TALANTA
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2017
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IF 4.162
Yong Hu,Hai-Long Wu,Xiao-Li Yin,Hui-Wen Gu,Rong Xiao,Li-Xia Xie,Zhi Liu,Huan Fang,Li Wang,Ru-Qin Yu
The aim of the present work was to develop a rapid and interference-free method based on liquid chromatography-mass spectrometry (LC-MS) for the simultaneous determination of nine B-group vitamins in various energy drinks. A smart and green strategy that modeled the three-way data array of LC-MS with second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating penalty trilinear decomposition (APTLD) algorithms was developed. By virtue of "mathematical separation" and "second-order advantage", the proposed strategy successfully solved the co-eluted peaks and unknown interferents in LC-MS analysis with the elution time less than 4.5 min and simple sample preparation. Satisfactory quantitative results were obtained by the ATLD-LC-MS and APTLD-LC-MS methods for the spiked recovery assays, with the average spiked recoveries ranging from 87.2–113.9% to 92.0–111.7%, respectively. These results acquired from the proposed methods were confirmed by the LC-MS/MS method, which shows a quite good consistency with each other. All these results demonstrated that the developed chemometrics-assisted LC-MS strategy had advantages of being rapid, green, accurate and low-cost, and it could be an attractive alternative for the determination of multiple vitamins in complex food matrices, which required no laborious sample preparation, tedious condition optimization or more sophisticated instrumentations.
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Aquaculture Reports
·
2024
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IF 3.2
Kangyuan Qu,Siyuan Xiong,Caiqian Huang,Xiaoxia Jiang,Lixia Tian,Shiwei Xie
Ornithine is an arginine family amino acid and is not incorporated into protein. This study evaluated its role in growth, immune response and acute NH 3 stress tolerance of Penaeus vannamei . Six diets with graded levels (0.6, 0.9, 1.2, 1.5, 1.8, 2.1 g kg −1 ) of L-ornithine were formulated, and fed the shrimp for 8 weeks. The growth of shrimp was significantly improved with the dietary ornithine levels increased from 0.6 to 1.5 g kg −1 . Feed efficiency, protein efficiency ratio and protein productive value showed a similar trend to the growth performance of shrimp. Whole body protein and muscle lipid of shrimp were significantly increased in shrimp fed 1.8 g kg −1 ornithine compared to those fed 0.6 g kg −1 ornithine. The contents of several amino acids were increased with the dietary ornithine increased to 1.5 g kg −1 . Elevated levels of ornithine increased the Nitric Oxide (NO) content and suppressed arginase activity in the hemolymph. The relative mRNA expression of superoxide dismutase , heat shock protein 70 , dorsal and caspase 3 were increased with the dietary addition of ornithine, while the mRNA level of arginase was decreased with the increasing dietary ornithine levels. After 9 h acute NH 3 stress, mortality of shrimp reduced with the increasing dietary ornithine levels. The relative mRNA levels of superoxide dismutase , heat shock protein 70 , dorsal and catalase were increased with the dietary addition of ornithine after the acute NH 3 stress. The second-order regression analysis of weight gain and survival after acute stress revealed that the suitable ornithine content in the shrimp diet was 1.4–1.5 g kg −1 .
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ELECTRONIC JOURNAL OF BIOTECHNOLOGY
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2025
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IF 2.5
Yi Li,Jiacheng Tang,Kaixiang Xin,Zongda Chen,Lele Zhao,Yifan Zhao,Yinbiao Xu,Pei Zhou,Yang Sun,Yupeng Liu,Hua Li
Background Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements. Results Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening. Conclusions Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, Pseudomonas poae have been obtained, which designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 h, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.
