ChemCatChem
·
2024
·
IF 3.8
Li Zhang,Haiyan Xu,Xiaofan Wu,Yitong Li,Yuan Tao,Zedu Huang,Fener Chen
Graphical Diversification of commercially available 3’-amino-3’-deoxythymidine via nucleoside phosphorylase Ec PPNP- and/or nucleoside 2’-deoxyribosyltransferase Ll NDT-catalyzed transglycosylation has been demonstrated as a generally applicable, straightforward means to access a variety of valuable 3’-amino containing nucleosides in good yields. The synthetic utility was further showcased by the successful synthesis of 3’-amino-2’,3’-dideoxy-5-bromouridine in 29% isolated yield at a preparative scale. A systematic study on the enzymatic transglycosylation of 3′-amino nucleosides has been reported. Using the commercially available 3′-amino-3′-deoxythymidine as the sugar donor, 13 pyrimidine and purine nucleobases were shown as the viable sugar acceptors in the nucleoside phosphorylase Ec PPNP- and/or nucleoside 2′-deoxyribosyltransferase Ll NDT-catalyzed reactions, furnishing the corresponding 3′-amino containing nucleosides in 23%–73% NMR yields at an analytical scale. The synthetic utility of this diversification approach was further showcased by the successful synthesis of 3′-amino-2′,3′-dideoxy-5-bromouridine in 29% isolated yield at a preparative scale. The current work demonstrates the transglycosylation activity of Ec PPNP for the first time, expands the substrate scope of Ll NDT, and provides an efficient, direct access to various 3′-amino nucleosides of high value.
研究领域:
生物化学
·
药物化学
·
酶学
✓ 已验证
DOI
高影响因子
Bioresources and Bioprocessing
·
2018
·Vol. 5
·期 1
·1-11
·
IF 0
Liu Xin,Wang Weiwei,Hu Haiyang,Lu Xinyu,Zhang Lige,Xu Ping,Tang Hongzhi
Background Benzofuran and its derivatives contain central pharmacophores and are important structures in medicinal chemistry. Chemical synthesis of benzofuran rings often requires expensive catalysts and stringent operational conditions. Biosynthesis is recognized as a promising way to save energy and produce valuable compounds. Dioxin biodegradation pathways can form several benzofuran derivatives, and these pathways may be a better choice for further synthesis of important biological compounds. 2-Hydroxy-4-(3′-oxo-3′ H -benzofuran-2′-yliden)but-2-enoic acid (HOBB), a benzofuran derivative, can be biosynthesized from dibenzofuran (DBF) through co-metabolic degradation in a lateral dioxygenation pathway. Results Efficient biosynthesis of HOBB was observed using whole cells of Pseudomonas putida strain B6-2. After cultivation in LB medium containing biphenyl, the cells were suspended to an OD 600 of 5 to conduct biosynthesis in the presence of 0.5-mM DBF at pH 7 for 8 h. The bacterial cells were used twice to degrade approximately 0.70-mM DBF, and in batch process, accumulated about 0.29-mM HOBB. HOBB could be easily purified from the reaction with ethyl acetate using the neutral-acid extraction method, and 13.58 ± 0.31 mg of HOBB was obtained from 22.49 ± 0.74-mg DBF with an overall production yield of 60.4% (w/w). The product HOBB, which is a yellow powder, could be detected and identified by LC–MS, GC–MS, and NMR. Conclusions In this study, a new biological route was developed to produce HOBB from DBF using whole cells of P. putida B6-2 (DSM 28064). The biosynthesis of HOBB may contribute to studies of the DBF lateral pathway and provide a new green route for synthesizing benzofuran derivatives with pharmacological activities.
研究领域:
环境生物学
·
药学
·
微生物学
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产品关联
DOI
FOOD CHEMISTRY
·
2022
·
IF 9.231
Cong Chen,Fei Yu,Xinli Wen,Shuna Chen,Kaixi Wang,Feiquan Wang,Jianming Zhang,Yuanyuan Wu,Puming He,Youying Tu,Bo Li
Two major green leaf volatiles (GLVs) in tea that contribute greatly to tea aroma, particularly the green odor, are (E)-2-hexenal and (Z)-3-hexenal. Until now, their formation and related mechanisms during tea manufacture have remained unclear. Our data showed that the contents of (E)-2-hexenal and (Z)-3-hexenal increased more than 1000-fold after live tea leaves were torn. Subsequently, a new (Z)-3:(E)-2-hexenal isomerase (CsHI) was identified in Camellia sinensis . CsHI irreversibly catalyzed the conversion of (Z)-3-hexenal to (E)-2-hexenal . Abiotic stresses including low temperature, dehydration, and mechanical wounding, did not influence the (E)-2-hexenal content in intact tea leaves during withering, but regulated the proportions of (Z)-3-hexenal and (E)-2-hexenal in torn leaves by modulating CsHI at the transcript level. For the first time, this work reveals the formation of (E)-2-hexenal during tea processing and suggests that CsHI may play a pivotal role in tea flavor development as well as in plant defense against abiotic stresses.
