BIOMEDICAL CHROMATOGRAPHY
·
2015
·Vol. 29
·期 11
·1737-1743
·
IF 1.723
Xue-jia Zhai,Fen Chen,Chao-ran Zhu,Yong-ning Lu
Many amino acid neurotransmitters in urine are associated with chronic stress as well as major depressive disorders. To better understand depression, an analytical LC-MS/MS method for the simultaneous determination of 11 underivatized neurotransmitters (4-aminohippurate, 5-HIAA, glutamate, glutamine, hippurate, pimelate, proline, tryptophan, tyramine, tyrosine and valine) in a single analytical run was developed. The advantage of this method is the simple preparation in that there is no need to deconjugate the urine samples. The quantification range was 25–12,800 ng mL −1 with >85.8% recovery for all analytes. The nocturnal urine concentrations of the 11 neurotransmitters in chronic unpredictable mild stress (CUMS) model rats and control group ( n = 12) were analyzed. A series of significant changes in urinary excretion of neurotransmitters could be detected: the urinary glutamate, glutamine, hippurate and tyramine concentrations were significantly lower in the CUMS group. In addition, the urinary concentrations of tryptophan as well as tyrosine were significantly higher in chronically stressed rats. This method allows the assessment of the neurotransmitters associated with CUMS in rat urine in a single analytical run, making it suitable for implementation as a routine technique in depression research. Copyright © 2015 John Wiley & Sons, Ltd.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2014
·
IF 1.2
Jie-Ping Fan,Lie Li,Ze-You Tian,Chun-Fang Xie,Yuan Xue,Yan-Long Xie,Rui Xu,Yu Qin,Xue-Hong Zhang,Jian-Hang Zhu
Uniformly sized synephrine molecularly imprinted polymer (MIP) microparticles were prepared via precipitation polymerization. The presence of the template, the amount of monomer, crosslinker, and porogenic solvent could affect the size distribution and morphology of the polymers. The pseudo-second-order kinetic model could provide a better correlation for the adsorption than the pseudo-first-order model. The intraparticle diffusion study showed that the sorption involved intraparticle diffusion, but that was not the only rate-controlling step. The equilibrium data better fit the Freundlich model than the Langmuir model. The Scatchard plot of MIPs revealed MIPs had two groups of sites with different affinities. Subsequent recognition selectivity experiments demonstrated preferential structural selectivity for synephrine with respect to other structurally similar compounds (i.e., octopamine and tyramine). Compared with the bulk MIP of SYN, the SYN MIP microparticles in this work had higher adsorption selectivity and capacity due to their smaller particle sizes; moreover, the precipitation polymerization was time-saving due to not using a crash-and-sieve process.
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xueli Wang,Xueting Liu,Chunmei Sun,Yanwei Cheng,Zhen Li,Shuyi Qiu,Yongguang Huang
Daocai is a traditional salted pickle in the southeastern region of Guizhou with a unique aroma, color, and taste. The quality of Daocai is greatly influenced by the fermentation temperature. In this study, high-throughput sequencing and headspace-gas chromatography-ion mobility spectrometry were used to investigate the changes in microbial community succession and volatile flavor compounds during Daocai fermentation under temperature-controlled (D group) and non-temperature-controlled (C group).We found that the predominant genera in the C group samples were Latilactobacillus (40.57 %), Leuconostoc (21.25 %), Cystofilobasidium (22.12 %), Vishniacozyma (23.89 %), and Leucosporidium (24.95 %), whereas Weissella (29.39 %), Lactiplantibacillus (45.61 %), Mucor (68.26 %), and Saccharomyces (23.94 %) were the predominant genera in the D group. A total of 92 VFCs were detected in Daocai samples, including 5 isothiocyanates, 16 esters, 14 alcohols, 24 aldehydes, 17 ketones, 3 acids, 2 pyrazines, 1 pyridines, 1 thiazoles, 3 furans, 4 alkenes, and 2 nitriles. Further analysis revealed Latilactobacillus , Leuconostoc , Lactococcus , Cystofilobasidium , Leucosporidium , Holtermanniella , and Dioszegia as key bacteria involved in flavor formation. They are closely related to the formation of flavors such as aldehydes, furans, pyridines, and alkenes. This study contributes to our understanding of the relationship between bacterial communities and the flavor formation during Daocai fermentation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2024
·
IF 3.7
Jiao Feng,Runyuan Jin,Shasha Cheng,Hui Li,Xin Wang,Kequan Chen
