EUROPEAN JOURNAL OF MEDICAL RESEARCH
·
2024
·
IF 2.8
Yang Chen,Shujun Bao,Zhongzhao Wang,Zheng Fang,Hao Tang
Background Cisplatin (DDP) chemotherapy is commonly used in therapy for non-small cell lung cancer (NSCLC), but increased drug resistance has become a huge obstacle. Baicalin (BA) contributed to the sensitivity of NSCLC to DDP. Here, we aimed to further probe the pathophysiological mechanisms of BA in NSCLC. Methods A549 and A549/DDP cells and xenograft mice were treated with BA and DDP. Xenograft mice were treated additionally with the NRF2 inducer (Bardoxolone methyl, BM) and KEAP1 knockdown. The levels of ferritinophagy-related proteins and biomarkers were determined. The autophagosomes were observed. M1 macrophage polarization and the contents of related indicators were analyzed. The involvement of KEAP1/NRF2/HO-1 was determined. Results BA inhibited cell development, and the effect of BA and DDP on cell development was additive. The abundance of ferritinophagy-related proteins and the number of autophagosomes were induced by BA. BA also promoted the transition of GSH to GSSH. BA favored M1 macrophage polarization and affected the expression of related proteins. When BA and DDP combined, these molecular phenomena were further exacerbated. BA induced accumulation of KEAP1 and reduction of NRF2 and HO-1. However, BM and KEAP1 knockdown disrupted the synergistic effects of BA and DDP on inhibiting NSCLC growth. BM and KEAP1 knockdown reversed DDP and BA-promoted protein expression activity and M1 macrophage polarization. Conclusion Our findings suggest that BA is involved in ferritinophagy and macrophage immunity through the KEAP1-NRF2/HO-1 axis, thereby improving the DDP sensitivity in NSCLC, which could provide new candidates for treatment strategies.
研究领域:
肿瘤学
·
药理学
·
免疫学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2018
·Vol. 35
·期 4
·1700418
·
IF 4.384
Ziyao Liu,Xiaohui Zhan,Xianghui Xu,Yao Wu,Zhongwei Gu
Superparamagnetic iron oxide nanoparticles (SPIONs) have become important tools for the imaging and detecting of prevalent diseases for many years. Scientists usually harness their attraction to a static magnetic field (SMF) to increase targeting efficiency and minimize side effects. To prolong blood circulation time and minimize reticuloendothelial system clearance, SPIONs are increasingly designed with a negatively charged surface. Understanding how a SMF affects the SPIONs with a negative surface charge is fundamental to any potential downstream applications of SPIONs as drug delivery carriers and bio-separation nanoparticles. The goal of our study is to investigate the effect of SMF treatment (204 mT) on the in vitro and in vivo protein corona formed on negatively charged SPIONs. The results reveal that the amount of protein and the composition of protein corona is directly related to the SMF treatment. Compared with the in vivo protein corona, SMF treatment exercises considerable influence on the composition of the in vitro protein corona. The in vitro protein corona formed on SPIONs modulates the secretion of inflammatory cytokines from cells. To the best of our knowledge, this report describes the first demonstration of a SMF as an influencing factor on protein corona formation in vivo. Our results help to elucidate the biological mechanisms of SPIONs with SMF treatment and suggest that the protein corona effect should be considered during the development of a magnetic target.
