ACS Agricultural Science & Technology
·
2025
·
IF 2.9
Qianqi Zheng,Jingyu Zhao,Keai Fang,Bing Luo,Xinru Yi,Hui Li,Wancheng Hu,Hao Yi,Kailin Liu,Bei Yan
To combat fungal plant diseases and induce plant resistance, this study employed w/o/w double emulsion technology to fabricate composite microcapsules using poly(lactide-co-glycolide) and hydroxypropyl methylcellulose phthalate as wall materials, coencapsulating pyraclostrobin of the outer layer and plant hormone 24-epibrassinolide of the inner layer. The microcapsules exhibited an average particle size of 2.12 μm, pyraclostrobin encapsulation efficiency (EE) of 74.1%, and 24-epibrassinolide EE of 64.8%. Cumulative release profiles showed 62% pyraclostrobin release at 10 h, reaching a maximum of 82% by 72 h, while 24-epibrassinolide achieved 52% release at 10 h and 60% by 48 h. Compared with the technical pyraclostrobin, the microcapsules significantly enhanced the photostability of 24-epibrassinolide by 4.36-fold, thereby facilitating the long-term utilization of active ingredients. The microcapsule formulations exhibited superior control efficacy against rice leaf blast and significantly reduced acute toxicity to zebrafish (20-fold higher LC50 than technical).
研究领域:
农业化学
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植物保护
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药物递送系统
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环境毒理学
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纳米技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 1
·312
·
IF 4.9
Rui Zhang,Siyuan Peng,Xuejuan Zhang,Zhengwei Huang,Xin Pan
Objective: The objective of this study was to explore the possibility of treating heart failure in rats by delivering mRNA of 24-dehydrocholesterol reductase (DHCR24) into the body through lipid nanoparticles (LNPs). Methods: We established a heart failure rat model using doxorubicin. The experiment was divided into blank, model, mRNA stock solution cardiac injection, mRNA stock solution intravenous injection, LNP-mRNA stock solution cardiac injection, and LNP-mRNA stock solution intravenous injection groups. We directly injected DHCR24-mRNA or LNP-DHCR24-mRNA into the myocardium in three regions through an insulin needle passing through the intercostal space under the guidance of B-ultrasound. We recorded the mortality rate, body weight, 6-min walk test return times, and organ weight of rats after administration and detected the cardiac structure and function using B-ultrasound and transmission electron microscopy (TEM). Additionally, we tested for HE staining; PRDX2, Sirt3, and TRX1 protein expression; and IL-1 β, IL-10, VEGF, NT proBNP, and BNP cytokine concentrations. Results: Compared with the model group, the administration of DHCR24-mRNA significantly reduced mortality; decreased weight loss, the ratio of heart to tibia length, and spleen weight; and improved rat motility. The administration of DHCR24-mRNA can postpone the pathological morphological alterations of myocardial cells and reduce inflammatory infiltration. In terms of biochemistry, the administration of DHCR24-mRNA can increase the expression of the PRDX2, Sirt3, and TRX1 proteins; increase the concentrations of IL-10 and VEGF; and reduce the concentrations of IL-1β, NT proBNP, and BNP. The administration of DHCR24-mRNA can also delay the process of heart failure. The delivery and therapeutic effect of DHCR24-mRNA encapsulated in LNPs were better when compared to the other groups. Conclusions: DHCR24-mRNA encapsulated in LNPs can be effectively administered to rats with heart failure and exhibits some curative effects.
