MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Wang,Yawen Liu,Wenping Liu,Huabing Yang,Haiming Hu,Zhigang Zhang,Songlin Liu,Junping Zheng,Hongtao Liu
Plasmonic nanosensors have garnered increasing attention due to their naked-eye visibility and facile preparation. However, the inadequate stability and specificity significantly challenged their widespread application. Here, we hypothesized that capping with double-charged acids and loading in the hydrogel should bring gold nanoparticles (AuNPs) more stability and specificity for sensing small molecules. In the plasmonic nanosensor, aspartic acid-capped AuNPs (Asp-AuNPs) served as both the sensing material and colorant. Compared with conventional AuNPs, Asp-AuNPs exhibited a lower Zeta potential and enhanced selectivity for histamine. Moreover, Asp-AuNPs rapidly responded to histamine in less than 2 min, even after seven weeks of storage. The isothermal titration calorimetry analysis further supported the affinity of Asp-AuNPs for histamine. Using the alginate hydrogels as a matrix for Asp-AuNPs, the hydrogel-based plasmonic nanosensor could detect histamine on the surface of and in the atmosphere around expired fish. In summary, we have developed a novel, durable, and stable hydrogel-based plasmonic nanosensor for contact and contactless freshness detection.
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JOURNAL OF PROTEOME RESEARCH
·
2024
·
IF 3.8
Jie Zhang,Xiaoling Zang,Peng Jiao,Jiangyu Wu,Wei Meng,Lizhen Zhao,Zhihua Lv
Abnormal lipid metabolism plays an important role in cancer development. In this study, nontargeted lipidomic study on 230 tissue specimens from 79 nonsmall cell lung cancer (NSCLC) patients was conducted using ultraperformance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS). Downregulation of sphingosine and medium–long-chain ceramides and short–medium-chain acylcarnitine, upregulation of long-chain acylcarnitine C20:0, and enhanced histamine methylation were revealed in NSCLC tissues. Compared with paired noncancerous tissues, adenocarcinoma (AC) tissues had significantly decreased levels of sphingosine, medium–long-chain ceramides (Cer d18:1/12:0 and Cer d16:1/14:0, Cer d18:0/16:0, Cer d18:1/16:0, Cer d18:2/16:0, Cer d18:2/18:0), short–medium-chain (C2–C16) acylcarnitines, LPC 20:0 and LPC 22:1, and significantly increased levels of the long-chain acylcarnitine C20:0, LPC 16:0, LPC P-16:0, LPC 20:1, LPC 20:2, glyceroPC, LPE 16:0, and LPE 18:2. In squamous cell carcinoma (SCC) tissues, sphingosine, Cer d18:2/16:0 and Cer d18:2/18:0, and short–medium-chain acylcarnitines had significantly lower levels, while long-chain acylcarnitines (C20:0, and C22:0 or C22:0 M), LPC 20:1, LPC 20:2, and N1,N12-diacetylspermine had significantly higher levels compared to controls. In AC and SCC tissues, the levels of LPG 18:0, LPG 18:1, and LPS 18:1 were significantly decreased, while the levels of ceramide-1-phosphate (C1P) d18:0/3:0 or LPE P-16:0, N1-acetylspermidine, and 1-methylhistamine were significantly increased than controls. Furthermore, an orthogonal partial least-squares-discriminant analysis (OPLS-DA) model based on a 4-lipid panel was established, showing good discrimination ability between cancerous and noncancerous tissues.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Di Jiao,Zhen-Jie Qian,Yu Wang,Xi Zeng,Yu-Xin Zhong,Lin Luo,Yong-Xin He,Yi-Na Lu,Hong-Tao Lei,Zhen-Lin Xu
