ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Zhichen Zhang,Yujing Zhu,Zejing Xing,Jing Li,Qin Xu,Jun-Jie Zhu,Cheng Ma
Electrochemiluminescence (ECL) has manifested a surface-confined emitting character and a low light background occurring near the electrode surface. However, the luminescence intensity and emitting layer are limited by the slow mass diffusion rate and electrode fouling in a stationary electrolyte. To address this problem, we developed an in situ strategy to flexibly regulate the ECL intensity and layer thickness by introducing an ultrasound (US) probe to the ECL detector and microscope. Herein, we explored the ECL responses and the thickness of ECL layer (TEL) under US irradiation in different ECL routes and systems. ECL microscopy with an ultrasonic probe discovered that ultrasonic radiation enhanced the ECL intensity under the catalytic route, while an opposite trend was observed under the oxidative-reduction route. Simulation results demonstrated that US promoted the direct electrochemical oxidation of TPrA radicals by the electrode rather than oxidant Ru(bpy)33+, which made the TEL thinner than that in the catalytic route under the same US condition. In situ US boosted the ECL signal from 1.2 times to 4.7 times by improving the mass transport and weakening electrode fouling due to the cavitation role. It significantly enhanced the ECL intensity beyond the diffusion-controlled ECL reaction rate. In addition, a synergistic sonochemical luminescence is validated in the luminol system to enhance the whole luminescence because cavitation bubbles of US promoted the generation of reactive oxygen species. This in situ US strategy provides a new opportunity to understand ECL mechanisms and a new tool in regulating TEL to meet the needs of ECL imaging.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Rongbin Nie,Huiyu He,Yiping Chen
Rapid and on-site detection of chloramphenicol (CAP) is of great significance for food safety. Herein, we established an affordable and portable homogeneous fiber optic chemiluminescent immunosensor (hoFOCI) by streamlining the immunoassay and integrating the detection platform. By immobilizing the artificial antigens of CAP and the luminescent functional groups onto the fiber optic, the biorecognition and signal conversion could be performed without any washing steps, which greatly reduced the time consumption and simplified the operation. For chemiluminescence signal detection, a low-cost and integrated detection platform was constructed, which was designed to be compatible with fiber optic probe . The hoFOCI had a good linear response to CAP in the concentration of 10–10 6 pg/mL, with a detection limit of 2.95 pg/mL. The feasibility was also validated by assaying CAP in real honey samples, and a high accuracy and reproducibility were obtained. Therefore, the proposed hoFOCI is expected to be appropriate for rapid and on-site detection of CAP in food stuffs.
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LUMINESCENCE
·
2019
·Vol. 34
·期 7
·673-679
·
IF 1.691
Min Zhou,Qiqi Chen,Ailian Wang,Juanhua Li,Yongjun Ma
The chemiluminescence (CL) behaviour of the luminol–potassium periodate system enhanced by CdTe quantum dots capped with thioglycolic acid (TGA–CdTe QDs) was studied using kinetic experiments, CL spectra, UV–vis absorption spectra and fluorescence spectra. The production of oxygen-containing reactant intermediates (O 2 •− and OH • ) in the present CL system was verified by CL. The possible CL mechanism was discussed in detail. Furthermore, theophylline (THP) was determined based on its enhancement of the CL intensity of the CdTe QDs–luminol–potassium periodate system coupled with a flow-injection technique. Under these optimized conditions, the linear range was found to be from 1.0 × 10 −8 to 1.0 × 10 −5 g/mL with a detection limit of 2.8 × 10 −9 g/mL (3σ). The recoveries for the determination of THP in tablets were from 98.2 to 99.6%.
