Frontiers in Marine Science
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2022
·
IF 5.247
Chen Shanji,Tian Danmei,Wei Jihua,Li Cong,Ma Yihan,Gou Xiaoshuang,Shen Yiran,Chen Mei,Zhang Sihao,Li Jia,Wu Bin,Tang Jinshan
Two new citrinin dimers bearing a 6,6-spiroketal moiety (1 and 2) and four known analogues (3–6), together with 18 known citrinin monomers (7–24), were isolated from the culture of hydrothermal vent-associated fungus Penicillium citrinum Y34. Their structures were identified by extensive spectroscopic analyses, 13C NMR calculation in combination with DP4+, linear correlation coefficient (R2), and mean absolute error (MAE) values analyses, and electronic circular dichroism (ECD) calculation. The α-glucosidase and ATP-citrate lyase (ACL) inhibitory activities of isolated compounds were evaluated. Compounds 1, 3, and 12 displayed moderate α-glucosidase inhibitory activities with IC50 values of 239.8, 176.2, and 424.4 μM, respectively. Enzyme kinetics investigations of 1 and 3 suggested their non-competitive inhibition of α-glucosidase with Ki values of 204.3 and 212.7 μM, respectively. Meanwhile, compound 4 showed significant ACL inhibitory potential with an IC50 value of 17.4 μM. Furthermore, the interactions of 1, 3, and 12 with α-glucosidase and 4 with ACL were investigated by molecular docking assay. This study demonstrates that citrinins, especially for their dimers, could be potential lead compounds for the development of new agents for the treatment of metabolic diseases.
研究领域:
药理学
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生物化学
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代谢疾病研究
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DOI
高影响因子
RSC Advances
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2022
·Vol. 12
·期 46
·30024-30029
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IF 4.036
Baozhu Zhang,Ziyao Yang,Yuxia Li,Ling Ma,Fenfang Li,Xiuqing Lv,Guangming Wen
A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.
研究领域:
分析化学
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生物传感器
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纳米技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Yuanyuan Su,Minghui Gu,Cheng Li,Dequan Zhang,Yuqing Ren,Li Chen,Shaobo Li,Xiaochun Zheng
Adenosine triphosphate (ATP) plays a vital role in physiological processes and is an essential indicator of microbial content in food. Herein, a new sensitive, rapid and water-soluble probe for ATP detection was developed. Rhodamine B and pentaethylenehexamine were employed to design and synthesise the probe rhodamine-pentaethylenehexamine (RP) for selective ATP detection. The synthesised probe RP was characterized using Fourier transform infrared, NMR and dynamic light scattering size distributions. Upon the addition of ATP, the probe exhibited a distinct change in fluorescence intensity, with fluorescence emission at 580 nm. A linear relationship was observed between fluorescence intensity and ATP concentrations at 0–50 μmol/L, with a limit of detection of 10.97 × 10 −9 mol/L. The results of the zeta potential and molecular dynamics simulation demonstrated that the detection mechanism of the probe RP is associated with the electrostatic adsorption interaction between the multi-positively charged sites of RP and the negatively charged triphosphate structure of ATP. Our study provides new insights into improving charge site identification in small molecule detection. Furthermore, the successful detection of ATP on meat surfaces indicates that RP has the potential to assess meat freshness.
研究领域:
分析化学
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食品科学
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生物技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Shengjie Jiang,Jiabin Qiu,Shibing Chen,Hongyu Guo,Fafu Yang
Although all kinds of sensors with unique detecting ability for one guest were reported, the fluorescence sensor with multiple detecting abilities was seldom presented. This work designed and synthesized a novel AIE fluorescence probe bearing double detecting for ATP based on Cu 2+ and Zn 2+ response of hydrazono-bis-tetraphenylethylene ( Bis-TPE ). Bis-TPE was prepared in 82% yield with simple procedure. It exhibited strong red AIE fluorescence based on the large conjugated electron effect in aqueous media. It showed outstanding selective sensing abilities for Cu 2+ by strong fluorescence quenching and for Zn 2+ by red-orange fluorescence change. The sensing mechanism of 1:1 stoichiometric ratios was confirmed by 1 H NMR and MS study. The strong red fluorescence of Bis-TPE + Cu 2+ system could be recovered by adding ATP. The orange fluorescence of Bis-TPE + Zn 2+ system could be quenched by adding Cu 2+ and then was recovered by adding ATP. These double detecting abilities for ATP with the “off-on” red fluorescence in Bis-TPE + Cu 2+ system and “allochroic-off-on” orange fluorescence in Bis-TPE + Zn 2+ +Cu 2+ system were successfully applied to test Cu 2+ , Zn 2+ and ATP in test paper and living cell imaging, displaying the good application prospects for sensing Cu 2+ , Zn 2+ and double detecting ATP in the complicated environment.
