Foods
·
2024
·Vol. 13
·期 2
·305
·
IF 4.7
Shouchun Liu,Luyao Zhang,Jing Chen,Zhuyi Li,Meijiao Liu,Pengzhi Hong,Saiyi Zhong,Haifeng Li
The effect of temperature fluctuations on the freshness of shrimp in simulated trays was investigated by setting a freeze–thaw (F-T) cycle of 12 h after freezing at −20 °C and thawing at 1 °C under refrigeration. The results showed that the shrimp’s physicochemical properties deteriorated to different extents with the increase in F-T cycles. The total colony count of shrimp was 6.07 lg CFU/g after 21 cycles, and the volatile saline nitrogen content reached 30.36 mg/100 g, which exceeded the edible standard. In addition, the sensory quality and textural properties (hardness, elasticity, chewiness, and adhesion) declined to different degrees with increased F-T cycles. LF-NMR and protein property measurements showed that F-T cycles resulted in reduced water holding capacity and protein denaturation, which were the main factors leading to the deterioration of shrimp quality. Furthermore, flavor changes were analyzed using an electronic nose sensor to establish a freshness model. The W1W, W1S, W2S, and W5S sensors were correlated with the quality changes in shrimp and used as the main sensors for detecting the freshness ofPenaeus vannamei. As a result, to better maintain the overall freshness, temperature fluctuations should be minimized in sales and storage, and fewer than 8 F-T cycles should be performed.
研究领域:
食品科学
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生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Xianbin Su,Teng Li,Xiaowen Zhu,Peihua Zheng,Huakang Pan,Hui Guo
Nonylphenol (NP) is one of the common pollutants in the environment that have toxic effects on aquatic animals. Nevertheless, little is known about the possible toxicity mechanism of NP on the hepatopancreas of Litopenaeus vannamei . In the present study, the detrimental effects of NP on the hepatopancreas of the L. vannamei were explored at the histological and transcriptomic levels. The findings indicated that after NP exposed for 3, 12, and 48 h, the hepatopancreas histology was changed significantly. Transcriptomic analysis showed that a total of 4302, 3651, and 4830 differentially expressed genes (DEGs) were identified at 3, 12, and 48 h following NP exposure. All these DEGs were classified into 12 clusters according to the expression patterns at different time points. GO and KEGG enrichment analyses of DEGs were also performed, immunological, metabolic, and inflammatory related pathways, including arachidonic acid metabolism (ko00590), the PPAR signaling pathway (ko03320), and the regulation of TRP channels by inflammatory mediators (ko04750) were significantly enriched. Six DEGs were selected for validation by quantitative real-time PCR (qRT-PCR) and the results confirmed the reliability of transcriptome data. All results indicated that NP is toxic to L. vannamei by damaging the histopathological structure and disrupting the biological function. The findings would provide a theoretical framework for lowering or limiting the detrimental impacts of NP on aquaculture and help us to further study the molecular toxicity of NP in crustaceans.
研究领域:
水产养殖学
·
环境毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2021
·
IF 3.216
Shiwei Xie,Dan Wei,Lixia Tian,Yongjian Liu
Bile acid metabolism in aquatic animals usually is suppressed when fish meal (FM) is replaced by plant proteins. An eight weeks feeding experiment was conducted to evaluate the effects of dietary chenodeoxycholic acid (CDCA) supplementation on growth, immune response and intestinal health of Penaeus monodon fed a low FM diet. Three diets were formulated: high FM (25 %) diet (HF), low FM (15 %) diet (LF), and low FM diet supplemented with 0.1 % CDCA (LFC). Shrimp fed Diet LF showed lower weight gain and feed efficiency than those fed Diet HF. Low dietary FM up-regulated the mRNA level of xbp1 , and down-regulated the mRNA levels of sod , iap and NF-κB signaling pathway-related genes ( traf6 , tube ). Dietary supplementation of CDCA up-regulated the expression of relish and anti-oxidative-related genes ( sod and cat ) in the hepatopancreas and midgut. Low dietary FM impaired the intestinal fold and induced the endoplasmic reticulum swollen of intestinal epithelial cell, dietary supplementation of CDCA alleviated this problem. The intestinal microbiota diversity was lower in shrimp fed Diet LF than those fed HF. Dietary supplementation of CDCA in a low FM diet increased the intestinal microbiota diversity by decreasing the relative abundance of dominant phylum Proteobacteria. Low dietary FM increased the relative abundance of a dominant pathogenic bacterium ( Vibrio ), which was decreased by the supplementation of CDCA. These results indicated that dietary supplementation of CDCA in a low FM diet improved the growth performance, immune response and intestinal health of shrimp.