研究领域:
植物学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2014
·
IF 1.251
Fengting Chen,Jiachang Zhao,Jingli Xu,Yuandong Wu
The hetero-bimetallic polyanionic compound BiCu 2 (TeO 3 )(SO 4 )(OH) 3 was synthesized using hydrothermal techniques. Single-crystal X-ray diffraction analysis revealed that title compound crystallizes in the monoclinic space group P 2 1 / n (No. 14) with lattice parameters a = 9.5513(15), b = 6.3022(10), c = 13.955(2) Å, β = 102.845(3)°, and Z = 4. The structure of title compound is a three-dimensional tunnel framework consisting of edge-sharing two-dimensional [Cu 3 O 10 ] 14– layers linked by BiO 8 bicapped trigonal prisms, TeO 3 2– trigonal pyramids and SO 4 2– tetrahedra. Along [010], each Cu(1)O 6 octahedron shares two edges with adjacent Cu(1)O 6 units to form infinite one-dimensional zigzag chains, which are connected by double inverted Cu(2)O 6 octahedral units via edge sharing (O9 ··· O8). The suitability of crystal for optical applications was investigated by UV/Vis/NIR spectroscopy. Raman and FTIR spectra were used to confirm the presence of various functional groups such as TeO 3 2– , SO 4 2– , OH – in the crystals.
研究领域:
无机化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Lei Zhang,Xuefeng Zhang,Chaoyong Zhao,Xuan Xiao
Eu 3+ -activated Sr 3 −x Ca x La(VO 4 ) 3 phosphors were fabricated via citric-acid-assisted sol combustion. Characterization of the Sr 3 −x Ca x La(VO 4 ) 3 :Eu 3+ samples with different concentrations of Ca 2+ revealed a hexagonal crystal structure belonging to the R -3 m space group. The amount of Ca 2+ added ( x ) was controlled within 0 ≤ x ≤ 2 to yield high-purity phosphors. Scanning electron microscopy results showed that an increase in Ca 2+ concentration resulted in a decrease in the particle size of Sr 3 −x Ca x La(VO 4 ) 3 :Eu 3+ , with the shape gradually changing from nearly equiaxed to lath-shaped. The Sr 2 CaLa(VO 4 ) 3 :Eu 3+ phosphor (denoted as SCLVO:Eu 3+ ) exhibited the strongest photoluminescence (PL) intensity at 618 nm among the samples under excitation of 394-nm near-UV (NUV) light. The study of Eu 3+ doping concentration confirmed that Eu 3+ could enter the lattice of the SCLVO matrix without altering its crystal structure. SCLVO:Eu 3+ was found to strongly absorb 394 nm NUV light and 464 nm blue light. The optimal concentration of the Eu 3+ dopant in the SCLVO host was 0.11, which resulted in the phosphor achieving an excellent PL intensity and a color purity of 98.68%. Tunable luminescence from the orange area (0.5280, 0.4522) of Commission Internationale de l'éclairage (CIE) to the red area (0.6313, 0.3650) was achieved by adjusting the concentration of Eu 3+ . Under 394 nm excitation, SCLVO:0.11Eu 3+ phosphor has a quantum yield (QY) of 28.2% and excellent thermal stability with 0.383 eV activation energy . Consequently, White-light-emitting diode (WLED) based on SCLVO:0.11Eu 3+ phosphor yielded a high color rendering index (CRI), low correlated color temperature (CCT), and CIE coordinates of 91.8, 5196 K, and (0.3407, 0.3612), respectively, under the 20 mA driven current. These results indicated the tremendous potential of SCLVO:0.11Eu 3+ phosphors for application in WLEDs excited by NUV or blue light.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2023
·Vol. 225
·期 5
·2300361
·
IF 2.5
Dan Liu,Hui Liang,Jiang Lu
A novel monomer, N -(2-((3-morpholine-3-isopropyl)thio)ethyl)methacrylamide (MTMA), is synthesized and polymerized by reversible addition-fragmentation chain transfer (RAFT) polymerization to give a thermoresponsive polymer poly[ N -(2-((3-morpholino-3-oxopropyl)thio)ethyl)methacrylamide] (PMTMA). PMTMA exhibits reversible thermoresponsive phase transitions of the lower critical solution temperature (LCST) type in water and the upper critical solution temperature (UCST) type in ethanol and methanol. The degree of polymerization and concentration of PMTMA, and solvent type have a significant influence on the transition temperature. PMTMA shows complicated thermoresponse in water–alcohol mixtures. In water–ethanol mixtures, the phase diagram of PMTMA can be divided into three regions according to ethanol volume percentages, φ : 0 ≤ φ < 15% for an LCST region; 15% ≤ φ < 91.5% for a complete dissolution region; 91.5% ≤ φ ≤ 100% for a UCST region. At high water content, the LCST increases with increasing ethanol content; and at high ethanol content, the UCST decreases with increasing water content. In water–methanol mixtures, PMTMA shows more complex thermoresponse. In the range of 0–50 °C, the phase diagram can be divided into five regions, depending on the methanol volume percentages φ : 0 ≤ φ < 15% for LCST region; 15% ≤ φ < 25% for immiscible region; 25% ≤ φ < 85% for UCST region; 85% ≤ φ < 95% for fully dissolved region; 95% ≤ φ ≤ 100% for another UCST region.
