JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 43
·期 3
·e12741
·
IF 1.552
Si-Qi Yang,Xiao-Yan Dai,Xiao-Yi Wei,Qing Zhu,Tao Zhou
Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N , N′ -dicyclohexylcarbodiimide/ N -hydroxysuccinimide as activating agent. The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively. Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts. Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse. Conditions for the hydrolysis of the pumpkin–hawthorn compound juice by I-PG were optimized using orthogonal experiments. After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably. Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin–hawthorn juice. Practical applications In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed. The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Hao Huang,Tarun Belwal,Halah Aalim,Li Li,Xingyu Lin,Songbai Liu,Caixia Ma,Qunhe Li,Ying Zou,Zisheng Luo
This study aimed to examine the modification effect of whey protein concentrate (WPC), WPC-gum arabic (WPC-GA) or WPC-high methoxyl pectin (WPC-PEC) complex to tailor-modify W/O/W emulsion for secondary microencapsulation of hydrophilic arbutin and hydrophobic coumaric acid. The stability and rheological properties of coated emulsions, encapsulation yield, release and degradation kinetics of arbutin and coumaric acid were investigated. Results revealed that WPC-PEC complex (at the ratio of 1:3) coating W/O/W emulsion exhibited the highest viscosity and stability, with the highest encapsulation yield of 91.08% for arbutin and 80.92% for coumaric acid, respectively. Tighter coating structure of the WPC-PEC complex (1:3) forming a stronger gel network structure was confirmed, accounting for the larger mean particle size of 569.67 nm. Moreover, the WPC-PEC (1:3) coating W/O/W emulsion also showed controlled release of arbutin and coumaric acid in simulated conditions. The k value of degradation kinetics for arbutin (7.99 × 10 −4 at pH = 1.2, 4.19 × 10 −4 at 90 °C and 7.52 × 10 −4 at UV-C treatment) and coumaric acid (5.18 × 10 −4 at pH = 1.2, 3.24 × 10 −4 at 90 °C and 6.90 × 10 −4 at UV-C treatment) indicated low degradation rate. The present study revealed that the WPC-PEC (1:3) coating W/O/W emulsion could provide a better synergistic effect on higher encapsulation yield and stability of arbutin and coumaric acid.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Kun Feng,Chen Li,Yun-Shan Wei,Min-Hua Zong,Hong Wu,Shuang-Yan Han
Advances in pharmaceutical technology have promoted the development of colon-targeted delivery system for oral administration of bioactive peptides or proteins to enhance their bioavailability. In this study, a multi-unit nanofiber mat was fabricated by coaxial electrospinning and its feasibility as the colon-targeted delivery system for a bioactive peptide, salmon calcitonin (sCT), was investigated. Sodium alginate and sCT-loaded liposome coated with pectin served as the shell layer and core layer, respectively. An in vitro study demonstrated that the encapsulated sCT was released in a sustained and colon-targeted way. Analysis using different mathematical models showed that release followed a complex mechanism. In addition, greater amounts of sCT were released from the core-shell nanofiber mat into simulated colon fluid (SCF) than was released from a uniaxial nanofiber mat (65.2% vs. 47.8%). The use of a core-shell nanofiber mat further alleviated the burst release of sCT into simulated gastric and intestinal fluid (SGF and SIF), demonstrating the superiority of a multi-unit vehicle for colon-targeted delivery of sCT. Furthermore, 88% of the bioactivity of encapsulated sCT was retained. This multi-unit vehicle offers a better-designed vehicle for the colon-targeted sustained release of bioactive peptides or proteins and, thus, should improve oral bioavailability.
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JOURNAL OF INORGANIC BIOCHEMISTRY
·
2018
·
IF 3.063
Jiajing Diao,Feng Bai,Ying Wang,Qianqian Han,Xi Xu,Hongmei Zhang,Qiang Luo,Yanqing Wang
In this work, we designed polysaccharide-metal complex of dopamine-modified pectin linking ruthenium compound, which exhibited a certain inhibition specificity to human renal cell adenocarcinoma cell line 786-O. The chemical structure and physical properties of the polysaccharide-metal complex were well characterized by multiple analysis methods. The multi-spectral results revealed that pectin-dopamine have been successfully coordinated by ruthenium complex to form nano-conjugates, which self-assembled into relatively regular nanospheres of approximately 200 nm. The polysaccharide-metal complex has more amorphous and less viscosity than pectin, and cannot withstand as much strain as the pectin and pectin-dopamine systems. Pectin-dopamine can decrease the toxicity effect of Ru complex in normal cell line such as human normal renal epithelial cell line 293A.
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Hong-Bing Chen,Xin-Lei Li,Ming-Jun Chen,Yan-Rong He,Hai-Bo Zhao
Self-cross-linked aerogel based on pectin and melamine-formaldehyde resin (MF) was fabricated via freeze-drying method using water as solvent, where pectin is structural material meanwhile acting as acid to catalyse the cross-linking of MF. The cross-linking reaction easily occurs without additional additives, which can be significantly accelerated at elevated temperatures, with a critical value of about 55 °C. The obtained aerogel shows network microstructures as observed with SEM. With increasing pectin content, the aerogel shows significantly increased compressive modulus. The compressive modulus of M10Pe5 arrives 23.2 MPa, the specific modulus of which arrives 188 MPa cm 3 /g, while pure MF aerogel are too fragile to keep intact after freeze-dried. The resulting aerogel has good thermal stability, excellent water resistance (can be second dried with limited strength loss) and low flammability. This partially bio-based novel aerogel with impressive properties is promising in many applications.
