MOLECULES
·
2025
·Vol. 30
·期 18
·3669
·
IF 4.6
Zirun Zhao,Rou Liu,Jiawen Yu,Yunlong Liu,Mingchun Li,Qilin Yu
Soil saline–alkali stress is a major problem faced by global agriculture, and there is an urgent need to develop efficient amelioration strategies. While both probiotics and plant stress-resistant molecules play critical roles in the alleviation of crop stress, their efficient retention in crop rhizosphere regions remains a great challenge. In this study, the nanocarrier ZIF-8@SPBP@betaine (ZSBet) was constructed by introduction of the synthesized polysaccharide-binding protein (SPBP) and the stress-resistant molecule betaine to the metal–organic framework ZIF-8. During co-incubation, the probiotic Novosphingobium capsulatum and ZSBet efficiently bound together to form ZSBet + Novo co-assemblies, i.e., the integrated protein-ZIF-8-probiotic complexes mediated by polysaccharide-receptor recognition, which exhibited strong root-binding abilities. Microbiome analysis revealed that ZSBet + Novo reduced the α-diversity of rhizosphere bacteria and increased the absolute abundance of biofilm formation-related bacteria, e.g., Novosphingobium , Sphingobium , and Lactococcus . During wheat cultivation in saline–alkali soil, ZSBet + Novo reduced soil pH by 0.63 units, decreased soil salt content by 0.11 g/kg, and increased soil nutrient levels. Furthermore, the co-assembly enhanced the wheat grain number by 145.05% and reduced root malondialdehyde and proline contents by 42.00% and 39.13%, respectively. This study provides a new strategy for improving crop resistance under saline–alkali stress in combination with nanotechnology and synthetic biology.
研究领域:
环境微生物学
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纳米生物技术
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植物生理学
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土壤修复
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合成生物学
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作物抗逆育种
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分子生态学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Junjie Wang,Hui Zhou,Yang Li,Yeshu Mu,Jinghua Li,Chen Hong,Wen Luo
A novel series of benzo[ cd ]indol-2(1 H )-one-donepezil (BIO-DPZ) hybrids (5a-m, 6a-k) was designed, synthesized and evaluated as cholinesterase inhibitors and living cell imaging agents. These compounds were potent inhibitors of both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Compound 6a exhibited the strongest inhibitory activity against AChE with an IC 50 value of 0.16 μM. Compound 6i had the strongest inhibition of BuChE with an IC 50 value of 5.37 μM. Kinetic and molecular modeling studies showed that 6a bound both catalytic active site and peripheral anionic site of AChE. Compounds 6a and 6i both showed lower SH-SY5Y, HepG2 and HL-7702 cytotoxicity than the mitoxantrone. Promisingly, the most active compound, 6a, was successfully used in living SH-SY5Y cell imaging and had predictable lipophilicity and blood-brain-barrier permeation properties. These hybrids should undergo further systematic study in the treatment of Alzheimer's disease.
研究领域:
药理学
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神经科学
·
药物化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yu Han,Yan Yang,Yuling Ma,Daxin Liang,Liping Wen,Jun Ma,Wei Wang
As lithium is the cornerstone of green energy development, it is crucial to realize a low environmental impact and efficient lithium extraction process. Ion-sieve adsorption is the most widely used method to extract liquid lithium resources, but this method is only efficient under alkaline conditions for H + and Mg 2+ competing adsorption. Conventional methods are often accompanied by the consumption of quantities of alkali, the generation of solid waste, and the acidification of liquid lithium resources. To address these issues, a selective ion-channel adsorbent was constructed. The composition comprises an ion sieve adsorbent and an organic carrier with a zwitterionic quaternary ammonium base group. This group storages OH - in situ, hinders H + diffusion, slows down Mg 2+ diffusion, and accelerates Li + diffusion by relying on the difference in binding energies, which reduces the competing adsorption and avoids acidification and solid waste generation. The saturated adsorption capacity (21.38 mg/g) and selectivity of the adsorbent are 4.7 and 24 times higher than that of conventional ion-sieve adsorbent under neutral conditions respectively. The dosage of alkali is 1/256 of the traditional method, the effluent remains neutral and no solid waste is generated. This study presents an environmental and effective adsorbent for lithium extraction.