研究领域:
高分子化学
·
材料科学
·
物理化学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-12
·
IF 5.3
Xiao Rongsheng,Dai Hongliang,Liu Xingyu,Qi Jingwen,Bao Meishuo,Wang Xingang,Li Mingde,Zhang Wuxiang,Yan Dengxin
A novel colorimetric approach specifically designed to effectively identify the presence of 3-aminophenol (3-AP) in environmental water is introduced. Briefly, a nitrogen-doped carbon-supported cobalt nanozyme (Co@CN-1) was synthesized and utilized to improve the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of peroxymonosulfate (PMS). Comparative catalytic reactions confirmed that the performance of PMS as an activator exceeds that of hydrogen peroxide catalytically by a factor of 3.5. The catalytic reaction parameters underwent optimization, further resulting in the derivation of a linear detection equation for 3-AP, expressed as inhibition rate (IR%) = 3.35[3-AP]—4.36 (0–20 μM, R 2 = 0.994) and IR% = 1.43[3-AP] + 31.87 (20–36 μM, R 2 = 0.992), with the limit of detection (LOD) of 2.84 μM. The linear relationship between 3-AP concentration and the conversion of color to grayscale value (GSV) was established by smartphones, expressed as GSV = 1.28[3-AP] + 147.10 ( R 2 = 0.972). Density functional theory calculations revealed that Co acts as the preferred active site for donating electrons in PMS activation. This work provides a rapid and accurate approach for monitoring 3-AP concentration, enabling real-time analysis and potentially contributing to environmental and ecological studies. Graphical Abstract
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2016
·Vol. 100
·期 1
·265-275
·
IF 2.787
Longwen Wu,Xiaohui Wang,Zhengbo Shen,Longtu Li
The (1− x )BaTiO 3 – x Bi(Zn 2/3 Nb 1/3 )O 3 ( x = 0.01–0.30) ceramics were synthesized by solid-state reactions. The solubility limit was determined to be x = 0.20. A systematic structural transition from a tetragonal phase ( x ≤ 0.034), to a mixture of tetragonal and rhombohedral phases (0.038 ≤ x ≤ 0.20), and finally to a pseudocubic phase ( x ≥ 0.22) at room temperature was identified. Dielectric measurement revealed a ferroelectric ( x ≤ 0.04) to relaxor ( x ≥ 0.06) transition with permittivity peak broadening and flattening, which was further verified by Raman spectroscopy and differential scanning calorimetry (DSC). Activation energies obtained from the Vogel–Fulcher model displayed an increasing trend from ~0.03 eV for x ~ 0.05, to unusually high values (>0.20 eV) for the compositions with x ≥ 0.15. With the increase in Bi(Zn 2/3 Nb 1/3 )O 3 content, the polarization hysteresis demonstrated a tendency from high nonlinearity to sublinearity coupled with the reduction in remnant polarization and coervice field. The deconvolution of the irreversible/reversible polarization contribution was enabled by first-order reversal curve distributions, which indicates that the decreasing polarization nonlinearity with the increase in Bi(Zn 2/3 Nb 1/3 )O 3 concentration could be related with the change from the ferroelectric domain and domain wall contributions to the weakly coupled relaxor behaviors.