研究领域:
分析化学
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生物化学
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材料科学
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生物成像
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
De-Yan Qi,Chong Wang,Yan-Cai Gao,Hong-Wei Li,Yuqing Wu
The glutathione-capped bimetallic copper and gold nanoclusters (CuAuNCs@GSH) were achieved through Au doping into the unstable copper nanoclusters (CuNCs@GSH), which induced the photoluminescence stabilization time increase from 4 to 7 days. Moreover, the Ce 3+ introduction further significantly increased the stable storage time (from 7 to 25 days) and the emission intensity (near 140-fold), accompanying a quantum yields enhancement from 0.30% to 22%. Transmission electron microscopy (TEM) revealed the Ce 3+ -induced larger assemblies of CuAuNCs-Ce 3+ from monodispersive CuAuNCs@GSH nanodots . The fluorescence enhancement was attributed to the aggregation-induced emission (AIE) property of CuAuNCs@GSH, where Ce 3+ was bound to the ligands GSH and reduced the non-radiative relation of the excited states. Besides, the strong interaction between ATP and Ce 3+ dissociated the CuAuNCs-Ce 3+ assembly into monodisperse nanoclusters and recovered the weak luminescence. Therefore, the CuAuNCs-Ce 3+ assembly was applied to determine ATP, which exhibited high selectivity and sensitivity with a detection limit of 53 nM. Finally, the method was successfully applied to determine ATP in diluted serum with very high recovery. Therefore, the present study provides an efficient way to improve the fluorescence properties of metal nanoclusters and supplies a selective and sensitive ATP sensor.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Ting Yu,Yang Li,Jing Li,Yabing Gan,Zhengze Long,Yun Deng,Youyu Zhang,Haitao Li,Peng Yin,Shouzhuo Yao
Adenosine triphosphate (ATP) is a critical intracellular energy currency that plays a key role in various cellular processes and is closely associated with numerous diseases. Similarly, biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are integral to many physiological and pathological processes due to their strong redox properties. Simultaneous discrimination and detection of ATP and biothiols offer valuable insights into the pathogenesis of conditions such as epilepsy and liver injury. This study introduces the first fluorescent probe, BCR , designed for multifunctional detection of ATP, GSH, Hcy, and Cys. With outstanding optical properties, excellent biocompatibility, high selectivity, and superior sensitivity, probe BCR enables effective imaging of ATP and biothiol dynamics in vivo. Moreover, probe BCR successfully visualizes changes in ATP, GSH, Hcy, and Cys levels in a PTZ-induced epileptic zebrafish model and an APAP-induced mouse liver injury tissue section model. These findings underscore the significant potential of probe BCR for early disease diagnosis and therapeutic applications.
研究领域:
生物化学
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医学诊断
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药理学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yu Zhang,Runjie Miao,Wenyan Ma,Haifeng Sha,Hangrong Chen
Aggregation-induced emission (AIE) provides a new direction for fluorescence, but dyes with AIE properties are mostly organic molecules with poor water solubility. Herein, a novel dual fluorescence emitting nanoprobe (named as CCZ) was developed by co-loading blue emitting copper nanoclusters (CuNCs) and yellow emitting curcumin (CUR) into ZIF framework for visual detection of ATP. The prepared CuNCs are featured with good water solubility, bright blue fluorescence, and AIE property. After encapsulation within ZIF-8, CuNCs@ZIF can obviously increase the fluorescence quantum yield (10.15%), fluorescence intensity (4.29 times that of pure CuNCs), and fluorescence stability (only 10% fluorescence decay after seven days sunlight). Based on the stronger coordination between ATP and Zn 2+ , a novel dual fluorescence responsive probe CCZ was constructed by loading curcumin to perform ratio fluorescence combined with colorimetric dual-mode detection of ATP, with the limit of detection (LOD) of 7.5 μM and 0.2 μM, respectively, presenting high specificity for ATP. More interestingly, it could achieve naked eye semi-quantitative sensing of ATP based on the color change, showing great potentials for application in the detection of real samples.
研究领域:
分析化学
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生物传感
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纳米医学
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分子生物学
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化学生物学
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诊断学
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产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2016
·Vol. 14
·期 9
·2684-2690
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IF 3.559
Kui Wang,Jian-Hua Cui,Si-Yang Xing,Hong-Xi Dou
We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence “switch-off” assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.