In this study, we designed an artificial pathway composed of tyramine β-hydroxylase (TBH) and phenylethanolamine N-methyltransferase (PNMT) for the biosynthesis of both octopamine and synephrine. As most TBH and PNMT originate from eukaryotic animals and plants, the heterologous expression and identification of functional TBH and PNMT are critical for establishing the pathway in mode microorganisms like Escherichia coli. Here, three TBHs were evaluated, and only TBH from Drosophila melanogaster was successfully expressed in the soluble form in E. coli. Its expression was promoted by evaluating the effects of different expression strategies. The specific enzyme activity of TBH was optimized up to 229.50 U·g–1, and the first step in the biosynthetic pathway was successfully established and converted tyramine to synthesize 0.10 g/L of octopamine. Furthermore, the second step to produce synephrine from octopamine was developed by screening PNMT, enhancing enzyme activity, and optimizing reaction conditions, with a maximum synephrine production of 2.02 g/L. Finally, based on the optimization of the reaction conditions for each individual reaction, the one-pot cascade reaction for synthesizing synephrine from tyramine was constructed by combining the TBH and PNMT. The synthetic synephrine reached 30.05 mg/L with tyramine as substrate in the two-step enzyme cascade system. With further optimization and amplification, the titers of octopamine and synephrine were increased to 0.45 and 0.20 g/L, respectively, with tyramine as substrate. This work was the first achievement of the biosynthesis of octopamine and synephrine to date.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lian Xia,Feng Luo,Xiaojuan Niu,Yue Tang,Yuangen Wu
Pb(II) is a prevalent heavy metal ion classified as a 2B carcinogen. Excessive intake of Pb(II) in the human body can damage the central nervous system, kidneys, liver, and immune system, leading to permanent brain damage, anemia, and cancer. Colorimetry can be applied to rapidly determine Pb(II) residues, but there are still many challenges in the accuracy and sensitivity of detection. Based on the inhibitory impact of Pb(II) on the oxidase-like activity of octahedral silver oxide (Ag 2 O), a colorimetric sensor with smartphone-assisted analysis for the Pb(II) detection was first developed. Herein, it has been found that Pb(II) can adsorb onto the surface of octahedral Ag 2 O, hindering the production of O 2 − in the reaction system. This ultimately results in the suppression of oxidase-like activity, leading to a lighter purple appearance of the colorimetric reaction solution. The sensor exhibits a high degree of sensitivity and a limit of detection (LOD) for Pb(II) was calculated as 2.2 μg L −1 . Hence, the developed colorimetric sensor with high sensitivity, excellent specificity, and high tolerance to sodium ions is hopeful to have practical applications in Pb(II) detection in environmental water samples. Moreover, the sensor will provide a novel strategy for heavy metal ion detection and other substances.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2025
·Vol. 2025
·期 1
·6298724
·
IF 2.5
Niuniu Bai,Jianlin He,Bihong Hong,Zelong Wu,Liping Wang,Zongze Shao
Background: Traditional fish sauce is made from fish that have been coated in 30%–40% sea salt and fermented for up to 2 years. The preparation requires a long time, and the fish source contains considerable biogenic amines. In this report, by measuring the growth curve and identifying the intracellular enzyme and the activity of protease, B. zhangzhouensis with the highest activity of protease production was selected from eight strains, which were applied to the fermentation process of fish sauce. Results: Optimized by response surface methodology, the content of amino nitrogen was taken as the response value, and the best fermentation conditions were 4.15% inocula, pH 9.13, and 36.3°C. Under the optimized conditions, fish sauce samples were obtained by fermentation with B. zhangzhouensis for 15 h, 3 days, 7 days, 15 days, and 30 days, respectively. The contents of amino nitrogen, total nitrogen, biogenic amine, and various flavor substances were determined and compared with the traditional 2-year-fermented fish sauce. As a result, the 7-day-fermented fish sauce using B. zhangzhouensis had a similar composition of flavor substances to traditional fish sauce, but a much lower level of biogenic amines. Conclusion: The new method of preparing fish sauce with a fermentation condition of 4.15% B. zhangzhouensis , pH 9.13, and 36.3°C can improve the production efficiency of fish sauce, and the flavor is similar to that of traditional fish sauce.
