll- a @peanut shell), is achieved and used for trace detection of Hg 2+ in aqueous solutions through fluorescence quenching method. The chlorophyll- a @peanut shell exhibits a linear dynamic detection range from 0 to 19 × 10 −8 M for Hg 2+ with a detection lower limit of 8.5 × 10 −9 M. The feasibility of sensitive and selective detection of Hg 2+ using the chlorophyll- a @peanut shell is also demonstrated in the test of Hg 2+ in synthetic polluted lake waters, showing excellent anti-interference ability to common impurities like silt and alga. Further experiments demonstrate that the improved sensing property of the chlorophyll- a @peanut shell for Hg 2+ than that of chlorophyll- a in solution is ascribed to the selective Hg 2+ adsorption of the alkali-treated peanut shell, which might also be used for the remediation of Hg 2+ in polluted waters. The design concept using non-toxic plants may open a new door to the environmentally friendly and low-cost materials for detection and remediation of environmental pollutants .
研究领域:
环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 44
·期 4
·1500-1507
·
IF 3.069
Yaoyao Qu,Liying Yu,Baoya Zhu,Fang Chai,Zhongmin Su
Herein, natural celery leaves and glutathione were used as the precursors to prepare carbon dots (CDs) as novel fluorescent paper sensors, which exhibited high sensitivity and selectivity for the detection of explosive nitrophenols (including 2-nitrophenol (2-NP), 3-nitrophenol (3-NP) and 4-nitrophenol (4-NP)). The prepared CDs featured blue fluorescence emission under irradiation using an UV lamp, and the fluorescence quantum yield was as high as 53%. As fluorescent probes, the CDs showed a particular response to 2-NP, 3-NP and 4-NP, and the detection limits of 2-NP, 3-NP and 4-NP were determined to be 39 nM, 43 nM and 26 nM, respectively. Due to the multiple advantages including good water solubility, low toxicity and excellent stability, CDs are expected to be widely used. Meanwhile, a CD-based test paper was exploited and it displayed a distinctive quenching response when exposed to 3-NP and 4-NP, with the visual detection limits of 0.5 μM and 0.1 μM, respectively. Furthermore, the low toxicity and biocompatibility of CDs were also demonstrated by evaluating cell culture experiments, and CDs showed good potential applications in imaging. In summary, the prepared CDs are promising for fluorescent paper sensors and bioimaging.
研究领域:
化学传感
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环境科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
PHYTOPATHOLOGY
·
2023
·
IF 3.2
Mr. Jiayu Yin,Ms. Shuang Wu,Ms. Yongli Yang,Dr. Delu Wang,Prof. Yue Ma,Dr. Yongtian Zhao,Prof. Sujitraj Sheth,Miss Honglin Huang,Dr. Baoan Song,Dr. Zhuo Chen
Carvacrol expresses a wide range of biological activities, but the studies of its mechanisms focused on bacteria, mainly involving the destruction of the plasma membrane. In this study, carvacrol exhibited strong antifungal activities against several phytopathogenic fungi and determined a novel antifungal mechanism against Lasiodiplodia theobromae. RNA sequencing indicated that many genes of L. theobromae hyphae were predominately induced by carvacrol, particularly those involved in replication and transcription. Hyperchromic, hypsochromic, and bathochromic effects in the UV-visible absorption spectrum were observed following titration of calf thymus DNA (ctDNA) and carvacrol, which indicated the formation of a DNA–carvacrol complex. Circular dichroism (CD) spectroscopy indicated the response of DNA to carvacrol was similar to 4’, 6-diamidino-2-phenylindole (DAPI), but different from that of ethidium bromide (EB), implying the ionic bonds between carvacrol and ctDNA. Fluorescence spectrum (FS) analysis indicated that carvacrol quenched the fluorescence of double-stranded DNA more than single-stranded DNA, indicating that carvacrol mainly bound to double-stranded DNA. A displacement assay showed that carvacrol reduced the fluorescence intensity of the DNA–DAPI complex through competing with DAPI, but this did not occur for DNA–EB. FS assay revealed that carvacrol bound to AAA sequence on the minor groove of ds-oligonucleotides. The hydroxyl of carvacrol was verified to bind to ctDNA through a comparative test in which structural analogs of carvacrol, including thymol, 4-ethyl-1,2-dimethyl, etc. were analyzed. The current study indicated carvacrol can destruct plasma membranes and bind to the minor groove of DNA, which inhibited the fungal proliferation by disturbing the stability of dsDNA.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）