研究领域:
心血管病学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
Microbial Cell Factories
·
2017
·Vol. 16
·期 1
·1-10
·
IF 3.681
Xiong Liang-Bin,Liu Hao-Hao,Xu Li-Qin,Sun Wan-Ju,Wang Feng-Qing,Wei Dong-Zhi
Background The strategy of modifying the sterol catabolism pathway in mycobacteria has been adopted to produce steroidal pharmaceutical intermediates, such as 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), which is used to synthesize various steroids in the industry. However, the productivity is not desirable due to some inherent problems, including the unsatisfactory uptake rate and the low metabolic efficiency of sterols. The compact cell envelope of mycobacteria is a main barrier for the uptake of sterols. In this study, a combined strategy of improving the cell envelope permeability as well as the intracellular sterol metabolism efficiency was investigated to increase the productivity of 4-HBC.Results
MmpL3, encoding a transmembrane transporter of trehalose monomycolate, is an important gene influencing the assembly of mycobacterial cell envelope. The disruption of mmpL3 in Mycobacterium neoaurum ATCC 25795 significantly enhanced the cell permeability by 23.4% and the consumption capacity of sterols by 15.6%. Therefore, the inactivation of mmpL3 was performed in a 4-HBC-producing strain derived from the wild type M. neoaurum and the 4-HBC production in the engineered strain was increased by 24.7%. Subsequently, to enhance the metabolic efficiency of sterols, four key genes, choM1, choM2, cyp125, and fadA5, involved in the sterol conversion pathway were individually overexpressed in the engineered mmpL3-deficient strain. The production of 4-HBC displayed the increases of 18.5, 8.9, 14.5, and 12.1%, respectively. Then, the more efficient genes (choM1, cyp125, and fadA5) were co-overexpressed in the engineered mmpL3-deficient strain, and the productivity of 4-HBC was ultimately increased by 20.3% (0.0633 g/L/h, 7.59 g/L 4-HBC from 20 g/L phytosterol) compared with its original productivity (0.0526 g/L/h, 6.31 g/L 4-HBC from 20 g/L phytosterol) in an industrial resting cell bio-transformation system.Conclusion sIncreasing cell permeability combined with the co-overexpression of the key genes (cyp125, choM1, and fadA5) involved in the conversion pathway of sterol to 4-HBC was effective to enhance the productivity of 4-HBC. The strategy might also be useful for the conversion of sterol to other steroidal intermediates by mycobacteria.
研究领域:
生物工程
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微生物学
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制药工程
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
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IF 6.182
Guomin Geng,Mengyang Cai,Rui Fang,Qian Luan,Zhiqiang Zhang,Jianjun Song,Jian Zhang
Developing highly efficient and stable catalysts toward advanced oxidation technology is the key to achieve industrial peroxymonosulfate (PMS) activation for pollution degradation. Perovskite-type oxide catalysts show high activity and stability for PMS activation, yet the small specific surface area of perovskite catalysts limits the increase of catalytic performance. Metal-organic frameworks (MOFs) have been widely used as the templates/precursors to prepare high specific surface area nanomaterials. In this study, two kinds of high surface area perovskites LaCoO 3 and LaMnO 3 were firstly fabricated with MOFs as templates. Benefiting from the extraordinary skeleton structure of MOFs, well-defined architecture and porous perovskite catalysts show superior catalytic performance towards PMS activation for the degradation of 2, 4-dichlorophenol (2, 4-DCP), with a 99.8% degradation of 2, 4-DCP in 25 min for LaCoO 3 catalyst. Moreover, the LaCoO 3 catalysts exhibit long-term stability with a slight loss of catalytic activity during 3 times recycled experiments. This study not only developed high activity catalysts for PMS activation, but also expanded the MOFs applications for environment and energy.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Jiaojiao Yang,Binyu Guo,Lei Li,Quanyuan Chen,Chensi Shen,Juan Zhou
The limited regeneration of Fe 2+ in the Fe-catalyzed advanced oxidation processes (AOPs) constrained its application for the removal of organic pollutants. Herein, MoSe 2 was introduced to promote the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) in the Fe 2+ /PMS system. Compared with Fe 2+ /PMS processes, the 2,4-D degradation efficiency and PMS decomposition rate respectively increased by 73.8% and 84.2% in the MoSe 2 /Fe 2+ /PMS system. DFT simulation results suggested that Se atoms acted smoothly as the bridge supporting the charge transfer from Mo to adjacent Fe atoms, which led to the reduction of Fe 3+ . The rapid regeneration of Fe 2+ boosted the activation of PMS and the degradation of pollutants. Additionally, the electron paramagnetic resonance (EPR) and quenching experiments results indicated that SO 4 ∙ − , ∙OH, and 1 O 2 accounted for 2,4-D degradation, and SO 4 ∙ − and 1 O 2 predominated the reaction. The Mo based co-catalysts showed better co-catalytic effect than the W counterparts, and the moderate adsorption for PMS and lower electron transfer electron transfer resistance accounted for the more excellent co-catalytic performance of MoSe 2 than that of WSe 2 . In addition, the degradation efficiency of 2,4-D was up to 95.5% after five cycles of MoSe 2 in the co-catalytic system. The coexistent humic acid (HA) and Cl − showed ignorant negative effect on the degradation, while HCO 3 − would depress the oxidation reaction. The acidic etching wastewater can be applied as the Fe ions source in this co-catalytic process to remove 2,4-D effectively.
