PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·
IF 3.44
Bi Wang,Shu Xu,Yan Cao,Fei Liu,Xingzeng Zhao,Xu Feng
10-deacetyl-bacatin III (10-DAB) is a natural plant-derived taxane diterpene, whose antimicrobial activity against phytopathogens remains unknown. In this study, we demonstrated the antimicrobial effect of 10-DAB on plant-pathogenic oomycetes . Our results revealed that 10-DAB exhibited significant antimicrobial activities against test oomycetes, especially against Phytophthora capsici , with a median effective concentration (EC 50 ) of 1.46 μg/mL, but had no effect on test fungi. Under 10-DAB treatment, mycelia of P. capsici were contorted with an increased number of top branches, and the production and germination of zoospores were inhibited and delayed, respectively. In addition, 10-DAB had favorable protective and curative activities with control efficacies of 63.90% and 74.81% at 200 μg/mL on detached pepper leaves. Furthermore, 10-DAB caused a significant decrease in soluble protein , lysine, and α, ε-diaminopimelic acid content of P. capsici , which suggested that 10-DAB inhibited the lysine biosynthesis . On the contrary, treatment with exogenous lysine effectively counteracted 10-DAB's inhibition activity on P. capsici . Moreover, relative expression of four key lysine biosynthesis-related genes of P. capsici were decreased upon 10-DAB treatment. Taken together, our findings suggest a lysine biosynthesis inhibiting-dependent antimicrobial activity of 10-DAB against P. capsici , which contributes to accelerating the application of 10-DAB for successful management of phytophthora blight disease in agricultural production.
研究领域:
植物病理学
·
分子生物学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ORAL DISEASES
·
2023
·
IF 3.8
Sitong Hu,Yihui Zhu,Shujia Yu,Yanchuan Guo,Yihu Wang,Menghao Lv,Wei Bai,Pan Ma
Objective The aim of this study was to investigate the effect of IL-10 on the phenotype polarization of macrophages and osteogenesis in diabetes mellitus type 2 (T2DM) rat jaw defects. Methods Lipopolysaccharide (LPS) and interleukin-10 (IL-10) were chosen to induce the polarization of macrophages. In vitro assessment included wound-healing assay, western blotting, and alizarin red staining after co-culture of the bone marrow-derived mesenchymal stem cells (BMSCs) and induced macrophages. For in vivo study, IL-10 was loaded on GelMA-Heparin and applied to bone defects of the alveolar ridge in diabetic rats, while Bio-Oss Collagen, simple GelMA-Heparin, and blank control groups were set for contrast experiment. The mandibles of rats were processed for micro-computed tomography, histology, and immunohistochemistry 1 week and 4 weeks after the operation. Results IL-10 induced expression of arginase 1, TGF-β1, EGR2, and Mannose Receptor (CD206), whereas LPS induced expression of iNOS, TNF-α, IL-6, CD80. The BMSCs co-cultured with macrophages induced by IL-10 showed increased migration, osteogenic differentiation, and mineralization in vitro. Notably, the IL-10-laden GelMA-Heparin group showed quicker new bone formation and a higher M2/M1 ratio of macrophages in the jawbone defect area compared with the control groups. Conclusions IL-10 can stably induce macrophages to M2 type, thereby influencing BMSCs and improving the osteogenesis of jaw bone defects.
研究领域:
免疫学
·
骨科
·
再生医学
·
口腔科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2021
·
IF 7.561
Wu Binxin,Gao Furong,Lin Jianhua,Lu Lixia,Xu Huiming,Xu Guo-Tong
Allergic conjunctivitis (AC) is the most prevalent form of mucosal allergy, and the conditioned medium (CM) from mesenchymal stem cells has been reported to attenuate some allergic diseases. However, the therapeutic effects of CM from different tissue stem cells (TSC-CM) on allergic diseases have not been tested. Here, we studied the effects of topical administration of different human TSC-CM on experimental AC (EAC) mice. Only human amniotic epithelial cell-CM (AECM) significantly attenuated allergic eye symptoms and reduced the infiltration of immune cells and the levels of local inflammatory factors in the conjunctiva compared to EAC mice. In addition, AECM treatment decreased immunoglobulin E (IgE) release, histamine production, and the hyperpermeability of conjunctival vessels. Protein chip assays revealed that the levels of anti-inflammatory factors, interleukin-1 receptor antagonist (IL-1ra) and IL-10, were higher in AECM compared to other TSC-CM. Furthermore, the anti-allergic effects of AECM on EAC mice were abrogated when neutralized with IL-1ra or IL-10 antibody, and the similar phenomenon was for the activation and function of B cells and mast cells. Together, the present study demonstrated that AECM alleviates EAC symptoms by multiple anti-allergic mechanisms mainly via IL-1ra and IL-10. Such topical AECM therapy may represent a novel and feasible strategy for treating AC.