研究领域:
生物医学工程
·
纳米医学
·
分子生物学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 31
·32345-32359
·
IF 2.914
Yu Binglong,Wang Jie,Yang Xiaoxin,Wang Wenlong,Cai Xiulan
In this paper, we synthesized a polyglycerol(PG)-mediated superparamagnetic graphene oxide nanocomposite called MGON, consisting of PG-modified superparamagnetic iron oxide nanoparticles (SPION) covalently bonded to PG-functionalized graphene oxide (GO). MGON exhibits better dispersibility and colloidal stability in aqueous solution than the magnetic graphene oxide reported in the literature. The physicochemical properties of MGON were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and UV-vis spectroscopy. Applied to the adsorption of tetracycline (TC) in aqueous solution as an adsorbent, the MGON showed excellent adsorption performance with the maximum adsorption capacity of 684.93 mg/g at 298 K. Adsorption kinetics and isotherm results indicate that the adsorption process conforms to the pseudo-second-order kinetics and Langmuir isotherm models. Adsorption thermodynamics has confirmed that the adsorption process of TC on MGON is spontaneous and endothermic. With the increase of temperature, the adsorption capacity of MGON increases continuously, and the adsorption capacity of MGON is the largest when the pH value is 7. Furthermore, the π-π and cation-π interaction, amidation reaction, and hydrogen bonding can be used to explain the adsorption mechanism of TC on MGON. Desorption and regeneration experiments showed that MGON still had 67.65% regenerative performance after five cycles. Hence, MGON is a promising adsorbent in the removal of tetracycline from wastewater.
研究领域:
环境科学
·
纳米材料学
·
污染物吸附
·
水污染治理
·
环境功能材料
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2018
·
IF 3.197
Zhaoliang Wu,Na Li,Xin Zhang,Yanyan Xu,Ting Shu,Wei Liu,Bin Hu
Resveratrol (2,5,4′-trihydroxystilbene) is a naturally occurring phytoalexin produced by some spermatogphytes and it has recently attracted the increasing attention of agronomists and pharmacologists owing to its satisfactory bioactivities. The objective of this work was to develop a cost-effective technology of foam fractionation to recover trans -resveratrol ( trans -Res) from the leaching solution of muscat grape pomace on the assistance of molecular imprinting modified SiO 2 nanoparticles . First, trans -Res was leached from muscat grape pomace through the method of alkaline extraction. Under the suitable leaching conditions, the leaching yield of trans -Res reached 84.34%. Adsorption experiments were performed for describing the adsorption behavior of trans -Res on the molecular imprinting modified SiO 2 nanoparticles . Results indicated that their adsorption could be fairly described by Freundlich model and fitted well with the pseudo-second-order kinetics model. Moreover, the molecular imprinting modified SiO 2 nanoparticles could effectively maintain the stability of trans -Res through avoiding the cis-trans photo-isomerization and oxidative degradation. Finally, foam fractionation was carried out for recovering trans -Res from the leaching solution, in which the concentration of trans -Res was as high as 29.09 mg/L. The molecular imprinting modified SiO 2 nanoparticles were used as the collector. Under the suitable conditions of loading liquid volume 250 mL, particle size of molecular imprinting modified SiO 2 nanoparticles 436.0 nm, sapindus saponin concentration 0.075 g/L, volumetric gas flow rate 100 mL/min and pore diameter of gas distributor 0.180 mm, the enrichment ratio and the recovery percentage of trans -Res reached 13.68 ± 1.03 and 89.73 ± 3.57%, respectively. This work is expected to provide a new technology for selectively recovering non-surface-active materials from the solution with their isomers or analogues, and to facilitate the industrialization of foam fractionation.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2025
·
IF 4.7
Yanmin Zhang,Jianqiang Hu,Yan Zhang,Xiaolin Yang,Weihua Tong,Xinxin Ci