研究领域:
环境科学
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化学工程
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2013
·Vol. 24
·期 24
·245601
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IF 3.842
Miao Liang,Libing Wang,Xia Liu,Wei Qi,Rongxin Su,Renliang Huang,Yanjun Yu,Zhimin He
Bio-nanomaterials fabricated using a bioinspired templating technique represent a novel class of composite materials with diverse applications in biomedical, electronic devices, drug delivery, and catalysis. In this study, Au nanoparticles (NPs) are synthesized within the solvent channels of cross-linked lysozyme crystals (CLLCs) in situ without the introduction of extra chemical reagents or physical treatments. The as-prepared AuNPs-in-protein crystal hybrid materials are characterized by light microscopy, transmission electron microscopy, x-ray diffraction, and Fourier-transform infrared spectroscopy analyses. Small AuNPs with narrow size distribution reveal the restriction effects of the porous structure in the lysozyme crystals. These composite materials are proven to be active heterogeneous catalysts for the reduction of 4-nitrophenol to 4-aminophenol. These catalysts can be easily recovered and reused at least 20 times because of the physical stability and macro-dimension of CLLCs. This work is the first to use CLLCs as a solid biotemplate for the preparation of recyclable high-performance catalysts.
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DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2017
·
IF 4.536
Jinsong Chai,Ying Lv,Sha Yang,Yongbo Song,Xiaofeng Zan,Qinzhen Li,Haizhu Yu,Mingzai Wu,Manzhou Zhu
In this work, we report the synthesis and crystal structure of Au38–xCux(2,4-DMBT)24 (x = 0–6, 2,4-DMBTH = 2,4-dimethylbenzenethiol) alloy nanocluster for the first time. A variety of characterizations including ESI-MS, TGA, and XPS reveal the composition as Au38–xCux(2,4-DMBT)24 (x = 0–6). The single crystal structure has been determined by an X-ray single crystal diffractometer. From the anatomy of the structure, a bi-icosahedral Au23 core is protected by six dimeric [−SR–M–SR–M–SR−] units (M = Cu/Au) and three monomeric [−SR–Au–SR−] units. It is interesting that all the Cu atoms are selectively doped in the motifs of the Au38–xCux(2,4-DMBT)24 nanocluster. This phenomenon is distinct from the exclusive core doping of the Ag atoms in the previously reported Au38–xAgx alloy. Both the experimental results and DFT calculations of UV–vis spectra imply that the optical property of the Au38–xCux(2,4-DMBT)24 nanocluster is consistent with that of the Au38(2,4-DMBT)24 nanocluster, because the Cu dopants make little contribution to the frontier orbitals of the alloy NC.
