Food Quality and Safety
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2022
·
IF 3.949
Song Jing,Zhang Huaibi,Wang Zhenxing,Wang Juan
ObjectivesThis study aimed to evaluate the functional activity and phytochemical composition in the flower petals of Paeonia delavayi (P. delavayi) in different colors.Materials and MethodsP. delavayi petal extracts were prepared by maceration in methanol, including purple petal extract (PPE), red petal extract (RPE), and yellow petal extract (YPE), and their antioxidant activity and α-glucosidase and acetylcholinesterase inhibition activities were evaluated. To correlate these measured activities to phytochemicals in the petals, an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)-based metabolomics method was applied to profile the compositions in the petals of different colors. Finally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways database was used to identify the related metabolic pathways that are responsible for the production of these polyphenolic phytochemicals in the petals.ResultsThe results showed that PPE had the highest total phenolic content, total flavonoid content, and the strongest 2,2ʹ-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging ability, ferric reducing antioxidant power, and acetylcholinesterase inhibition ability in all three samples, while YPE showed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and α-glucosidase inhibition ability. A total of 232 metabolites were detected in the metabolomic analysis, 198 of which were flavonoids, chalcones, flavonols, and anthocyanins. Correlation analysis indicated that peonidin-3-O-arabinoside and cyanidin-3-O-arabinoside were the major contributors to the antioxidant activity. Principal component analysis showed a clear separation among these three petals. In addition, a total of 38, 98, and 96 differential metabolites were identified in PPE, RPE, and YPE, respectively. Pathway enrichment revealed 6 KEGG pathways that displayed significant enrichment differences, of which the anthocyanin biosynthesis, flavone and flavonol biosynthesis were the most enriched signaling pathways, revealing a potential reason for the differences in metabolic and functional levels among different colors of P. delavayi petal.ConclusionsP. delavayi petals in different colors have different metabolite contents and functional activities, of which the anthocyanin, flavone, and flavonol metabolites are critical in its functional activities, suggesting the anthocyanin biosynthesis, flavone and flavonol biosynthesis pathways are the key pathways responsible for both petal color and bioactive phytochemicals in P. delavayi flowers.
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Frontiers in Chemistry
·
2022
·
IF 5.545
Kuang Yi,Xia Yunlong,Wang Xing,Rao Qingqing,Yang Shengxiang
4-hydroxyl coumarin (HC), an important intermediate during the synthesis procedure of rodenticide and anti-cardiovascular drug, shows highly medicinal value and economic value. To achieve the efficient adsorption of HC from natural biological samples, a novel magnetic surface molecularly imprinted polymer (HC/SMIPs) was constructed by employing methacrylic acid (MAA) as functional monomer, organic silane modified magnetic particles as matrix carrier and HC as template molecule. Due to the numerous specific imprinted cavities on the HC/SMIPs, the maximum adsorption capacity of HC/SMIPs for 4-hydroxycoumarin could reach to 22.78 mg g−1 within 20 min. In addition, HC/SMIPs exhibited highly selective adsorption for 4-hydroxycoumarin compared with other active drug molecules (osthole and rutin) and showed excellent regeneration performance. After 8 cycles of adsorption-desorption tests, the adsorption capacity of HC/SMIPs just slightly decreased by 6.64%. The efficient selective removal and easy recycle of 4-hydroxycoumarin from biological samples by HC/SMIPs made a highly promising to advance the application of imprinting polymers in complex practical environments.