Background The transition from acute kidney injury (AKI) to chronic kidney disease (CKD) is a significant cause of kidney failure. Although the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) has been demonstrated to inhibit ferroptosis and alleviate cisplatin-induced AKI, the specific roles of Nrf2 and the alkaloid leonurine in modulating the AKI-CKD transition remain incompletely elucidated. Methods Murine and human proximal tubular epithelial cell models of the AKI-CKD transition were established using repeated low-dose cisplatin (RLDC) to investigate the roles of Nrf2 and leonurine in ferroptosis regulation and disease progression. Key results In mice, RLDC administration induced persistent tubular injury, ferroptosis, and progressive renal fibrosis. In HK-2 cells, RLDC triggered oxidative stress and ferroptosis. Although Nrf2 expression was transiently upregulated during the acute phase (4 weeks post-RLDC), it was downregulated by the chronic phase (8 weeks post-RLDC), correlating with impaired antioxidant capacity and exacerbated ferroptosis. Nrf2 knockout mice exhibited more severe oxidative damage, ferroptosis, and renal dysfunction following RLDC challenge. Pharmacological activation of Nrf2 by leonurine attenuated oxidative injury and ferroptosis and promoted tubular repair. Leonurine also suppressed the epithelial–mesenchymal transition and aberrant extracellular matrix deposition, ultimately mitigating renal fibrosis. The protective effects of leonurine were abolished in Nrf2 knockout mice. Conclusion These findings demonstrate that Nrf2 activation mitigates cisplatin-induced AKI-CKD transition by counteracting oxidative stress and ferroptosis, thereby attenuating renal fibrosis. Leonurine, as a potent Nrf2 activator, represents a promising therapeutic agent for preventing AKI-CKD transition.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2012
·
IF 4.525
Bing-Qiang Lu,Ying-Jie Zhu,Hai-Yong Ao,Chao Qi,Feng Chen
The synthesis of the mesoporous nanocomposites consisting of magnetic iron oxide nanoparticles and calcium silicate with uniform size has been a challenge, although they are the ideal potential agent for medical diagnosis and therapy. In this work, the core/shell structured mesoporous nanocomposites consisting of magnetic iron oxide nanoparticles as the core and calcium silicate as the shell have been successfully synthesized using a two liquid phase system by ultrasound irradiation, in which the hydrophobic phase is composed of hydrophobic Fe3O4 nanoparticles and tetraethyl orthosilicate (TEOS), and the water phase consists of Ca(NO3)2, NaOH, and water. The hollow mesoporous nanocomposites consisting of magnetic iron oxide nanoparticles and calcium silicate are obtained by adding a certain amount of the inert hydrophobic solvent isooctane in the reaction system before ultrasound irradiation. The nanocomposites have a superparamagnetic behavior, high Brunauer–Emmett–Teller (BET) specific surface area (474 m2 g–1), and high Barrett–Joyner–Halenda (BJH) pore volume (2.75 cm3 g–1). The nanocomposites have high drug loading capacities for bovine hemoglobin, docetaxel, and ibuprofen. The docetaxel-loaded nanocomposites have the anticancer ability and, thus, are promising for applications in biomedical fields.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhemin Chen,Yongping Pu,Yating Ning,Chunhui Wu,Lei Zhang,Bo Wang,Xuqing Zhang
Eco-friendly dielectric ceramics with excellent energy storage performance and dielectric temperature stability are the kernel parts of pulsed power devices in the next-generation. However, the contradiction between high breakdown electric field ( E b )and maximum polarization ( P max ) restricts the improvement of comprehensive performance. Herein, we report a novel strategy of entropy engineering in the superparaelectric state to address the above concerns. On the one hand, near-linear polarization response can be achieved in the superparaelectric state, which can be beneficial to obtain a large P max , low remnant polarization ( P r ) and slim P-E loops. On the other hand, the improvement of configuration entropy (Δ S config ) is conducive to refining grain size and domain size, enhancing bandgap width, and suppressing leakage current and interfacial polarization, thus the generation of large E b . Therefore, with the Δ S config increases from 0.98 R to 1.44 R, a giant energy storage density ( W rec ) of 8.11 J/cm 3 as well as a high efficiency ( η ) of 90.2 % are synchronously obtained. Significantly, the optimum sample shows outstanding dielectric temperature stability (Δ C / C 25°C ≤ ± 15 %, −55 ∼ 165 °C) in accordance with X8R. This work demonstrates that the medium entropy superparaelectrics are a shortcut to achieve next-generation ceramic capacitors with high comprehensive performance.