研究领域:
材料科学
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化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jiaoqin Liu,Jingjing Zhang,Mengye Jia,Rui Ma,Ruijuan Qu,Zunyao Wang,Zhaoguo Liu,Yu Jiang,Haoxiang Zhang,Yujia Bai,Riya Jin,Yina Qiao,Jiangtong Wu
An efficient nitrogen (N)-ZnO photocatalytic composite was synthesized and well characterized to demonstrate its superior photocatalytic performance as well as the degradation kinetics of 24 ILs in an N-ZnO/Xe lamp irradiation system, as well as the pathways and mechanisms of the photocatalytic degradation (PCD) of typical ionic liquids [OMIm]Br was systematically studied. To investigate the catalytic effect, nitrogen was synthesized from various inorganic and organic nitrogen compounds to ZnO. The NH 4 NO 3 -ZnO composite calcined at 300 °C with a molar ratio of NH 4 NO 3 to Zn(NO 3 ) 2 ·6H 2 O of 10:1 exhibited superior catalytic performance as assessed by SEM, TEM, FT-IR, UVDRS, BET surface area, XPS, and XRD characterizations. After 24 h of exposure to Xe lamp irradiation, all 24 ILs were significantly degraded by the introduction of 0.125 g/L of catalyst, whereas simulated sunlight alone resulted in minimal degradation. The pseudo-first-order rate coefficients ( k ) for PCD were closely related to the carbon chain length of the alkyl substituents and the type of anion. Multiple linear regression analysis indicated no linear correlation between the parameters E , H°, E th , and C v° from the Gaussian calculation and k . Conversely, a positive correlation was observed for E HOMO (B=0.742, β = 1.282, P = 0.011), q H+ (B=1.040, β = 3.284, P = 0.006), and G° (B=0.001, β = 5.400, P = 0.007) with k . Mass spectrometry analysis revealed that the primary PCD pathway for [OMIm]Br involved C-N and/or C-C bond breaking, mono-, di-, and tri-hydroxylation, single and double oxygen transfer, and bromo-substitution reactions.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2026
·
IF 14
Hao Liu,Jinmei Cai,Ting Li,Yuan Zhang,Mingxuan Lv,Boyang Hu,Huayi Fang,Baoan Li
A maize-stem-inspired phase change composite was designed for all-day power generation applications. • Adaptively integrate solar photothermal conversion, latent heat storage, and radiative cooling within a single material. • Aligned GO/SiO 2 porous framework provides efficient heat pathways, stable paraffin encapsulation, and high mid-infrared emissivity. • Outdoor tests and simulations across 39 global cities demonstrate adaptive 24 h electricity generation from solar energy and cold space. Solar-thermal-electric conversion represents a promising pathway for off-grid power generation, yet its practical application is constrained by the low output density and intermittent nature of solar radiation. Herein, we design a maize-stem-inspired multifunctional phase change composite (PCC) that synergistically integrates latent heat storage and radiative cooling within a single material, enabling adaptive, uninterrupted electricity generation over 24 h. The composite is fabricated by encapsulating paraffin wax (PW) within a three-dimensional porous graphene oxide/silica (GO/SiO 2 ) framework, and exhibiting a latent heat of 130.2 J g −1 , a photothermal conversion efficiency of 90.7% and an energy storage capacity of 98.46%. When coupled with a thermoelectric module, the resulting system realizes continual 24 h electric energy generation by leveraging its combined photothermal conversion, energy storage, and radiative cooling functionalities. Under an irradiance of 500 mW cm −2 , the system delivers an output power density of 41.51 W m −2 . The innovative material design merges photothermal conversion, phase change energy storage, and passive radiative cooling within a single substance to adaptively capture energy from solar radiation and cold space. The study provides new insights into the efficient storage and utilization of solar energy, offering significant scientific value for developing uninterrupted solar-thermal-electric energy generation systems. Download: Download high-res image (349KB) Download: Download full-size image
