MOLECULES
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2024
·Vol. 29
·期 7
·1534
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IF 4.2
Zhizhi Chen,Lei Wang,Dongxia He,Qi Liu,Qinqin Han,Jinyang Zhang,A-Mei Zhang,Yuzhu Song
Antibiotic resistance has emerged as a grave threat to global public health, leading to an increasing number of treatment failures. Antimicrobial peptides (AMPs) are widely regarded as potential substitutes for traditional antibiotics since they are less likely to induce resistance when used. A novel AMP named Brevinin-1BW (FLPLLAGLAASFLPTIFCKISRKC) was obtained by the Research Center of Molecular Medicine of Yunnan Province from the skin of thePelophylax nigromaculatus. Brevinia-1BW had effective inhibitory effects on Gram-positive bacteria, with a minimum inhibitory concentration (MIC) of 3.125 μg/mL againstEnterococcus faecalis(ATCC 29212) and 6.25 μg/mL against bothStaphylococcus aureus(ATCC 25923) and multidrug-resistantStaphylococcus aureus(ATCC 29213) but had weaker inhibitory effects on Gram-negative bacteria, with a MIC of ≥100 μg/mL. Studies using scanning electron microscopy (SEM) and flow cytometry have revealed that it exerts its antibacterial activity by disrupting bacterial membranes. Additionally, it possesses strong biofilm inhibitory and eradication activities as well as significant lipopolysaccharide (LPS)-binding activity. Furthermore, Brevinin-1BW has shown a significant anti-inflammatory effect in LPS-treated RAW264.7 cells. In conclusion, Brevinin-1BW is anticipated to be a promising clinical agent with potent anti-Gram-positive bacterial and anti-inflammatory properties.
研究领域:
微生物学
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药理学
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传染病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2021
·Vol. 10
·期 3
·834-845
·
IF 6.843
Xiaodan Wang,Fangping Yang,Huawei Yang,Xu Zhang,Haoyu Tang,Shifang Luan
Antimicrobial peptides (AMPs) are attractive antimicrobial agents used to combat bacterial infections, and have been advanced to be one of the most promising alternatives to conventional antibiotics. They stand out for their attractive broad-spectrum activity, unmatched antibacterial mechanism that is not prone to develop drug resistance and diversified topologies, which can be fabricated with manifold amino acid blocks. In this study, using n-hexylamine and amine-terminated polyamidoamine dendrimers (Gx-PAMAM, x = 1–2) as initiators, a series of AMPs with linear and star-shaped topological structures were constructed via the controllable ring-opening polymerization (ROP) of N-carboxyanhydrides (NCAs). The antibacterial performances of the tailored linear and star-shaped AMPs were comprehensively evaluated in both solution states and surface-bonded states. The results indicated that the star-shaped AMPs exhibited enhanced bactericidal activity against Gram-negative E. coli and similar bactericidal activity against Gram-positive S. aureus when compared with the linear AMPs. It is worth mentioning that star-shaped AMPs demonstrated a significantly faster bactericidal efficiency (completely killed bacteria within 5 min at a concentration of 2 × MIC for S. aureus) than their linear analogues (took 15 min to achieve the same effect). However, when the AMPs were immobilized to the surface, they similarly exhibited superior antibacterial activity and fast bactericidal efficiency towards S. aureus and E. coli in the case of the same surface grafting amount. In addition, both the surfaces grafted with AMPs of different topologies demonstrated favorable biocompatibility in vitro.