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2024
·
IF 9.3
Hao-Xin Niu,Tian-Mo Yang,Xin Wang,Ping Zhang,Wenwen Guo,Lei Song,Yuan Hu
Epoxy thermosets are used in many fields because of their outstanding properties. However, commercial epoxy thermosets are flammable, so epoxy resins with excellent flame retardant properties need to be prepared. In recent years, carbon fiber epoxy composites have been widely used in aerospace and other high-tech fields due to their high strength and excellent performance. With the increased use of carbon fiber epoxy composites, the recycling of carbon fibers is an important issue. Herein, a bio-based epoxy vitrimer (TVEP/DFDA) containing a Schiff base structure was successfully prepared by utilizing vanillin, tyramine and furfurylamine. Moreover, carbon fiber epoxy composites (CF-TVEP/DFDA) were successfully prepared. The TVEP/DFDA composite has better mechanical properties than DGEBA/DDM and excellent flame-retardant performance. The limiting oxygen index (LOI) of TVEP/DFDA was 28.5%, and TVEP/DFDA achieved a UL-94 V-0 rating. Heat hazards and smoke hazards are sources of danger in fires. According to the cone calorimetry test, compared with those of DGEBA/DDM, the heat release rate, total heat release and total smoke production of TVEP/DFDA decreased by 73.9%, 52.6% and 56.6%, respectively. The significantly reduced heat and smoke production indicate that TVEP/DFDA has a lower combustion intensity and better fire safety than DGEBA/DDM. CF-TVEP/DFDA also exhibited excellent flame-retardant performance, with a high LOI of up to 45% and a UL-94 V-0 rating. However, the CF-DGEBA/DDM cannot pass the vertical burning test. TVEP/DFDA has excellent stability under different solvents and can be degraded under acidic conditions. This work describes a strategy for recycling carbon fibers by immersion in a hydrochloric acid solution. The recycled carbon fibers exhibit surface morphologies, chemical structures and mechanical properties similar to those of virgin carbon fibers.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xixiang Xie,Junxiang Zeng,Xianli Lan,Xiyu Liu,Jian He,Pan Wu,Yan Huang,Mengqi Chen,Meiyan Wu,Guiyin Li,Liang Shi,Yong Huang
As cholesterol is very intimately linked to the development of many types of cardiovascular diseases, clinical medicine must have a straightforward and precise method for determining total serum cholesterol (TC). Nowadays, enzymatic methods are routinely used in laboratories for the determination of TC, using cholesterol oxidase and esterase reagents for quantitative analysis on fully automated instruments. In this work, an electrochemical biosensor was developed for highly sensitive detection of cholesterol by modified modification of screen-printed carbon electrode (SPCE) and reduction of peroxidase-mimetic catalytic activity of reduced graphene oxide-hemin (RGO-hemin) nano-enzyme. The hydrolysis of cholesterol produced H 2 O 2 when it was subjected to the actions of cholesterol oxidase (CHOD), cholesterol esterase (CHER), and RGO-hemin. In the presence of H 2 O 2 , ferrocene-tyramine (Fc-Tyr) triggers catalytic deposition amplification effect on the SPCE surface, a great deal of electroactive ferrocene marker accumulates on the electrode surface, and Fc-Tyr is measured by an electrochemical workstation. The redox electrical signal detects the concentration of cholesterol in the sample. The biosensor had an operating range of 0.1∼5 mg/mL and a sensitivity of 24.43 μg/mL with acceptable specificity. The electrochemical cholesterol biosensor sensitively captured cholesterol signals in a complex body fluid environment and showed results consistent with clinical trials, with 96.63∼107.32 % recovery and 0.78∼5.28 % relative standard deviation (RSD). Therefore, there is a significant chance that the cholesterol biosensor by the institute will be used in clinical settings to detect serum cholesterol.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shen Zhang,Xiao Wang,Yutong Wang,Jiaxu Dong,Yixuan Zhang,Manlin Zhao
Vitamin B12 is a drug that can participate in the production of bone marrow red blood cells, prevention of pernicious anemia and damage to brain nerves. It is needful to prepare new vitamin B12 determination strategies with excellent sensitivity, selectivity and uniqueness. Herein, a novel and facile chemical reduction method was successfully carried out (histidine and ascorbic acid were applied as protectant and reductant) for preparing histidine-functionalized silver nanoclusters (Ag NCs@his) with blue fluorescence. Some technologies were adopted to clarify the structure, optical characteristics of this stable Ag NCs@his and fluorescence quenching mechanism. Based on inner filter effect, a sensitive and selective platform for vitamin B12 detection was established. Under optimum pH value, reaction time and temperature, the as-developed platform could be utilized to the trace measurement of vitamin B12 with linear range of 0.5–200 μM and detection limit of 0.038 μM. Moreover, this platform was successfully used to measure vitamin B12 in real samples and temperature.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Gan Wu,Qi Cheng,Zhaoyang Ding,Jing Xie
