Nature Energy
·
2024
·
IF 49.7
Shi Jiangjian,Wang Jinlin,Meng Fanqi,Zhou Jiazheng,Xu Xiao,Yin Kang,Lou Licheng,Jiao Menghan,Zhang Bowen,Wu Huijue,Luo Yanhong,Li Dongmei,Meng Qingbo
Kesterite Cu 2 ZnSn(S, Se) 4 (CZTSSe) solar cells are highly promising low-cost thin-film photovoltaics. However, the efficiency of these solar cells is challenged by severe charge losses and complex defects. Here we reveal through a data-driven correlation analysis that the dominant deep defect in CZTSSe exhibits a donor character. We further propose that incomplete cation exchange in the multi-step crystallization reactions of CZTSSe is the kinetic mechanism responsible for the defect formation. To facilitate the cation exchange, we introduce a multi-elemental alloying approach aimed at weakening the metal–chalcogen bond strength and the stability of intermediate phases. This strategy leads to a significant reduction in charge losses within the CZTSSe absorber and to a total-area cell efficiency of 14.6% (certified at 14.2%). Overall, these results not only present a significant advancement for kesterite solar cells but could also help identify and regulate defects in photovoltaic materials.
研究领域:
材料科学
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光伏技术
·
可再生能源
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2026
·
IF 5.2
Bin Ou,Xiaofang Li,Lemei Huang,Yujiang Ke,Chaohui Che,Yanyan Huang,Zhijia Wang,Xiaobing Liu,Yajun Li,Kui Wu
The solubility of 3,3′-Diindolylmethane in 14 different solvents (272.45–324.25 K) was determined using the gravimetric method. The results show that its solubility increases with rising temperature across the studied solvent range. At 298.15 K, the solubility of 3,3′-Diindolylmethane in the solvents follows the order: 2-pentanone > ethyl acetate > methyl acetate > ethyl formate > acetonitrile > n-pentanol > n-butanol > n-propanol > ethanol > isopropanol > isopentanol > methanol > isobutanol > toluene. Five thermodynamic models were employed to correlate the experimental data, among which the modified Apelblat model provided the best fit. In addition, electrostatic potential energy surface analysis was conducted to preliminarily evaluate possible solute solvent interactions. Results from density functional theory calculations and the KAT-LSER model indicate that 3,3′-Diindolylmethane primarily acts as a hydrogen-bond donor, and its solubility is synergistically influenced by the hydrogen-bond acceptor ability, polarity, and hydrophobicity of the solvents. Furthermore, the mixing thermodynamic properties of the dissolution process were calculated using a Wilson-based model, revealing that the dissolution of 3,3′-Diindolylmethane in the studied solvents is a spontaneous, endothermic, and entropy-driven process.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Ying Li,Jing Jiang,Qingyi Lv,Bohuai Shao,Chuang Wang,Ge Zhu
The discovery of high color purity red-emitting phosphors is a major challenge for solid-state lighting materials. Benefitting from highly condensed and flexible framework structure of β -Ca 3 (PO 4 ) 2 -type compounds, we have successfully prepared three different kinds of novel high color purity red-emitting phosphors Sr 19 Mg 2 (PO 4 ) 14 : Re 3+ (Re 3+ = Eu 3+ , Sm 3+ , Pr 3+ ) by using traditional sintering method. Rietveld refinement, SEM measurement, absorption spectra, emission/excitation spectra, fluorescence decay analysis and emission spectra in terms of different temperature were investigated and discussed clearly. The matrix optical band gap was calculated to be 4.5 eV by reflection data, which indicated the suitable host for rare earth doping. The single doped Eu 3+ , Sm 3+ and Pr 3+ phosphors could respectively exhibit characteristic and strong red emission peaks at 614 nm, 598 nm and 642 nm when excited by (near) ultraviolet radiation. Excitingly, all samples could obtain high color purity with the value of 91.6%, 90.6% and 84.8% for Eu 3+ , Sm 3+ , Pr 3+ ions, respectively. Moreover, the thermal stability can stay strong which still keep over 75% at 150℃ when comparing with that at atmospheric temperature. The quantum efficiency (QE) is another important parameter for phosphors which were measured to be 46.6% for Eu 3+ , 53.1% for Sm 3+ and 10.3% for Pr 3+ . The present work indicates that the Sr 19 Mg 2 (PO 4 ) 14 : Re 3+ phosphors are efficient red components with extraordinary color purity and high quantum efficiency for industrial applications as solid-state lighting materials.
