FOOD CHEMISTRY
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2022
·
IF 9.231
Xiaoxiang Gao,Chenbo Yue,Ruocen Tian,Leilei Yu,Fengwei Tian,Jianxin Zhao,Wei Chen,Qixiao Zhai
We examined the microbial regulatory capacity of four polyphenols with different structure in healthy mice and explore the mechanism according to exogenous metabolites and microbial metabolites. Oral administration of four polyphenols, including caffeic acid (CA), procyanidin (PA), puerarin (Pue), and resveratrol (Res), did not lead to metabolic disorder in healthy mice. Gut microbiota analysis revealed that CA, PA, and Pue administration significantly enhanced the abundance of Akkermansia and Ruminococcaceae UCG-014 while Res supplement mainly promoted the growth of Lactobacillus and Bacteroides . Furthermore, correlation analysis and exogenous metabolite prediction revealed that the effects of polyphenols, including CA, PA, and Pue, on Akkermansia have strong relationship with uridine while the regulation of Res on microbiota might be dependent on the decrease on petroselinic acid. These investigations considerably suggest the importance of exploration of exogenous metabolites and reveal the similarity of effects of polyphenols on microbiota and metabolites.
研究领域:
微生物学
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营养学
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代谢组学
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DOI
PubMed
高影响因子
GENE EXPRESSION PATTERNS
·
2022
·
IF 1.488
Jian Zhou,Yan-Jing Yang,Rui-Hai Gan,Yang Wang,Zhi Li,Xiao-Juan Zhang,Jian-Fang Gui,Li Zhou
Foxl2 plays conserved central function in ovarian differentiation and maintenance in several fish species. However, its expression pattern and function in fish embryogenesis are still largely unknown. In this study, we first presented a sequential expression pattern of zebrafish foxl2a and foxl2b during embryo development. They were predominantly expressed in the cranial paraxial mesoderm (CPM) and cranial venous vasculature (CVV) during somitogenesis and subsequently expressed in the pharyngeal arches after 48 h post-fertilization (hpf). Then, we compared the brain structures among zebrafish wildtype (WT) and three homozygous foxl2 mutants ( foxl2a −/− , foxl2b −/− and foxl2a −/− ; foxl2b −/− ) and found the reduction of the fourth ventricle in the three foxl2 mutants, especially in foxl2a −/− ; foxl2b −/− mutant. Finally, we detected several key transcription factors involved in the gene regulatory network of midbrain-hindbrain boundary (MHB) patterning, such as wnt1 , en1b and pax2a . Their expression levels were obviously downregulated in MHB of foxl2a −/− and foxl2a −/− ; foxl2b −/− mutants. Thus, we suggest that Foxl2a and Foxl2b are involved in MHB and the fourth ventricle development in zebrafish. The current study provides insights into the molecular mechanism underlying development of brain ventricular system.
研究领域:
发育生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL ECOLOGY
·
2026
·Vol. 52
·期 4
·58
·
IF 2.1
Ma Qianwen,Li Qin,Tan Tingting,Meng Yifei,Wang Hongquan,Chen Yiqu,Tang Xiaoqin
Quercus aquifolioides is an ecologically important oak species on the Qinghai–Tibet Plateau that experiences significant damage from Soritia leptatina . To clarify the behavioral response of S. leptatina to leaf volatiles of Q. aquifolioides , we analyzed leaf-associated volatile organic compounds using solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME–GC–MS), measured electrophysiological and behavioral responses of adult S. leptatina to selected leaf volatiles using electroantennography (EAG) and Y-tube olfactometer assays, and evaluated the field-trapping potential of bioactive volatiles. The detected leaf-associated profile included C6 green-leaf-volatile derivatives, long-chain fatty acids and fatty-acid esters, terpenes, and aromatic compounds. Unmated male and female adults showed strong EAG responses to leaf acetate, palmitic acid, and myristic acid, with the highest response recorded for 10 µg/µL leaf acetate (0.97 mV). Y-tube assays indicated that leaf acetate, myristic acid, and naphthalene elicited significant behavioral attraction at specific concentrations ( P < 0.05), whereas palmitic acid did not show significant attraction in this assay. Field trapping experiments further showed that palmitic acid and myristic acid increased adult captures, with 100 µg/µL myristic acid producing the highest catch (137 individuals). These results suggest that myristic acid and palmitic acid may be useful for monitoring, early warning, and environmentally friendly management of S. leptatina , although their behavioral roles should be interpreted according to assay type and concentration.