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Food and Bioprocess Technology
·
2022
·Vol. 15
·期 5
·1101-1114
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IF 5.581
Javed Miral,Belwal Tarun,Ruyuan Zhang,Xu Yanqun,Li Li,Luo Zisheng
Camellia oleifera shells are a noteworthy by-product often considered as agricultural waste and discarded rather than efficiently used. The extraction of bioactive compounds from shells using green extraction process can be a better approach for waste valorization. Also, demand for novel and green extraction method is required to avoid organic solvents, high cost, and environmental problems. Nanobubbles (NBs) can be used for extraction purposes due to their high specific area and internal pressure. In this study, a novel method using nanobubbles (NBs) solution coupled with ultrasonication to extract phytochemicals from Camellia Oleifera shells (COSs) was first explored. The characterization was performed to measure the size, zeta potential, and surface tension of NBs stabilized by biosurfactant in this case rhamnolipid (Rh). The produced NBs were of size 100 nm, having a surface tension of 35.26 ± 0.31 mN/m −1 . Single-factor experiments were performed to determine the significant variables. The Box–Behnken design optimized the ultrasonic power, ultrasonic time, and surfactant concentration to maximize the phytochemicals extraction. NBs solution coupled with ultrasonication (US) leads to a higher extraction yield of total phenolic (34.42 mg of gallic acid equivalent per gram of dry weight (mg GAE/g DW)) and flavonoid content (23.36 mg of rutin equivalent per gram of dry weight (RE/g DW)), as compared to the conventional extraction method. In addition, in vitro antioxidant activity was also increased in NBs solution and NBs’ solution–US extract compared with the conventional solvent method. The morphological and microstructural changes under NBs solution and ultrasonication were also determined to provide a possible extraction mechanism. The present experiment provides a novel extraction method and an overall green perspective of agro and food waste valorization.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 8
·3453-3467
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IF 3.094
Ye Hangyu,Li Xiaojun,Li Luyuan,Zhang Yinjun,Zheng Jianyong
Aspergillus niger has been used for homologous and heterologous expressions of many protein products. In this study, the α-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was homologously expressed in A. niger 3.350 by Agrobacterium tumefaciens -mediated transformation. The enzyme activity of Rha-N1 was 0.658 U/mL, which was obtained by cultivation of engineered A. niger in a 5-L bioreactor. Rha-N1 was purified by affinity chromatography and characterized. The optimum temperature and optimum pH for Rha-N1 were 60 °C and 4.5, respectively. Enzyme activity was promoted by Al 3+ , Li + , Mg 2+ , and Ba 2+ and was inhibited by Mn 2+ , Fe 3+ , Ca 2+ , Cu 2+ , and organic solvents. The result indicated that rutin was the most suitable substrate for Rha-N1 by comparison with the other two flavonoid substrates hesperidin and naringin. The transformed products of isoquercitrin, hesperetin-7-O-glucoside, and prunin were identified by LC–MS and 1 H-NMR.
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FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Shengtao Bo,Sui Kiat Chang,Youxia Shan,Yipeng Chen,Hui Liu,Bailin Li,Yueming Jiang,Hong Zhu,Bao Yang
Dietary prenylated stilbenoids present in many food resources, and have good bioactivities. However, their structure–activity relationships are rarely reported. In this work, eighteen C- and O-prenylated stilbenoids were chemically semisynthesized using one-step approach. They all could inhibit sugar digestive enzymes, including α-glucosidase and α-amylase. 4-Geranyl piceatannol from jackfruit showed the strongest activity by suppressing dipeptidyl peptidase-IV (DPP-IV) activity. The enzyme inhibition kinetics were measured and the inhibition mechanism was revealed. Evaluation of antioxidant activity highlighted that the introduction of prenyl with increasing prenyl chain length can significantly increase the antioxidant activity of stilbenoids. Our results suggested that prenylated stilbenoids could be used as functional food additives to decrease postprandial blood sugar levels by inhibiting sugar digestive enzymes and DPP-IV. Prenylated stilbenoids present remarkable DPP-IV inhibitory activity, providing more useful information for the prevention of type 2 diabetes.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Jiaodi Li,Chenxi Wang,Xiaoling Chen,Minghui Huang,Qian Fu,Rongjie Li,Yilin Wang,Chunyan Li,Pengcheng Zhao,Yixi Xie,Junjie Fei