研究领域:
眼科学
·
免疫学
·
干细胞治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Qiulan Zhang,Linghong Liu,Yongnian Ni
10-hydroxycamptothecin (HCPT) is a natural alkaloid which has strong antitumor activity by inhibiting the degradation of the cleaved DNA strand. As a rich plasma protein, human serum albumin is a medium for transporting of various drugs and endogenous compounds. Due to its high water dispensability and good biocompatibility, reduced graphene oxide (rGO) is an eminent candidate for delivery of medicinal molecules. In cancer therapy, ferrosoferric oxide (Fe 3 O 4 ) could be used to improve the therapeutic action and the imaging-guided delivery. In this work, Fe 3 O 4 was anchored onto rGO nanosheets, and the formed Fe 3 O 4 /rGO nanocomposite showed efficient tumor targeting. The binding interaction between HCPT with HSA was investigated and the magnetic reduced graphene oxide (MrGO) had strong effect on the binding interaction. The comet assay results showed that MrGO had low toxicity, and the main binding forces between HCPT and HSA or HSA-HCPT were hydrogen bond and van der Waals force. In addition, it was found that the added MrGO greatly enhanced the binding of HCPT with HSA. The results offer some insight on the interaction between nanomaterials loaded with medicinal molecules and serum albumin at a molecular level.
研究领域:
药学
·
纳米医学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Lu Xiu,Rongguang Ge,Siyu Lu,Linwei Li,Wenqi Huang,Guojun Du,Zengli Zhang,Jie Zhang,Zhongxiao Wan,Jie Chang
Diabetes, a ruthless chronic metabolic disorder, is increasingly considered to jeopardize global well-being over the decades. Royal jelly (RJ) is a complex natural product secreted by worker bees containing a unique fatty acid known as 10-hydroxy-2-decenoic acid (10-HDA). This study aimed to verify the protective effect on cognitive function of RJ and 10-HDA in diabetic mice and explore the underlying mechanism. Briefly, our animal experiment results suggested that 10-HDA had a significant hypoglycemic effect and improved cognitive performance. Through western blotting, RJ and 10-HDA intervention notably increased Atg7, Atg16L1, Beclin-1, Atg5, Atg12, Atg3, and LC3-II expression and decreased p62 expression. Meanwhile, decreased mTOR expression and increased ULK1 expression were also observed in the 10-HDA intervention group. In conclusion, 10-HDA can regulate mTOR/ULK1 pathway to activate autophagy to alleviate hippocampal damage, thereby improving cognitive function in mice with diabetes, which may provide a new option for real-world cognitive impairment management.
研究领域:
医学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 11
·2478-2489
·
IF 7
Yanhao Zhang,Ji Wang,Chao Liu,Hanlei Xing,Yuhao Jiang,Xinsong Li
7-Ethyl-10-hydroxyl camptothecin (SN38), a semisynthetic derivative of camptothecin, exhibited extreme pharmacological activities in treating a range of cancers. However, its poor aqueous solubility and low stability hinder its clinical applications. Hence, a redox-responsive SN38 prodrug encapsulated human serum albumin (HSA) nanoparticle is developed to realize its potential in the clinic. First, a disulfide bond bridged 7-ethyl-10-hydroxyl camptothecin-undecanoic acid conjugate (SN38-SS-COOH) was synthesized and characterized structurally. After that, SN38-SS-COOH/HSA nanoparticles (SNH NPs) were prepared by the desolvation method. The SNH NPs with a feed molar ratio of 9 : 1 of SN38-SS-COOH : HSA showed a spherical structure with a diameter range of approximately 120–150 nm revealed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Fluorescence quenching confirmed the formation of SNH NP complexes by dual hydrophobic force and electrostatic interaction. The SNH NPs have a high drug loading of 10.44% and an encapsulation efficiency of 89.59% with good stability. Moreover, the redox responsiveness was validated by glutathione (GSH)-triggered accelerated release of parent drug SN38. In an in vivo pharmacokinetic study, the SNH NPs exhibited a significantly prolonged circulation time (t1/2, 3.77-fold) compared with free SN38. Finally, the in vivo antitumor efficacy and systemic toxicity of SNH NPs in a breast xenograft model were thoroughly evaluated. The inhibition rate of tumor growth induced by the SNH NPs reached 70.1%, while only 50.1% was achieved for irinotecan at an equivalent SN38 dosage of 10 mg kg−1. More importantly, the SNH NPs achieved a higher level of tumor growth inhibition (85.3%) by increasing the dosage to 60 mg kg−1 SN38 without obvious adverse effects. Taken together, the use of redox-responsive SN38 prodrug/HSA NPs could be a promising strategy to deliver highly active SN38 for breast cancer chemotherapy.
