MOLECULES
·
2023
·Vol. 28
·期 3
·1459
·
IF 4.6
Xinying Zhang,Xialing Hou,Decheng Lu,Yingying Chen,Lingyan Feng
Porphyrin (TMPyP) functionalized carbon quantum dots (CQDs-TMPyP), a novel and efficient carbon nanocomposite material, were developed as a novel luminescent material, which could be very useful for the sensitive detection of copper ions in the Cu2+quenching luminescence of functionalized carbon quantum dots. Therefore, we constructed a sensitive “signal off” ECL biosensor for the detection of Cu2+. This sensor can sensitively respond to copper ions in the range of 10 nM to 10 μM, and the detection limit is 2.78 nM. At the same time, it has good selectivity and stability and a benign response in complex systems. With excellent properties, this proposed ECL biosensor provides an efficient and ultrasensitive method for Cu2+detection.Keywords:carbon quantum dots;porphyrin;electrochemiluminescence;Cu2+detection
研究领域:
分析化学
·
纳米材料
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Chang Xu,Yue Feng,Xin-Xin Zhao,Shan Zhao,Shu-Guang Wang,Chao Song
Tetracycline (TC) and Cu(II) have been frequently detected in natural and engineering waters, especially in aquaculture water. However, literatures involving in the influence of Cu(II) on the transformation of TC are still insufficient. In this study, we investigated the photolytic fate of TC induced by Cu(II) under UV irradiation. Cu(II) significantly promoted the removal of TC with photolysis rate constant of 0.467 h −1 at TC:Cu(II) molar ratio = 1, which was 49.04% higher than that without Cu(II) (0.238 h −1 ). Cu(II) exhibited multiple influences on both direct and indirect photolysis of TC. Singlet oxygen ( 1 O 2 ) and hydroxyl radicals (·OH) made a major contribution to Cu(II)-induced TC photolysis, with contribution rates of 24.69% and 12.96%, respectively. Besides, Cu(II) preferred to complex between O20-O21 or O26-O27 to form TC-Cu(II) complexes with complexation ratio of 1. The Condensed Fukui Function and Condensed Double Descriptors values of TC-Cu(II) complexes were calculated for the first time with density functional theory. It showed that TC-Cu(II) complexes were more vulnerable to ROS attack, thus promoting TC degradation. Moreover, Cu(II) greatly changed the photolysis path of TC, mainly through hydroxylation, hydrogen extraction, and deamidation reaction into new byproducts. Furthermore, Cu(II) changed the toxicity of the products via changing the original TC photolysis products. This study provides new insights for the environmental fate of antibiotics, and also has far-reaching implications for wastewater treatment in pharmaceutical and aquaculture industry.
研究领域:
环境科学
·
水处理工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hua Liang,Zongchen Li,Daqiang Wu,Ke Dai,Luyao Zhong,Yonggang Liu,Minhao Yan,Xianguo Tuo
Structure is a crucial factor to decide the selectivity and sensitivity of chemosensors. In this work, a diacylhydrazone 2a and its congeneric derivatives 2b-2d were synthesized and their applications for Al 3+ detection were investigated. Except 2d , all the other compounds exhibit notable turn-on fluorescence toward Al 3+ over 15 other metal ions and the sensitivities of 2b and 2c are even higher after modification, illustrating ortho -OH indispensability in their structures for sensing. Reversibility investigation shows that the sensors all have regeneration ability. Meanwhile, the sensor-Al 3+ systems show selective fluorescence turn-off for Cu 2+ , providing a potential platform for tandem recognition of Al 3+ and Cu 2+ . 2a as a standard, shows a 1:2 molar ratio to Al 3+ confirmed by Job’s plot, 1 H NMR spectra and mass spectra, and its detection limit for Al 3+ is 7.63 nM in ethanol. It has relevant sensitivity over pH 3-9, with the highest sensitivity at around pH 4. Apart from Al 3+ detection, anion selectivity was also explored and it shows that 2a , 2b and 2c all have selectivity toward CN − evidenced by UV-vis spectra.
