PEST MANAGEMENT SCIENCE
·
2022
·Vol. 78
·期 6
·2240-2249
·
IF 4.462
Tan Dai,Zhiwen Wang,Xingkai Cheng,Huige Gao,Li Liang,Pengfei Liu,Xili Liu
BACKGROUND Fungicide resistance has become a serious problem for different mode of action groups except for uncouplers, which makes their resistance mechanism a hot topic, which until now, has not been well clarified. SYP-14288, a newly developed diarylamine fungicide modeled on fluazinam, has shown good toxicity to both oomycete and fungus by the action of uncoupling. In this research, the resistance of Phytophthora capsici to SYP-14288 was studied to clarify the resistance mechanism of uncouplers. RESULTS The toxicity tests of resistant strains against SYP-14288 showed multidrug resistance. The high-performance liquid chromatography (HPLC) results showed that resistant strains could efflux the fungicide, and this ability could be inhibited by the efflux pump inhibitor amitriptyline. The target protein of amitriptyline is P-glycoprotein (P-gp), which was overexpressed in resistant strains. Three products of nitrate reduction of SYP-14288 were detected and determined by HPLC-Q-TOF. Eight cytochrome P450 monooxygenase (P450) proteins were differentially involved in the reduction reaction. CONCLUSION Both fungicide efflux and detoxification metabolism were involved in the resistance mechanisms of P. capsici to SYP-14288. © 2022 Society of Chemical Industry.
研究领域:
植物病理学
·
分子生物学
·
杀菌剂科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2025
·
IF 2.1
Yufan Yang,Muran He,Yichan Zhang,Chunting Zhou,Chunhuan Luo
The cis-1-chloro-3,3,3-trifluoropropene (R1233zd(Z)) and tetraethylene glycol dimethyl ether (TEGDME) system has been proposed in this study as a potential alternative absorption working pair. The vapor pressure, density, viscosity, and specific heat capacity of the R1233zd(Z)/TEGDME system were measured systematically. The mixing enthalpy and specific enthalpy were calculated by the NRTL model. Regression equations for the measured values were obtained by a least-squares method. Compared with the LiBr/H2O system, the generation temperature of the R1233zd(Z)/TEGDME system is reduced by 12 °C and the viscosity by about 50%. R1233zd(Z)/TEGDME has strong practical application significance in the absorption heat pump system.
研究领域:
化学工程
·
热力学
·
制冷技术
✓ 已验证
产品关联
DOI
高影响因子
eLife
·
2025
·
IF 0
Yajun Zhai,Peiyi Liu,Xueqin Hu,Changjian Fan,Xiaodie Cui,Qibiao He,Dandan He,Xiaoyuan Ma,Gongzheng Hu
Discovering new strategies to combat the multidrug-resistant bacteria constitutes a major medical challenge of our time. Previously, artesunate (AS) has been reported to exert antibacterial enhancement activity in combination with β-lactam antibiotics via inhibition of the efflux pump AcrB. However, combination of AS and colistin (COL) revealed a weak synergistic effect against a limited number of strains, and few studies have further explored its possible mechanism of synergistic action. In this article, we found that AS and EDTA could strikingly enhance the antibacterial effects of COL against mcr-1- and mcr-1+ Salmonella strains either in vitro or in vivo, when used in triple combination. The excellent bacteriostatic effect was primarily related to the increased cell membrane damage, accumulation of toxic compounds and inhibition of MCR-1. The potential binding sites of AS to MCR-1 (THR283, SER284, and TYR287) were critical for its inhibition of MCR-1 activity. Additionally, we also demonstrated that the CheA of chemosensory system and virulence-related protein SpvD were critical for the bacteriostatic synergistic effects of the triple combination. Selectively targeting CheA, SpvD, or MCR using the natural compound AS could be further investigated as an attractive strategy for the treatment of Salmonella infection. Collectively, our work opens new avenues toward the potentiation of COL and reveals an alternative drug combination strategy to overcome COL-resistant bacterial infections.
