BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Cuiying Lin,Qingqing Huang,Xiang Hong,Shichai Hong,Xiaolong Shu,Enci Wang,Lixin Wang,Weiguo Fu,Zhenyu Lin
Vascular endothelial growth factor (VEGF 165 ) is a signal protein that plays a central role in the regulation of angiogenesis and can stimulate angiogenesis. The development of highly sensitive and selective detection method for VEGF 165 is very important for disease diagnosis and follow-up treatment monitoring. In this study, an electrochemiluminescence (ECL) aptasensor for VEGF 165 has been developed based on quench of H 2 O 2 toward Ru(bpy) 3 2+ /TPrA ECL system and RecJ f exonuclease induced target recovery and hybridization chain reaction (HCR) as amplification strategy. The presence of VEGF 165 makes a large number of glucose oxidase (GOD) fixed on the electrode surface through the double signal amplification strategies. The present of GOD cause the production of a large amount of H 2 O 2 near the electrode surface under excess amount of glucose, resulting in the inhibition of the ECL signal of Ru(bpy) 3 2+ /Au nanoparticles (Ru(bpy) 3 2+ /AuNPs) film fixed on the electrode surface. The ECL response of the designed biosensor has a good linear relationship with the logarithm of the concentration of VEGF 165 in the range of 0.5 pg/mL to 500 ng/mL with a detection limit of 0.2 fg/mL. The VEGF 165 in serum samples has been detected by the proposed aptasensor with satisfactory results.
研究领域:
生物传感器
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分析化学
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生物医学工程
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分子生物学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Advanced Electronic Materials
·
2024
·
IF 5.3
Wei Wang,Yujun Deng,Shuai Sun,Massimiliano Galluzzi,Yang Jiao,Paul K. Chu,Zeren Li,Jia Li,Jianquan Yao
Visible-blind ultraviolet (VBUV) photodetectors are designed for UV detection without responding to visible light, thus having many applications in communications, flame detection, environment monitoring, and astronomy. Herein, an organic device concept based on the bulk heterojunction (BHJ) photodiode is presented for high-performance VBUV photodetection and imaging. The BHJ, comprised of an organic electron donor, 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene (C8-BTBT), and an electron acceptor, indene-C 60 bisadduct (ICBA), shows efficient generation and transport of free charges upon UV irradiation. The organic photodiode (OPD) delivers exceptional VBUV photodetection performance. At a low voltage of −0.5 V, the device exhibits a wide linear dynamic range of 98.15 dB. Furthermore, the OPD can detect ultra-low light intensities down to 0.58 µW cm −2 with a high photoresponsivity of 0.12 A W −1 and specific detectivity of 2.75 × 10 12 Jones. More importantly, by integrating the OPD with a readout integrated circuit, the pixel-array organic VBUV imager is demonstrated to have high-quality imaging capability. The results reveal a novel strategy to design VBUV photodetectors and imagers with balanced performance, cost, area, flexibility, and power consumption.
研究领域:
光子学
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材料科学
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光电技术
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半导体器件
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产品关联
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Yue Wang,Qihang Ding,Gongcheng Ma,Zhiwei Zhang,Jiaqi Wang,Chang Lu,Chunbai Xiang,Kun Qian,Jun Zheng,Yaming Shan,Pengfei Zhang,Zhen Cheng,Ping Gong,Qi Zhao
Bacterial pneumonia has garnered significant attention in the realm of infectious diseases owing to a surge in the incidence of severe infections coupled with the growing scarcity of efficacious therapeutic modalities. Antibiotic treatment is still an irreplaceable method for bacterial pneumonia because of its strong bactericidal activity and good clinical efficacy. However, the mucus layer forming after a bacterial infection in the lungs has been considered as the “Achilles’ heels” facing the clinical application of such treatment. Herein, traceable biomimetic nanoantibiotics (BioNanoCFPs) were developed by loading indacenodithieno[3,2-b]thiophene (ITIC) and cefoperazone (CFP) in nanoplatforms coated with natural killer (NK) cell membranes. The BioNanoCFP exhibited excellent demonstrated mucus-penetrating abilities, facilitating their arrival at the infection site. The presence of Toll-like receptors in the NK cell membrane rendered the BioNanoCFP with the capability to recognize pathogen-associated molecular patterns within bacteria, allowing precise targeting of bacterial colonization sites and achieving substantial therapeutic efficacy. Overall, our findings demonstrate the viability and desirability of using NK cell membrane-mediated drug delivery as a promising strategy for precision treatment.