Gizzerosine is a biogenic amine in feeds with a physiological toxicity 1,000 times that of its precursor, histamine, causing high mortality in poultry. Because feeds containing gizzerosine usually contain high concentrations of histamine, it is of great significance to establish a method for the simultaneous detection of gizzerosine and histamine for feed safety. Herein, we first developed a detection method using 50 % (v/v) acetonitrile(ACN) containing 1 % (v/v) ammonium hydroxide (AH) extraction to synchronously monitor gizzerosine and histamine in feeds and raw materials via UHPLC–MS/MS. Due to the serious positive matrix effect (ME) in existing LC–MS/MS, this study reduced the ME of samples by purifying the extract solution using a mixed-mode cation exchange based solid phase extraction column (MCX-SPE), and simultaneously obtained accurate quantities of 5–500 ng mL −1 gizzerosine and 25–2,500 ng mL −1 histamine. The limit of detection (LOD) values for gizzerosine and histamine were 1.02 ng mL −1 and 3.00 ng mL −1 , respectively, which were approximately 390 and 8,000 times more sensitive than the maximum recommended dosage of gizzerosine (0.4 mg kg −1 ) and limitation standards of histamine (25 mg kg −1 ) in fish meal, respectively. The samples could only be evaluated in 2 h. The average recoveries of the 6 types of feeds and raw materials ranged from 79.73 % to 110.31 % with a relative standard deviation (RSD) ranging from 1.29 % to 13.35 %. The average recoveries of feeds were generally lower than those of raw materials. Our method was efficient, simple, rapid, accurate, sensitive, and suitable for the simultaneous quantitative analysis of gizzerosine and histamine contents in various feeds, which provided technical support for the quality evaluation of feeds.
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TALANTA
·
2024
·
IF 5.6
Jiahui Zou,Guancheng Zhu,Xueer Lin,Lanling Chu,Huimin Zhong,Cong Jiang,Yanyan Huang
Biogenic amines (BAs) are hazardous substances found in fishery products that are closely associated with fish spoilage and threaten food safety. Traditional chromatographic methods for detecting BAs are expensive, complex, and time-consuming. In this study, we developed a nanozyme-based sensor array to efficiently discriminate between four types of BAs and sensitively detect histamine. Copper-, cerium-, and manganese-based metal-organic frameworks with excellent peroxidase-like activities were employed as sensor elements. Because the catalytic activities of metal-organic frameworks could be modified by different BAs to varying degrees, the sensor array could generate a distinct colorimetric response pattern (fingerprint) for each BA. Based on this principle, the sensor array accurately discriminated BAs over a wide concentration range (10–1000 μM). Histamine could be distinguished down to 1 μM and detected with a detection limit of 4.28 μM within 20 min. In addition, mixtures of BAs, target BAs, interfering substances, and BAs in fishery product samples were well discriminated. Furthermore, our sensor array could also effectively distinguish the freshness of fish samples. This work might offer a useful strategy for the discrimination and detection of BAs and could positively contribute to food safety and public health.
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TALANTA
·
2024
·
IF 5.6
Yan Su,Pengfei Gao,Guomei Zhang,Ying Zhou,Lihong Shi,Jianlin Wu,Wenting Liang,Shaomin Shuang,Yan Zhang
In this study, the N-Acetyl- l -Cysteine (NAC)-capped gold and silver bimetallic nanoclusters (NAC@Au–Ag NCs) was synthesized by reflux method. Due to the silver effect, the NAC@Au–Ag NCs exhibited strong photoluminescence at far-red/near-infrared regions and better catalytic performance than Au or Ag NCs. Upon addition of H 2 S, the Au–Ag NCs exhibited obvious fluorescence quench and color changes through the generation of metal sulfides and a static quenching process. The Au–Ag NCs displayed a wide linear luminescence response for H 2 S (18.5–217 μmol/L) with a detection limit of 0.269 μmol/L. Moreover, the visible color of Au–Ag NCs changed from white to brown-yellow