研究领域:
材料科学
·
电子工程
✓ 已验证
DOI
高影响因子
AAPS PHARMSCITECH
·
2018
·Vol. 19
·期 5
·2048-2057
·
IF 2.666
Zhai Qingqing,Li Hailong,Song Yanlin,Wu Ruijiao,Tang Chuanfang,Ma Xiaodong,Liu Zhihao,Peng Jinyong,Zhang Jianbin,Tang Zeyao
This work aimed to develop and optimize several lipid nanocapsule formulations (LNCs) to encapsulate cisplatin (CDDP) for treatment of hepatocellular carcinoma. By comparing the effect of oil/surfactant ratio, lecithin content, and oil/surfactant type on LNC characteristics, two LNCs were selected as optimal formulations: HS15-LNC (Solutol HS 15/MCT/lecithin, 54.5:42.5:3%, w / w ) and EL-LNC (Cremophor EL/MCT/lecithin, 54.5:42.5:3%, w / w ). Both LNCs could effectively encapsulate CDDP with the encapsulation efficiency of 73.48 and 78.84%. In vitro release study showed that both LNCs could sustain the release CDDP. Moreover, cellular uptake study showed that C6-labeled LNCs could be effectively internalized by HepG2 cells. Cellular cytotoxicity study revealed that both LNCs showed negligible cellular toxicity when their concentrations were below 313 μg/mL. Importantly, CDDP-loaded LNCs exhibited much stronger cell killing potency than free CDDP, with the IC50 values decreased from 17.93 to 3.53 and 5.16 μM after 72-h incubation. In addition, flow cytometric analysis showed that the percentage of apoptotic cells was significantly increased after treatment with LNCs. Therefore, the prepared LNC formulations exhibited promising anti-hepatocarcinoma effect, which could be beneficial to hepatocellular carcinoma therapy.
研究领域:
药学
·
肿瘤学
·
纳米技术
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Huikang Yang,Nianhua Wang,Lei Mo,Mei Wu,Ruimeng Yang,Xiangdong Xu,Yugang Huang,Jiantao Lin,Li-Ming Zhang,Xinqing Jiang
Tumor-targeted multifunctional nanocarriers play an important role in tumor diagnosis and treatment. Herein, disulfide bonds linked amphiphilic hyaluronan-SS-poly(ε-caprolactone) diblock copolymers (HA-SS-PCL) were synthesized and studied as theranostic nanocarriers for tumor diagnosis and treatment. The chemical structure of HA-SS-PCL was confirmed by Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance ( 1 H NMR). The self-assembling behavior of the HA-SS-PCL into GSH-responsive micelles and their degradation were characterized by fluorescence spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Theranostic nanocarriers encapsulating doxorubicin (DOX) and superparamagnetic iron oxide (SPIO) were formed via a dialysis. In vitro drug release results suggested that the HA-SS-PCL micelles possessed reductant-triggered doxorubicin release ability, which was confirmed by 100% of DOX release from HA-SS-PCL micelles within 12 h under 10 mM of glutathione (GSH), whereas about 40% of DOX was released under non-reductive condition within 24 h. Both flow cytometry and confocal laser scanning microscopy (CLSM) analysis revealed that the HA-SS-PCL micelles loaded with DOX were internalized in HepG2 cell via a receptor mediated mechanism between hyaluronan and the CD44 receptor. Furthermore, the MTT assay and cell apoptosis analysis revealed that the DOX-loaded HA-SS-PCL micelles exhibited pronounced antitumor ability towards HepG2 cells compared with that of the reduction-insensitive HA-PCL micelles at the same DOX dosage. The r 2 relaxivity value of the DOX/SPIO loaded HA-SS-PCL micelles was up to 221.2 mM −1 s −1 (Fe). Thus, the obtained HA-SS-PCL block copolymers demonstrate promising potential as tumor targeting theranostic nanocarriers in the field of tumor diagnosis and treatment.