研究领域:
水产养殖学
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营养学
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分子生物学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-9
·
IF 4.011
Shao Yongni,Gu Weimin,Jiang Linjun,Zhu Yiming,Gong Aiping
As an ideal raw material for the production of astaxanthin, H . pluvialis was drawing attention during the last few years, there are some research topics initiated to find out the synthetic pathway of astaxanthin in H . pluvialis . In this study, confocal microscopic Raman technology was utilized to analyze the point-by-point mapping for H . pluvialis , and the visualization of pigment such as carotenoid and astaxanthin content were achieved. By comparing the Raman spectra of H . pluvialis and standard product of astaxanthin, and using the C = C stretching vibration of the Raman intensity as the main indicator for carotenoids, the visual spatial distribution for the carotenoids content was obtained. The MCR-ALS was applied to analyze the Raman data of H . pluvialis , and the information of astaxanthin was extracted to achieve real-time spatial distribution. The visualization of astaxanthin content shows that MCR-ALS is very effective for extracting the information of astaxanthin content from H . pluvialis . By exploring the spatial distribution of carotenoids and astaxanthin contents, analyzing the spatial distribution rules during its growth, Raman spectroscopy technology can be utilized to investigate the internal components of the pigment (ataxanthin, etc.) in H . pluvialis , which make it as an effective methodology to monitor the accumulation and changing mechanism of pigment content in microalgae.
研究领域:
生物化学
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光谱学
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藻类研究
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Gang Wu,Wei Su,Lini Huo,Qing Guo,Jianhua Wei,Haiyi Zhong,Peiyuan Li
Developing bifunctional innovative food packaging for maintaining and monitoring food freshness is crucial for food safety. Here, we prepared tannic acid cinnamaldehyde nanoemulsions through self-assembly and ionic cross-linking between the natural emulsifiers tannic acid and cinnamaldehyde, and were incorporated into chitosan as a protective outer layer. Sodium alginate anchored with alizarin was employed as the sensing inner layer. A pH-sensitive bilayer film integrating real-time monitoring and maintenance of food fresh food freshness was designed using layer-by-layer assembly (LBL) technology. The prepared bilayer film exhibited 100 % UV protection, >99 % antimicrobial effect, and 94.86 % and 97.91 % clearance rates for DPPH and ABTS free radicals, respectively. In addition, the bilayer film exhibited high biosafety and sensitive, reversible, and rapid response to pH/NH 3 . Shrimp preservation experiments showed that the smart bilayer film could effectively slow down the growth of microorganisms on the surface of shrimp, extend the freshness period of shrimp, and could monitor the freshness of shrimp in real-time through color changes. In conclusion, the prepared SL-CCT bilayer film has excellent potential for food preservation and freshness monitoring, providing a new perspective for design and development of multifunctional smart food packaging films.
研究领域:
食品科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jinqiu Liu,Bingxue Jiang,Yaoguang Chang,Yanchao Wang,Changhu Xue
Peptides containing branched-chain amino acids (BCAAs) are recognized for their diverse physiological functions; however, effective methods for their target refinement remain underdeveloped. This study introduced a novel approach utilizing the cavity structure of cyclodextrins, along with specific adsorption (50 % ethanol, 50 °C) and crystallization conditions (75 % ethanol, 4 °C), to selectively isolate BCAA-containing oligopeptides from Antarctic krill. Following refinement, the relative BCAA content was elevated to 1.70-fold compared to the original peptide mixture, with the number and abundance of BCAA-containing peptides reaching 95.00 % and 94.96 %, respectively. Principal component and spectral analysis demonstrated that initial ethanol concentration and cyclodextrin cavity size significantly influenced the peptidome profiles by modulating peptide-cyclodextrin interactions. This study provides a comprehensive exploration of the molecular interactions between peptides and cyclodextrins, highlighting a novel application of cyclodextrins for target peptide isolation and offering deeper insights into the principles governing cyclodextrin inclusion.