along with increased H 2 S, the corresponding RGB values also displayed good linearity with the concentration of H 2 S (90.1–678 μmol/L). Notably, the fabrication of test strips provided a convenient and intuitive tool to screen the freshness of eggs by the color change of test strips. Au–Ag NCs could be used for living HeLa cells bioimaging and recognition of H 2 S abnormalities. Furthermore, it can be used as a catalyst to reduction of nitrophenols (NPhs), specific included 2NP, 3NP and 4NP. The reaction was regarded as a pseudo-first-order kinetic reaction due to the presence of excess NaBH 4 . At 298K, the catalytic rate constants(k) of 2NP, 3NP and 4NP were 0.1754 min −1 , 0.1734 min −1 and 0.2782 min −1 , respectively. The NAC@Au–Ag NCs catalyst still showed good catalytic activity and reusability after five cycles. Therefore, this study developed a H 2 S sensor for food samples and biological systems. And this nanocatalyst had great application potential for removed the nitrophenol pollutants in water.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Nian Shi,Wenxuan Li,Yuwei Liu,Shumei Yan,Xia Xu,Di Chen
The determination of biogenic amines (BAs) in alcoholic beverages is crucial for assessing their health impact, ensuring beverage quality, and guaranteeing safety. Herein, a rapid one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method was proposed using 6-aminoquinolinyl-N-hydroxysuccinimide carbamate as the derivatization reagent and magnetic hydroxyl-functionalized multi-walled carbon nanotubes as the extraction material. Integration of derivatization and extraction steps simplifies the sample preparation process, taking only three minutes and eliminating the need for centrifugation by utilizing magnetic sorbent. The resulting desorption solution was directly analyzed by high-performance liquid chromatography-fluorescence detection (HPLC-FLD) without any evaporation or reconstitution steps. The integrated OPD/MSPE-HPLC-FLD method demonstrates excellent linearity (R 2 > 0.992), accuracy (relative recoveries: 85.1–109.2%), precision (RSDs≤9.7%) and detection limits (limits of detection: 0.3–2 ng/mL). It has been successfully applied to determine free BAs in various alcoholic beverages, including red wine, Baijiu, Huangjiu, and beer. This method enables rapid, sensitive and precise analysis of BAs in alcoholic beverages.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-19
·
IF 14.7
Li Bo,Chen Gengjia,Zhong Huihai,Li Tan,Lin Minzhao,Wei Huiye,Zhang Qiaoyun,Chen Qi,Huang Jinsheng,Shuai Xintao
Effective treatment against glioma remains challenging nowadays because the protective blood-brain barrier (BBB) impedes drug penetration into brain and the limited efficacy of conventional chemotherapy. While strong positively charged nanoparticles have good permeability through the BBB, they often come with the caveat of cationic toxicity to healthy tissues and organs during blood circulation. Here we show a neutrally charged nanoprobe with a surface decorated with γ -glutamyl moieties that can be cleaved by γ -glutamyl transpeptidase, an enzyme overexpressed on brain capillaries. Upon the cleavage, positively charged primary amines are generated, facilitating the effective crossing of the nanoprobe through BBB via the adsorption-mediated transcytosis pathway, while avoiding the caveat of cationic toxicity. In addition, when reaching the acidic tumor microenvironment, the nanoprobe co-encapsulating sonosensitizer and immune agonist swells, which results in an accelerated drug release under ultrasound irradiation to induce a combined immune response, ultimately leading to a robust anticancer effect. Overall, we report an effective drug delivery nanoplatform across the BBB for an enhanced therapy of glioma.