研究领域:
材料科学
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环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Jia-bin Su,Wen-long Wu,Chang-E Dong,Shun Yang,Yuan-yuan Feng,Tian Qin,Ke-qi Chen,Jing-jing Qian,Jing-pei Zou,Yu-Han Liu,Shan-ming Liu,Wei-Wei Liu,Da-Hua Shi
Sixteen new 1,3,5-triazine-quinoline derivatives were designed and synthesized and evaluated as cholinesterase inhibitors. The structure of 1,3,5-triazine-quinoline derivatives were confirmed by IR, NMR, HRMS and single crystal X-ray diffraction experiments. Compound 5j had a monoclinic crystal system and P2 1 /c space group. Compound 5k had a monoclinic crystal system and C2/c space group. The cholinesterase inhibitory activity of the synthetic compounds were measured using the colorimetric Ellman's method. Compound 5 g showed best acetylcholinesterase inhibitory activity with IC 50 value of 10.82 μM and butyrylcholinesterase inhibitory activity with IC 50 value of 0.046 μM. Molecular docking showed that the compound 5 g could interacted with the active site of butyrylcholinesterase.
研究领域:
药物化学
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有机化学
·
生物化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Bin Jiang,Jiayun Guan,Yilin Lv,Chaojie Yang,Minfeng Yang,Xinyu Shen,Bin Xu,Qi Wang,Yuling Qin,Li Wu
The cholinergic hypothesis constitutes a pivotal theoretical framework for elucidating the pathological mechanisms underpinning Alzheimer's disease (AD). Quantitative assessment of cholinesterases (ChEs) and NAD(P)H levels in neuronal cells, particularly within the septo-hippocampal pathway, is imperative for the comprehensive study of the cholinergic system. In this study, we designed and synthesized a dual-channel, dual-enzyme-responsive fluorescent indicator that was chemically optimized for detecting levels of ChEs and NAD(P)H in neuronal cells. Biological experiments demonstrated that this indicator can sensitively and reliably visualize the dynamics of intracellular ChEs and NAD(P)H following stimulation. The corresponding ChEs activities and NAD(P)H concentrations demonstrated a linear correlation with fluorescence intensity, reaching equilibrium within a few minutes. This innovative and precise technique for visualizing ChEs and NAD(P)H dynamics with high spatial resolution during the progression of AD is expected to serve as a robust tool for investigating alterations in the cholinergic nervous system, including changes in acetylcholine neurotransmission and neuronal energy metabolism.
研究领域:
神经科学
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生物化学
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分子生物学
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医学研究
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荧光成像
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2025
·
IF 2.9
Siyu Hou,Xiya Peng,Qin Yu,Shufang Gao,Lu Li,Jian Luo
Eutectogels have emerged as versatile materials for wearable electronics, optical sensors, and biomedical applications. This study introduced the first investigation of microenvironmental basicity in poly(vinyl alcohol)/choline chloride (PVA/ChCl) eutectogels using lumichrome as a fluorescent probe. The incorporation of ChCl was demonstrated to enhance the microbasicity of PVA films, as evidenced by the significant promotion of lumichrome deprotonation. In contrast, no deprotonation occurred in deep eutectic solvents ethaline [n(ChCl):n(ethylene glycol) = 1:2], glyceline [n(ChCl):n(glycerol) = 1:2], and ChCl–water mixtures [n(ChCl):n(H2O) = 1:3 and 1:4]. Therefore, the proton acceptor in PVA/ChCl eutectogels was bound water, the same as in PVA films. Differential scanning calorimetry (DSC) experiments determined a decrease of water content in PVA films after ChCl incorporation, supporting the increased proportion of bound water in PVA/ChCl eutectogels. Our findings not only certified the enhanced microbasicity of bound water but also established a simple yet effective strategy to tune PVA films’ acid–base properties through ChCl loading.