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Cell Reports
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2025
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IF 6.9
Zeng Zhang,Quan Guo,Zhihan Yang,Yukai Sun,Shuaiming Jiang,Yangli He,Jiahe Li,Jiachao Zhang
Sarcopenia significantly diminishes quality of life and increases mortality risk in older adults. While the connection between the gut microbiome and muscle health is recognized, the underlying mechanisms are poorly understood. In this study, shotgun metagenomics revealed that Bifidobacterium adolescentis is notably depleted in individuals with sarcopenia, correlating with reduced muscle mass and function. This finding was validated in aged mice. Metabolomics analysis identified nicotinic acid as a key metabolite produced by B. adolescentis , linked to improvements in muscle mass and functionality in individuals with sarcopenia. Mechanistically, nicotinic acid restores nicotinamide adenine dinucleotide (NAD+) levels in muscle, inhibits the FoxO3/Atrogin-1/Murf-1 axis, and promotes satellite cell proliferation, reducing muscle atrophy. Additionally, NAD+ activation enhances the silent-information-regulator 1 (SIRT1)/peroxisome-proliferator-activated-receptor-γ-coactivator 1-alpha (PGC-1α) axis, stimulating mitochondrial biogenesis and promoting oxidative metabolism in slow-twitch fibers, ultimately improving muscle function. Our findings suggest that B. adolescentis -derived nicotinic acid could be a promising therapeutic strategy for individuals with sarcopenia.
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EMBO JOURNAL
·
2024
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IF 9.4
SunHongzheng,HanLongsen,GuoYueshuai,AnHuiqing,WangBing,ZhangXiangzheng,LiJiashuo,JiangYingtong,WangYue,SunGuangyi,ZhuShuai,TangShoubin,GeJuan,ChenMinjian,GuoXuejiang,WangQiang
Phosphorylation is a key post-translational modification regulating protein function and biological outcomes. However, the phosphorylation dynamics orchestrating mammalian oocyte development remains poorly understood. In the present study, we apply high-resolution mass spectrometry-based phosphoproteomics to obtain the first global in vivo quantification of mouse oocyte phosphorylation. Of more than 8000 phosphosites, 75% significantly oscillate and 64% exhibit marked upregulation during meiotic maturation, indicative of the dominant regulatory role. Moreover, we identify numerous novel phosphosites on oocyte proteins and a few highly conserved phosphosites in oocytes from different species. Through functional perturbations, we demonstrate that phosphorylation status of specific sites participates in modulating critical events including metabolism, translation, and RNA processing during meiosis. Finally, we combine inhibitor screening and enzyme-substrate network prediction to discover previously unexplored kinases and phosphatases that are essential for oocyte maturation. In sum, our data define landscape of the oocyte phosphoproteome, enabling in-depth mechanistic insights into developmental control of germ cells.
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CHEMICAL ENGINEERING SCIENCE
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2024
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IF 4.1
Xiaoxiao Liang,Yanru Wu,Yuehua Deng,Xingye Zeng,Shufeng Shan,Yanbin Jiang,Huaiyu Yang
Multi-component crystal based on supramolecular self-assembly is an effective strategy to modify the physicochemical properties of poorly water-soluble drugs. In this study, eight novel multi-component crystals of Osimertinib (AZD) were firstly synthesized under the guidance of theoretical analysis. Five single crystals were successfully obtained, and the AZD-FMS cocrystal shows a distinctive structure wherein free FMS filled in the skeleton, which may be crucial for maintaining morphology. Analysis of crystal structures and Hirshfeld surfaces indicated the involvement of N−H···O and N···H−O hydrogen bonds in the multi-component crystals. Owing to robust intermolecular interactions, multi-component crystals exhibited improved solubility/dissolution rate compared to pure AZD. Multivariate analysis revealed coformers and strong solute–solvent interactions determined the solubility/dissolution rate. Multi-component crystals showed superior stability in solution and low humidity. Moreover, AZD-FSM increased 5.2-fold anti-tumor activity over AZD, which is anticipated to enhance efficacy, reduce drug toxicity, combat drug resistance, and transform from laboratory to clinical application.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Junli Li,Guoqiang Xiang,Lijun He,Xiuming Jiang,Renyong Zhao
B, N-co-doped carbon dots (B, N-CDs) were prepared using hydrothermal method with 3-amino phenylboronic acid (3-APBA) as a precursor, yielding 82.5 %. The as-prepared B, N-CDs demonstrated highly stable fluorescence emission with a fluorescence quantum yield (FQY) of 51.4 % under various experimental conditions including 4 M NaCl solution, 120 days of storage, and solution temperature of 100 °C. Based on the boronate affinity between boric acid groups on the surface of B, N-CDs, and the cis -diol structure of ascorbic acid (AA), a novel fluorescence assay for AA was established with a detection limit of 41 nM. Additionally, based on the redox reaction between hydroperoxide (H 2 O 2 ) and boric acid groups on B, N-CDs, a fluorescence assay for H 2 O 2 with a detection limit of 4.5 nM was developed. The proposed fluorescent assay methods were successfully applied for the determination of AA and H 2 O 2 traces in food samples, and the spiking recoveries were in the range of 82.5 % −115.75 %.
