SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Qian Ran,Boyan Sun,Xiaomei Wang,Jiangkang Ren,Chujun Liu,Qiqi Wang,Zimo Liu,Jianzhong Shen,Jincheng Xiong,Haiyang Jiang
s The illicit use of clenbuterol (CLB) in livestock farming poses significant public health risks, necessitating sensitive and reliable detection methods. Conventional gold nanoparticle-based lateral flow immunoassays (AuNPs-LFIAs) are often hampered by limited sensitivity and a narrow dynamic range. Here, we developed a dual-readout nanohybrid probe via an electrostatic integration of AuNPs into metal-AIEgen frameworks (MAFs). The intrinsic fluorescence of MAFs (excitation≈350 nm, emission≈475 nm) exhibits minimal spectral overlap with AuNP absorption (≈520 nm), thereby retaining 78.6% of the MAF's quantum yield while achieving a 2-fold enhancement in colorimetric absorbance. The resulting probe integrates bright fluorescence and intense colorimetric signal into a robust LFIA strip. For CLB detection, the constructed dual-readout LFIA achieved an ultra-wide linear range (0.01–3.90 ng/mL) and a significantly improved detection limit (0.007 ng/mL), demonstrating a 41-fold wider linear range compared to conventional AuNPs-LFIA. The method demonstrated satisfactory recovery rates (87.5–108.3%) in spiked swine urine and pork samples, and showed high consistency with high-performance liquid chromatography-tandem mass spectrometry results when applied to real samples. This work presents a generalizable design principle for spectral-interference-minimal dual-readout probes, advancing LFIA technology with high sensitivity, an ultrabroad dynamic range, and reliable performance for on-site “farm-to-table” monitoring.
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OPTICAL MATERIALS
·
2025
·
IF 4.2
Bing Xu,Liu Yang,Yan Li,Chunyu Cao
A Spermine Oxidase (SMOX)detection local surface plasmon resonance/surface plasmon resonance (SPR/LSPR) optical fiber sensor was constructed with a three-layer structure consisting of gold film/gold nanospheres (AuNPs)/SMOX-antibody in this study. The detection of SMOX was achieved through the specific binding of immobilized antibodies to free antigens in a buffer solution. The AuNPs were immobilized on Gold Film by 1,6-Hexanedithiol (DMH) linker to enhance the sensitivity of optical fiber probe from 1821.21 to 2204.97nm/RIU. The Zeta potential and Fourier Transform Infrared Spectroscopy (FTIR) indicated that the SMOX antibody was attached to the surface of the optical fiber probe by physisorption to construct a SMOX sensor. The SMOX-detection experimental data conformed to the Langmuir model with an R 2 value of 0.92, a lower limit of detection of 0.625μg/ml and exhibited excellent selectivity, high stability, and a rapid response time of 12 min. This method provides a new concept for SMOX detection and has a broad application prospect in the field of biomedical diagnosis.
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高影响因子
BIOMEDICAL MICRODEVICES
·
2026
·Vol. 28
·期 1
·18
·
IF 4.4
Pang Zhengtian,Song Wenqi,Cai Yuanyuan,Li Yahang
Wearable sensors represent a promising platform for the non-invasive and continuous monitoring of biomarkers in biological fluids, showing considerable potential for real-time health management. In this study, we designed a wearable device integrated with gold nanoparticles (AuNPs) for glucose detection. The system features in-situ synthesized AuNPs embedded in a polyacrylamide (PAM) hydrogel—denoted as AuNPs@PAM—enabling dual-mode readout through both visual observation and UV–visible spectrophotometry. By leveraging the dual peroxidase-like and glucose oxidase-like activities of AuNPs, a nanozyme-enzyme cascade catalytic system was constructed for colorimetric glucose sensing, which demonstrated high sensitivity and strong stability. The in-situ synthesis of AuNPs within the PAM matrix enhances nanoparticle stability and increases the reactive surface area, thereby improving catalytic efficiency. Owing to the high flexibility and optical transparency of the PAM hydrogel, the sensing patch enables rapid and reliable glucose detection by both instrumental and visual means. Consequently, a sensitive and visually responsive flexible sensor has been successfully fabricated, offering an effective tool for non-invasive biomarker monitoring in superficial biofluids.