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高影响因子
ELECTROANALYSIS
·
2019
·Vol. 31
·期 4
·624-631
·
IF 2.691
Yaru Yang,Yanhui Zhang,Lu Wei,Guixin Li,Ming Guan,Shuge Tian
Poly(aniline-luminol-hemin) nanocomposites are prepared on an electrode surface through electropolymerization, and a highly sensitive electrochemiluminescence (ECL) biosensor for choline is developed based on the poly(aniline-luminol-hemin) nanocomposites and an enzyme catalyzed reaction of choline oxidase (CHOD). The obtained nanocomposites are characterized by scanning electron microscopy (SEM), atomic absorption spectrometry (AAS) and ECL. The results indicate that hemin can be incorporated into the poly(aniline-luminol) nanocomposites using the facile electropolymerization method, and the poly(aniline-luminol-hemin) nanocomposites are rod shaped porous nanostructure. Moreover, the poly(aniline-luminol-hemin) nanocomposites exhibit higher ECL intensity than poly(aniline-luminol) nanocomposites in alkaline media due to the catalytic effect of hemin on the ECL of the polymerized luminol and the electron transfer ability of hemin in the nanocomposites. CHOD is immobilized on the surface of the poly(aniline-luminol-hemin) nanocomposites modified electrode with glutaraldehyde, and the ECL biosensor based on poly(aniline-luminol-hemin)/CHOD exhibits a wider linear range for the choline detection. The enhanced ECL signals are linear with the logarithm of concentration of choline over the range of 1.0×10 −11 ∼1.0×10 −7 mol L −1 with a low detection limit of 1.2×10 −12 mol L −1 . Moreover, the proposed biosensor is successfully applied to the detection of choline in milk.
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高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Bingru Liu,Xiaohua Li,Sumin Liu,Xu Hun
In this work, a chemiluminescence (CL) aptasensor was designed for detection of microcystin-RR (MC-RR) by introducing Cu/Co nanorods acting as CL catalyzer. Cu/Co nanorods were firstly synthesized throughout a hydrothermal method . Then the thiolated MC-RR aptamer was immobilized on the surface of Cu/Co nanorods, which was employed for recognizing of MC-RR target. In the presence of MC-RR, the conformational change of MC-RR aptamer was happened via recognizing between MC-RR and its aptamer. The luminol CL was catalyzed by Cu/Co nanorods that influenced by conformational change of MC-RR aptamer. The formed MC-RR/aptamer composite prevented the diffusion channel of luminol substrate toward the Cu/Co nanorods surface and restrained the Cu/Co nanorods catalyzing luminol CL. And the intensity of CL depended on the concentration of MC-RR. Under optimal condition, the CL aptasensor had a linear range in 0.1 to 70 nM and a low detection limit of 3.3 × 10 −11 M for MC-RR. This study provided a significant method for MC-RR detection in real samples with excellent selectivity.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-10
·
IF 5.705
Ma Xionghui,Li Shuhuai,Pang Chaohai,Xiong Yuhao,Li Jianping
The authors describe a method of electrochemiluminescent quantitation of the antibiotic sulfaquinoxaline (SQX). It relies on the use of a molecularly imprinted polymer and a Cu(II)-anchored unzipped covalent triazine framework (UnZ-CCTF) with excellent dispersibility, electrical conductivity, and peroxidaze-like activity. The framework was prepared by unzipping a covalent triazine framework under retention of basic triazine units. It was morphologically and structurally characterized by a range of instrumental techniques. The excellent peroxidase-mimicking effect of UnZ-CCTF on the electrochemiluminescence of the luminol/H 2 O 2 system was exploited to design an ultrasensitive SQX assay with a 1.0–20 pM detection range and a detection limit of 0.76 pM (at 3δ/m). The technique was used for SQX quantitation in spiked milk samples, achieving recoveries of 94.0–104.8%.
Scheme of the sulfaquinoxaline molecularly imprinted electrochemiluminescence sensor based on Cu-anchored unzipped covalent triazine frameworks.