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ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Yang Fan,Pei-Fang Liu,Zi-Juan Yang,Tong-Wu Jiang,Kai-Li Yao,Ran Han,Xin-Xin Huo,Yun-Yi Xiong
Porous carbon spheres (PCSs) are prepared via a facile hydrothermal carbonization and chemical activation route with pectin as the biomass precursor. The as-obtained PCSs present a large specific surface area (2440 m 2 g −1 ), well-balanced micro/mesoporosity with narrow pore size distribution and high content of oxygen-containing functional groups. The structure features make the PCSs an ideal electrode material for electrochemical energy storage . As electrode material for supercapacitors , the PCSs exhibit a high specific capacitance, good rate capacity and cycling stability. Furthermore, the PCSs show attractive property as support material for Pt electrocatalysts . Pt nanowires (PtNWs) grown on the PCSs interconnect with each other to form a porous nanowires network structure. The three-dimensional (3D) hybrid catalyst material consisting of PtNWs network supported on PCSs exhibit superior catalytic activity and stability towards both methanol and ethanol electro-oxidation in acidic media. In addition, owing to the rich presence of surface oxygen groups on PCSs, the CO-poisoning tolerance of the supported Pt nanowires is greatly promoted.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Liufeng Lin,Wei Xu,Hongshan Liang,Lei He,Shilin Liu,Yan Li,Bin Li,Yijie Chen
Novel nano-particles were developed from lysozyme-pectin through self-assembly, and the nanogels could be used as a carrier for the antitumor agent, methotrexate (MTX). The nanogels exhibited spherical with diameters about 109 ± 2 nm and narrow particle size distribution, as well as negative surface charge. Furthermore, the particle size and morphology of the nanogels hardly changed with the incorporation of MTX. The loading capacity of MTX in nanogels could reach 17.58 ± 0.85%. MTX-loaded nanogels were pH-dependent, accelerated release of MTX at a decreasing pH from 7.4 to 5.3. The MTT assay indicated that encapsulated MTX exhibited higher anticancer activity than free MTX. Meanwhile, MTX-loaded nanogels could be effectively endocytosed by HepG2 cells, resulting in enhanced cancer-cell apoptosis comparing to free MTX. It indicated that the nanogels had good biocompatibility and low toxicity. The obtained nanogels had great potential in the development of a new nanocarrier for anti-cancer drug delivery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiahao Ren,Yifang Cao,Yan Zhang,Xinglong Xiao,Qi Zhang,Qiyue Yang,Fengsong Liu
In this study, (3-Aminopropyl) triethoxysilane (APTES) was employed as a bridging agent to enhance the compatibility between the hydrophilic starch/pectin film and the hydrophobic palmitic acid (PA) coating through hydrogen bonding and chemical reactions. To address the insufficient antibacterial activity of starch films, geraniol was also incorporated. The intermolecular interactions among APTES, PA, and starch were confirmed using x-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and hydrogen nuclear magnetic resonance spectroscopy ( 1 H NMR). Notably, the inclusion of APTES and PA significantly increased the film's hydrophobicity, resulting in a water contact angle (WCA) of 95.12°, a water vapor permeability (WVP) of 2.08 × 10 −10 g/(mm·s·Pa), and an oxygen permeability (OP) of 2.61 × 10 −9 g·mm·mm −2 ·s −1 . Molecular dynamics (MD) simulations revealed strong non-covalent interactions and exceptional compatibility between starch and PA. Furthermore, the integration of pectin and geraniol improved the mechanical strength and antimicrobial properties of the modified films compared to unmodified starch films. These environmentally friendly and biodegradable starch-based films present a promising option for sustainable packaging materials in food preservation.
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RSC Advances
·
2024
·Vol. 14
·期 11
·7572-7581
·
IF 3.9
Shanshan Tie,Qing Zhang,Yixuan Zhao,Ying Wu,Dasu Liu,Lina Zhao,Shaobin Gu
The purpose of this study was to design a novel antioxidant and antibacterial film for food packaging using food-grade raw materials. The films were designed and fabricated based on carboxymethyl chitosan and pectin incorporated with procyanidins (PCs) and phycocyanin (Phy) by the tape casting method. The effects of different proportions of PCs and Phy on the properties and functions of the prepared films were studied. The results showed that the thickness of films could range from 55 to 70 μm, with dense network structure and uniform distribution of elements. Compared with C-Film group, the film loaded with PCs and Phy had lower water solubility and swelling rate, and higher tensile strength and elongation at break. FITR and XRD spectra revealed the molecular interaction mechanism among carboxymethyl chitosan, pectin, PCs and Phy, which could effectively endow the films with ultraviolet barrier properties. Moreover, the addition of PCs and Phy could effectively improve the antioxidant capacity and antibacterial effect of films, for example, the free radical scavenging abilities of most films were above 80% when the concentration of PCs was 40 μg mL−1. In view of these functional properties, the prepared film containing PCs and Phy have been successfully used in food packaging, which was proved by the preservation experiment of grapes. This study can provide theoretical and technical guidance for the preparation of biodegradable antibacterial films, and their application in the food packaging field.