研究领域:
生物化学
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食品科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Mengxue Ling,Dachao Ma,Qisong Zhong,Cheng Liao,Zheng Liu,Dongbo Wang,Biao Han,Qingge Feng
Plastic and biomass waste are high quality carbon sources for carbon materials. Polyvinyl chloride (PVC) and shrimp shells were selected to investigate the synergistic conversion of co-hydrothermal carbonization and the electrochemical properties of carbon materials. It was found that the particle agglomeration of PVC plastics could be effectively avoided, thus, the dechlorination from UPVC tubes and shrimp shells at 220 °C for 120 min (HUPS 220–120) was up to 100.9 wt%, which was four times higher than solo hydrothermal treatment of UPVC tubes. The dissolution of calcium and magnesium in PVC plastics or shrimp shells was positively and linearly related to the removal of chloride ions on PVC plastics, with the molar ratio close to 1:2. The hydrolysis of shrimp shells catalyzed by hydrogen chloride (HCl), providing abundant nucleophilic groups, which in turn substituted for chlorine on PVC to form polyethers and polyamines. Interestingly, the co-hydrothermal treatment induced O and N functional groups on the hydrochars to maintain and evolve into C O and pyridinic-N after further carbonization and activation. With the features of large specific surface (1161.3 m 2 ·g −1 ), numerous microporous (87.66 %), high graphitization degree (I D /I G =0.85), and abundant C O and pyridinic-N, the carbon materials derived from hydrochar (CHPS 220–60) demonstrated strong charge storage capacity, good conductivity, and multiple redox potentials, raising the capacitive performance as high as 277.5 F·g −1 at 0.5 A·g −1 . This work aims to provide an effective method not only for the upcycling of PVC plastics and shrimp shells but also for the preparation of energy storage materials.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 5
·917
·
IF 4.35
Yunhang Liu,Dongwen Fu,Anqi Bi,Siqi Wang,Xiang Li,Xianbing Xu,Liang Song
Antarctic krill oil (AKO) with 5–30% (w/w) dilution by soybean oil was co-emulsified by phospholipids (PLs) naturally present in AKO and 2% (w/w) casein in the aqueous phase to prepare high internal phase emulsions (HIPEs). The results showed that raising the AKO level resulted in concave-up changes in the mean size of oil droplets which became more densely packed. Confocal laser scanning microscopy (CLSM) and cryo-scanning electron microscopy (cryo-SEM) micrographs revealed that PLs at higher concentrations expelled more casein particles from the oil droplet surface, which facilitated the formation of a crosslinked network structure of HIPEs, leading to reduced mobility of water molecules, extended physical stability, and somewhat solid-like behavior. The rheological analysis showed at lower levels of AKO promoted fluidity of emulsions, while at higher levels it increased elasticity. Lastly, increasing the AKO level slowed down the oxidation of HIPEs. These findings provide useful insights for developing HIPEs of highly viscous AKO and its application in foods.Keywords:high internal phase emulsions;Antarctic krill oil;phospholipids;casein;co-emulsifier
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2020
·
IF 4.3
Jiechen Ma,Wei Huang,Xuesheng Zhang,Yucheng Li,Ning Wang
The target of this research was to explore the adsorption characteristics and mechanisms for the adsorption of copper and cadmium by lobster shell biochar. Two biochars were produced from lobster shells through pyrolysis at 300 and 600℃ (labeled LS300 and LS600). The kinetics and isotherm adsorption curves of Cu 2+ and Cd 2+ on these lobster shell biochars were determined. The adsorption kinetic curves were best depicted by the two-compartment model, and the Toth model was suitable for the isotherm data of Cu 2+ and Cd 2+ . Compared with those of LS300, the adsorption capacities of LS600 for Cu 2+ and Cd 2+ were greater. The original biochar and Cu 2+ or Cd 2+ -loaded biochar were analyzed by SEM-EDS, FTIR, and XRD to explore the adsorption mechanisms. Ion exchange with cations, precipitation with minerals, and interaction with functional groups were the potential mechanisms for the adsorption of Cu 2+ and Cd 2+ by the lobster shell biochars. The contribution rates of these three mechanisms were also calculated. The adsorption mechanism of Cu 2+ onto the prepared biochar was dominated by ion exchange, while cation exchange and interaction with functional groups were the primary adsorption mechanisms of LS300 and LS600 for Cd 2+ , respectively. Moreover, the adsorption capacity of lobster shell biochar was higher than that of other reported biochars. The results of this study indicate that lobster shell biochar is a highly effective adsorbent for the removal of Cu 2+ and Cd 2+ from contaminated water.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Lubricants
·
2025
·Vol. 13
·期 3
·103
·
IF 2.9
Wei Li,Huanyi Chen,Long Chen,Shanqin Ge,Qingzhao Cai,Genxiang Gong,Jinhong Yu,Kazuhito Nishimura,Nan Jiang,Tao Cai
Hydraulic technology has been instrumental in the extensive application of offshore mechanical equipment, particularly in drilling platforms and ships, where high-performance hydraulic fluids are essential for safe and efficient operations. Addressing the urgent need for water-based hydraulic fluids as an alternative to traditional oil-based fluids, this study introduces a novel water-based hydraulic fluid fortified with phytic acid, derived from plant seeds, to achieve low biotoxicity, low coefficient of friction, and reduced frictional heat generation. The integration of phytic acid has significantly enhanced the lubricating performance, reducing the average coefficient of friction to as low as 0.013, as tested by the four-ball tester, which is the lowest value reported to date. Real-time monitoring of the temperature rise of the friction testing apparatus using an infrared thermal imager revealed a 78.6% reduction in temperature increase. Acute toxicity assays using Brine Shrimp demonstrated that the 96 h LC50 value for the water–glycol flame-resistant hydraulic fluid with added phytic acid exceeded 26,304 mg/L, indicating low toxicity. Characterization analyses elucidated the mechanisms underlying the improved tribological properties, highlighting the potential of this eco-friendly fluid for safe and efficient offshore operations.