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高影响因子
TALANTA
·
2018
·
IF 4.244
Xiao-Chong Tang,Yi Xiao,Ping Li,Lian-Wen Qi
Artemisinin is an important frontline antimalarial . Fast, accurate detection of artemisinin in human serum is of importance in monitoring its clinical pharmaceutical effect. In this work, a strategy using microsensor array coupled with electrochemiluminescence (ECL) imaging technique was developed for detection of artemisinin. The microsensor array was constructed by integrating a patterned indium tin oxide glass plate with two perforated hydrophobic paper covers. By introducing the reactant of p-aminophenylboronic acid, luminol and artemisinin into the microsensor array, artemisinin would oxidize p-aminophenylboronic acid into p-aminophenol, a product which can efficiently inhibit the ECL of luminol. ECL signals decrease linearly with the increase of artemisinin. Based on the decreased ECL signal, artemisinin can be accurately detected. A good linearity ( r = 0.994) was observed for artemisinin detection. The detection sensitivity is 0.48 μM for artemisinin. The detection selectivity and stability were also investigated. Results show that the present method shows a good selectivity and stability towards artemisinin detection. To evaluate the applicability of the present strategy for detecting artemisinin in real samples, the artemisinin content in human serum and Artemisia annua samples were analyzed. Results demonstrated that the present strategy shows excellent selectivity with high sensitivity towards artemisinin detection in real samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yonghong Wang,Zeyang Fang,Ge Ning,Shaoming Mao,Yaohui Wu,Shun Wu,Gao-Qiang Liu
After successively introducing elements of a triple-helix aptamer probe (TAP) and a luminol–hydrogen peroxide (H 2 O 2 ) solution, we proposed a brand-new chemiluminescence (CL)-based biosensor for the assay of ochratoxin A (OTA). Primarily, the TAP contains an aptamer loop, two DNA segment stems, and a triplex-forming oligonucleotide (signal probe). In the presence of a target, the aptamer sequence binds to the target with an impressive affinity. This association leads to a conformational change in the TAP, thus releasing the signal probe. Sequentially, with the participation of hemin and potassium ions, the dissociated probe forms into a G-quadruplex structure that is capable of catalyzing luminol to produce CL mediated by H 2 O 2 . In contrast, in the absence of the target, a low background signal remains. Using this approach of combining a novel TAP switch with a G-quadruplex-catalyzed luminol-H 2 O 2 system, we presented a label-free and signal-on CL strategy for the measurement of OTA. Under optimal conditions, the gradually strengthened signal maintained a linear dependence on target concentrations ranging from 0.1 ng/mL to 2.0 ng/mL, and the detection limit was as low as 0.07 ng/mL. This newly designed, simple, and cost-effective protocol affords high sensitivity, specificity, suitability, and efficacy for biosensing. Thus, this protocol is attractive for a range of target quantifications, with only the matched aptamer requiring replacement (no redesign of signal probes).
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BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Kaige Zhao,Min Tang,Huaisheng Wang,Zhenxian Zhou,Yafeng Wu,Songqin Liu
An immunosensing biochip for simultaneous detection of three biomarkers related to acute myocardial infarction (AMI) was developed based on anionic soybean peroxidase (SBP) functionalized nanoprobe and chemiluminescent imaging. The nanoprobes (Ab2-SiO 2 -SBP) were fabricated by co-immobilization of SBP and one of the detection polyclonal antibodies , anti-cardiac troponin I antigen (anti-cTnI), anti-creatine kinase-MB (anti-CK-MB) and anti-myoglobin (anti-Myo), on the silica nanoparticle surface. The detection sensitivity was enhanced since the large surface area of silica carriers increased the loading of SBP for per sandwiched immunoreaction. The immunosensing biochip designed as 3 × 8 wells array was constructed by simultaneously immobilizing three capture monoclonal antibodies on the same one microtiter well with 2 × 3 active spots. In the presence of target protein, the nanoprobes will be attached onto the spots with high specificity through the sandwiched immunoreactions, which triggered the chemiluminescence (CL) signals on each sensing site of the microtiter plates and allowed to CL imaging of three biomarkers in one well at the same time. Therefore, the proposed biochip was a promising convenient strategy for simultaneous detection of cTnI, CK-MB and Myo, which showed potential application for multianalyte determination in clinical diagnostics.