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Nature Protocols
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2025
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IF 16
Wu Kejia,Zhou Lei,Liu Laiyan,Yang Min,Li Jiang,Ma Shichun,Sousa Diana Z.,Cheng Lei
Methanogenic archaea play a crucial role in the global carbon cycle and in climate change. Recent metagenomic sequencing has revealed a considerable number of (putative) H 2 -dependent methylotrophic methanogens (HMMs) across the archaeal tree and in diverse environments. Traditional isolation methods, such as dilution-to-extinction and roll-tube techniques, fail to cultivate fastidious HMMs. Here, we describe a four-stage isolation strategy designed to selectively isolate HMMs by using a flexible combination of methods to systematically reduce microbial complexity to a pure culture. In the initial stage, the growth conditions for the target HMM were optimized through closed-batch cultivation encompassing >50 conditions. Second, HMM-containing cultures were serially diluted in 96-well plates combined with substrate limitation to eliminate non-target archaea. In stage 3, the bacterial diversity in the culture was further decreased to a single bacterium by treatment with antibiotics and lysozyme. Finally, a last bacterial contaminant was removed by repeated addition of antibiotic mixtures and successive dilution transfers, leading to the successful isolation of the first pure culture of Methanosuratincola petrocarbonis LWZ-6, an HMM of the phylum Thermoproteota. This protocol also describes molecular methods, including 16S rRNA gene amplicon sequencing, metagenome sequencing and quantitative PCR, to track microbial community shifts and assess the growth advantage of the target HMM, enabling monitoring of the stepwise elimination of non-target microorganisms and ultimately confirming the purification of the target HMM. The duration of the protocol will vary for different HMMs depending on their substrate utilization, growth rate and method selection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Jinsong Wang,Farzad Seidi,Xiaotong Shi,Chengcheng Li,Yang Huang,Huining Xiao
The development of self-healing ecofriendly coatings for anticorrosion applications provides stronger protection than passive coatings and mitigates the environmental pollution resulting from petroleum-derived coatings. Due to its intrinsic anticorrosion characteristics, lignin is a viable precursor for the formulation of self-healing anticorrosion coatings. However, the structural rigidity of lignin and the lack of self-healing functionalities in pristine lignin-based coatings hinder the comprehensive application of lignin in this field. Accordingly, we introduced Diels-Alder (DA)-based self-healing functions in the lignin-based coatings and meanwhile, the coatings were chemically conjugated with corrosion inhibitors to further improve the anticorrosion performance. A thin layer (16 μm) of the coating increased the E corr from −480 mV (for bare steel) to +91 mV and reduced the corrosion rate (CR) >4000 times. The dual self-healing properties of the coating are attributed to the synergistic action of the DA adduct and the conjugated corrosion inhibitor. Practically, in response to the emergence of microcracks in the coating structure, trace amounts of the corrosion inhibitor are released into the microcracks to form a protective layer on the unveiled metal surface. Simultaneously, DA adducts undergo reversible reactions at specific temperatures (50–120 °C) promoting complete restoration of the microcrack.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Jia Xu,Hongjian Ji,Guozhe Zhang,Jin Cheng,Qin Shi,Jie Zhang
Luteolin, a bioactive flavonoid known for its anti-inflammatory, antioxidant, and anticancer properties, is limited in its clinical application due to poor water solubility and low bioavailability. In this study, we applied an integrated approach combining virtual screening and experimental validation to identify potential coformers that can form cocrystals with luteolin, improving its physicochemical properties. A total of 28 coformers were analyzed using virtual screening, with predictions based on hydrogen bond strength. From this, 12 promising coformers were selected for experimental trials. Our experimental results led to the successful synthesis of five novel luteolin cocrystals with 4,4′-bipyridine, l -ascorbic acid, nicotinic acid, and isonicotinic acid. Notably, the coformers that formed cocrystals in the experiments ranked highly in the virtual screening results, demonstrating the feasibility of the virtual cocrystal screening method. Notably, all synthesized cocrystals exhibited substantially enhanced solubility profiles, achieving a maximum 4.51-fold increase in solubility relative to pure luteolin. These findings not only validate the virtual screening approach but also highlight its potential applications in pharmaceutical cocrystal design to overcome solubility and bioavailability challenges in drug development.
