INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xuanxuan Wang,Huishuang Li,Min Zhou,Guixia Ling,Peng Zhang
Impaired wound healing is one of the major consequences of diabetes, making the development of a more effective treatment for chronic diabetic wounds imperative. Herein, a bilayer hydrogel platform based on chitosan and its derivatives and conjugated with zeolitic imidazole framework-8 (ZIF-8) loaded with glucose oxidase (GOx) was designed, which was used to detect blood glucose content and promote wound healing. Specifically, encapsulation of GOx with ZIF-8 nanoparticles (GZ) were incorporated into the oxidized hyaluronic acid (OHA) and carboxymethyl chitosan (CMCS) hydrogel to construct an inner hydrogel (GZ-OC hydrogel), while the outer hydrogel (CP hydrogel) composed of chitosan/polyvinyl alcohol (CS/PVA) contained 3,3′,5,5′-tetramethylbenzidine (TMB) and horseradish peroxidase (HRP). Leveraging differential swelling and pore sizes between layers, inner-generated H 2 O 2 activated the TMB-HRP system in the outer layer, enabling visual glucose quantification (1–20 mmol·L −1 ) within 20 min. Meanwhile, GZ and natural polysaccharides (CS and CMCS) enabled the bilayer hydrogel to have high antibacterial properties and good biocompatibility, and the bacterial infected wound of diabetic rats basically heals completely within 13 days. This dual-functional platform provided a rapid, safe strategy for personalized diabetic wound management through integrated glucose sensing and antimicrobial regeneration.
研究领域:
生物材料学
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医学工程
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糖尿病研究
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DOI
PubMed
高影响因子
GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2020
·
IF 2.428
Liping Yang,Shaoyang Zhi,Guokun Yang,Chaobin Qin,Wenli Zhao,Mingming Niu,Wenlei Zhang,Wenyu Tang,Xiao Yan,Yuru Zhang,Xiaolin Meng,Ronghua Lu,Guoxing Nie
Irisin, encoded by fibronectin type III domain-containing protein 5 (FNDC5) gene, plays a role in energy expenditure and insulin sensitivity in mice. In fish, the function of irisin related to glucose metabolism is less reported. It may increase glucose utilization in fish. The aim of the present study was to characterize the regulatory role of irisin in glucose metabolism in common carp ( Cyprinus carpio L.). In this study, FNDC5a and FNDC5b were isolated from common carp. The cDNA of FNDC5a and FNDC5b were 722 bp and 714 bp, encoding 221 and 207 amino acids, respectively. FNDC5a was abundantly expressed in the brain and gonad. FNDC5b was mainly expressed in brain. Different expression pattern of FNDC5a and FNDC5b under fasting/refeeding and OGTT experiment were identified. The recombinant common carp irisinA and irisinB were prepared by prokaryotic expression system. Glucose concentration was decreased in treatment with irisinA or irisinB in the in vitro and in vivo experiments. The mRNA expression levels of gluconeogenesis-related genes were significantly down-regulated, while the mRNA expression of glycolysis-related genes were significantly up-regulated after treatment with recombinant irisinA or irisinB in liver in vivo and in primary hepatocytes in vitro. Our research shows that irisin inhibits hepatic gluconeogenesis and promotes hepatic glycolysis. Taken together, this study for the first time revealed the two subtypes of FNDC5 and explored the function and mechanisms of irisinA and irisinB in fish glucose homeostasis.
研究领域:
分子生物学
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内分泌学
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水产养殖
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Sheng Zhang,Xiangzhou Li
We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro . The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose :Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125–0.500 mg/mL. Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration. A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established. This result suggests that CCP can be used as hypoglycemic medicine or health food.