研究领域:
药学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Hongzhan Liu,Fangshuo Liu,Kaixiang Ji,Yuriy T. Zholudov,Islam M. Mostafa,Baohua Lou,Wei Zhang,Guobao Xu
9-Mesityl-10-methylacridinium ion (Acr+-Mes) is a donor–acceptor molecule with a much longer lifetime and a higher energy electron transfer excited state than natural photosynthetic reaction centers. Unlike lucigenin with a coplanar geometry, Acr+-Mes has an orthogonal geometry. There is no π conjugation between Acr+ and Mes. Due to its special electron donor–acceptor structure, it does not rely on strong alkalinity to generate an electron transfer state like lucigenin, which makes it possible to achieve chemiluminescence (CL) under weakly alkaline or neutral conditions. In this study, we report Acr+-Mes CL for the first time. Acr+-Mes generates about 400 times stronger CL intensity than lucigenin under neutral conditions (pH = 7) using KHSO5 as the coreactant. Moreover, Co2+ can enhance Acr+-Mes/KHSO5 CL remarkably. Acr+-Mes/KHSO5 CL enables Co2+ detection with a linear range of 0.5–500 nM and a limit of detection of 28 pM (S/N = 3). This method was tested for the detection of Co2+ in lake water, and the standard recovery rate of 96.8–107% was achieved. This study provides a new way to develop efficient CL systems in neutral solutions.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Mei Xiang,Jingna Liu,Wenqian Fu,Tiandi Tang,Dongfang Wu
A hierarchically porous ETS-10-supported highly dispersed Ni catalyst was designed to selectively catalyze the conversion of cellulose to levulinic acid under mild reaction conditions. Full conversion of cellulose and remarkably high yield (91.0%) of levulinic acid are achieved due to the synergistic effect of the unique porous structures and Lewis acid sites in the presence of a hydrogen atmosphere. Moderate Lewis acidic centers of the heterogeneous catalyst are revealed to play a positive role in activating the reaction substrates and intermediates, resulting in prominently catalytic activity. Meanwhile, hierarchical pores centered at 16 nm in ETS-10 benefit the mass transfer and therefore further enhance its catalytic performance. Also, during the process of levulinic acid formation, introduction of hydrogen is responsible for converting cellulose thoroughly and keeping the metal Ni from oxidization by the oxy-compounds, which is distinctive from the widely reported pathway dominated only by cellulose hydrolysis.
研究领域:
催化
·
化学工程
·
材料科学
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Can Wu,Ruixin Li,Yajun Yin,Junling Wang,Li Zhang,Wenying Zhong
A redox-responsive supramolecular hydrogel system was developed for delivering 10-hydroxy camptothecin (HCPT). The hydrogel was formed by cleaving disulfide bond. The combination of hydrophobic HCPT with hydrogel was a simple and effective way to improve the solubility of HCPT and the drug loading capacity of delivery system. The transmission electron microscopy (TEM) image revealed the self-assembled hydrogel was long and thin nanofibers with a width of < 10 nm. Rheological test verified the hydrogel had fine physical properties. In vitro release experiment showed that the accumulative releasing percentages within 72 h of HCPT-peptide hydrogels at 3.0%, 4.0%, 5.0% were 16.8%, 21.3%, and 26.8% respectively, which indicated the HCPT-peptide hydrogels had a significantly sustained-release characteristic. Besides, in vitro anticancer assay showed that HCPT-peptide hydrogels possessed a favorable anticancer efficacy. These results indicated that HCPT-peptide hydrogel had great potential for cancer treatment as a novel injectable drug delivery system.