研究领域:
药学
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纳米技术
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传染病学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Yu-Ling Wang,Fu-Rao Liu,Jun-Tao Cao,Shu-Wei Ren,Yan-Ming Liu
The identification of tumor markers is of great importance for clinical diagnosis but accurate detection with high sensitivity is still a great challenge. In present work, a spatial-resolved dual-signal-output electrochemiluminescent (ECL) ratiometric assay platform was constructed for sensitive detection of prostate specific antigen (PSA) on a dual-disk glassy carbon electrode. To fabricate the platform, flower-like CdS three-dimensional (3D) assemblies and Ru(bpy) 3 2+ -conjugated silica nanoparticles (Ru(bpy) 3 2+ @RuSi NPs), were immobilized onto the two disks as cathodic and anodic ECL emitters, respectively. After the stepwise modification of the gold nanoparticles , antibody for PSA, and bovine serum albumin onto the two disks respectively, the Ru(bpy) 3 2+ @RuSi NPs-based disk were incubated with varied concentration of PSA as working electrode , whereas the flower-like CdS 3D assemblies-based disk with fixed concentration of PSA were taken as internal reference electrode . The label free assay of PSA was realized by the ratio of anodic ECL signal from working electrode to the cathodic ECL signal from the internal reference electrode (ECL anode /ECL cathode ). On the basis of the spatial-resolved dual-signal-output ratiometric ECL sensor, the PSA can be detected accurately with a linear range of 0.001 – 50 ng/mL at a concentration as low as 0.34 pg/mL. Furthermore, the proposed method was applied for PSA determination in human serum samples with satisfying results. Thanks to the same modified process of the two disks, this universal design well avoids environmental errors including the interference caused in the biological recognition process, which effectively reduces the false positive or negative errors, exhibiting a greatly improved accuracy, reliability and sensitivity.
研究领域:
分析化学
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生物化学
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生物医学工程
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临床诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaoqing Wang,Huiyi Chen,Jihui Zhang,Hong Zhou,Xiangying Meng,Na Wang,Yishan Fang,Bo Cui
The detection of Escherichia coli ( E. coli ) is of great significance for the environment and human health. Herein, a photoelectrochemical (PEC) detection strategy based on molecularly imprinted polymers (MIPs) was proposed for the sensitive detection of E. coli . 4,4′,4″-Trinitrotriphenylamine (TPA-NO 2 ) was prepared using a simple nitration reaction. Subsequently, MIP films were polymerized on the surface of TPA-NO 2 using 1,3-dihydrothieno[3,2- d ]pyrimidine-2,4-dione as the functional monomer with the dual functions of specific recognition and sensitization. The linear range was 10–10 8 CFU/mL and the limit of detection was 10 CFU/mL. It showed favorable recoveries in real sample tests of milk, orange juice and tomato. Additionally, the ability of functional monomers to bind excellently with E. coli was verified using molecular docking techniques. This research provided broader possibilities for constructing MIPs-PEC sensors and analyzing the interaction mechanism between E. coli and functional monomers.
研究领域:
分析化学
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生物技术
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Qingqing Huang,Fang Luo,Cuiying Lin,Jian Wang,Bin Qiu,Zhenyu Lin
Ru(dcbpy) 3 2+ -polyethyleneimine-L-lysine (Ru-PEI-L-lys) had been immobilized on metal organic frameworks (ZIF-8) to form an electrochemiluminescent(ECL) indicator (Ru-PEI-L-lys-ZIF-8). In this ECL indicator, PEI-L-lys is used as a co-reactant. Platinum nanoparticles (PtNPs) has been mixed with Ru-PEI-L-lys-ZIF-8 to form a thin film to increase the electron transfer rate and enhanced the ECL response of the system. The prepared material had been characterized carefully and been combined with high selectivity of aptamer to develop a ECL biosensor for thrombin detection. RecJ f exonuclease (an ssDNA specific exonuclease) assistant target recycling amplification has been adopted to enhance the sensitivity of the system. The ECL response of the system has a linear relationship with logarithm of thrombin concentration in the range of 1 fM to 10 pM with a detection limit of 0.02 aM. This work not only provides a new strategy for the design and synthesis of high performance and stable ECL indicator, but also opens up a new approach for the development of highly sensitive ECL sensors for biological analysis.