研究领域:
化学传感器
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 9
·1341-1346
·
IF 2.7
Shuhua Zhao,Lei Shi,Xiufeng Zhang,Xiaoran Sun,Wenxuan Zhu,Lijia Yu
The herbicide glyphosate, N-(phosphonomethyl)glycine, has been widely used in the past 40 years, and has had many adverse effects on human health. Here, we constructed a convenient “on–off–on” fluorescent platform for detection of glyphosate via Cu2+ modulated squaraine dye fluorescence quenching. The squaraine dye F-0 exhibited strong fluorescence, which could be quenched by the addition of Cu2+. However, the addition of glyphosate restored the fluorescence intensity of F-0 due to the formation of a Cu2+-glyphosate complex. F-0 was utilized as a fluorescent probe for the quantitative detection of glyphosate, with the lowest detection limit of 13.16 nmol L−1. Furthermore, this method demonstrated high selectivity and anti-interference capabilities. The successful monitoring of glyphosate in real samples was achieved using this detection strategy.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
·
2024
·
IF 3.2
Wenwei Tang,Zuxun Huang,Yunying Liu,Xinping Zeng
The microbial manganese removal process is believed to consist of the catalytic oxidation of Mn(II) by manganese oxidase. In this study, the multicopper oxidase CopA was purified and exhibited high manganese oxidation activity in vitro, and it was found that Cu(II) can significantly enhance its manganese oxidation activity. Gene site-directed mutagenesis was used to mutate four conserved copper binding sites of CopA to obtain four mutant strains. The manganese removal efficiencies of the four strains were determined, and it was found that H120 is the catalytically active site of CopA. The loss of Cu(II) and the mutation of the conserved copper binding site H120 resulted in the loss of ethoxyformyl and quinone modifications, a reduction in the number of modifications, and a change in the position of modifications, eventually causing a decrease in protein activity from 85.87% to 70.1%. These results reveal that Cu(II) and H120 play an indispensable role in manganese oxidation by the multicopper oxidase CopA. X-ray photoelectron spectroscopy (XPS) analysis indicates that biogenic manganese oxides produced by strains and by CopA were both composed of MnO 2 and Mn 3 O 4 and that the average valence of Mn was 3.2.
研究领域:
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Xiaoyi Lu,Shangshang Zhang,Weiguang Shi,Dan Cheng,Hong-Wei Li,Shihan Xu,Biao Dong
Carbon dots (CDs) are important zero-dimensional materials with excellent luminescent properties. However, the mechanism of CDs photoluminescence is still an open debate, which significantly hinders the development of CDs with desired optical properties. In this work, bright blue fluorescent carbonized 1.0 G polyamine-amine polymer dots (NCPDs) has been prepared by a simple hydrothermal method, with a quantum yield of 57 %. The dominant luminescence of the NCPDs is attributed to the surface states based on synergistic hybridization between the carbon backbones and connected functional groups at below 120 °C. Photoluminescence transforms from the surface states to the surface - carbon core states, and then to carbon core states from 120° to 150 °C. Meanwhile, the degree of carbonization increases along with the size of NCPDs growing from 1.36 to 3.20 nm, and the NCPDs present excitation-dependent emission properties. Due to the remarkable fluorescence property of NCPDs, Cu 2+ determination has been carried out in the aqueous phase, and the detection limit is 9 nM, which is much lower than the international minimum standard (20 μM). NCPDs present high selectivity and sensitivity toward detecting Cu 2+ , and provide potential applications in detecting metal pollution in the environmental water.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Zhiyue Li,Zhiwei Guo,Taoyi Zhang,Quan Li,Jing Chen,Wenxi Ji,Cong Liu,Yun Wei