Biogenic amines are critical indicators for the quality and safety of aquatic products, and biogenic amine poisoning could threaten human health. Therefore, it is crucial to develop easily operated and efficient analytical sensors for strict tyramine detection. To address this need, ratiometric fluorescent sensors have been developed for the detection of tyramine. F8BT-RBI polymer dots and PVK-FITC polymer dots were prepared by the semiconductor polymers Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (F8BT) and Poly (9-vinylcarbazole) (PVK), combined with rhodamine B isothiocyanate (RBI) and fluorescein isothiocyanate (FITC) through reprecipitation methods. The ratiometric fluorescent sensors rely on the changes in fluorescence intensity in dual channels caused by the intramolecular charge transfer (ICT) resulting from the reaction between isothiocyanate dyes and tyramine. This approach enables accurate detection of tyramine content within specific linear ranges. For instance, PF8BT-RBI covers a range of 2.74–20.6 mg L −1 with a limit of detection (LOD) of 0.14 mg L −1 , while PPVK-FITC covers 20.6–61.7 mg L −1 with an LOD of 0.59 mg L −1 . PF8BT-RBI and PPVK-FITC had potential for fluorescence detection of tyramine, and were applied in the detection of tyramine in aquaculture water and tuna. By utilizing Pdots and isothiocyanate dyes, these sensors can meet the demand for ensuring the freshness and safety of aquatic products.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xia Xu,Xinyu Wang,Yicheng Ding,Xuxia Zhou,Yuting Ding
Preserving fish meat poses a significant challenge due to its high protein and low fat content. This study introduces a novel approach that utilizes a common type of lanthanide metal-organic frameworks (Ln-MOFs), EuMOFs, in combination with 5-fluorescein isothiocyanate (FITC) and methylcellulose (MC) to develop fluorescent sensor arrays for real-time monitoring the freshness of fish meat. The EuMOF-FITC/MC fluorescence films were characterized with excellent fluorescence response, ideal morphology, good mechanical properties, and improved hydrophobicity. The efficacy of the fluorescence sensor array was evaluated by testing various concentrations of spoilage gases (such as ammonia, dimethylamine, and trimethylamine) within a 20-min timeframe using a smartphone-based camera obscura device. This sensor array enables the real-time monitoring of fish freshness, with the ability to preliminarily identify the freshness status of mackerel meat with the naked eye. Furthermore, the study employed four convolutional neural network (CNN) models to enhance the performance of freshness assessment, all of which achieved accuracy levels exceeding 93 %. Notably, the ResNext-101 model demonstrated a particularly high accuracy of 98.97 %. These results highlight the potential of the EuMOF-based fluorescence sensor array, in conjunction with the CNN model, as a reliable and accurate method for real-time monitoring the freshness of fish meat.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xiaohan Hua,Tingting Yan,Shuyi Liu,Lijun Yin,Xin Jia
The high protein content in sufu provides a substrate for microorganisms to produce biogenic amines (BAs), thereby posing a potential risk to food safety. The aim of this study was to identify the main BAs produced by different types of sufu (red, white and grey sufu) and investigate the relationship between bacterial diversity and BA production using high-throughput sequencing. The results revealed significant differences in the total BA content of the three types of sufu at the post-fermentation stage, with grey sufu exhibiting the highest total BA content (ranging from 6389.00 mg/kg to 7255.49 mg/kg). The main BAs found in all three types of sufu were tyramine, putrescine and cadaverine. Enterococcus genus produced high levels of tyramine and phenylethylamine in all three types of sufu. The predominant cadaverine-producing bacteria in red sufu was Enterobacter genus, whereas Klebsiella pneumoniae was the primary producer of cadaverine in white and grey sufu. Putrescine was primarily associated with the Bacillus and Enterobacter genera in red and grey sufu. Acinetobacter , Enterococcus and Enterobacter were the common dominant genera during the post-fermentation stage of the three kinds of sufu, which were positively correlated with the production of various BAs. These findings will help to understand the mechanism of BAs production by bacterial communities in different types of sufu to ensure safe production.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）