研究领域:
材料科学
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光学材料
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固态化学
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发光学
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磷光体技术
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Long Wang,Jingping Sun,Yuwei Hua,Li Xu,Guoji Liu
The solubility of 2-cyanoacetamide in N,N-dimethylformamide, acetone, acetonitrile, methanol, methyl acetate, tetrahydrofuran, ethanol, ethyl acetate, n-propanol, n-butanol, trichloromethane, dichloromethane, 1,4-dioxane, and water was measured by a laser dynamic method under atmospheric pressure in the temperature range of (273.15–318.15) K. The solubility order of 2-cyanoacetamide in selected solvents was N,N-dimethylformamide > acetone > water > acetonitrile > methanol > methyl acetate >1,4-dioxane > tetrahydrofuran > ethanol > ethyl acetate > n-propanol > n-butanol > trichloromethane > dichloromethane. Hansen solubility parameters were used to explain and predict the solubility behavior of 2-cyanoacetamide. The modified Apelblat equation, λh equation, NRTL model equation, and Wilson model were used to correlate the experimental solubility data. The values of the RMSD (less than 0.005) and RAD (less than 0.2) indicated that the modified Apelblat equation agreed well with the experimental values, compared to other models. The values of ΔmixG, ΔmixS, ΔmixH, the activity coefficient, and the reduced excess enthalpy were obtained on the basis of the Wilson model. The mixing process of 2-cyanoacetamide in the studied solvents was a favorable and spontaneous process. The and values were not similar, which means that 2-cyanoacetamide in selected solvent solution systems was nonideal.
研究领域:
物理化学
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材料科学
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Xi-Ya Liu,Hui-Mei Sun,Yi-Hong Luo,Mao-Ye Li,Hai-Bing Liu,Su Liu
In insects, glutathione S -transferases (GSTs) play a pivotal role in the detoxification of a wide range of pesticides. The cigarette beetle, Lasioderma serricorne , is an economically important pest insect of stored products. Recently, pyrethroid insecticides have been used to control this pest. However, little is known concerning the responses and functions of GSTs in L. serricorne under pyrethroid exposure. In this study, transcriptome sequencing was performed on L. serricorne , and a total of 14 GSTs were identified by retrieving the unigene dataset. Of these, 13 predicted GSTs fell into six cytosolic classes, namely, delta, epsilon, omega, sigma, theta, and zeta, and one was assigned to an “unclassified” group. The GST genes were differentially expressed in various larval tissues and at different developmental stages. Exposure to the pyrethroid insecticide lambda-cyhalothrin (LCT) caused oxidative stress in L. serricorne larvae and led to significantly elevated expression levels of six genes, among which LsGSTe1 was the most upregulated. Recombinant LsGSTE1 protein displayed LCT-metabolizing activity. Furthermore, LsGSTE1 protects cells against oxidative stress. Moreover, knockdown of LsGSTe1 by RNA interference dramatically increased the susceptibility of L. serricorne larvae to LCT treatment. The results from this study provide sequence resources and expression data for GST genes in L. serricorne . Our findings indicate that LsGSTE1 plays a dual role in LCT detoxification by metabolizing the pesticide and by preventing LCT-induced oxidative stress. Thus, the LsGSTe1 gene could be used as a potential target for sustainable management of the cigarette beetle.
研究领域:
昆虫学
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分子生物学
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农药科学
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基因组学
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION
·
2014
·Vol. 30
·期 3
·199-203
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IF 0.827
Wei-Bin Ma,Jun-Tao Feng,Zhi-Li Jiang,Xing Zhang
We studied the knockdown activity and lethal toxicity of 6 essential oil compounds—methyl salicylate, linalool, 2-phenethyl alcohol, eugenol, β-citronellol, and trans-cinnamaldehyde—as fumigants against adult female Culex pipiens pallens in the laboratory. Of the 6 products tested, trans-cinnamaldehyde was the most toxic (LC50 = 0.26 µl/l air, 24 h) with a slow knockdown time (KT95 = 176.5 min at 0.5 µl/l air). Methyl salicylate displayed a lower toxicity (LC50 = 1.17 µl/l air, 24 h) but the fastest knockdown activity (KT95 = 16.8 min) at the sublethal concentration 0.5 µl/l air. Furthermore, the binary mixture of methyl salicylate and trans-cinnamaldehyde exhibited a combined effect of fast knockdown activity and high toxicity against Cx. p. pallens adults, showing potential for development as natural fumigants for mosquito control.