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Lu He,Tong Wu,Chongshi Xie,Yue Ma,Boheng Zhang
In this study, ZP was synthesized via a straightforward ion exchange reaction and utilized as both an acid source and a gas source for the preparation of intumescent flame-retardant vinyl ester resin (VER). The chemical structure of ZP was characterized using Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). The flame-retardant performance of the composites was systematically evaluated through limiting oxygen index (LOI), vertical burning (UL-94), and cone calorimetry (CC) tests. The results demonstrated that the VER-ZP17.5 composite achieved an LOI value of 29.6 % and attained a V-0 rating in the UL-94 test. Furthermore, the peak heat release rate (PHRR) and total heat release (THR) were reduced by 32.7 % and 35.7 %, respectively. Analysis and discussion of the flame-retardant mechanism revealed that ZP effectively promoted the formation of an intumescent char layer, significantly inhibiting flame propagation and reducing heat transfer to the internal matrix during combustion. This study provides new insights and experimental evidence for the development of high-performance intumescent flame-retardant composites.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
Neural Regeneration Research
·
2024
·Vol. 20
·期 4
·1164
·
IF 5.9
Wang Pengfei,Bi Yuewei,Li Min,Chen Jiazhi,Wang Zhuyong,Wen Huantao,Zhou Ming,Luo Minjie,Zhang Wangming
Long-term levodopa administration can lead to the development of levodopa-induced dyskinesia. Gamma oscillations are a widely recognized hallmark of abnormal neural electrical activity in levodopa-induced dyskinesia. Currently, studies have reported increased oscillation power in cases of levodopa-induced dyskinesia. However, little is known about how the other electrophysiological parameters of gamma oscillations are altered in levodopa-induced dyskinesia. Furthermore, the role of the dopamine D3 receptor, which is implicated in levodopa-induced dyskinesia, in movement disorder-related changes in neural oscillations is unclear. We found that the cortico-striatal functional connectivity of beta oscillations was enhanced in a model of Parkinson’s disease. Furthermore, levodopa application enhanced cortical gamma oscillations in cortico-striatal projections and cortical gamma aperiodic components, as well as bidirectional primary motor cortex (M1) ↔ dorsolateral striatum gamma flow. Administration of PD128907 (a selective dopamine D3 receptor agonist) induced dyskinesia and excessive gamma oscillations with a bidirectional M1 ↔ dorsolateral striatum flow. However, administration of PG01037 (a selective dopamine D3 receptor antagonist) attenuated dyskinesia, suppressed gamma oscillations and cortical gamma aperiodic components, and decreased gamma causality in the M1 → dorsolateral striatum direction. These findings suggest that the dopamine D3 receptor plays a role in dyskinesia-related oscillatory activity, and that it has potential as a therapeutic target for levodopa-induced dyskinesia.
研究领域:
神经科学
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药理学
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帕金森病
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DOI
高影响因子
ACS Chemical Biology
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2021
·
IF 5.1
Jianrong Liu,Ling Ma,Chang Song,Hui Xing,Shan Cen,Wenhan Lin
Baculiferins are a group of marine sponge-derived polycyclic alkaloids with anti-HIV (human immunodeficiency virus) activities. To identify additional baculiferin-based congeners for SAR analysis and to investigate the mode of action, a total of 18 new baculiferin-type derivatives were synthesized. The inhibitory activities of the congeners against the HIV-1 virus were evaluated in vitro, and the relevant SAR was discussed. Compound 18 exerted the most potent activity toward VSV-G-pseudotyped HIV-1 (IC50 of 3.44 μM) and HIV-1 strain SF33 (IC50 of 2.80 μM) in vitro. To identify the cellular targets, three photoaffinity baculiferin probes were simultaneously synthesized. Photoaffinity labeling experiments together with LC-MS/MS data identified aspartate-tRNA ligase (DARS) as a putative target protein of 18. The overexpression and knockdown of DARS in HEK293T cells provided additional data to demonstrate that DARS is a potential target protein in the regulation of HIV virus infection. The modes of antiviral baculiferins 13 and 18 binding to DARS were determined by a molecular docking simulation. Thus, baculiferin 18 is considered a promising lead as a new molecular target for the development of anti-HIV agents.