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SENSORS
·
2024
·Vol. 24
·期 23
·7415
·
IF 3.4
Haichao Ye,Liqin Liu,Dagang Shen,Chang Song,Huanhuan Wang
BF3, volatile amines (VOAs), and biogenic amines (BAs) are the key indicators in chemical reaction catalysis and food quality monitoring. In this study, we present two types of fluorescent sensors, a hydrazone ligand (HL)-based fluorescent sensor for BF3detection and a novel sensor array using six boron difluoride (BF2) hydrazone complexes (BFHs) for monitoring VOAs and BAs. Spectral research indicates that the interaction mechanism between the HLs and BF3is based on intramolecular charge transfer (ICT). The HLs for the monitoring of BF3showed good sensitivity, selectivity, and anti-interference and have the characteristics of a visible color change. Additionally, the HL probe demonstrates reversibility in the presence of triethylamine, making it a candidate for “ON-OFF-ON” mode sensing. BF3detection can also be efficiently performed using test strips for convenient, air-based applications. The BFH sensor array successfully differentiates histamine from the other typical non-volatile BAs in solution; in comparison, the VOAs are analyzed through recognition patterns and statistical analysis. The array’s color changes enable the practical, on-site detection of shrimp spoilage, with principal component analysis distinguishing various ageing intervals. In summary, this sensor array demonstrates high selectivity for VOAs and BAs, with significant potential for application in real-world sample analysis.
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FOOD CONTROL
·
2024
·
IF 5.6
Zhen Wu,Yi Wei,Yue Tang,Lian Xia,Xiaojuan Niu,Yuangen Wu
Food poisoning caused by excessive intake of histamine is a significant public health concern. Traditional analytical methods struggle to meet the demands for on-site monitoring of histamine in food. Colorimetry based on nanozyme catalysis can be applied to rapidly detect histamine, but some challenges remain in the specific response of nanozymes to histamine. Herein, a novel “off-on” colorimetric sensor was first established for histamine detection based on Cu 2+ -mediated laccase-mimic activity of flower-like Mn 3 O 4 nanoparticles ( Mnf ). This work proved that Cu 2+ can inhibit the laccase-mimic activity by hindering the electron transfer between Mnf and 2,4-DP molecules. Once histamine was present in the system, Cu 2+ was first consumed due to the coordination with histamine, resulting in the recovery of laccase-mimic activity of Mnf . The color of sensing solution can be converted to the corresponding RGB value by a smartphone for on-site detection of histamine in food. The colorimetric sensor has a limit of detection as low as 0.148 μg mL −1 , which is comparable to that of most colorimetric methods. Moreover, the colorimetric sensor exhibits high selectivity, and has considerable applicability for histamine detection in aquatic products.
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Food Bioscience
·
2025
·
IF 5.9
Hongwei Zhan,Xiang Zou,Jiao Li,Lixiu Zhang,Guanyuan Qu,Yanqing Li,Yongjun Sun,Wenming Ju,Mengqi Ye,Yang Deng,Ting Ding
Pseudomonas fluorescens is a prevalent spoilage bacterium in various chilled aquatic products, producing toxic biogenic amines (BAs) through the decarboxylation of amino acids via amino acid decarboxylases. Our previous studies revealed that P. fluorescens PF12 regulates BA production through its quorum sensing (QS) system, suggesting that disrupting QS could be a potential approach to control BA generation. In this study, we targeted the three-dimensional structure of the LuxI-type protein in P. fluorescens PF12 to screen for QS inhibitors (QSIs) through high-throughput screening from Seaweed Metabolite Database and Comprehensive Marine Natural Products Database. Nine compounds with high binding scores to the LuxI-type protein were identified. Considering cost-effectiveness and safety, phytol was selected for further investigation. Experimental results demonstrated that sub-inhibitory concentrations of phytol significantly suppressed BA production, siderophore production, motility, extracellular protease secretion, and biofilm formation in P. fluorescens PF12. Specifically, when the salmon fillets were stored for 288 h, the histamine content in the fillets treated with 25 mg/mL phytol decreased by 43.81 %. RT-qPCR revealed that the expression levels of key genes hisD and hisC related to histamine synthesis in the phytol treatment group were reduced by 7.68 ( p < 0.05) and 8.23 % ( p < 0.05), respectively, compared to the control group. Molecular docking analysis revealed that phytol interacts with key residues in the LuxI-type protein, thereby blocking the synthesis of acyl-homoserine lactones (AHLs) and downstream QS signaling. These findings indicate that phytol has great potential for controlling BA production in aquatic products and ensuring food safety.