研究领域:
生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhou Chen,Yanyin Song,Yuhan Yan,Wei Chen,Tong Ren,Aijin Ma,Siting Li,Yingmin Jia
A novel cellobiose 2-epimerase gene ( CsCEase1 ) from Clostridium sp. TW13 was successfully overexpressed. Cs CEase1, with a molecular mass of 45.0 kDa, exhibited a specific activity of 254.75 U/mg and showed optimal activity at 40 °C and pH 7.5. Using Cs CEase1, 44.30 % of lactose was converted into epilactose, yielding a production level of 26.58 g/L. An efficient purification method was developed, incorporating crystallization and β-galactosidase treatment to remove the unconverted lactose, while Saccharomyces cerevisiae was used to consume the produced glucose. Final purification with activated carbon resulted in epilactose with 98 % purity and a recovery rate of 66.47 %. Furthermore, Cs CEase1 was incubated with whey power and expired milk, achieving epilactose conversion rates of 29.65 % and 32.69 %, respectively. These findings highlight the potential of Cs CEase1 for the cost-effective and environmentally sustainable production of epilactose from dairy waste.
研究领域:
生物化学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2014
·Vol. 95
·期 11
·2228-2236
·
IF 1.879
Pei-Hong Fan,Mei-Tong Zang,Jie Xing
BACKGROUND As probiotics, soy oligosaccharides have become popular as healthy foods to reduce disease risk. However, comprehensive information about oligosaccharides in different food legumes is limited. RESULTS In this study, eight oligosaccharides were well detected and quantified in different varieties of eight legume species using high-resolution mass spectrometry. It was determined that species could be distinguished by total content of oligosaccharides and their distribution modes. Among the studied species, Vigna unguiculata is a better resource of non-digestible oligosaccharides, while Vicia faba and black soybean ( Glycine max ) are at a disadvantage. Normally, stachyose predominates in non-digestible oligosaccharides, except in mung bean and broad bean, where verbascose predominates. For mung bean and green soybean, the seed coat should be taken into account for oligosaccharide consumption. The developed high-resolution mass spectrometry method greatly simplified the sample preparation process and permitted the identification of oligosaccharides without reference compounds. CONCLUSION This work involved extensive sample collecting and provided useful information for consumers. The developed method may be useful for rapid quantification of oligosaccharides in related foods. © 2014 Society of Chemical Industry
研究领域:
食品科学
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植物化学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Chengjian Wang,Xi Gao,Guiping Gong,Lijuan Man,Qian Wei,Yao Lan,Meifang Yang,Jianli Han,Wanjun Jin,Ming Wei,Linjuan Huang,Zhongfu Wang
Glycomics analysis has been undermined by the lack of structurally defined individual glycans as model compounds. However, it is challenging to prepare individual glycans from natural resources, mainly due to separation difficulties caused by highly diverse structure, complicated mixture form and chromophore-free property of naturally-existing glycans. In this study, we report a simple, universal and low-cost glycan separation strategy, glycoselection, which allows preparation of individual reducing glycans from their mixtures through reversible chromogenic derivatization by hydrazide chemistry in combination with two-dimensional high-performance liquid chromatography (2D-HPLC). Investigations on reaction conditions using lactose and maltodextrin as model glycans showed the feasibility of reversible hydrazide labeling and one-pot hydrazone conversion under mild conditions, the good stability of hydrazone-form derivatives of glycans in solution and the difference among seven selected hydrazine-carrying chromogenic reagents in product yields during glycan labeling and post-column detagging. The 2D-HPLC separation conditions were established on maltodextrin, from which fourteen highly-purified individual reducing oligo-glucans were ultimately obtained. Using this strategy, we also successfully prepared and identified eleven individual neutral reducing N -glycans from chicken ovalbumin and thirteen individual neutral reducing oligosaccharides and eight individual sialylated ones from human milk, demonstrating its good applicability to different types of reducing glycans as well as biological samples. Given the compatibility of individual reducing glycans with almost all of glycan derivatization protocols and analytical techniques of glycans and the potential of the method for larger scale application, this work provides a universal approach to compound-specific analysis of natural glycans and has great significance for glycomics studies.
