JOURNAL OF APPLIED MICROBIOLOGY
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2019
·Vol. 126
·期 1
·102-112
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IF 2.683
Lin W.,Yuan D.,Deng Z.,Niu B.,Chen Q.
ObjectivesIn our previous research, we have developed a new combination disinfectant, glutaraldehyde‐didecyldimethylammonium bromide (GD). It was verified that GD had a strong effect on both Escherichia coli and Staphylococcus aureus. In this work, Candida albicans was selected as an object, and it could be killed by GD. We aimed to investigate the cellular and molecular mechanism of GD effecting on C. albicans.Methods and ResultsThe results of sterilization experiment indicated that GD was effective on C. albicans. Flow cytometry and atomic absorption spectrometry were applied to detect cell membrane damage of C. albicans. Luciferase reaction and Bradford method were carried out to detect ATP content and protein quantitation. Transmission electron microscopy was used for intracellular organelles morphological observation. In order to study changes in mitochondrial membrane potential, Rh 123 was used as an indicator. DNA conformation analysis was performed by molecular modelling and circular dichroism. The results indicated that membrane permeability was increased rapidly owing to GD effect, and the leaked K+ and Mg2+ were about 12·1 and 12·4 times those of the control, respectively, at 10 min after GD treatment. Simultaneously, ATP and protein also leaked rapidly out of the cell. Mitochondrial membrane potential was destroyed, succinic dehydrogenase activity was significantly decreased and DNA conformation was changed because of GD action.ConclusionsGlutaraldehyde‐didecyldimethylammonium bromide disinfected C. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K+, Mg2+, ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death.Significance and Impact of the StudyThis study focused on the cellular and molecular mechanism of GD as a disinfectant against C. albicans. It is important to provide theoretical support to GD against Candida albicans in practical application.
研究领域:
微生物学
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分子生物学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2018
·
IF 6.578
Junbin Liao,Jiajie Zhu,Shanshan Yang,Nengxiu Pan,Xinyan Yu,Chao Wang,Jun Li,Jiangnan Shen
Developing highly ion conductive and desirable electrodialysis (ED) performance anion exchange membranes (AEMs) is of great significance. In this work, we report a strategy for fabricating novel AEMs based on a series of fluoro-methyl poly(arylene ether ketone)s having long-side-chain imidazolium groups for ED. Having hydrophilic conductive flexible side-chain imidazolium groups and rigid hydrophobic backbone within AEM matrix, the optimized AEM (PAEK-60-im) shows low water uptake of 24.9% at 80 °C and desirable ion conductivity of 22.01 mS cm –1 at 30 °C. In NaCl removal application of ED, PAEK-60-im shows the NaCl removal ratio of 69.66% within 120 min, outperforming commercial AEM-Type II (68.98%). Accordingly, it has a higher current efficiency of 83.7% but lower energy consumption of 5.9 kWh kg –1 , compared with the commercial one (82.0%; 6.6 kWh kg –1 ). In particular, in selectrodialysis application, possibly owing to the synergistic effect of pore-size sieving effect resulting from macro-separation morphology and hydration energy difference of SO 4 2– ions (–1000 kJ mol –1 ) and Cl – ions (–317 kJ mol –1 ) in feeding solution, PAEK-60-im shows significantly superior monovalent anion perm-selectivity (Cl − /SO 4 2− ) of 7.70 at 30 min, relative to commercial anion-selective Neosepta ACS (5.27) and many other reported mono-valent anion selective AEMs having elegant surface modified layers. The superior ED performance of the homogeneous PAEK-60-im is suggestive of its potential separation application.