研究领域:
微生物学
·
药理学
·
传染病学
·
耐药性
✓ 已验证
产品关联
DOI
PubMed
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Qiuru Chen,Yulei Liang,Yanbin Dong,Junling Cui,Kun He,Xiaoyuan Ma,Jinfeng Zhao,Yajun Zhai,Li Yuan
H-NS is a histone-like nucleoid-structuring protein that regulates gene expressions, particularly acquired foreign genes, however, little is known about whether H-NS can modulate bacterial susceptibility by regulating its intrinsic genes. The hns-deleted mutant EΔhns, the hns-complemented strain EΔhns/phns and the hns-overexpressed strain E/phns were derivatives of Escherichia coli ATCC 25922, the susceptibility of which were assessed by the broth microdilution method and time-kill curves assays. We found that the MICs for strain EΔhns against gentamicin and amikacin were significantly decreased by 8–16 folds in contrast to E. coli ATCC 25922. Further studies displayed that the absence of hns caused damage to the bacterial outer membrane and increased the expression levels of porin-related genes, such as ompC, ompF, ompG, and ompN, thus obviously enhancing aminoglycosides uptake of strain EΔhns. Meanwhile, hns deletion also led to remarkable inhibition of the efflux pumps activity and decreased expressions of efflux-related genes clbM, acrA, acrB, acrD, and emrE, which reduced the efflux of aminoglycosides. In addition, the activation of glycolysis and electron transport chain, as well as the reduction of Δψ dissipation, could lead to a remarkable increase in proton motive force (PMF), thus further inducing more aminoglycosides uptake by strain EΔhns. Our findings reveal that H-NS regulates the resistance of E. coli to aminoglycosides by influencing its uptake and efflux, which will enrich our understanding of the mechanism by which H-NS modulates bacterial resistance.
研究领域:
微生物学
·
分子生物学
·
抗菌素耐药性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Quan Zou,Hu Li,Yuping Yang,Yingchun Miao,Yuning Huo
A novel photocatalytic reactor was designed for the photo-degradation of organic pollutants with floated glass balls on the solution surface, which were constantly rotated by the air pumping and coated with Bi 2 O 3 /TiO 2 film. The uniform and stable Bi 2 O 3 /TiO 2 film was prepared by the facile sol-gel method at the optimal conditions of Bi Bi 2 O 3 content (1.0 mol%) and the three coating times with the film thickness of 210 nm. It was uniformly distributed and stably combined with the surface of the glass ball. Modification of Bi 2 O 3 significantly improved the visible light absorption of TiO 2 film and inhibited the photo-induced charges, promoting the photo-degradation activity. More importantly, the air pumping at the bottom of the reactor impelled the constant rotation of floated glass balls. It could realize the uniform aqueous membrane on the catalyst film to improve the efficient contact between catalyst and organics and to utilize the irradiation light efficiently, inhibiting the light shield effect of dyeing wastewater and facilitating the photo-degradation. Moreover, the Bi 2 O 3 /TiO 2 photocatalytic film in this photocatalytic reactor exhibited high durability and prevented the leakage of catalyst, providing a potential for the practical applications in the future.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
Energy & Environmental Science
·
2024
·
IF 32.4
Xin Jin,Mengfan Pei,Dongming Liu,Zihui Song,Wanyuan Jiang,Runyue Mao,Borui Li,Xigao Jian,Fangyuan Hu
Sluggish kinetics limit the practical application of sodium-ion batteries (SIBs); thus, innovative strategies and the design of materials with fast reaction kinetics are important for the development of SIBs. To solve these issues, the innovative strategy of using a micro-stress pump to boost Na+ transport by simulating rhythmic cardiac blood pumping has proposed for the first time. A smart material with cardiac-like behavior promotes the electrochemical kinetics through the self-regulation of stress under the variation of voltage in the redox reaction. Under the micro-stress field, a half-cell demonstrates a capacity of 119.1 mA h g−1 at 35 A g−1, and a 1.04 A h pouch cell shows an excellent energy density of 317.2 W h kg−1 (the retention is 90.2% after 500 cycles at 1C). Via further analysis of physicochemical characterizations and the sensor signal, the signal correlation of the mechanism between electrochemistry and stress was obtained. This work provides a strategy for accelerating the transmission rate of Na+ based on a stress field.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Biotechnology Journal
·
2024
·Vol. 19
·期 2
·2300390
·
IF 3.2
Yaqiong Guo,Yingying Xie,Jianhua Qin
Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their P app values and human Fa, demonstrating the capability of this model for drug absorption evaluation. Our findings highlight the potential of this pump-free organ-on-a-chip platform as a valuable tool for advancing drug development and enabling personalized medicine.