Synthesizing high uniform metal-organic frameworks grown on natural biomass such as sodium alginate (ALG), which can efficiently remove metal ions from aqueous solution, is a challenging project. A simple and eco-friendly method of preparing ALG@ZIF-67 hydrogels by in situ synthesis of ZIF-67 onto ALG hydrogels was established. Combining the virtues of ALG with abundant hydroxyl and carboxyl groups and ZIF-67 with excellent porous structure, the obtained ALG@ZIF-67 hydrogels have excellent adsorption for Cu 2+ (153.63 mg/g), which was much higher than that of pure ALG hydrogels (111.79 mg/g). The methodology showed that the adsorption process was in accord with the Langmuir isotherm adsorption model (R 2 = 0.9972) and pseudo-second-order kinetic model (R 2 = 0.9822). The thermodynamic experiments demonstrated that Cu (II) was absorbed by hydrogel with an endothermic and spontaneous process. The competitive adsorption experiment results of ALG@ZIF-67 hydrogel indicated that the absorption capacity of Cu (II) was higher than that of Cd (II), Ni (II), Zn (II) and Mn (II). Moreover, the ALG@ZIF-67 hydrogels still had excellent reusability after five adsorption-desorption cycles. The results indicated that the ALG@ZIF-67 hydrogels had the advantages of low cost, easy preparation, and environment friendly.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Bowen Zha,Shuting Fang,Huiling Chen,Hongyu Guo,Fafu Yang
Although some sensors for Cu 2+ and Zn 2+ had been reported, the sensor with long-wavelength emission in aqueous media for in-situ detecting Cu 2+ and Zn 2+ was always expected. Herein, a biphenylacrylonitrile Schiff-base ( OPBS ) with large aromatic conjugated system was designed and synthesized in yield of 82%. OPBS possessed excellent long-wavelength fluorescence at 550–750 nm in aqueous media, which selectively response to sense Cu 2+ with quenched fluorescence and Zn 2+ with chromotropic fluorescence from red to yellow. The detection of Cu 2+ and Zn 2+ were realized without mutual interference in their coexistence system by means of the assistance of ATP. The detection limits were 2.3 × 10 −7 M for Cu 2+ and 1.8 × 10 −6 M for Zn 2+ , respectively. The sensing mechanism was elucidated by binding MS spectra, fluorescence Job’s plot and 1 H NMR spectra. Moreover, OPBS exhibited good bioimaging performance and the in-situ sensing abilities for Cu 2+ and Zn 2+ in living cells, suggesting the application potential for detecting Cu 2+ and Zn 2+ in both vitro assay and vivo environment.
研究领域:
分析化学
·
生物化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2017
·
IF 0
Yunhong Yi,Junliang Lv,Nanjing Zhong,Gongqing Wu
The present study explored the ability of a novel spent chrysanthemum adsorbent prepared from acid acetaldehyde for the removal of Cu 2+ from an aqueous solution. The effect of various parameters, including solution pH, adsorbent dosage, contact time and temperature on adsorption of Cu 2+ were studied by batch experiments. According to the experimental results, the optimal pH range and optimum dosage were found to be 3.0–5.0 and 6.0 g/L, respectively. The adsorption amount at equilibrium decreased with the increase of temperature. Freudlich model provided better correlation for the adsorption of Cu 2+ by modified spent Chrysanthemum (MSC) (R = 0.99)and spent Chrysanthemum (SC)(R = 0.98). Adsorption capacity of MSC was 692 mg/g at 293 K, but it was 400 mg/g at 293 K for SC. The adsorption process on Cu 2+ proceeded rapidly and almost reached adsorption equilibrium within 20 min. The kinetics data of MSC were analyzed using the pseudo-first-order equations and pseudo-second-order equations. The pseudo-second-order equation fitted the experimental data very well (R = 0.99). The adsorption amount at equilibrium decreased with the increase of temperature. The thermodynamic parameters showed that the biosorption was exothermic and spontaneous. Experimental results showed that MSC could be used as an effective biosorbent for Cu 2+ removal from aqueous solutions.