研究领域:
海洋工程
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Guang Gao,Lin Gao,Qianqian Fu,Xinshu Li,Juntian Xu
Integrated multi-trophic aquaculture (IMTA) has been proposed as a potential solution to supply aquatic food in an environmentally friendly way. However, little is known regarding the impacts of IMTA on the growth rates and food quality of both animals and plants, as well as the seasonal effects. In this study, we conducted field mesocosm experiments using monoculture and coculture systems with the shrimp Litopenaeus vannamei and the macroalga Ulva linza for four weeks in different seasons (autumn, spring and summer) to address the research gap. To evaluate the environmental pollution caused by shrimp culture, there was no water exchange for culture systems including shrimps. Compared to monoculture of L. vannamei , coculture with U. linza significantly reduced dissolved inorganic nitrogen (DIN, 98.5–99.0%) and dissolved inorganic phosphorus (DIP, 98.2–98.8%) but enhanced dissolved oxygen (DO, 56.2–68.7%) and pH (10.7–18.6%) by the end of culture. Compared to monoculture, coculture stimulated the growth rates of L. vannamei by 38.1–58.8% and U. linza by 241.9–290.4% in all seasons, and increased the lipid content of L. vannamei by 24.2% in autumn and by 37.9% in summer and the contents of protein and ash of U. linza by 23.8–29.0% and by 27.6–68.6%, respectively, in all seasons. Coculture enhanced the content of most amino acids in U. linza and the content of total fatty acids (FA) and polyunsaturated fatty acids in L. vannamei in all seasons in comparison with monoculture. In addition, coculture lifted swelling capacity by 28.9–40.5%, water holding capacity by 39.8–43.3% and oil holding capacity by 31.4–32.4% for U. linza in autumn and summer. Apart from relieving eutrophication , deoxygenation and acidification, IMTA increased growth rates of both shrimp and Ulva and improved functional properties of U. linza , suggesting a green and productive aquaculture mode.
研究领域:
水产养殖学
·
环境科学
✓ 已验证
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 6
·876
·
IF 5.561
Yu Li,Siyuan Fei,Deyang Yu,Lijuan Zhang,Jiaxuan Li,Ronggang Liu,Mingqian Tan
Pickering emulsions stabilized from natural sources are often used to load unstable bio-active ingredients, such as astaxanthin (AXT), to improve their functionality. In this study, AXT-loaded Pickering emulsions were successfully prepared by 2,2,6,6-tetramethy-1-piperidine oxide (TEMPO)-oxidized cellulose nanofibers (TOCNFs) fromUndaria pinnatifida. The morphology analysis showed that TOCNFs had a high aspect ratio and dispersibility, which could effectively prevent the aggregation of oil droplets. The stable emulsion was obtained after exploring the influence of different factors (ultrasonic intensity, TOCNFs concentration, pH, and ionic strength). As expected, AXT-loaded Pickering emulsions showed good stability at 50 °C and 14 days of storage. The results of simulatedin vitrodigestion showed that the emulsions exhibited higher release of free fatty acids (FFAs) and bioaccessibility of AXT than those in sunflower oil. Hence, our work brought new insights into the preparation of Pickering emulsions and their applications in protection and sustained, controlled release of AXT.Keywords:Undaria pinnatifida;nanocellulose;Pickering emulsion;stability;astaxanthin
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）