研究领域:
分析化学
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糖生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 17
·5691
·
IF 4.927
Lan Luo,Xuemei Song,Xiao Chang,Sheng Huang,Yunxi Zhou,Shengmei Yang,Yan Zhu,Lanyan Zhang,Yongsheng Wu,Junyin Zhang,Zhipeng Zhou,Mingyi Wu
Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN), extracted fromMycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an α-D-(1→4)-mannan with (1→2)-linked branches, and an α-D-(1→4)-glucan with (1→6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN.Keywords:active ingredients;polysaccharide purification;glucan;mannan;immunomodulator
研究领域:
免疫学
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药理学
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生物化学
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DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2023
·
IF 3.1
Jianrong Wu,Congsheng Wang,Tiantian Zhang,Hongtao Zhang,Xiaobei Zhan
In this work, a mannan-oligosaccharide conjugate with sialic acid capable of perturbing Aβ42 aggregation was designed and synthesized. Mannan oligosaccharides with degree polymerization of 3–13 were obtained by stepwise hydrolysis of locust bean gum using β-mannanase and α-galactosidase, named as LBOS. The activated LBOS was further chemically conjugated with sialic acid (Sia, N-acetylneuraminic acid) by fluoro-mercapto chemical coupling to synthesize a conjugate LBOS-Sia, and then phosphorylated to obtain pLBOS-Sia. The successful synthesis of pLBOS-Sia was confirmed by infrared1 chromatography, mass spectrometry, and 1 H NMR. The soluble protein analysis, microscopic observation, thioflavin T-labeling, and circular dichroism spectroscopy revealed that both LBOS-Sia and pLBOS-Sia can inhibit Aβ42 aggregation. MTT assay showed that LBOS-Sia and pLBOS-Sia had no cytotoxicity to BV-2 cells, and could substantially reduce the release of pro-inflammatory factor TNF-α induced by Aβ42 in BV-2 cells, and inhibit the occurrence of neuroinflammation. In future, this novel structure of mannan oligosaccharide-sialic acid conjugate can be potentially used to for the development of glycoconjugates against AD targeting Aβ.
研究领域:
生物化学
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神经科学
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药物化学
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DOI
PubMed
高影响因子
AMB Express
·
2019
·Vol. 9
·期 1
·1-11
·
IF 2.226
Guo Zongren,Long Liangkun,Ding Shaojun
d -Mannose and l -ribose are two important monosaccharides, which have attracted public attention recently because of their great application potentials in food, cosmetic and pharmaceutical industries. Sugar isomerases catalyze the sugar isomerization and therefore can be used as the biocatalysts for production of the high-value sugars from inexpensive sugars. l -arabinose isomerase catalyzes the conversion of l -arabinose to l -ribulose, while d -lyxose isomerase catalyzes l -ribulose and d -fructose to l -ribose and d -mannose, respectively. In this paper, a putative d -LI from Bacillus velezensis (BvLI) was identified, characterized and used to produce d -mannose and l -ribose from d -fructose and l -arabinose, respectively. The recombinant BvLI exhibited a maximum activity at 55 °C and pH 6.5, in the presence of 0.1 mM Co 2+ . Approximately 110.75 g/L d -mannose was obtained from 500 g/L d -fructose in 6 h by the recombinant BvLI, and approximately 105 g/L l -ribose was obtained from 500 g/L l -arabinose in 8 h by the successive biocatalysis of l -arabinose isomerase from Bacillus licheniformis (BlAI) and BvLI.
研究领域:
生物化学
·
生物技术
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食品科学
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制药学
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产品关联
DOI
PubMed
高影响因子
PURE AND APPLIED CHEMISTRY
·
2017
·Vol. 89
·期 7
·921-929
·
IF 2.626
Zhi Peng Cai,Wen Li Wang,Louis Conway,Kun Huang,Faisal Nureldin Awad,Li Liu,Josef Voglmeir
We herein demonstrate the use of 1,3-di(2-dipyridyl)propan-1,3-dione (DPPD) as a fluorogenic label for oligosaccharides. A number of milk-derived oligosaccharide standards were successfully labeled with this reagent, with the advantage of greatly simplified sample preparation compared to other commonly used fluorescent tags. DPPD shows a selectivity for oligosaccharides which do not possess a 2-acetamido-2-deoxy-hexose moiety at the reducing terminus, potentially aiding in the identification of complex mixtures of carbohydrates. The use of DPPD for the structural determination of oligosaccharides through exoglycosidase treatment, quantitative analysis of reactions, and in the synthesis of labeled oligosaccharides was also explored. This reagent has, in addition to the analysis of individual and mixed oligosaccharides, potential applications in the study of glycosidases and glycosyltransferases and as such represents a valuable addition to the tools available to the glycoscientist.