研究领域:
生物医学工程
·
药理学
·
组织工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Jiaojiao Tao,Yu Dong,Bingjie Wang,Teng Wang,Aijia Zhang,Shuang Li,Rui Chen,Yanguo Su,Tianze Jiang,Xia Zhao
Immunotherapy is a promising new approach for tumor treatment. However, its clinical application is hindered by insufficient immunogenicity, hypoxia, and immunosuppressive tumor microenvironment (TME). Here, oxygen pump microneedles (OPMNs) loaded with zinc-doped copper sulfide nanoflowers (ZCS NFs) and PD-L1 small interfering RNA (siPD-L1) (OPMNs-ZCS@siPD-L1) are developed for boosting tumor immunotherapy. OPMN-ZCS@siPD-L1 enhances tumor immunogenicity through ZCS NFs by inducing cuproptosis, reverses TME through siPD-L1, and promotes drug penetration, and ameliorates hypoxia through oxygen bubbles. More importantly, cuproptosis-induced mitochondrial DNA (mtDNA) together with Zn 2+ co-activate the STING pathway, triggering a robust immune response. OPMN-ZCS@siPD-L1 increases the sensitivity to cuproptosis and induces immunogenic cell death (ICD) in vivo and in vitro, which significantly inhibits tumor progression and metastasis. The novel strategy of “increasing the throttle” (cuproptopsis-mediated STING activation & ICD effect) combined with “releasing the brake” (PD-L1 inhibition & hypoxia improvement) provides a new approach for enhancing percutaneous tumor immunotherapy.
研究领域:
肿瘤学
·
免疫学
·
纳米医学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
·
2025
·
IF 10.9
Zhou Sha,Jing Li,Yuheng Song,Hongchuang Li,Hongmei Liu,Jiahui Fan,Xueyong Li,Xiang Fei,Meifang Zhu
Diabetic wounds, characterized by excessive exudate accumulation and dysregulated inflammation, pose a global healthcare challenge due to their persistent non-healing nature. Traditional therapies often fail to simultaneously address three critical healing requirements: precise exudate management, coordinated inflammation-immune modulation, and pro-regenerative microenvironment establishment. Here, we present an innovative self-pumping Janus fibrous dressing integrating curcumin-loaded polycaprolactone nanofibers with cotton gauze. Its gradient architecture enables spontaneous exudate drainage and dynamic microenvironment modulation, establishing an optimal milieu for accelerated wound healing. Comprehensive in vitro investigations validate the dressing’s multifunctionality, including sensitive colorimetric pH response, antibacterial properties (>99 % inhibition against S. aureus ), and antioxidant capabilities (>90 % DPPH radical scavenging). In vivo studies in a diabetic mice model demonstrate the dressing’s superior wound healing efficacy, with ∼47 % reduction in wound edge maceration, mitigated inflammatory responses, and enhanced angiogenesis compared to commercial cotton dressings. This novel approach provides a promising foundation for the development of advanced wound dressings tailored for the management and healing of chronic wounds.