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Food Bioscience
·
2024
·
IF 4.8
Jinman He,Siyuan Yang,Gulden Goksen,Xinli Cong,Mohammad Rizwan Khan,Wanli Zhang
It was focused on development a film with excellent properties. The pectin/sodium alginate (PS) composite films were developed by a casting technology, incorporating with tannic acid and Fe 3+ for double cross-linking, and the effects of the film thickness, optical, mechanical, barrier, and antioxidant properties were investigated. This results displayed the inclusion of tannic acid (TA) and Fe 3+ considerably increased the physical aspects, and they were very compatible with the matrix of the PS, exhibiting higher UV barrier capacity and lower gas permeability . On the other hand, the more compact structure observed by SEM as well as the hydrogen bonding interactions between the film components revealed by FTIR spectroscopy and XRD diffraction analyses further confirmed the significant enhancement of the properties. The functionalized coating of TA-Fe 3+ demonstrated an improved adhesion and barrier properties, thereby effectively retarding the ageing process of golden passion fruit and this encompassed a decrease in the weight loss and a significant enhancement in water retention capability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Liting Zhang,Mingjun Qiao,Haoyu Zheng,Tony Vancov,Vasileios Antoniadis,Sabry M. Shaheen,Stephen Joseph,Chao Chen,Shengdao Shan,Hanbo Chen,Hailong Wang
Herein, a series of novel carboxymethyl cellulose (CMC)/pectin-based hydrogel beads were synthesized with chitosan as a cross-linker. The hydrogel composites were denoted as CPC-1/2/3/4/5, with varying mass ratios of CMC, pectin, and chitosan (6:0:1, 5:1:1, 4:2:1, 3:3:1, and 2:4:1). This is a pioneering study that investigates the synergistic effects of these biopolymers in a single hydrogel system for the adsorption of heavy metals, specifically Cd 2+ , Pb 2+ , and Cu 2+ . CPC-4 was identified as the optimal adsorbent, and its adsorption behavior, efficiency, and mechanisms were systematically explored. The adsorption kinetics of CPC-4 for Cd 2+ , Pb 2+ , and Cu 2+ adhered to the pseudo-second-order model, while equilibrium adsorption isotherms were best described by the Langmuir model. Notably, CPC-4 achieved maximum adsorption capacities for Cd 2+ , Pb 2+ , and Cu 2+ of 139.4, 270.3, and 143.6 mg/g, respectively. Spectroscopic analyses including FTIR and XPS revealed abundant oxygen- and nitrogen-bearing functional groups (carboxyl, hydroxyl, and amine) in CPC-4, which facilitated metal ion binding through complexation reactions. Density functional theory calculations demonstrated that the incorporation of chitosan significantly enhanced the interaction between CMC and pectin, identifying the coupled regions as the most favorable sites for metal adsorption, with adsorption energies of −4.03 eV for Cd, −4.59 eV for Pb, and −5.59 eV for Cu. The superior adsorption performance of CPC-4 is primarily attributed to complexation and hydrogen bonding at the cross-linked sites. In summary, this study highlights the potential of this biopolymer-based hydrogel composite as a promising new adsorbent for the effective management of heavy metal contamination in aquatic environments.
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SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Xiaojie Pei,Shuxin Sun,Xin Dai,Ke Liu,Ying Yang,Laifeng Lu,Yansheng Wang
Goji berry ( Lycium barbarum ) is a typical berry with a tender and thin pericarp, high moisture content and hollow interior, making it highly susceptible to mechanical damage caused by harvest and transportation. As a consequence, this susceptibility results in considerable economic losses within the fresh produce industry due to the degradation of fruit quality and subsequent decay. Here, oligogalacturonides (OGs), a well-characterized plant damage-associated molecular pattern, were applied to reduce postharvest loss and extend the shelf life of berries of the goji cultivar NingQi1. The loss rate in the OGs treatment was 44.45 % lower than that in the 0.8 mm compression treatments on Day 6, and the OGs treatment also effectively inhibited softening and delayed weight loss. The activity of superoxide dismutase increased by 27.4 % on the 4th day after OGs treatment, accompanied by a decrease in malondialdehyde content ranging from 14.2 % to 24.8 % on days 2 to 4 compared to the control group. Furthermore, the phenylalanine ammonia-lyase activity of OGs-treated goji berries was 57.0 % higher than that of the control at 6th days, which slowed the reduction in secondary metabolites such as total flavonoids. The findings of this study indicated that OGs may reduce postharvest losses caused by mechanical damage, extend the shelf life of goji berry by reducing softening and weight loss, and reduce wounding injury by minimizing free radical form of reactive oxygen species.
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