研究领域:
昆虫学
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公共卫生学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR ENDOCRINOLOGY
·
2015
·Vol. 55
·期 2
·119-31
·
IF 3.081
Leping Zhao,Yong Pan,Kesong Peng,Zhe Wang,Jieli Li,Dan Li,Chao Tong,Yi Wang,Guang Liang
11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) controls the production of active glucocorticoid (GC) and has been proposed as a new target for the treatment of type 2 diabetes. We have previously reported that a natural product, curcumin, exhibited moderate inhibition and selectivity on 11β-HSD1. By analyzing the models of protein, microsome, cells and GCs-induced mice in vitro and in vivo, this study presented a novel curcumin analog, LG13, as a potent selective 11β-HSD1 inhibitor. In vivo, Type 2 diabetic mice were treated with LG13 for 42 days to assess the pharmacological benefits of 11β-HSD1 inhibitor on hepatic glucose metabolism. In vitro studies revealed that LG13 selectively inhibited 11β-HSD1 with IC50 values at nanomolar level and high selectivity over 11β-HSD2. Targeting 11β-HSD1, LG13 could inhibit prednisone-induced adverse changes in mice, but had no effects on dexamethasone-induced ones. Further, the 11β-HSD1 inhibitors also suppressed 11β-HSD1 and GR expression, indicating a possible positive feedback system in the 11β-HSD1/GR cycle. In type 2 diabetic mice induced by high fat diet plus low-dosage STZ injection, oral administration with LG13 for 6 weeks significantly decreased fasting blood glucose, hepatic glucose metabolism, structural disorders, and lipid deposits. LG13 exhibited better pharmacological effects in vivo than insulin sensitizer pioglitazone and potential 11β-HSD1 inhibitor PF-915275. These pharmacological and mechanistic insights on LG13 also provide us novel agents, leading structures, and strategy for the development of 11β-HSD1 inhibitors treating metabolic syndromes.
研究领域:
药理学
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内分泌学
·
代谢紊乱
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DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
·
2019
·Vol. 98
·期 3
·817-821
·
IF 1.201
Wang Wenchang,Chen Ye,Zhang Jing,Wang Xue,Chen Zhidong
A sensitive electrochemical method was developed for the simultaneous determination of Brilliant Blue (BB) and tartrazine (Tz) using an ionic liquid-modified expanded graphite paste electrode (IL-EGPE). The IL-EGPE was prepared by mixing ionic liquid-expanded graphite composite (IL-EG) with solid paraffin. Compared with the EGPE, the IL-EGPE remarkably enhanced the electrocatalytic oxidation signals of BB and Tz. Under optimal experimental conditions, the designed IL-EGPE exhibited wide linear responses to BB and Tz ranging from 5.0 × 10–9 to 4.0 × 10–6 M and 1.0 × 10–8 to 1.0 × 10–6 M, respectively. The detection limits for BB and Tz were 2.0 × 10–9 M (1.6 ng/mL) and 3.3 × 10–9 M (1.8 ng/mL) at an S/N of 3, respectively. This electrode showed good reproducibility, stability, and reusability. The proposed method was successfully applied in the simultaneous determination of BB and Tz in a soft drink with satisfactory results.
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DOI
高影响因子
JOURNAL OF VETERINARY MEDICAL SCIENCE
·
2015
·
IF 0.782
Yao ZOU,Xiaoming ZHU,Hassan Mushtaq MUHAMMAD,Ping JIANG,Yufeng LI
Recently, a series of acute swine erysipelas outbreaks occurred in Eastern China. Eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. One isolate strain named HX130709 was attenuated on agar media containing acriflavine dye. The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced. It was further compared with the vaccine strain G4T10, and thus, the eight field strains can be divided into four spaA-types. The partial spaA gene analysis also showed that no point mutations occurred among different archived passages of HX130709 during the attenuation. Results of pulsed-field gel electrophoresis showed that eight distinct patterns with 22 to 30 DNA fragment bands were produced from field strains, and twelve distinct patterns with 23 to 27 DNA fragment bands were produced from different passages of the attenuated strains. Mouse pathogenicity test showed that the mortality of the mice infected with 104 CFU field strains was 100% and the attenuation of strain HX130709 occurred between 46 and 50 passages. All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides. So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.