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高影响因子
Results in Chemistry
·
2022
·
IF 0
Han Sun,Haiping Huang,Chao Hu,Yu Yan,Yongmei Hu,Shengda Guo,Jing-Lin Chen
Cu-Ni oxide nanocomposite was synthesized via solvothermal method with nickel (II) nitrate and copper nitrate as Ni and Cu resource respectively. AuNPs/oxide nanocomposite was obtained by dispersing the oxide into the pre-synthesized Au colloid. Transmission electron microscope (TEM) shows that the synthesized oxides are nanoplate morphology. Interplanar distance from the high resolution TEM (HRTEM) image shows that the Ni and Cu in the oxide are multivalent state. X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) characterization further proves that the Ni and Cu are multivalent state, and the Au is Au 0 form in the nanocomposte. EDS-mapping images confirm large numbers of AuNPs are uniformly absorbed onto the ultra-thin multivalent Cu-Ni oxide nanoplate. The electrochemical properties of multivalent oxide (m-v oxide) with or without AuNPs decoration are investigated in 0.1 M potassium chloride electrolyte with 2.0 mM Ferri/Ferro-Cyanide. When employed for the electrochemical catalysis in methanol oxidation reaction (MOR), the AuNPs/m-v oxide exhibits better electrochemistry performance than m-v oxide. The current density of AuNPs/m-v oxide (21.1 mA/cm 2 ) is 3.4 times bigger than that of m-v oxide/GCE (6.2 mA/cm 2 ). What’s more, it shows good electrochemical stability and lower slope of the Tafel plot (41.87 mV/dec).
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Lina Lv,Baozhong Zhang,Panpan Tian,Lingling Xie,Wenhao Wei,Jintao He,Min Lin,Huina Zhu,Hanyu Chen,Baoshan He
In this project, we developed an electrochemical aptamer sensor based on the coordinated catalysis toward thionine (Thi) of nickel metal-organic framework (Ni-MOF) and gold nanoparticles (AuNPs) to sensitively detect the enrofloxacin (ENR), which belonged to a third-generation quinolone antibiotic for treat diseases caused by bacteria. In order to improve the analytical performance of the sensor, Ni-MOF and gold AuNPs, as the substrate material, could increase the load of the aptamer (Apt) and synergistically catalyze thionine (Thi). Therefore, ultrathin sheets of gold triangular nanosheets (AuTNSs) were prepared as signal labeling materials, which were loaded with a large amount of Thi and complementary deoxyribonucleic acid (cDNA). With the help with Ni-MOF and AuNPs catalysis toward Thi, under optimal conditions, the aptasensor exhibited a massive detection range of 1×10 −2 pg·mL −1 to 1×10 3 pg·mL −1 (S/N = 3) and a detection limit of 5.6×10 −3 pg·mL −1 for ENR. Meanwhile, the proposed sensor has a high sensitivity, satisfactory repeatability and long-time stability, and could be well applied to the detection of food samples.
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RSC Advances
·
2022
·Vol. 12
·期 43
·27940-27947
·
IF 4.036
Pengfei Wang,Bin Luo,Ke Liu,Cheng Wang,Hongtu Dong,Xiaodong Wang,Peichen Hou,Aixue Li
Aflatoxin B1 (AFB1), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this study, an electrochemical aptamer sensor was developed for the on-site detection of AFB1. Carboxylated graphene oxide (COOH–GO) and carboxylated multi-walled carbon nanotubes (COOH–MWNT) nanocomposites, dopamine polymers (pDA) and gold nanoparticles (AuNPs) were used to enhance the electrochemical activity and the biocompatibility of the screen-printed electrodes (SPE). Once AFB1 was captured by the aptamer immobilized on the electrode surface, the redox current of [Fe(CN)6]3−/4− decreased. Therefore, the binding of aptamer (Apt) and AFB1 can be reflected by the change of the peak current. The as-prepared sensor showed a wide detection range of 0.1 fg ml−1–100 pg ml−1 and a low detection limit of 15.16 ag ml−1. It is also simple and low-cost, which shows great potential in practical application.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Jing Liu,Ninglong Zhang,Jiansen Li,Mingyang Li,Guangxian Wang,Wenli Wang,Yuxia Fan,Shui Jiang,Gaole Chen,Yin Zhang,Xia Sun,Yuan Liu
The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on [email protected] /Ti 3 C 2 MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of [email protected] and Ti 3 C 2 MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10 −11 –10 −3 M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Wei Song,Yihong Wang
In this work, we report AuNPs-decorated pyrolyzed Co-BDC nanosheets (p-Co-BDC/AuNPs) as high-performance electrocatalyst for developing an electrochemical platform. p-Co-BDC/AuNPs as a new electrocatalyst showed superior electrocatalytic activity towards the electrochemical oxidation of methylene blue (MB). Besides, magnetic p-Co-BDC/AuNPs can be well immobilized on the magnetic glassy carbon electrode without further assistance. The oxidation of MB can be reduced by ascorbic acid . Inspired by this phenomenon, an electrochemical biosensor was constructed based on multiple signal amplification for the diagnosis of miRNAs . Firstly, p-Co-BDC/AuNPs enhanced the electrochemical oxidation of MB. Then, strand displacement amplification reaction can form lots of double helix structure DNA to embed more MB molecules. Finally, ascorbic acid in the electrolyte was utilized to reduce the oxidation of MB and improve the electrochemical signal of MB electro-oxidation. The linear detection range for the detection of miRNAs is 100 aM to 10 nM, and the limit of detection is 86 aM. Furthermore, the constructed biosensor also displayed satisfactory selectivity , good reproducibility, and excellent recovery in the detection of real samples. We are convinced that our proposed multiple signal amplification strategy will provide more promising methods for the diagnosis of cancer.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-9