研究领域:
药理学
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病毒学
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化学
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分子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Ren Lei,Li Hongdan,Du Jianxiu
Black phosphorus quantum dots (BP QDs) with small size are synthesized using an easy to operate thermal method. It was found that BP QDs possess oxidase-mimicking activity. They can catalyze the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine to produce a blue-colored product even in the absence of hydrogen peroxide. Active oxygen species are proved to be involved in the reaction through the experiments of radical scavenging and electron spin resonance. Biothiols including reduced glutathione and cysteine inactivate the oxidase-mimicking activity of BP QDs, concomitant to the fading of the blue solution. This provides the base for a colorimetric method for the determination of glutathione and cysteine. The decreased absorbance at 652 nm displays linear response to the concentrations of glutathione ranging from 0.1 to 5.0 μmol L −1 , and cysteine from 0.1 to 10.0 μmol L −1 . The detection limits are 0.02 μmol L −1 and 0.03 μmol L −1 for glutathione and cysteine, respectively. Successive determinations of 1.0 μmol L −1 glutathione and 5.0 μmol L −1 cysteine solution give relative standard deviations of 0.8% and 1.7% ( n = 11), respectively. As a preliminary application, the practicability of the method was evaluated by the determination of glutathione in pharmaceutical preparations. This work not only discovers a useful oxidase mimics but also sets up a reliable platform based on BP QDs in colorimetric detection.
Schematic representation of colorimetric determination for biothiols through inactivating oxidase mimetic−like catalytic activity of black phosphorus quantum dots (BP QDs) on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with dissolved oxygen to produce its blue oxidized product (oxTMB).
研究领域:
材料科学
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分析化学
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生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
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2012
·
IF 5.248
Yu-Ting Lee,Chang-Jun Cui,Eve W. L. Chow,Nason Pue,Thierry Lonhienne,Jian-Guo Wang,James A. Fraser,Luke W. Guddat
The sulfonylurea herbicides exert their activity by inhibiting plant acetohydroxyacid synthase (AHAS), the first enzyme in the branched-chain amino acid biosynthesis pathway. It has previously been shown that if the gene for AHAS is deleted in Candida albicans, attenuation of virulence is achieved, suggesting AHAS as an antifungal drug target. Herein, we have cloned, expressed, and purified C. albicans AHAS and shown that several sulfonylureas are inhibitors of this enzyme and possess antifungal activity. The most potent of these compounds is ethyl 2-(N-((4-iodo-6-methoxypyrimidin-2-yl)carbamoyl)sulfamoyl)benzoate (10c), which has a Ki value of 3.8 nM for C. albicans AHAS and an MIC90 of 0.7 μg/mL for this fungus in cell-based assays. For the sulfonylureas tested there was a strong correlation between inhibitory activity toward C. albicans AHAS and fungicidal activity, supporting the hypothesis that AHAS is the target for their inhibitory activity within the cell.
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DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2020
·
IF 4.545
Zhishen Xie,Jianping Zhao,Hui Wang,Yali Jiang,Qiaoling Yang,Yu Fu,Huahui Zeng,Christian Hölscher,Jiangyan Xu,Zhenqiang Zhang
This study aims to investigate whether magnolol (MG), a natural neolignane compound, can prevent AD induced by beta-amyloid (Aβ) and the possible mechanisms involved. MG dose-dependently reduces Aβ deposition, toxicity and memory impairment caused by Aβ in transgenic C. elegans . More importantly, these effects are reversed by GW9662, a selective peroxisome proliferator-activated receptor-γ (PPAR-γ) antagonist. MG is more effective in enhancing PPAR-γ luciferase levels than honokiol (HK). Meanwhile, MG has the potential to bind with the ligand binding domain of PPAR-γ (PPAR-γ-LBD). As expected, MG inhibited the luciferase activity of NF-κB and its target genes of inflammatory cytokines, and this effect was blocked by GW9662. The luciferase activity of Nrf2-ARE expression can be activated by MG and decreased Aβ-induced reactive oxygen species (ROS). The target gene LXR of PPAR-γ is activated by MG, which upregulates ApoE and promotes microglia phagocytosis and the degradation of Aβ, and these effects were also reversed by GW9662. In summary, MG can attenuate Aβ-induced AD and the underlying mechanism is the reduction of inflammation and promotion of phagocytosis and degradation of Aβ, which is dependent on PPAR-γ.