研究领域:
兽医学
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微生物学
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DOI
PubMed
高影响因子
mBio
·
2014
·Vol. 5
·期 3
·
IF 6.875
Boyu Cui,Yao Wang,Yunhong Song,Tietao Wang,Changfu Li,Yahong Wei,Zhao-Qing Luo,Xihui Shen
Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions. However, few of these methods allow the detection of dynamic and real-time protein-protein interactions in bacteria. Here we describe a bioluminescence resonance energy transfer (BRET) system based on the bacterial luciferase LuxAB. We found that enhanced yellow fluorescent protein (eYFP) accepts the emission from LuxAB and emits yellow fluorescence. Importantly, BRET occurred when LuxAB and eYFP were fused, respectively, to the interacting protein pair FlgM and FliA. Furthermore, we observed sirolimus (i.e., rapamycin)-inducible interactions between FRB and FKBP12 and a dose-dependent abolishment of such interactions by FK506, the ligand of FKBP12. Using this system, we showed that osmotic stress or low pH efficiently induced multimerization of the regulatory protein OmpR and that the multimerization induced by low pH can be reversed by a neutralizing agent, further indicating the usefulness of this system in the measurement of dynamic interactions. This method can be adapted to analyze dynamic protein-protein interactions and the importance of such interactions in bacterial processes such as development and pathogenicity.IMPORTANCE Real-time measurement of protein-protein interactions in prokaryotes is highly desirable for determining the roles of protein complex in the development or virulence of bacteria, but methods that allow such measurement are not available. Here we describe the development of a bioluminescence resonance energy transfer (BRET) technology that meets this need. The use of endogenous excitation light in this strategy circumvents the requirement for the sophisticated instrument demanded by standard fluorescence resonance energy transfer (FRET). Furthermore, because the LuxAB substrate decanal is membrane permeable, the assay can be performed without lysing the bacterial cells, thus allowing the detection of protein-protein interactions in live bacterial cells. This BRET system added another useful tool to address important questions in microbiological studies.
研究领域:
微生物学
·
分子生物学
·
细胞生物学
·
生物技术
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DOI
PubMed
高影响因子
EUKARYOTIC CELL
·
2015
·Vol. 14
·期 4
·
IF 2.82
Yanfang Shang,Peilin Chen,Yixiong Chen,Yuzhen Lu,Chengshu Wang
Two-component signaling pathways generally include sensor histidine kinases and response regulators. We identified an ortholog of the response regulator protein Skn7 in the insect-pathogenic fungus Metarhizium robertsii, which we named MrSkn7. Gene deletion assays and functional characterizations indicated that MrSkn7 functions as a transcription factor. The MrSkn7 null mutant of M. robertsii lost the ability to sporulate and had defects in cell wall biosynthesis but was not sensitive to oxidative and osmotic stresses compared to the wild type. However, the mutant was able to produce spores under salt stress. Insect bioassays using these spores showed that the virulence of the mutant was significantly impaired compared to that of the wild type due to the failures to form the infection structure appressorium and evade host immunity. In particular, deletion of MrSkn7 triggered cell autolysis with typical features such as cell vacuolization, downregulation of repressor genes, and upregulation of autolysis-related genes such as extracellular chitinases and proteases. Promoter binding assays confirmed that MrSkn7 could directly or indirectly control different putative target genes. Taken together, the results of this study help us understand the functional divergence of Skn7 orthologs as well as the mechanisms underlying the development and control of virulence in insect-pathogenic fungi.
研究领域:
真菌学
·
分子生物学
·
真菌遗传学
·
昆虫病理学
·
微生物致病机制
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DOI
高影响因子
JOURNAL OF AOAC INTERNATIONAL
·
2019
·Vol. 98
·期 1
·225-229
·
IF 1.201
Zhang Jing,Yong Xiaojing,Zhao Dongyan,Shi Qiuyi
The content of mononuclear Al (Ala%) changed with its determination time (ta) under different dosages of Ferron (7-iodo-8-hydroxyquinoline-5-sulfonic acid, [Ferron]), and the change of Ala% with [Ferron] at different ta was systematically investigated for the firsttime. Thus, the most appropriate ta was found with the optimal [Ferron]. Also, the judgment of the platform (flat or level portion) of the complete reaction on the absorption-time curve determined in the hydroxyl polyaluminum solution by Ferron timed spectrophotometry (Ferron assay) was first digitized. The time point (tb) of complete reaction between the medium polyaluminum (Alb)and Ferron reagent depended on the reaction extent, and time could not be used only to judge. Thus, the tb was accurately determined and reduced
to half of original, which improved the experiment efficiency significantly. The Ferron assay was completely optimized.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）