研究领域:
生物材料
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生物技术
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微生物学
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高分子化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yu Guo,Rongbo Guo,Xiaoshuang Shi,Shujuan Lian,Qiannan Zhou,Ying Chen,Weifeng Liu,Wei Li
In this study, cellulose-based superabsorbent hydrogel was synthesized from sodium carboxymethyl cellulose (CMC-Na), acrylic acid (AA), and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) to enhance its water absorbency and salt tolerance for soil-conditioning applications in areas suffering from drought and soil salinization. Superabsorbent hydrogels (SHs) were prepared by CMC-Na and AMPS successfully, using chemical graft technology. Structure, morphology, thermal stability , and water absorbency of SHs were deduced. The cellulose-based hydrogel showed a high salt tolerance that the maximum water absorbency reached 604 and 119% in distilled water and saline water, respectively. The swelling behavior in aqueous solvents indicated that the water absorption of hydrogels was improved with the increasing ratio of CMC-Na. All SHs exhibited adsorption of nitrogen with the maximum adsorption of ammonia nitrogen 30 mg·g −1 and the presence of hydrogels could slow down the loss of nutrients in the soil. This study provided a feasible strategy that AMPS was substituted by CMC-Na to synthesize SHs with strong water absorbency and high salt tolerance which could be efficiently applied in agriculture as a soil conditioner.
研究领域:
材料科学
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环境科学
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农业科学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
·
IF 9.965
Yinghui Xu,Yupei Li,Weifeng Zhao,Changsheng Zhao
In this study, carboxymethyl chitosan (CMCS)-based double cross-linked network spheres, are constructed by an emulsion template method. The first crosslinking network comes from the chelation between the CMCS and zinc ions , while the second one is based on the covalent crosslinking of 2-acrylamido-2-methyl-1-propanesulfonicacid (AMPS); and the as-prepared spheres are termed as ECMCS@AMPSs. The emulsion template method endows the ECMCS@AMPSs with large pore area (58.3 m 2 /g) and ultra-high porosity (92.3 %). Thanks to the heparin-mimicking functional groups of –OH, –COO - and -SO 3 - , the ECMCS@AMPSs show excellent anticoagulant properties without the activation of the complement system, contact system and platelets . Compared with the spheres without emulsification , the porous structure of the ECMCS@AMPSs results in a 5.3-fold increase in low-density lipoprotein (LDL) adsorption capacity in hypercholesterolemia plasma. Most importantly, in vitro simulated hemoperfusion experiment shows that the cumulative adsorption capacity of LDL by the ECMCS@AMPSs is as high as 41.66 mg/g in 1 h, which is approximately 4.2 times that of high-density lipoprotein (HDL). In conclusion, the ECMCS@AMPSs with self-anticoagulant and high-efficiency adsorption of LDL fabricated via simple emulsion template method have great clinical application prospects in the field of lipoprotein apheresis.
研究领域:
生物医学工程
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材料科学
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药剂学
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产品关联
DOI
PubMed
高影响因子
Insect Science
·
2024
·
IF 2.9
Wen-Xuan Qi,Feng Liu,Fang-Fang Liu,Hai-Yan Ren,Bang-Xian Zhang,Xiao-Qiang Yu,Xiang-Jun Rao
Antimicrobial peptides (AMPs) are critical components of innate immunity in diverse organisms, including plants, vertebrates, and insects. This study identified and characterized a novel Lepidoptera-specific AMP, named lepidoptin, from the invasive pest Spodoptera frugiperda (Lepidoptera: Noctuidae). Lepidoptin is a 116-amino acid protein containing a signal peptide and a novel β -sandwich domain that is distinct from previously reported AMPs. Temporal and spatial expression analyses revealed a significant upregulation of the lepidoptin gene in vivo and in cultured SF9 cells in response to pathogens. Molecular docking analysis identified a specific binding cavity. Enzyme-linked immunosorbent assay and binding assays confirmed that lepidoptin can bind to pathogen-associated molecular patterns, bacteria, and fungi. Recombinant lepidoptin exhibited potent antibacterial activity by inducing bacterial agglutination, inhibiting bacterial growth, increasing bacterial membrane permeability, and preventing biofilm formation. Lepidoptin also showed antifungal activity against the entomopathogenic fungus Beauveria bassiana by inhibiting spore germination, increasing fungal cell permeability, and increasing reactive oxygen species. Injection of recombinant lepidoptin into S. frugiperda larvae increased survival after B. bassiana infection, whereas knockdown of lepidoptin by RNA interference decreased larval survival. In addition, lepidoptin showed antimicrobial activity against the plant pathogen Fusarium graminearum by inhibiting spore germination and alleviating disease symptoms in wheat seedlings and cherry tomatoes. This study demonstrates the remarkable dual functionality of lepidoptin in enhancing S. frugiperda immunity and controlling plant pathogens, making it a promising candidate for biocontrol strategies in both pest management and plant disease prevention.