研究领域:
生物化学
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分析化学
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诊断学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 145
·期 2
·445-452
·
IF 4.019
Yanfang Cui,Haiwang Wang,Su Liu,Yu Wang,Jiadong Huang
The self-assembly of plasmonic nanoparticles provides a powerful approach to generate surface-enhanced Raman scattering (SERS), which promotes the actual applications in chemical and biomolecular analyses. Herein, we developed a facile SERS sensing strategy for an ATP assay with a 3-D DNA nanomachine that walks by the Exo III cleavage, leading to the formation of AuNP aggregates, which resulted in the enhancement of the electromagnetic field. Depending on the target-activated Exo III cleavage, the 3-D nanomachine can walk along the 3-D track on the surface of AuNPs and generate self-assembled hot-spots to enhance the SERS signal of a Raman dye, allowing a homogenous assay of the ATP concentration with high sensitivity and reproducibility. Under optimized experimental conditions, the biosensor detected ATP with a widened dynamic range from 1 pM to 1 × 105 pM with a limit of detection of up to 0.29 pM. Hence, the novel strategy provides a useful and practical platform for the SERS assay of ATP with high sensitivity and repeatability. Besides, this platform shows great potential for applications in high-throughput assays for drug screening and clinical diagnostics.
研究领域:
生物化学
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纳米生物学
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分析化学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Jiabi Xu,Jiayi Peng,Xiangting Wang,Xiaohong Hou
Nanozymes have gradually become a research hotspot in the field of enzymology, benefiting from their low cost, high stability, and availability. To break through the limitation that conventional nanozymes exert peroxidase-like (POD-like) activity under only acidic conditions, we rationally designed Fe3O4@MIL-100(Fe) with excellent superparamagnetism and catalytic activity. An optimization of the microwave-assisted in situ synthesis conditions was carried out to obtain the highest POD-like activity of the nanozyme under nearly neutral conditions. Adenosine triphosphate (ATP) was appointed as an auxiliary regulator to further amplify the activity of the nanozyme Fe3O4@MIL-100(Fe). A series of behaviors related to enzymology and the mechanism of ATP-assisted enhancement were further explored. Consequently, a colorimetric sensing strategy was proposed for the one-step detection of cholesterol with a linear range of 0.05–0.4 mM and a limit of detection (LOD) of 1.3 μM (3.3 δ/S). The result of the present work implied that the established one-step platform could be successfully applied to the detection of cholesterol in actual samples. From the nanozyme synthesis to the colorimetric sensing process, the concepts of time savings and straightforwardness were always upheld and satisfactorily implemented. Most importantly, Fe3O4@MIL-100(Fe) could be a great potential biocatalyst candidate for the development of the corresponding commercial kits.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Cheng Xia,Cen Yao,Xu Guanhong,Wei Fangdi,Shi Menglan,Xu Xiaoman,Sohail Muhammad,Hu Qin
The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via π-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.
Schematic of a novel fluorometric ATP assay based on the fluorescence resonance energy transfer (FRET) between aptamer modified carbon dots (CD-aptamer) and graphene oxide (GO). CD-aptamer was used as the energy donor and molecular recognition probe, and GO acted as energy acceptor. This assay exhibits high sensitivity and selectivity with a detection limit as low as 80 pM.
研究领域:
分析化学
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生物传感器
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Bing-Huan Huang,Zhi-Rong Geng,Xiao-Yan Ma,Cui Zhang,Zhi-Yang Zhang,Zhi-Lin Wang
Lysosomes in astrocytes and microglia can release ATP as the signaling molecule for the cells through ca 2+ -dependent exocytosis in response to various stimuli. At present, fluorescent probes that can detect ATP in lysosomes have not been reported. In this work, we have developed a new water-soluble cationic polythiophene derivative that can be specifically localized in lysosomes and can be utilized as a fluorescent probe to sense ATP in cells. PEMTEI exhibits high selectivity and sensitivity to ATP at physiological pH values and the detection limit of ATP is as low as 10 −11 M. The probe has low cytotoxicity, good permeability and high photostability in living cells and has been applied successfully to real-time monitoring of the change in concentrations of ATP in lysosomes though fluorescence microscopy . We also demonstrated that lysosomes in Hela cells can release ATP through Ca 2+ -dependent exocytosis in response to drug stimuli.
研究领域:
细胞生物学
·
神经科学
·
生物化学
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DOI
PubMed
高影响因子
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