研究领域:
材料科学
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化学工程
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电化学
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膜技术
✓ 已验证
产品关联
DOI
高影响因子
Energy Technology
·
2019
·Vol. 7
·期 4
·1800981
·
IF 3.163
Bangwei Deng,Hao Wang,Xiang Li,Yan Ding,Daming Sun,Jun Ma,Mianzhong Chen,Tao Chen,Feng Gao,Meizhen Qu,Gongchang Peng
Tris(trimethylsilyl)phosphite (TMSPi) has been reported to be an excellent electrolyte additive. However, scarce reports exist on the effect of the charge cutoff potential on the cyclic stability using high-performance LiNi 0.6 Co 0.2 Mn 0.2 O 2 (NCM622)–mesocarbon microbead (MCMB) full cells. Herein, a high-loading and repeatable NCM622–MCMB coin-type full cell is successfully prepared, and the role of TMSPi on such a full cell at charge cutoff potentials of 4.25 and 4.45 V is studied. Theoretical calculations and detailed analyses reveal that it is oxidized in the first charging process due to its unsaturated P—O bonds, thus forming a stable P—O—F-rich cathode electrolyte interphase and preventing severe decomposition of the electrolyte over NCM622 during long-term cycling. In addition, the P—O and Si—O bonds of TMSPi both display HF-scavenging ability. Moreover, TMSPi may react directly with the LiPF 6 -based electrolyte to form some intermediate products that can be reduced on the MCMB electrode, thereby modifying the solid electrolyte interphase and improving the stability of MCMB during lithium intercalation.
研究领域:
电化学
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材料科学
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能源存储
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锂离子电池
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界面化学
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电极材料
✓ 已验证
DOI
高影响因子
POLYMER TESTING
·
2022
·
IF 4.931
Yang-he Mo,Hui Wang,Shao-hua Jin,Ke-lin Peng,Zi-ming Yang,Pu-wang Li,Yu Chen
Fast curing hydrogels are highly valuable in the skin care industry. Multi-arm polyethylene glycol (PEG) is a commonly used crosslinking agent for the preparation of fast curing hydrogels. However, the hydrogel preparation using multi-arm PEG crosslinking agents usually requires the end group modifications of reactants, which makes the preparation process complicated and produces hydrogels with low curing rates. In the present study, a fast in situ curing hydrogel, 4-arm-PEG-succinimidyl carbonate/carboxymethyl chitosan (PEG-SC/CMCS), was prepared using 4-arm-PEG-SC as the crosslinking agent and carboxymethyl chitosan (CMCS), a derivative of natural polysaccharide with excellent comprehensive properties, as the framework without any end groups modified. The structure and properties of the prepared hydrogel were characterized by FTIR, XRD, SEM, rheology and swelling kinetics. The effects of reaction conditions on the structure and properties of hydrogel were investigated. The PEG-SC/CMCS hydrogel exhibited pH-sensitive swelling behaviors. Its application potential in skin care products is demonstrated with its excellent sustained-release performance of Vitamin C, a model drug for skin treatment, and high adsorption capacity of heavy metal ions that are easily deposited on skin surface. Our work has provided a new strategy for building multifunctional gel materials for skin care and offered a PEG-SC/CMCS hydrogel with great application potentials.
研究领域:
生物材料
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高分子化学
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药剂学
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皮肤科学
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组织工程
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药物递送系统
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环境毒理学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2018
·
IF 2.559
Peng Zhang,Menghuan Tang,Qin Huang,Guanjian Zhao,Ning Huang,Xiang Zhang,Ying Tan,Yuan Cheng
Mesoporous silica nanoparticles (MSNPs) of a small diameter were loaded with the anticancer drug temozolomide (TMZ), coated with polydopamine (PDA), and conjugated with Asn-Gly-Arg (NGR) for use in the treatment of glioma. The accumulation of NGR-MSNPs in C6 cells was shown to be higher than that of unmodified MSNPs. Anticancer drugs can cause autophagy in tumor cells, whereas autophagy inhibitors can block this reaction and enhance the therapeutic effect of the drugs. In this study, we demonstrated that MSNP-TMZ-PDA-NGR had stronger autophagy- and apoptosis-inducing effects in C6 cells than TMZ alone, and its anticancer effect was further enhanced when combined with autophagy inhibition. These results demonstrate that the combination of targeting vehicles and autophagy inhibitors may have research value in the treatment of gliomas.
研究领域:
纳米技术
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药学
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肿瘤学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）