研究领域:
神经科学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Hui Zhang,Chengwen Wang,Wenjian Yue,Xuesong Wang
With the rapid development of tight and unconventional oil and gas resources, maintaining cement sheath integrity is crucial for ensuring zonal isolation and wellbore safety. Conventional cement systems are prone to cracking under downhole conditions, which compromises their sealing capacity. This study develops novel self-healing particles specifically designed for oil well cement that exhibit excellent thermal stability and expand significantly when exposed to methane. A series of experiments demonstrated that the self-healing mechanism involves two distinct patterns: “single-point expansion” and “spreading adhesion”, which effectively seal cracks under hydrocarbon stimulation. The particles showed good compatibility with cement slurry and common additives, enabling a practical self-healing cement formulation. Field applications in two pilot wells confirmed smooth mixing and trouble-free cementing operations. These findings offer both theoretical insights and technical support for the use of hydrocarbon-responsive self-healing cement systems, thereby enhancing long-term sealing performance in challenging downhole environments.
研究领域:
石油工程
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材料科学
·
井筒建造
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智能材料
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DOI
高影响因子
ARCHIVES OF VIROLOGY
·
2016
·Vol. 161
·期 8
·2197-2205
·
IF 2.255
Shi Xiaodan,Chen Zijian,Tang Shengjie,Wu Fei,Xiong Sidong,Dong Chunsheng
Autophagy is an intrinsic cellular process that can degrade cytoplasmic components. It has been reported that several pathogens hijack this process to facilitate their replication. Coxsackievirus B3 (CVB3), a member of the family Picornaviridae , induces autophagy upon infection. However, the details of CVB3-induced autophagy remain a subject of debate. This study applied a combination of multiple assays for the measurement of autophagy and demonstrated that CVB3 induces a complete autophagic flux. Experiments with infected HEK293A cells revealed that autophagosomes were induced upon CVB3 infection. Most of these autophagosomes were mCherry positive in mCherry-GFP-LC3 cells. Conversely, mCherry-positive autophagosomes were rescued to green positive when treated with the acidification inhibitors chloroquine (CQ) and bafilomycin A1 (BAF), suggesting that autophagosomes fused with late endosomes or lysosomes. The co-localization of LC3-positive puncta with lysosome-associated membrane protein 1 (LAMP1) or LysoTracker confirmed that the autophagosomes fused primarily with lysosomes. Interestingly, the disruption of autophagosome formation by 3-methyladenine (3-MA) or ATG5 siRNA treatment during viral infection significantly decreased CVB3 replication. However, inhibitors of lysosomal acidification, fusion, or degradation did not affect viral replication. Therefore, autolysosomes may not be critical for viral replication in vitro .
研究领域:
病毒学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
REDOX REPORT
·
2021
·
IF 4.412
Nabila Jahan,Apu Chowdhury,Ting Li,Ke Xu,Fen Wei,Sicen Wang
Background Progression of Benign Prostate hyperplasia (BPH) is vulnerable to oxidative stress (OS) and prostatic enlargement among the aging males through apoptosis deregulation. Our present study aimed to investigate the effect of neferine (NF) in the regulation of oxidative stress and apoptosis in human BPH-1 cells.Methods BPH epithelial cell line BPH-1 was treated with NF for 24 and 48 h. To measure oxidative stress (OS) we investigated MDA, SOD, and GST expression along with Nrf2 and its downstream gene and protein expression. Cell proliferation and apoptosis regulation was assayed with respective methods.Results Investigation revealed NF remarkably activate Nrf2 and its downstream proteins HO-1 and NQO1 at 48 h more substantially. Nrf2/Keap1 relative gene and protein expression indicated that NF might trigger Nrf2 upregulation by decreasing Keap1 expression. Both NF concentrations (3 µM and 9 µM) were able to deplete ROS and lipid peroxidation, concurrently, up-regulated SOD and GST. NF reduced cell proliferation significantly along with the regulation of apoptotic proteins Bax, Bcl2, Cyt-C, Caspase 9, and Caspase 3 at the same time (48 h).Conclusion This study is the first to manifest that NF may potentially regulate BPH by counterbalancing between OS and apoptosis through the activation of Nrf2-ARE pathway.
研究领域:
药理学
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泌尿学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
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