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LANGMUIR
·
2025
·
IF 3.9
Linghui Meng,Jie Jiang,Xuesong Peng,Lin Zhu,Ruopeng Li,Yaqiang Li,Maozhong An
Developing a cyanide-free gold electrodeposition system is significant for replacing the cyanide electroplating gold plating system, which is highly harmful to the environment and toxic to human health. In our study, a cyanide-free system for electrodepositing gold (Au) coatings was proposed with AuCl as the main salt and 5,5-dimethyl hydantoin (DMH) as the Au (I) complexing agent. Through theoretical calculations and electrochemical test screening, 4,6-dimethyl-2-mercaptopyrimidine (DMP) is suitable for DMH-based gold electrodeposition and was selected as an electroplating additive. The role of DMP in gold electrodeposition was further studied. In particular, the adsorption–coordination mechanism was proposed to elucidate the role of DMP, based on calculations of the Bader charge density difference for DMP adsorbed on the gold surface and the interaction between DMP and Au+. Electrochemical tests such as cyclic voltammetry and chronoamperometry were utilized to study the impact of varying DMP concentrations on gold electrodeposition behavior. Chronoamperometric analysis indicates that Au electrodeposition obeys a 3D diffusion-controlled progressive nucleation process, independent of DMP concentration and applied potential, while the presence of DMP markedly suppresses grain growth. The addition of DMP significantly improved the surface morphology and brightness of the gold coating, resulting in a smooth and dense layer with a golden-yellow appearance. XRD results showed that the addition of DMP reduced the grain size of the Au coating from 22.1 to 15.7 nm, with the gold coating primarily growing along the Au (111) plane. The XPS test results show that the purity of the gold plating layer is high, and there is no inclusion of additives in the coating.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Zhipeng Zhu,Yuqian Xiang,Xiaohui Yan,Chenglong Shi,Cheng Chen,Hongxing Liu,Bin Wang,Yanshuo Li,Dapeng Wu
Crosslinked and uncrosslinked polyacrylamide stationary phases were prepared on porous silica gel (SiL-PAAm) by atom transfer radical polymerization (ATRP), and their hydrophilic interaction liquid chromatography (HILIC) separation performance and stability were evaluated systematically. After aminosilylation and esterification with 2-bromoisobutyryl bromide on silica gel, surface-initiated ATRP with acrylamide or acrylamide/N,N’-methylenebisacrylamide mixtures at molar ratios of 1:0.1 and 1:0.2 was carried out, and SiL-PAAm-0, SiL-PAAm-10, and SiL-PAAm-20 HILIC materials were obtained. SiL-PAAms were characterized by FT-IR, TGA and nitrogen adsorption–desorption isotherms. It was confirmed that these three SiL-PAAms had very similar specific surface area of 130–143 m 2 g −1 , pore size of 8.67–8.89 nm, and PAAm bonding amount of 177–181 mg g −1 . Their HILIC performances were evaluated and compared by separating nucleosides, vitamins, sugars, and amino acids, respectively. It was found that SiL-PAAms exhibited significantly stronger retention and higher separation selectivity than both bare silica and commercial amide columns. However, an excessive increase in the crosslinking degree also led to a decrease in retention strength. Additionally, the SiL-PAAm-10 and SiL-PAAm-20 exhibited significantly reduced column bleeding and enhanced reproducibility, far surpassing both SiL-PAAm-0 and commercial amide columns. Generally, the suitably crosslinked PAAm by ATRP has been proven as one highly efficient and robust HILIC stationary phase for the chromatographic separation of a wide range of polar compounds.
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危险品化学品经营许可证（带存储）