研究领域:
生物材料
·
肿瘤学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ying-Lin Zhu,Wei-Xuan Li,Yu-Hui Zhang,Han Yan,Long-Yu Guo,Yue Zhang,Hai-Peng Lv,Ling-Hong Zhou,Zhi Lin,Wen-Liang Wu,Yin Zhu
Jianghua Kucha (JHKC) is an ancient tea germplasm, which presents a high value for resource utilization but was less developed in aroma research. In this study, volatile metabolites in fresh leaves and corresponding black teas of JHKC, along with odorants of JHKC black teas were systematically investigated using stir-bar sorptive extraction in combination with gas chromatography–mass spectrometry (GC–MS) and GC–olfactometry/MS (GC–O/MS) technologies. A total of 128 and 135 volatile compounds were identified in fresh leaves and black teas, respectively, along with 13 key differential volatile components derived from fatty acids, pigments, glycoside precursors, and the terpene pathway. An odorant wheel for JHKC black teas was developed based on 29 important odorants determined through a combination of GC–O/MS perception, aroma extract dilution analysis, odor activity value measurement. Trans - β -Damascenone, cis -jasmone, and cis -jasmin lactone have been identified as significant contributors to the “sweet” flavor of JHKC black teas.
研究领域:
农业科学
·
食品科学
·
化学
·
植物科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2015
·Vol. 15
·期 6
·4608-4618
·
IF 1.556
Cui Qinmin,Zhu Shudi,Yan Yingjie,Ye Quanlin,Ziener Ulrich,Cao Zhihai
In the present study, superparamagnetic Fe3O4/poly(N-isopropyl acrylamide) nanocomposites were synthesized by one-step inverse miniemulsion copolymerization of N-isopropyl acrylamide and N,N'-methylene diacrylamide. The loading of Fe3O4 nanoparticles
in the nanocomposites was 27 wt%, and the saturation moment of the nanocomposites was 12.4 emu · g−1. Fe3O4 nanoparticles were prepared through a coprecipitation method. The amount of stabilizer (poly(acrylic acid)) significantly influenced the
size and size distribution of the Fe3O4 nanoparticles, and, therefore, their magnetic properties. Superparamagnetism of the Fe3O4 nanoparticles was preserved in the nanocomposites. The effects of synthetic parameters on the particle properties, namely
surfactant loading, concentration of ferrofluid, type of lipophobe and initiator, and amount of cross-linker were investigated. Nanocomposites of Fe3O4/poly(N-isopropyl acrylamide) displayed a guava-like morphology, which they could retain after being redispersed
in polar solvents.
研究领域:
材料科学
·
纳米技术
·
化学工程
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Hengye Li,Xuemeng Zhang,Lin Zhang,Weihua Cheng,Fenying Kong,Dahe Fan,Lei Li,Wei Wang
4-Carboxy-benzoboroxole was designed and synthesized. It was then combined with the modification effect of polyethyleneimine (PEI) for the preparation of boronate affinity silica stationary phase. The stationary phase showed improved binding strength with dissociation constant ( K d ) towards xanthosine as low as 2.48 × 10 −4 M. The column showed excellent selectivity , high binding capacities (88.3 μmol adenosine g −1 , pH 7.0) and the lowest binding pH (4.0 for cytidine and as low as 2.24 for xanthosine). These binding properties were superior to the existing boronate affinity materials, facilitating the selective extraction of trace cis -diol compounds in complex samples and greatly expanding the application scope of boronate affinity chromatography . In addition, the column showed secondary separation capability under acidic conditions and this secondary separation capability was investigated thoroughly. It was found that the separation was pH-dependent and mainly determined by binding strength with the possibility of involvement of other interaction, providing alternative strategy for the separation of cis -diol compounds. The feasibility and practicability were demonstrated through the selective enrichment of nucleosides in urine samples and the results indicated the excellent performance and great potential for the extraction of trace cis -diol compounds in complex samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）