研究领域:
材料科学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 16
·期 2
·276-283
·
IF 3.1
Ting Xu,Qing Fu,Zeng Qingru,Zhaojie Wang,Xin Liu,Shensheng Xiao,Xiaoming Jiang,Yuepeng Lu,Zhiyong Gong,Yongning Wu,Min Fang
A rapid fluorescence detection method was established for 3-monochloropropane-1,2-diol (3-MCPD). The detection system works based on the fluorescence quenching of pyrocatechol–polyethyleneimine (PCh–PEI) polymer by 3-MCPD. The fluorescence quenching ability of 3-MCPD for PCh–PEI polymer was measured at different pH and temperatures. Indeed, in the presence of 3-MCPD, the fluorescence intensity of PCh–PEI polymer solution was quenched best at 100 °C and pH 8.5. Also, the effect of different concentrations of 3-MCPD on the optical properties of the PCh–PEI polymer was examined. Under optimal experimental conditions, fluorescence detection was linear in a range of 0.08–2.0 mg per L 3-MCPD, with a calculated detection limit of 0.06 mg L−1 and a correlation coefficient of 0.9974. Concisely, the reported method has good sensitivity and can be used for the rapid detection of 3-MCPD contamination in food products.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INSTRUMENTATION SCIENCE & TECHNOLOGY
·
2017
·
IF 0.874
Shuyan Niu,Yajie Fang,Kun Zhang,Juan Sun,Jun Wan
A facile and sensitive fluorescent strategy for the determination of dopamine is reported using 2, 3-diaminophenazine. Dopamine caused autooxidation in the presence of oxygen with catalysis by Mn2+ and formed 2, 3-dihydro-1H-indole-5,6-dione. 2, 3-Diaminophenazine provided relatively strong fluorescence. 2, 3-Diaminophenazine in aqueous solution caused fluorescence quenching when the aminochrome condensed with 2, 3-diaminophenazine to allow the determination of dopamine. Under the optimized conditions, the linear dynamic range for dopamine was from 2.0 × 10−6 mol · L−1 to 6.1 × 10−5 mol · L−1 with a detection limit of 1.76 × 10−6 mol · L−1.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CLAY SCIENCE
·
2019
·
IF 3.89
Hui-Ran Jin,Xin-Yu Zhou,Yong Zhu,Guo-Fan Sun,Shi-Jie Ding,Jing Chen
In this paper, non-aqueous Fe(OH) 3 /palygorskite dispersion was prepared and its liquid crystal phase behavior was investigated. Positively charged Fe(OH) 3 colloidal particles were put in to non-aqueous and organically modified palygorskite dispersion. The effects of organically treated palygorskite and Fe(OH) 3 on the preparation of stable palygorskite dispersion were characterized with FT-IR, XRD , Contact Angle measurement , Zeta potential detection, Settlement measurement and SEM , respectively. The addition of Fe(OH) 3 particles shifted the concentrations of the coexisting isotropic and nematic phases to higher values while at the same time markedly accelerated the phase separation. The influence of Fe(OH) 3 particles on the dispersion in magnetic field was also discussed.
研究领域:
材料科学
·
胶体化学
✓ 已验证
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2024
·
IF 3.2
Yanbo Li,Jinsuo Yu,Deyu Wei,Chengkai Liu,Yan Zhao,Junjia Bian,Shulin Sun,Hongwei Pan,Huili Yang,Lijing Han,Huiliang Zhang
Biodegradable materials provide protective properties comparable to conventional plastics while diminishing reliance on nonrenewable resources. Environmentally friendly polymer blends were prepared by melting poly(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) (PHBHHx) with poly (butylene succinate) (PBS). PBS was synthesized through esterification and polycondensation using 1,4-butanediol and succinic acid, with tetrabutyltitanate as the catalyst. The PHBHHx is supplied by Bulepha®PHA Company of China, model number BP330. The effects of PHBHHx content on thermal behavior, compatibility, and thermal stability of PBS/PHBHHx blends were systematically investigated. Differential scanning calorimetry (DSC) results indicated that the addition of PHBHHx influenced the crystallization behavior, the crystallization temperature ( T c ) of PBS decreased meanwhile, the crystallization half-time of PBS is reduced to 1.5 min compared with neat PBS (5 min) after isothermal is crystallized at 95°C. Polarized Optical Microscopy (POM) images also revealed that crystallization rate accelerated in the blend with increasing PHBHHx content. Rheological and Scanning Electron Microscopy (SEM) analysis demonstrated that incorporating a significant content of PHBHHx reduced compatibility between PBS and PHBHHx. Mechanical property testing indicates that the addition of PHBHHx reduced the toughness of blends. Additionally, the addition of a small amount of PHBHHx imparts a higher crystallinity (56%) and heat resistance of the PBS/PHBHHx blends (78°C) was higher than neat PBS (74°C). Highlights Laboratory synthesis of high molecular weight PBS. Optimal ratios improved crystallinity and thermal stability of blends. A systematic study of the crystallization properties of blends.
研究领域:
材料科学
·
高分子科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）