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Lin Deng,Zhihui Xia,Ting Peng,Zhou Shi,Haojie Zhang
Sulfadiazine (SDZ), a widely used antibiotic, poses significant environmental risks due to its persistence and potential to induce bacterial resistance. This study presents a novel FeOOH-modified iron foam (FeOOH/IF) catalyst for efficient peroxymonosulfate (PMS) activation to degrade SDZ. The catalyst was synthesized via a simple NaBH₄-induced surface modification, forming FeOOH nanoflakes with abundant oxygen vacancies. Comprehensive characterization (SEM, TEM, XRD, XPS, EPR) confirmed the successful formation of FeOOH/IF with enhanced catalytic properties. The FeOOH/IF/PMS system achieved nearly complete SDZ removal (100 %) within 20 min, exhibiting 4.7 times higher k obs value (0.18 min −1 ) than unmodified IF (0.038 min −1 ). Mechanistic studies revealed a dual radicals ( • OH and SO 4 • − ) and non-radicals ( 1 O 2 , Fe IV O, electron transfer) oxidation pathway, with non-radical processes dominating (53.12 % contribution). DFT calculations and LC-MS identified four primary degradation pathways, demonstrating effective detoxification of SDZ. Moreover, the as-synthesized exhibited excellent recyclability (over 90 % removal in the four repeated cycles) and versatility, efficiently degrading various organic pollutants (tetracycline, carbamazepine, etc.). This work provides a highly efficient, stable, and eco-friendly PMS-activating catalyst for antibiotic wastewater treatment, with insights into reaction mechanisms and practical applicability.
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PHYTOMEDICINE
·
2025
·
IF 8.3
Xue-Ting Xu,Jun-Kai Fan,Xi Chen,Si-Yi Que,Run-Xin Zhang,Yu-Yan Xie,Tian-Ci Zhou,Kunmei Ji,Zhen-Fu Zhao,Jia-Jie Chen
Background Mast cells (MCs) mediate high-affinity IgE Fc receptor (FcεRI)-mediated allergic reactions. Royal jelly acid (RJA), the major lipid constituent of royal jelly, has anti-inflammatory, anti-tumor, antibacterial, and immunomodulatory properties. Purpose This study aimed to investigate how RJA affects IgE-mediated MC activation and to elucidate mechanisms underlying anti-allergic effects. Methods Mouse bone marrow-derived mast cells (BMMCs) and rat basophilic leukemia cells-2H3 (RBLs) were stimulated with IgE-antigen (Ag). β-hexosaminidase (β-hex) and histamine (HA) release, inflammatory cytokines expression, and cellular morphology changes were examined in stimulated cells. Transcriptome changes associated with RJA-treated activated-MCs were analyzed. Network pharmacology, gene expression, and western blot analyses were employed to explore the relationship between H3 lactylation and anti-allergic effects of RJA on MC. In vivo studies were conducted in IgE-mediated passive cutaneous anaphylaxis (PCA), ovalbumin (OVA)-induced active systemic anaphylaxis (ASA), and HA-induced passive systemic anaphylaxis (PSA) mouse models. Results RJA inhibited IgE-dependent MC activation as evidenced by reduced β-hex and HA release. RJA inhibited MAPK and NF-κB signaling, down-regulated RELA and NFκB1 transcripts, and decreased NF-κB reporter activity. RJA inhibited cellular H3 lactylation and H3K9 lactylation (H3K9la), leading to reduced histone lactyllysine enrichment in RELA or NFκB1 promoter loci. RJA alleviated allergic symptoms in PCA and ASA mice by intraperitoneal injection or oral administration, but did not affect HA-induced hypothermia. Conclusion RJA inhibits IgE-dependent MC activation by reducing cellular H3K9la and suppressing NF-κB signaling via reduced histone lactyllysine enrichment in RELA or NFκB1 promoter sites. RJA may support MC inhibition in the prevention and treatment of allergic diseases.
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