Gallic acid (GA) is found in a wide range of natural plants and is relevant to the health of human beings. Here, a photoelectrochemical sensing platform based on g-C 3 N 4 @CNT heterojunction has been prepared for the highly sensitive and selective detection of GA. Under the light of xenon lamp, the photocurrent of g-C 3 N 4 @CNT is 7 times higher than that of g-C 3 N 4 . And the sensor generates 4 times more photocurrent in the presence of GA than without GA. This sensor has a wide linear range from 10 nM to 10 μM with a limit of detection as low as 2 nM. Also, the abundant amino groups of g-C 3 N 4 provide excellent selectivity for the sensor. Furthermore, the sensor can be used for the analysis of GA in black tea samples, which provides a novel and rapid method for the detection of GA in food samples.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Songjie Li,Weimin Zhang,Ruimin Wang,Congfa Li,Xue Lin,Lu Wang
Partridge leaves ( Mallotus furetianus Muell-Arg.) have long been consumed as popular folk substitute tea for treating hyperglycemia in China. In this study, the inhibiting effects of partridge tea extracts on α -glucosidase and α -amylase were investigated, and then effect of partridge tea aqueous extracts (PTAEs) on glucose consumption capacity of 3 T3-L1 preadipocytes cells was determined. Results verified that PTAEs showed excellent anti- α -glucosidase and anti- α -amylase effects. In addition, the PTAEs evidently promoted glucose consumption capacity of 3T3L1 preadipocytes cells. To this end, a combined method of affinity ultrafiltration and HPLC-ESI-qTOF-MS/MS was used for rapidly screening and identifying the potential inhibitors in the PTAEs. Catechin, epicatechin, rutin, ferulic acid, and kaempferitrin with high affinity capacity indicated strong inhibiting effect on α -glucosidase and α -amylase. Docking studies revealed the potential interactive mechanisms between these major inhibitors and two digestive enzymes. This research shows that partridge tea is effective in preventing and treating post hyperglycemia.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Shengtao Bo,Sui Kiat Chang,Ting Zhou,Hong Zhu,Yueming Jiang,Bao Yang
Prenylated stilbenoids are good candidates of nutraceuticals presented in food resources. The levels of natural prenylated stilbenoids are usually low. Biotransformation is a promising synthesis strategy to produce novel bioactive compounds. However, information regarding biosynthesis of prenylated stilbenoids is rare. In this work, prenyltransferase and geranyl diphosphate biosynthesis pathway were overexpressed in E. coli . Multiple prenyltransferase genes were tested and Ambp1 was found to be effective on resveratrol geranylation. The products were identified by mass spectrometry and nuclear magnetic resonance spectroscopy as 4- C -geranyl resveratrol ( 1 ) and 3- O -geranyl resveratrol ( 2, novel chemical). By optimization of culture conditions, a yield of 36.9% was achieved for the conversion to geranylated resveratrol from resveratrol. These two compounds demonstrated good antioxidant activities with IC 50 values of 28.09 μM for 4- C -geranyl resveratrol and 403.88 μM for 3- O -geranyl resveratrol. The results were helpful for developing novel technique to produce prenylated phenolics.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
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IF 7.129
Danyu Zhao,Huilan Yi,Nan Sang
Arsenic (As) is known to induce toxic responses in many organs of human beings and animals. However, research concerning toxicity in the stomach is limited. In this study, arsenic-induced gastric toxicity was investigated in a mouse model, and grape skin extract (GSE) was confirmed to have protective effects against arsenic toxicity. Our experimental results showed that exposure to 10 mg/l arsenic via drinking water for 56 days caused oxidative damage and inflammatory responses. The H 2 O 2 and malondialdehyde (MDA) contents were significantly increased, accompanied by significant decreases in total superoxide dismutase (T-SOD) activity and glutathione (GSH) content in the gastric tissue of arsenic-treated mice. Two inflammatory signalling pathways, i.e., TLR2/MyD88/NF-κB and IL-6/STAT-3, were activated, along with inflammatory cell infiltration and the elevated mRNA expression of pro-inflammatory cytokines ( TNF-α, IL-1β and IFN-γ ) and myeloperoxidase (MPO) in the gastric tissue of mice exposed to arsenic. Meanwhile, the mRNA levels of the ZO-1 , ZO-2 and occludin genes, which encode the key components of tight junction (TJ) complexes, were downregulated. However, the application of GSE (300 mg/kg bw) significantly inhibited the arsenic-induced increases in H 2 O 2 and MDA contents and the decreases in T-SOD activity and GSH content. The arsenic-mediated gene expression of pro-inflammatory cytokines (TNF-α, IL-1β and IFN-γ), MPO and IL-6/STAT3 and TLR2/MyD88/NF-κB pathways was found down-regulated. Moreover, the arsenic-induced inflammatory cell infiltration and inhibition of TJ genes transcription were markedly attenuated in the As+GSE (300 mg/kg bw) group. Based on the present findings, arsenic intake appears to cause gastric toxicity via oxidative stress and inflammation, and the application of GSE offers significant protection against arsenic toxicity in a mouse model by attenuating the oxidative stress and inflammatory response. Our results suggest that GSE by oral administration might function as a candidate therapeutic supplement to antagonize arsenic toxicity.