研究领域:
药学
·
生物材料
·
肿瘤学
✓ 已验证
DOI
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2020
·Vol. 306
·期 1
·2000506
·
IF 3.853
Zhaojie Yang,Minghua Zhang,Yafei Xiao,Xi Zhang,Minmin Fan
Anion exchange membranes (AEMs) are one of the core components of AEM fuel cells. A series of poly(vinyl alcohol)/polyquaternium-10 (PVA/PQ-10) AEMs with semi-interpenetrating networks (s-IPNs) are prepared by a simple solution-casting method using glutaraldehyde (GA) as a cross-linking agent. Subsequently, the prepared PVA/PQ-10 cross-linked membranes are characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, mechanical analysis, water uptake and swelling ratio tests, ion exchange capacity (IEC) tests, ionic conductivity measurements, and oxidative/alkaline stability tests. The effects of the mass ratio of PVA and PQ-10 and the amount of cross-linking agent GA on the performance of the PVA/PQ-10 cross-linked membranes are systematically explored. The results show that the cross-linked PVA/PQ-10 AEMs have high IEC and low water uptake and swelling ratio, and its maximum ionic conductivity can reach 79.37 mS cm –1 at 80 °C. In addition, the PVA/PQ-10 cross-linked membrane has good oxidative and alkaline stability under optimal preparation conditions. These results may provide valuable insights toward more effective scheme designs and new, simple preparation methods for AEMs with s-IPN structures.
研究领域:
材料科学
·
化学工程
·
能源材料
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
HYDROMETALLURGY
·
2025
·
IF 5.3
Haokai Peng,Zuoying Cao,Shengxi Wu,Qinggang Li,Mingyu Wang,Xinsheng Wu,Wenjuan Guan,Guiqing Zhang
As the demand for Ni continues to rise, the recovery of Ni from electroplating sludge leachate has become an effective approach to alleviate supply constraints. However, the presence of impurities such as Cu, Zn, and Ca in the leachate complicates the Ni recovery process. To address these challenges, a novel pyridine group extractant, 2-((dodecylthio) methyl) pyridine (DMPY), was designed, synthesized, and evaluated for its synergistic performance with Versatic 10 (C 10 H 20 O 2 ) in selectively extracting Ni. Under optimal conditions, Ni was selectively recovered from electroplating sludge leachate including Cu, Zn, and Ca through three steps of the extraction processes. Mechanistic studies revealed that both DMPY and Versatic 10 anion coordinated with Ni 2+ , providing charge balance, forming the complex [Ni 2+ (DMPY) 2 (C₁₀H 19 O₂ − ) 2 ]. This synergistic solvent system significantly improved Ni extraction efficiency while minimizing reagent consumption. In conclusion, The DMPY-Versatic 10 system offers a sustainable and environmentally friendly solution, enhancing the hydrometallurgical treatment of polymetallic electroplating sludge and contributing to resource recovery and environmental protection.
研究领域:
湿法冶金
·
环境工程
·
化学分离
·
资源回收
·
可持续化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jiang Chang,Deying Zou,Honglin Ren,Xilin Liu,Meng Li,Zhaozhao Si,Cheng Han,Zengshan Liu,Shiying Lu,Pan Hu
The 10-kDa chemokine interferon-gamma-inducible protein 10 (IP-10) is considered one of the most promising biomarkers for diagnosing both tuberculosis and COVID-19 infections. The blood samples of patients at different disease states contain different levels of IP-10, which need to be detected in a rapid, specific and ultrasensitive manner. Here, we report a bienzymatic chemiluminescence sandwich immunoassay (BCSI) assay for the ultrasensitive and stable detection of IP-10. In this assay, IP-10 is first efficiently captured using a double-antibody sandwich strategy. The detection antibody is linked to catalase (CAT) via a streptavidin–biotin signal amplification system to achieve highly efficient conversion of hydrogen peroxide (H 2 O 2 ) to oxygen and water. In the chemiluminescence (CL) reaction, horseradish peroxidase (HRP) acts as an efficient catalyst, and 4-bromophenol acts as an enhancer for the cyclic transition of HRP, which results in a strong and durable CL signal. The bienzymatic catalysis with CAT and HRP and the potentiation of 4-bromophenol enables the assay to be ultrasensitive and stable. The CL intensity was found to be well correlated with the detection of IP-10 at levels in the range of 0.71 to 125,000 pg/mL, which covers more than 6 orders of magnitude, with a detection limit of 0.63 pg/mL. The coefficient of variation was 1.49%, and the recovery range of IP-10 in serum was 86.21%-104.57%. This assay provides a wide linear range and high sensitivity and may be a promising method for the high-throughput detection of IP-10 in the diagnosis of tuberculosis and COVID-19.
研究领域:
医学诊断
·
免疫学
·
化学生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）