研究领域:
生物材料
·
伤口愈合
·
纳米技术
·
免疫调节
·
药物递送
✓ 已验证
产品关联
DOI
高影响因子
Advanced Energy Materials
·
2024
·Vol. 14
·期 16
·2303912
·
IF 24.4
Peipei Wu,Peng Yang,Zhaoqi Liu,Guangzhao Huang,Xinglin Tao,Siyao Qin,Xuanyi Dong,Li Zheng,Hexing Li,Xiangyu Chen,Zhong Lin Wang
In recent years, liquid–solid triboelectric nanogenerator (LSTENG) has attracted great attention. However, its low average power density and long charging time limit the practical application of this technique. Here, for the first time, a liquid–solid triboelectric nanogenerator combined with external charge-pumping method (ECP-LSTENG) is proposed. Compared to traditional LSTENGs, a charge storage capacitor is added in this ECP-LSTENG to store the output charges from the pumping generator. Then, a charge extraction capacitor is formed by the spreading and shrinking processes of falling droplet, while the charging and discharging process between charge storage and extraction capacitors can generate displacement current output for external circuit. Meanwhile, it is found that a special charged droplets adhesion phenomenon happens at the liquid–solid interface, which seriously hinders the continuous output of ECP-LSTENG. To overcome this limitation, a special coaxial deceleration commutation component is designed to ensure continuous output of the ECP-LSTENG. Finally, the peak power density of a droplet (50 µL) reaches 231.8 W m −2 , surpassing the highest research record to date by 1.43 times. This ECP-LSTENG provides a different approach for the future development of large-scale integrated raindrop generators for efficient raindrop energy harvesting.
研究领域:
纳米技术
·
能量收集
·
可再生能源
·
智能材料
✓ 已验证
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2016
·
IF 1.879
Rong Chen,Ying Tian,Bingpeng Li,Feifei Huang,Caizhi Wang,Xufeng Jing,Junjie Zhang,Shiqing Xu
Nd 3+ /Ho 3+ co-doped silicate-germanate glass has been synthesized by high temperature melt-quenching method. Near infrared emission centered at 2 µm has been successfully obtained by incorporating Nd 3+ and Ho 3+ in present glass. The Judd-Ofelt intensity parameters Ω t (t=2, 4, 6), and radiative properties of Ho 3+ were calculated and discussed by using the Judd-Ofelt theory. The energy transfer processes and luminescence properties of Nd 3+ /Ho 3+ co-doped glasses were analyzed pumped by a conventional 808 nm laser diode . Desirable spectroscopic characteristics indicates that Nd 3+ /Ho 3+ co-doped silicate-germanate glass might be a good alternative matrix for 2 µm band mid-infrared laser.
研究领域:
光学材料
·
无机非金属材料
·
光子学
·
激光技术
·
固体物理
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2013
·Vol. 14
·期 1
·1802-1821
·
IF 2.464
Rongxin Qin,Kangkang Xiao,Bin Li,Weiwei Jiang,Wei Peng,Jiang Zheng,Hong Zhou
Fructus crataegi (hawthorn) is the common name of all plant species in the genusCrataegusof the Rosaceae family. In the present study, three monomers of (+)-catechin (C), (−)-epicatechin gallate (ECg) and (−)-epigallocatechin (EGC) were isolated from the hawthorn under the guide of antibacterial sensitization activity. The bioactivity of the composite fraction in enhancing the antibacterial effect of oxacillin against methicillin-resistantStaphylococcus aureus(MRSA) was greater than that of the individual monomer of the hawthorn extractin vitro. Two-fold dilution and checkerboard methods were used to analyze antibacterial activity and screen for the combination and proportion of monomers with the best bioactivity. The result showed that C (128 mg/L) combined with ECg (16 mg/L) had the greatest effect and the combination also reduced the bacterial load in blood of septic mice challenged with a sublethal dose of MRSA, increased daunomycin accumulation within MRSA and down-regulated the mRNA expression ofnorA,norCandabcA, three important efflux pumps of MRSA. In summary, C and ECg enhanced the antibacterial effect of β-lactam antibiotics against MRSAin vitroandin vivo, which might be related to the increased accumulation of antibiotics within MRSA via suppression of important efflux pumps’ gene expression.Keywords:fructus crataegi;(+)-catechin;(−)-epicatechin gallate;synergistic effect;MRSA;drug accumulation
研究领域:
药理学
·
微生物学
·
植物化学
·
传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）