·
IF 5.833
Wang Lina,Zhang Li,Yu Ying,Lin Bixia,Wang Yumin,Guo Manli,Cao Yujuan
A sensitive electrochemical strategy was established for kanamycin determination. A specific aptamer was modified on the electrode as the probe, followed by a cyclic hybridization chain reaction (HCR) with methylene blue, causing an increasing signal response. In the presence of kanamycin, it can initiatively convolve the aptamer and prevent further DNA assembling, resulting in a signal distinction sensitive to the target amount. However, the signal reproducibility is low. To improve the precision, the HCR procedure was investigated. The results demonstrated that the optimal amount of assembled DNA is 12-fold to that of aptamer. This amount was then controlled in further assays. Admittedly, controlled DNA assembling commonly indicates a limited signal amplification. To further enhance the sensitivity, a nanocomposite based on MoS 2 and AuNPs was modified on the electrode. The results of the assay proved that the signal distinction sensitive to target amount increased by 50%. A linearity range is obtained from 0.01 nM to 1.0 μM of kanamycin, and the LOD is 8.4 pM. Subsequently, this strategy was employed to detect kanamycin in chicken liver and milk sample; the recovery results suggest that it possess a satisfactory application prospect in analysis of agricultural products. Graphical abstract
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Xujie Su,Yujie Han,Zhiguang Liu,Lifang Fan,Yujing Guo
Simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. This paper describes an alternative method for detection of nucleolin, which is a major nucleolar protein, overexpressed in several types of cancer, and is a cancer-specific target. Gold nanoparticles/molybdenum disulfide/reduced graphene oxide (AuNPs/MoS 2 /rGO) was easily synthesized with hydrothermal method and characterized by transmission electron microscopy (TEM), x-ray diffraction (XRD) and x-ray photoelectron spectroscopy (XPS). Combining the properties of large surface area and good electrical conductivity of AuNPs/MoS 2 /rGO with the high affinity and specificity of aptamer, a smart hierarchically self-assembled electrochemical aptasensor was established for the specific and sensitive nucleolin (as a model analyte) detection. Under the optimized conditions, AuNPs/MoS 2 /rGO based aptasensor exhibits a great performance for the detection of nucleolin with a linear response from 0.5 nM to 1.0 μM and a low detection limit of 0.16 nM. In addition, this electrochemical aptasensor has good reproducibility towards nucleolin. Besides, the practicability of the TNA (thiolated nucleolin aptamer)/AuNPs/MoS 2 /rGO was demonstrated by analysis of nucleolin in the blood sample. This work paves a promising way for the facile synthesis and design MoS 2 /rGO nanocomposite-based electrochemical aptasensor for testing tumor markers in clinical diagnostics.
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Chemosensors
·
2022
·Vol. 10
·期 10
·407
·
IF 4.229
Yaqin Chen,Xia Peng,Yonghai Song,Guangran Ma
An electrochemical sensor based on a molecularly imprinted polymer membrane (MIP) was developed. The electrochemical sensor was prepared by electropolymerization of o-phenylenediamine (O-PD) on the surface of glassy carbon electrode (GCE), modified by AuNPs@covalent organic framework (COF) microspheres with ascorbic acid (AA) as template molecule. First, ultrasmall polyvinylpyrrolidone (PVP)-coated AuNPs were prepared by a chemical reduction method. Then, 1,3,5-tri(p-formylphenyl)benzene (TFPB) and N-boc-1,4-phenylene diamine (NBPDA) underwent an ammonaldehyde condensation reaction on PVP-coated AuNPs to form AuNPs@COFTFPB-NBPDAmicrospheres. The porous spherical structure of AuNPs@ COFTFPB-NBPDAcould accelerate the mass transfer, enlarge the specific surface area, and enhance the catalytic activity of PVP-coated AuNPs. The electrochemical sensors, based on AuNPs@ COFTFPB-NBPDA/GCE and nMIPs/AuNPs@COFTFPB-NBPDA/GCE, were applied for the detection of AA, with a detection limit of 1.69 and 2.57 μM, as well as linear ranges of 5.07 to 60 mM and 7.81 to 60 mM. The nMIPs/AuNPs@COFTFPB-NBPDAsensor had satisfactory stability, selectivity, and reproducibility for AA detection.Keywords:covalent organic framework;ascorbic acid;molecularly imprinted polymer;electrochemical sensor
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