研究领域:
分析化学
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生物化学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
POLYMER JOURNAL
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2021
·Vol. 53
·期 8
·923-935
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IF 3.08
Yu Li-Ming,Luo Zhen,Gong Cui-Ran,Zheng Yang-Qing,Zhou Zhao-Xi,Zhao Hui,Xu Ying
A new binder, poly(2-propenoic acid, 2-methyl-, 3-[(2-aminoethyl) amino]-2-hydroxypropyl ester) (P-HAEAPMA), is synthesized and applied to silicon/graphite (Si/graphite) anodes in lithium-ion batteries. The Si/graphite anode with the binder exhibits good electrochemical stability. Furthermore, the materials on the discarded Si/graphite/P-HAEAPMA electrode can be recycled for use based on water because the P-HAEAPMA binder is easily soluble in water. The discharge capacity and capacity retention rate of the electrode with reused materials are slightly lower than those of the original Si/graphite/P-HAEAPMA electrode. The binder is rich in polar amino and hydroxyl groups, which not only helps to form strong adhesion between the electrode active material and copper foil but also promotes the transport of Li ions. The electrochemical performance of the electrode with the P-HAEAPMA binder is significantly enhanced compared with that of the electrode with the traditional PVDF binder or water-based LA133 binder. After 305 cycles, a specific capacity of 485.0 mA h g −1 and a capacity retention rate of 74.8% are achieved for the Si/graphite/P-HAEAPMA electrode.
研究领域:
材料科学
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电化学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
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IF 4.598
Libo Li,Bainian Chen,Xiaohong Liu,Panao Jiang,Lijun Luo,Xia Li,Tianyan You
Divalent mercury ion (Hg 2+ ) is widely distributed in the ecological environment and harmful to the human body and the environment. Herein, a highly sensitive and selective ‘on–off-on’ electrochemiluminescence (ECL) aptamer sensing method based on the dual signal amplification strategy of a self-enhanced luminophore and ECL resonance energy transfer (ECL-RET) was developed to detect Hg 2+ . In detail, the first ‘switch-on’ state was obtained by the self-enhanced luminophore of Ru(bpy) 3 2+ -doped silica nanoparticle-nitrogen-doped graphene quantum dots ( [email protected] 2 -NGQDs) modified glassy carbon electrode, which significantly amplified the ECL intensity compared with the mixture of [email protected] 2 and NGQDs . Later, the ‘switch-off’ state was induced by gold nanoparticles (AuNPs), which acted the acceptor in the ECL-RET system between AuNPs and [email protected] 2 -NGQDs due to their good spectral overlap. This combination of the self-enhanced luminophore and ECL-RET provided a novel dual signal amplification strategy, which led to a high signal-to-noise ratio and high analysis sensitivity. Finally, upon the addition of Hg 2+ , the formation of a stable T-Hg 2+ -T structure induced the release of AuNPs from the sensing interface and halted the ECL-RET process, resulting in ECL signal recovery (the second ‘switch-on’ state). For the detection of Hg 2+ , the proposed ECL sensor exhibited a wide linear range from 1 × 10 −14 to 1 × 10 −6 M and a highly sensitive detection limit of 3.0 fM. Finally, the feasibility of the developed method in water sample analysis was investigated.
研究领域:
分析化学
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环境科学
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材料科学
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纳米技术
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传感器技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Libo Li,Bainian Chen,Lijun Luo,Xiaohong Liu,Xiaoya Bi,Tianyan You
In the present study, a novel self-enhanced electrochemiluminescence (ECL) aptasensor which combined self-enhanced ECL composite as signal response element and aptamer as the specific recognition element was firstly proposed for the sensitive and selective detection of Hg 2+ . Innovatively, the luminophore Ru(bpy) 3 2+ -doped silica nanoparticles functionalized with 3-aminopropyltriethoxysilane (NH 2 – [email protected] 2 ) and the co-reactant nitrogen doped graphene quantum dots (NGQDs) were bound together to form the self-enhanced ECL composite, NH 2 – [email protected] 2 -NGQDs, by electrostatic adsorption. Satisfactorily, high and stable luminous efficiency of NH 2 – [email protected] 2 -NGQDs was obtained benefited from the short electron transfer distance and low energy loss of self-enhanced-typal ECL composite. For the fabrication of the self-enhanced ECL aptasensor for Hg 2+ detection, the Hg 2+ aptamer, of which one end was connected to NH 2 – [email protected] 2 -NGQDs, and the other end was fixed on glass carbon electrode (GCE) surface through Au–S bond, was served as a bridge. Upon the addition of Hg 2+ , the aptamer was bent due to the formation of thymine-Hg 2+ -thymine (T-Hg 2+ -T) specific structure, which caused the self-enhanced ECL composite was close to the GCE surface. On this basis, a linearly enhanced ECL signal was acquired with the concentration of Hg 2+ in the range of 5.0 × 10 −11 M − 1.0 × 10 −6 M with excellent selectivity , repeatability and stability in 3 min for each assay. In addition, the proposed aptasensor showed satisfying accuracy and practicability for Hg 2+ analysis in tap and canal water verified by the inductively coupled plasma-mass spectrometry (ICP-MS) method.