研究领域:
材料科学
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高分子化学
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软物质物理
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传感器
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Huachun Liu,Yanfang Cheng,Hongwei Zhou,Xinhong Pan,Rongfei Peng,Zhijun Bai,Juntao Li,Lei Tan
Arsenic is a widely studied toxic element that exists in various species with different oxidation states and forms in the environment and biological systems. The different physicochemical properties, environmental behaviors, and toxicities of these arsenic species make speciation analysis essential for environmental monitoring and human health risk assessment. In this study, we demonstrated the determination and monitoring of six arsenic species, including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenocholine (AsC), and arsenobetaine (AsB) in seafood using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). We optimized the chromatographic condition and sample preparation for the separation and extraction of arsenic species, and the method was validated with accuracy and reproducibility using certified reference materials and actual samples. Baseline separation of the arsenic species was achieved within 12 min, and oven heat extraction was selected for sample preparation. The method showed excellent linearity for arsenic species from 0.1 to 100 μg L−1, with recoveries ranging from 92% to 123%, intra-day precision of 0.9–5.0%, and inter-day precision of 1.1–19.4%. The method was further used to determine the arsenic species in 109 mollusks, 195 crustaceans, and 195 marine fish samples from Guangdong, China. Distinctive patterns of arsenic species distribution were observed among different seafood categories. AsB was consistently the predominant species across all samples, with a detection frequency higher than 97.9% and contributed to more than 90% of total arsenic, other arsenic species showed marked variations in their occurrence and concentrations.
研究领域:
分析化学
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环境科学
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食品安全
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 2
·119
·
IF 5.118
Baojian Zhang,Maomao Ren,Yang Xiong,Haonan Li,Yong Wu,Ying Fu,Dongting Zhangsun,Shuai Dong,Sulan Luo
α-Conotoxin TxIB, a selective antagonist of α6/α3β2β3 nicotinic acetylcholine receptor, could be a potential therapeutic agent for addiction and Parkinson’s disease. As a peptide with a complex pharmacophoric conformation, it is important and difficult to find a modifiable site which can be modified effectively and efficiently without activity loss. In this study, three xylene scaffolds were individually reacted with one pair of the cysteine residues ([1,3] or [2,4]), and iodine oxidation was used to form a disulfide bond between the other pair. Overall, six analogs were synthesized with moderate isolated yields from 55% to 65%, which is four times higher than the traditional two-step oxidation with orthogonal protection on cysteines. The cysteine [2,4] modified analogs, with higher stability in human serum than native TxIB, showed obvious inhibitory effect and selectivity on α6/α3β2β3 nicotinic acetylcholine receptors (nAChRs), which was 100 times more than the cysteine [1,3] modified ones. This result demonstrated that the cysteine [2,4] disulfide bond is a new modifiable site of TxIB, and further modification can be a simple and feasible strategy for the exploitation and utilization of α-Conotoxin TxIB in drug discovery.
研究领域:
药理学
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神经科学
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药物化学
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DOI
高影响因子
Waste and Biomass Valorization
·
2018
·Vol. 10
·期 11
·3343-3350
·
IF 1.874
Chen Cheng,Jiang Linfeng,Ma Guanfeng,Jin Dongxue,Zhao Lisha,Ouyang Xinping
Abstract Lignin removal from lignocellulosic materials plays a great role in the utilization cellulose with high efficiency. In order to improve the quality of reconstituted tobacco sheet, lignin existed in tobacco stem was removed by enzymatic hydrolysis with laccase enhanced by weak alkali treatment together with the addition of Tween 80 into laccase treatment system. It is found that lignin was partially removed by laccase. The treatment of tobacco stem with NaHCO 3 resulted in the dissolution of lignin, and hence increased the porosity and specific surface area of the pretreated tobacco stem. Consequently, laccase could access to the tobacco stem more completely. The surface activity and dispersion of Tween 80 improved laccase activity, solubilization of degraded products of lignin, and contact interface between laccase and tobacco stem. The synergistic action of laccase, weak alkali and Tween 80 improved the ratio of lignin removal significantly, reaching as high as 40.3%. Graphical Abstract
研究领域:
生物化学
·
植物学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2020
·
IF 3.69
Wei Zhang,Mengjiao Wang,Huijie Song,Chengfeng Gao,Dongmei Wang,Huiming Hua,Yingni Pan,Xiaoqiu Liu