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高影响因子
ANALYST
·
2018
·Vol. 144
·期 2
·498-503
·
IF 3.864
Xiu-Xiu Zhang,Jia-Juan Liu,Yu Cai,Shuang Zhao,Zhi-Yong Wu
A sensitive detection method combined with an effective on-line concentration may improve the analytical performance of a paper-based analytical device (PAD), and its merits of low cost and portability in POCT are fully demonstrated. Here, a sensitive PAD system with chemiluminescence (CL) detection and electrokinetic preconcentration was introduced, and the performance was demonstrated by the detection of hemin. A commercially available low cost and miniaturized optical detection module was used for the CL detection. Firstly, hemin was stacked on a simple paper fluidic channel based on field amplified stacking (FAS), and then a CL reagent (luminol–H2O2) was loaded on the stacked band to initiate the CL reaction. The photons were directly detected using the detection module. With optimization of the background electrolyte (BGE), voltage and CL reagent, a limit of detection (LOD) of 0.58 nM for hemin was obtained with a linear range of 1–1000 nM (R2 = 0.995). With FAS, the signal intensity was about 13-fold enhanced. This PAD also exhibited satisfactory selectivity over possible interfering components at a 104 times higher concentration. The applicability of the PAD was demonstrated by the detection of hemin from iron supplements and human serum samples. With total manual operation, recovery rates of 84.8–115.6% were obtained with an RSD of less than 14.3%. With the introduction of the optical detection model, and together with FAS, both the LOD and dynamic range of this PAD were effectively improved.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-7
·
IF 5.479
Zhao Maoyu,Qi Wenjing,Fu Yuling,He Hongkun,Wu Di,Qi Lin,Li Rong
A method is presented for electrochemiluminescent (ECL) detection of the food additive curcumin via an energy transfer strategy and by using luminol-doped silica nanoparticles (luminol-NPs). The ECL emission of the luminol-NPs (peaking at 425 nm) is reduced in the presence of curcumin due to spectral overlap. The assay can be performed within 1 min, response is linear in the 0.1 to 100 µM curcumin concentration range, and the limit of detection is 32 nM. The method is selective over many ions, adenosine triphosphate, ascorbic acid, cysteine and folic acid. It was successfully applied to the determination of curcumin in spiked human serum and urine. The average recoveries range from 99.0 to 102.6%.
Electrochemiluminescence (ECL) “turn-off” detection of curcumin at levels as low as 32 nM via energy transfer using luminol-doped silica nanoparticles. No hydrogen peroxide (H 2 O 2 ) is used in ECL detection which makes the luminol-NPs ECL system more stable than the conventional luminol-H 2 O 2 ECL system.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Juan Li,Xu Zhao,Li-Jian Chen,Hai-Long Qian,Wen-Long Wang,Cheng Yang,Xiu-Ping Yan
Aflatoxin B1 (AFB1) contamination is one of the most critical global issues in food safety. The high carcinogenic nature necessitates rapid and specific methods for the determination of AFB1 in foodstuffs at ultratrace levels. Here, we report an enhanced bienzymatic chemiluminescence competitive immunoassay for ultrasensitive and high-throughput determination of AFB1. In this assay, protein G was first coated on the wells of a microplate for recognizing the Fc fragment of anti-AFB1 mAbs to reduce the antibody dosage and guarantee high immunological reaction efficiency. The target AFB1 competed with glucose oxidase labeled AFB1 for the limited anti-AFB1 mAbs in the wells of the microplate. p-Bromophenol was employed as an enhancer to obtain intense and long-lasting chemiluminescence. The utilization of an enhancer and bienzymatic catalysts effectively improved the detection sensitivity. The developed method offered a good linearity over 5 orders of magnitude, a detection limit of 5 pg L–1, and a relative standard deviation of 1.9% for AFB1. The application of the developed method to the analysis of grain samples gave quantitative recoveries from 94.0% to 97.0%. The developed method provides a universal platform for high-throughput, ultrasensitive, and high specific detection of pollutants or nutrients in foods.
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