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JOURNAL OF FOOD SCIENCE
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2022
·Vol. 87
·期 2
·651-663
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IF 3.693
Hao-Xiang Gao,Heng-Yu Liang,Nan Chen,Bi Shi,Wei-Cai Zeng
Ligustrum robustum (Rxob.) Blume is traditionally served as a functional tea in China. In this work, the antioxidant activities of L. robustum (Rxob.) Blume extract (LRE) were evaluated and its inhibitory effect and mechanism on pancreatic lipase were further investigated. With the high contents of phenols (139.70 ± 1.41 mg gallic acid equivalent/g extract) and flavonoids (326.46 ± 7.36 mg rutin equivalent/g extract), LRE showed significant antioxidant activities ( p < 0.05) for scavenging free radicals and hydrogen peroxide, inhibiting lipid peroxidation and providing strong reducing power. Meanwhile, LRE displayed remarkable inhibitory activity on pancreatic lipase with a low half-effective inhibitory concentration (IC 50 ) of 2.469 ± 0.005 mg/ml which was further determined as non-competitive inhibition. The spectroscopic results showed that LRE inhibited the activity of pancreatic lipase by modifying the tertiary and secondary structures of lipase. Moreover, four phenolic compounds (acteoside, lipedoside A, oleuropein and ligurobustoside C) were identified from LRE by the high performance liquid chromatography-quadrupole- time of flight-mass spectrometry. In addition, according to molecular docking analysis, the four phenols could interact with pancreatic lipase by hydrogen bonds, so as to change the spatial structure of pancreatic lipase and inhibit its catalytic activity. The present results suggest that LRE not only exhibits strong antioxidant capacity but possesses effectively inhibitory activity on pancreatic lipase, which might have the potential to be developed as functional food and nutraceuticals for the prevention of metabolic diseases. Practical Application Ligustrum robustum (Rxob.) Blume extract has been confirmed to possess antioxidant activity and lipase inhibitory activity, which indicates that the L. robustum extract has the potential to prevent oxidative stress and regulate fat metabolism. This work suggests that L. robustum extract can be served as a novel resource to prepare nutraceuticals and functional food in food industries.
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JOURNAL OF FOOD SCIENCE
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2022
·Vol. 87
·期 3
·1230-1243
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IF 3.693
Qi-rui Hu,Peng-wei Lai,Fang Chen,Yan-fang Yu,Bing Zhang,Hongyan Li,Rong Liu,Yawei Fan,Ze-yuan Deng
This study investigated the potential of processing whole mulberry leaves as nutraceutical foods rich in phenolic compounds by spray drying with different drying aids. The results indicated that the spray-dried product 6 (24.2% whey protein isolate [WPI], 33.3% mulberry leaves solid, 38.7% maltodextrin, 3.8% soybean lecithin) with high WPI/mulberry leaves solid ratio possessed the best physical properties, the highest total phenolic compounds level and antioxidant capacity among all the products. Specifically, free chlorogenic acid and rutin were increased by two to three times, but free isoquercitrin and astragalin lost more than 50% in product 6 compared with fresh mulberry leaves. For in vitro digestion, rutin, isoquercitrin, and astragalin (the antioxidative phenolic compounds in mulberry leaves) showed higher bioaccessibility than chlorogenic acid ( p < 0.05) in product 6. Meanwhile, the phenolic compounds bioaccessibility of product 6 was 10–20 times higher than that of fresh whole mulberry leaves. Considering the increased level and bioaccessibility of phenolic compounds, whole mulberry leaves could be developed as potential functional foods by spray drying under the protection of WPI. Practical Application The spray-dried whole mulberry leaves can be consumed as a beverage, meal replacement powder, or used as additive during food processing.
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Journal of Environmental Sciences
·
2022
·
IF 6.796
Yunhao Zhang,Yi Qian,Ming Zhang,Weichuan Qiao
The wastewater discharge from the process of chrome plating, which contains 6:2 chlorinated polyfluorinated ether sulfonate (F-53B) and chromium (Cr), may be toxic to biofilm. In this study we found that the biofilm formed by Pseudomonas aeruginosa PAO1 was inhibited by exposure to a combination of F-53B and Cr(VI). The combined pollution damaged the cell membranes and the structure of the biofilm, and inhibited the production of the Pseudomonas quinolone-based signal, which affected biofilm formation. Moreover, the secretion of extracellular polymeric substances decreased as a result of this combined exposure. Exposure to F-53B and Cr(VI) individually or in combination could induce the excessive accumulation of intracellular reactive oxygen species (ROS), and the ROS positive rate of the bacteria increased under the treatment with 0.2 mmol/L of Cr(VI) and 250 nmol/L of F-53B, respectively. In addition, the activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced for scavenging ROS in the bacteria that were exposed to Cr(VI) and F-53B. As an antioxidant, rutin was used to repair the toxicity of Cr(VI) and F-53B towards the biofilm formed by the bacteria. When rutin was added to the bacteria medium, with either Cr(VI) or F-53B as pollutant, or with the combined pollutants, the extracellular protein content of the bacteria recovered to 0.84, 0.94, and 0.85 times that of the control, respectively. Meanwhile, the accumulation of ROS and the activities of SOD and CAT decreased, which indicated that the addition of rutin can alleviate the oxidative stress and promote the antioxidant stress system.
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