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DOI
高影响因子
Journal of the American Chemical Society
·
2025
·
IF 15.6
Chenyang Nie,Yuanhao Li,Xiaona Liu,Nana Yan,Chao Ma,Jiahui Zhu,Linlin Hao,Peng Guo,Zhongmin Liu
Small-pore aluminophosphate (AlPO) molecular sieves (MSs), particularly those in the ABC-6 family, have gained significant attention for their unique capabilities in selective adsorption and energy storage. However, developing new synthesis strategies for designing AlPOs with three-dimensional (3D) channel systems and determining the structures of nanosized novel AlPO MSs remain challenging tasks. In this study, we introduce a new small-pore AlPO with a 3D channel system, named DNL-17, synthesized by using a diquaternary ammonium compound as an organic structure-directing agent (OSDA). Its crystallographic structure was determined through advanced 3D electron diffraction (ED) techniques and further confirmed by emerging integrated differential phase contrast imaging. DNL-17 exhibits a remarkable 24-layer stacking sequence along the c-axis and features a variety of composite building units (CBUs), including d6r, can, cha, and eri. Significantly, a combination of 3D ED, theoretical calculations, and solid-state 13C NMR reveals that the employed OSDA adopts distinct conformations to stabilize different cages. This finding highlights a novel strategy for constructing AlPO MSs with diverse cage-based CBUs. Furthermore, DNL-17 demonstrates unique selective adsorption properties, particularly in the separation of n-butane and isobutane.
研究领域:
材料科学
·
化学
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DOI
PubMed
高影响因子
PeerJ
·
2025
·
IF 2.4
Jia Song,Jinyu Qiao,Mingxue Chen,Jiahui Li,Jixia Wang,Dayong Yu,Huachuan Zheng,Liying Shi
Chaetoglobosin A (ChA) is an antitumor compound produced by Chaetomium globosum . However, the mechanism of its antitumor effect has been rarely reported. In this study, we evaluated the anti-proliferative effect of ChA on T-24 human bladder cancer cells and explored its mechanism of action. ChA was found to have a good inhibitory effect on T-24 cells by MTT assay with an IC50 value of 48.14 ± 10.25 μΜ. Moreover, it was found to have a migration inhibitory ability and a sustained proliferation inhibitory effect on tumor cells by cell aggregation assay and cell migration assay. The cells morphological changes were determined by Hoechst33342 assay. While Annexin V-FITC/PI double-staining assay also demonstrated that the number of apoptotic cells increased with the increase of drug concentration. Flow cytometry results showed that ChA treatment increased reactive oxygen species (ROS) and decreased mitochondrial membrane potential (MMP) in T-24 cells and inhibited cell mitosis, resulting in an increase in the number of sub-G1 phase cells. Further western blot experiments demonstrated that MAPK and PI3K-AKT-mTOR pathways were activated after drug treatment in addition to endogenous and exogenous apoptotic pathways. The addition of the ROS inhibitor N-acetylcysteine (NAC) upregulated the expression level of Bcl-2 protein, decreased p38 phosphorylation, increased ERK phosphorylation and restored the levels of PI3K and p-mTOR after ChA treatment. These suggest that ChA induces apoptosis by regulating oxidative stress, MAPK, and PI3K-AKT-mTOR signaling pathways in T-24 cells.
研究领域:
肿瘤学
·
细胞生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Science of Advanced Materials
·
2018
·Vol. 10
·期 9
·1241-1249
·
IF 1.318
Chen Huajun,Li Dong,Ding Wuxiu
Mesoporous manganese dioxide(meso-MnO2) fibers were synthesized in high yields in a simple, templatefree manner by localized Ostwald ripening under hydrothermal conditions. The adsorption behavior of 2,4-dinitrophenol on meso-MnO2 fibers was well described by Langmuir
isotherm equation, Dubinin-Radushkevich and pseudo-second-order kinetic equation, so the adsorption mechanism maybe a chemical and monolayer adsorption. Meso-MnO2 fibers have potential for use as a high-performance adsorbent.
研究领域:
材料科学
·
环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）