研究领域:
分析化学
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环境科学
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Qian Long,Hanfeng Cui,Jing Zhang,Qiqi Fan,Ting Huang,Hai Shuai,Xiaocui Yin,Guobing Wei,Huanwen Chen,Hao Fan
Cu 2+ serves as a significant biomarker in various physiological processes. This study presents the development of a novel Ru-UiO-TPEA functionalized metal-organic framework (MOFs) probe for Cu 2+ detection, employing both electrochemiluminescence (ECL) and electrochemical (EC) methods. Employing a dual ligand-assisted energy transfer approach, we synthesized Ru-UiO with optimal ECL and EC properties by adjusting the synthesis ratio of biphenyldicarboxylic acid to Ru(dcbpy) 3 2+ . This was followed by its conjugation with N-(2-aminoethyl)-N, N′, N′-tris(pyridin-2-ylmethyl) ethane-1,2-diamine (AE-TPEA) to form the highly conjugated Ru-UiO-TPEA probe for Cu 2+ detection. As the Cu 2+ concentration increased, the probe exhibited a diminished ECL signal and an enhanced differential pulse voltammetry (DPV) signal. The dual-mode detection method enabled cross-validation of the ECL and EC signals, enhancing accuracy and effectively minimizing errors. The ECL and EC methods had detection limits of 0.26 nM (range: 1 nM to 10 μM) and 0.65 nM (range: 2 nM to 80 μM), respectively. Meanwhile, these two methods can effectively detect Cu 2+ in rat brain microdialysis fluid. Notably, the measured concentrations correlated well with those determined by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), highlighting its potential in biological and clinical bioelectronic sensing applications.
研究领域:
分析化学
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生物化学
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生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jia Yu,Yuanjie Wu,Chaoxi Zeng,Haoren Wu,Gaofeng Jia,Jianhui Ye,Si Qin,Zhonghua Liu,Meng Shi
Dark tea has long been recognized for its health-promoting benefits, attributed to its complex phytochemical composition. However, the specific bioactive compounds responsible for these beneficial effects remain inadequately characterized. This study aimed to explore the impact of dark tea water extract (DTE) on digestive enzyme activity, antioxidant capacity, and glucose-lipid balance. DTE was fractioned into four fractions using gel separation, followed by analysis with high-performance liquid chromatography and quasi-targeted metabolomics. The 30 % ethanol elution (EEA) and 50 % ethanol elution (EEB) fractions showed stronger antioxidant and enzyme inhibition effects compared to the whole DTE. The EEA fraction was rich in 5 catechins and 26 additional phytochemicals, while the EEB fraction contained high levels of caffeine, ECG, and 29 other phytochemicals. Notably, significant correlations were observed between quercetin-3,4′-O-di-β-glucopyranoside and 3-(2-Naphthyl)-L-alanine with digestive enzyme inhibition. Cellular studies revealed the ability of EEA and EEB to reduce lipid accumulation, improve glycolipid metabolism, and alleviate oxidative stress by increasing SOD, CAT, and GSH levels while decreasing MDA and ROS in HepG2 cells. Furthermore, 34 flavonoids, 2 alkaloids, 2 terpenes, 2 alcohols and polyols, 2 phenylpropanoids and polyketides, 1 organoheterocyclic compound were directly linked to the antioxidant activity and the modulation of glucose and lipid levels. These findings offer valuable insights into the phytochemical profiles of dark tea and its potential health benefits.
研究领域:
食品科学
·
营养学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）