研究领域:
昆虫学
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微生物学
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分子生物学
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植物病理学
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害虫管理
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生物防治
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
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2021
·
IF 5.64
Wang Zhihua,Li Qiuke,Li Jinze,Li Jiawei,Shang Lu,Chou Shuli,Lyu Yinfeng,Shan Anshan
Antibiotic resistance is emerging as a hot issue with the abuse and overuse of antibiotics, and the shortage of effective antimicrobial agents against multidrug resistant bacteria creates a huge problem to treat the threatening nosocomial skin and soft tissue infection. Antimicrobial peptides (AMPs) exhibite enormous potential as one of the most promising candidates of antibiotic to fight against pathogenic infections because of its unique membrane penetration mechanism to kill pathogens, whereas the clinical application of AMPs still faces the challenges of production cost, stability, safety, and design strategy. Herein, a series of Trp-rich peptides was designed following the principle of paired Trp plated at the ith and ith+4 position on the backbone of peptides, based on the template (VKKX)4, where X represents W, A, or L, to study the effect of intramolecular aromatic interactions on the bioactivity of AMPs. Through comparing the antimicrobial performance, hemolysis, cytotoxicity, and stability, VW5 which is equipped with the characters of direct antimicrobial efficacy (GM=1.68μM) and physical destruction of bacterial membrane (SEM and electron microscopy) stood out from the engineering peptides. VW5 also performed well in mice models, which could significantly decrease the bacterial colony (VW5 vs infection group, 12.72±2.26 vs 5.52±2.01×109CFU/abscess), the area of dermo-necrosis (VW5 vs infection group, 0.74±0.29 vs 1.86±0.98mm2) and the inflammation cytokine levels at the abscess site without causing toxicity to the skin. Overall, this study provides a strategy and template to diminish the randomness in the exploration and design of novel peptides.
研究领域:
药理学
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微生物学
·
传染病学
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分子生物学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Peng Zhou,Xiangzhou Li,Zhi Jiang,Jun Zhou,Guang He,Limin Qu
In this paper, a kind of superabsorbent resin (SAR) with superior quality for hygiene products was developed using Fructus Aurantii Immaturus pectin (FAIP) from Citrus aurantium L.. FAIP-g-AM/AMPS SAR was established by free radical graft co -polymerization with FAIP as skeleton structure, N, N′-Methylene-bis (acrylamide) (MBA) as the cross-linker. Meanwhile, the functional monomers of acrylamide (AM) and 2-acrylamido-2-methylpropane sulfonic acid (AMPS) were introduced. The structure and morphology of FAIP-g-AM/AMPS were characterized by FTIR , 13 C NMR, XRD, SEM and TG-DSC analysis. The results confirmed that the AFIP-g-AM/AMPS SAR was successfully prepared, which exhibited a three-dimensional (3D) network structure and an excellent thermal stability . The absorption and retention capacity of FAIP-g-AM/AMPS was comparable to or even better than commercial diapers and sanitary napkins. Significantly, FAIP-g-AM/AMPS itself exhibited excellent antibacterial and safety. FAIP-g-AM/AMPS has an inhibition ratio of 97.1 % for Escherichia coli ( E. coli ) and 98.5 % for Staphylococcus aureus ( S. aureus ), and was non-irritating and non-allergic to the skin. In addition, FAIP-g-AM/AMPS presented amazing biodegradability and a weight loss reached 37.1 % after 30 days by soil burial test. The research provides a safe and high-performance SAR, which expected to be used in hygiene products such as baby diapers, adult incontinence pads and sanitary napkins.
研究领域:
材料科学
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高分子化学
·
生物技术
·
环境科学
·
卫生用品
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）