Ethnopharmacological relevance Evodiae Fructus (EF), the traditional Chinese medicine , has been typically used to treat headache, abdominal pain, hernias, and menorrhagia for thousands of years. It is a mild toxicity herb-medicine listed in Sheng Nong's Herbal Classic . Recently, EF was reported to have toxicity or no toxicity in some investigations. Toxicity and approaches to reducing toxicity of EF are of great interest. Limonin (LIM), a major triterpenoid component of EF, also had various pharmacological activities such as anti-inflammatory, anticancer, and antioxidant effects. However, little attention was paid to the role of LIM in EF-induced hepatotoxicity. Aim of study The study aimed to address the problem of controversial hepatotoxicity of EF, evaluate the role of CYP3A4 inducer/inhibitor in EF-induced hepatotoxicity and disclose the effect of LIM in EF-induced hepatotoxicity. Materials and methods The chemical compositions and hepatotoxicity of small flower EF (SEF), medium flower EF (MEF), big flower EF (BEF) and the “organ knock-out” samples (the shell and seed part of BEF) were investigated. Simultaneously, C57BL-6 mice were randomly divided into four groups, which were given orally administered BEF, BEF in combination with dexamethasone (DEX), BEF in combination with ketoconazole (KTC), and BEF in combination with LIM, respectively. Results In this study, more alkaloids and less LIM were detected in BEF compared with the compounds in SEF and MEF. Furthermore, we found that BEF group induced hepatotoxicity whereas no hepatotoxicity was observed in SEF and MEF groups. In addition, no LIM was detected in the shell part of BEF and five alkaloids were not detected in the seed part of BEF. Correspondingly, the shell part of BEF group induced hepatotoxicity whereas no hepatotoxicity was observed in the seed part of BEF group. It was also found that the BEF-induced hepatotoxicity was remarkably exacerbated when the mice were pretreated with DEX whereas the BEF-induced hepatotoxicity could be reversed by pretreatment with KTC or LIM. Conclusions Based on the results in this study, the misuse of BEF but not SEF and MEF could produce hepatotoxicity. The hepatotoxicity difference of different categories of EF might be associated with the relative contents of alkaloids and LIM. In addition, the results demonstrated that CYP3A4 inducer aggravated BEF-induced hepatotoxicity and LIM attenuated its hepatotoxicity.
研究领域:
民族药理学
·
毒理学
·
药理学
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DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 20
·3749-3761
·
IF 3.756
Mengya Li,Dandan Han,Junbo Gong
Uric acid (UA) is the end product of purine metabolism and is widely found in nature. Excess uric acid will result in the deposition of pathological uric acid crystals in the kidney. However, few studies have focused on the effects of components in the human body on the crystallization of uric acid. Herein, we found multiple effects of alkali metals (Na+, K+) on uric acid dihydrate (UAD) crystal growth, which is one of the main components of urinary stones. Optical microscopy (OM) and scanning electron microscopy (SEM) were employed to detect the crystal morphology, and long rod-like crystals and hierarchical crystals were found in the presence of Na+ and K+, respectively. Powder X-ray diffraction (PXRD) and micro-Raman spectroscopy verified that the crystal structure remained unchanged in the presence of ions. In addition, single-crystal growth experiments revealed that Na+ inhibited growth along [010] and [100], and K+ promoted growth along [100] but suppressed growth along [010]. The step growth of the UAD crystal was measured by atomic force microscopy (AFM), which indicated the inhibition of crystal growth by Na+ due to kink blocking, yet the promotion of crystal growth by K+ resulted from rough growth. Our studies showed that ions in the human body not only can maintain electrolyte balance but also can affect the crystallization of pathological crystals, which provides a suggestion for a rational diet for patients with hyperuricemia and urinary stones.
研究领域:
生物化学
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医学
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DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 10
·e4914
·
IF 3.2
Lingjie Hou,Linlin Wang,Chenhua Deng,Pengyue Jin,Chaochao Wen,Wenjia Zhang,Wenting Liang
Ca 2+ ion as a second messenger in signaling pathway plays many vital roles in many biological phenomena. Thus, it is of significance for developing effective probes to detect Ca 2+ ion specifically. Herein, a new Schiff base fluorescent probe FPH , fluorescein monoaldehyde (2-aminomethylpyridine) hydrazone, was designed and synthesized to identify Ca 2+ in DMSO aqueous solution. The probe FPH revealed significant responses to Ca 2+ with a fluorescence enhancement at 540 nm, exhibiting an evident fluorescence change from ultraweak luminescence to bright green. Otherwise, the FPH displayed a good linear range of 0.67 × 10 −6 to 3.33 × 10 −6 mol/L with a lower detection limit at 7.02 × 10 −8 mol/L. The probe FPH were further successfully utilized to detect Ca 2+ in living cells by an increased bright green fluorescence.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）