研究领域:
糖生物学
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分析化学
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生物化学
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产品关联
DOI
高影响因子
Fishes
·
2025
·Vol. 10
·期 2
·65
·
IF 2.4
Ying Liu,Zongcai Tu,Qiuyu Lu,Shengnan Zhan,Ru Jia,Zhaohui Qiao,Huamao Wei,Tao Huang
The antifreeze and antioxidant capacities of tilapia (Oreochromis mossambicus) gelatin hydrolysates were investigated, after glycosylation with saccharides of varying molecular weights, to enhance their functional properties to widen its commercial application in frozen aquatic products. Glycosylation was conducted by mixing gelatin hydrolysates with ribose, glucose, maltose, and dextran (20 kDa) at a 1:1 mass ratio; the glycosylation products had a pH of 10 and were incubated at 80 °C for 1 h. The results showed that the glycosylation degree ranked as: ribose > glucose > maltose > dextran. The mass spectrometry analysis showed that 17, 32, and 5 glycosylation sites were identified for ribose, glucose, and maltose, respectively, suggesting a molecular weight-dependent effect. Spectroscopic analyses, including ultraviolet and infrared spectroscopy, revealed that the gelatin hydrolysate structure was expanded, with chromophores in hydrophilic environments; a blue shift in the amide A and II bands confirmed that the amino group was involved. Fluorescence spectroscopy showed conformational changes with a red shift at 303.4 nm and a reduction in intensity. Antifreeze activity, such as catalase freezing protection and shrimp surimi protein stability, and antioxidant activity, including radical scavenging and metal ion chelation, were significantly improved. Ribose exhibited the strongest effects, followed by maltose and glucose. These results demonstrate the potential of glycosylation to improve gelatin hydrolysates for functional applications.
研究领域:
食品科学
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生物化学
·
水产养殖
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产品关联
DOI
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Jia-Bin Wang,Le-Yi Yu,Xu Zeng,Jun-Wei Zheng,Bin Wang,Li Pan
Inhibition of α-glucosidase activity is an important strategy in lowering the concentration of blood sugar . In this paper, using domestic and foreign characteristic food-derived substances as the sources of lactobacillus , the performance of them were evaluated by measuring the strains’ α-glucosidase inhibitory ability. Finally, the cell-free extracellular supernatants (CFS) of Lactobacillus rhamnosus LB1lac10 was determined to have the highest α-glucosidase inhibition ability. Based on the Nanopore third-generation sequencing technology platform, the genome of LB1lac10 was sequenced and functional gene annotation was performed. After that, the biological activity and structural composition of the exopolysaccharide produced by L. rhamnosus LB1lac10 were studied. The purified exopolysaccharide EPS1-1 also showed efficient α-glucosidase inhibitory ability. The structure and conformation characteristics of EPS1-1 were further analyzed. The EPS1-1 from L. rhamnosus LB1lac10 had a molecular weight of 88,650 Da, and it was mainly composed of mannose , glucuronic acid , glucose, xylose , galactose , and arabinose . From the FT-IR and NMR analyses, EPS1-1 had functional groups of a typical polysaccharide structure and contained two types of glycosidic bonds with α-configuration pyranose . The main glycosidic bond corresponded to → 4)-α- d -Glcp-(1→, which might be an important reason why EPS1-1 could inhibit α-glycosidase. Thermodynamic studies showed that EPS1-1 had high heat resistance to meet the needs of food processing. The results suggested that L. rhamnosus LB1lac10 could be used as a potential probiotic to lower blood sugar, and the EPS1-1 has the potential to serve as a natural α-glycosidase inhibitor to regulate the concentration of blood glucose.
研究领域:
微生物学
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分子生物学
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生物化学
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食品科学
·
药理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）