研究领域:
环境科学
·
生物学
✓ 已验证
DOI
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Zhong Wang,Ya Zhao,Yuyu Zhao,Yi Zhang,Xiaohong Yao,Ruiqiang Hang
Copper, a frequently used additive of implant materials, can alter macrophage phenotype thus directing the fate of the implants. Exosomes, secreted by mammalian cells, can target to recipient cells and mediate their functions. However, whether exosomes derived from macrophages upon copper ion stimulation can modulate angiogenesis, a key index for implant osseointegration, is still unclear. Herein, the influence of copper ions on macrophage-derived exosome secretion, ingestion behavior by endothelial cells, and angiogenic-induction ability is investigated. The results show copper ions (0–100 μM) have little influence on the secretion of macrophage-derived exosomes. Endothelial cells can uptake the exosomes from all the groups in a time-dependent manner. The exosomes have little influence on endothelial adhesion and proliferation, but can upregulate angiogenic ability of endothelial cells in vitro and in vivo , which may be related to trafficking of integrin β1. The results provide insight into the effect of copper ions on immunomodulatory mechanism of macrophages, which is important for implant design from the perspective of material compositions.
研究领域:
免疫学
·
材料科学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Guobin Mao,Chen Liu,Mingyuan Du,Yuwei Zhang,Xinghu Ji,Zhike He
Galactose oxidase is a copper-activated enzyme and have a vital role in metabolism of galactose. Much of the work is focused on determining the amount of galactose in the blood rather than measuring the amount of galactose oxidase to urge the galactosemia patients to restrict milk intake. Here, a simple and effective method was developed for Cu 2+ and copper-activated enzyme detection based on homogenous alloyed CdZnTeS quantum dots (QDs). Meso − 2,3-dimercaptosuccinic acid (DMSA) was used as the reducing agent for preparing QDs and the highest quantum yield of CdZnTeS QDs was 69.4%. In addition, the as-prepared CdZnTeS QDs show superior fluorescence properties, such as good photo-/chemical stability. The DMSA was the surface ligand of the QDs, containing abundant -SH and -COOH, thus the surface ligands have a high affinity with Cu 2+ . Therefore, this developed probe can be applied for Cu 2+ and galactose oxidase detection and shows a good sensitivity in the buffer. Then, this probe was successfully used for Cu 2+ and galactose oxidase detection in real samples with the satisfactory results. The proposed fluorescence quenching strategy gives a new and simple insight for enzyme assay without the enzyme-catalyzed reaction.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2015
·Vol. 22
·期 8
·1-10
·
IF 1.92
Li Luzhong,Wang Zhu,Ma Piming,Bai Huiyu,Dong Weifu,Chen Mingqing
A novel bio-adsorbent, composite hydrogel beads, composed of polyvinyl alcohol (PVA), chitosan (CS) and graphene oxide (GO) was prepared by an instantaneous gelation method. The micromorphology and thermal stability of the PVA/CS/GO hydrogel beads were characterized by scanning electronic microscope (SEM) and thermo gravimetric analysis (TGA) respectively. Adsorption of Cu(II) onto PVA/CS/GO hydrogel beads was investigated with respect to pH, initial concentration, temperature and adsorption time. Adsorption data were well matched by Langmuir isotherm and pseudo-second-order kinetic model at the optimum pH 5.5. The maximum adsorption capacities of PVA/CS/GO hydrogel beads were found to be 162 mg g −1 for Cu(II) at 30 °C, which was much higher than that of PVA/CS hydrogel. Thermodynamic studies indicated that the adsorption was spontaneous and endothermic process in nature. Besides, desorption efficiency and reusability of the adsorbents were assessed on basis of six consecutive adsorption-desorption cycles. Based on these studies, it can be seen that PVA/CS/GO hydrogel beads will be a potential recyclable adsorbent for removal of hazardous metal ions in waste water.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）