Food Bioscience
·
2021
·
IF 4.24
Wen Hedi,Liu Jingbo,Yu Yiding,Sun Yuxi,Liu Jiyun,Deng Qinqin,Chen Yan,Liu Boqun,Zhang Ting
Self-aggregation and the hemolytic effect limit the application of γ-cyclodextrin (γ-CD) in bioactive molecular delivery systems. In this study, γ-CD was modified by grafting onto bovine serum albumin protein (BSA), with epichlorohydrin (ECH) acting as the cross-linking agent. The effects of BSA concentration, reaction temperature, pH and time on the grafting rate were studied, and the γ-CD-BSA complex with a grafting rate of 99.5 ± 0.1% ± 0.06 ) % was achieved. The complex was confirmed using 1 H NMR and FT-IR spectra. Compared with γ-CD, the hemolytic effect and self-aggregation of γ-CD-BSA were significantly reduced, and the encapsulation efficiency of curcumin was increased by 10.8%. The results of scanning electron microscopy showed that both γ-CD and γ-CD-BSA nanoparticles were formed and the structure of the γ-CD-BSA complex was more uniform. The pH stability and salt stability of γ-CD-BSA were higher than γ-CD. The release rate of γ-CD-BSA was 15.2 ± 0.2% after 2 h at pH 1.2, and 57 ± 1% after 4 h at pH 7.2. The γ-CD-BSA nanoparticles could protect curcumin in acidic environments and release it in neutral environments. The results suggested a promising wall material for delivery of hydrophobic substances.
研究领域:
生物化学
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药物递送系统
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Tian Li,Zhengjun Cheng,Lijun Cao,Xiaohui Jiang
Fast Green FCF (FCF), Patent Blue V (PBV) and Acid Blue 1 (AB1) are used as food colorants. Multiple spectroscopic techniques were employed to probe in depth the affinity of FCF/PBV/AB1 with BSA in different pH and/or salt concentrations. The results showed that FCF/PBV/AB1 quenched the intrinsic fluorescence of BSA by a static process, and electrostatic force dominated the formation of BSA–FCF/PBV/AB1 complex which was confirmed by the effects of salt on their interactions. Subdomain IIA was the primary binding site for FCF/PBV/AB1 on BSA in the pH range of 5.5–7.4, while both Trp 212 and Trp 134 residues of BSA might be bound by FCF/PBV/AB1 at pH 4.8. The K values suggested that the binding ability of three food colorants with BSA was FCF > PBV > AB1. The results of UV–vis absorption, synchronous fluorescence, 3D fluorescence and FT-IR spectra proved that the structure of BSA altered by FCF/PBV/AB1.
研究领域:
生物化学
·
食品科学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 133
·期 36
·
IF 1.866
Jiangquan Liu,Xiaoguang Ying,Hongxun Wang,Xiao Li,Weiying Zhang
Polyethylene glycol grafted calcium alginate hydrogel microspheres are synthesized by ring-opening and addition reaction of epoxy chloropropane. FTIR, 13 C NMR, optical microscope, SEM, TG, and DSC are used for characterization. Swelling behaviors are studied and it is found that polyethylene glycol grafted calcium alginate microspheres are improved in anti-swelling properties. Protein-imprinted polyethylene glycol grafted calcium alginate hydrogel microspheres exhibit enhanced specific rebinding quantity (11.16 mg/g) and selective recognition properties (4.71 against BHb), towards target molecule in the presence of competing protein, compared with that of the unmodified samples (4.96 mg/g and 1.75, respectively). © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43617.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
Nanoscale Research Letters
·
2021
·Vol. 16
·期 1
·1-11
·
IF 4.703
Zhao Shubi,Luo Yiqun,Chang Zong,Liu Chenchen,Li Tong,Gan Lu,Huang Yong,Sun Qinchao
The second near infrared window is considered to be the optimal optical window for medical imaging and therapy as its capability of deep tissue penetration. The preparation of the gold nanorods with long wavelength absorption and low cytotoxicity is still a challenge. A series gold nanorods with large aspect ratio have been synthesized. Strong plasma absorption in the second near infrared window from 1000 to 1300 nm could be observed. The biocompatibility of the synthesized gold nanorods is dramatically improved via coating by bovine serum albumin (BSA), while the optical properties of which remains. The breast cancer tumor-bearing mouse could be well treated by the prepared gold nanorods with the NIR-II light intensity as low as 0.75 W/cm2. In summary, these results demonstrate the feasibility of using low illumination dose to treat tumor in the NIR-II region via the large aspect ratio gould nanoparticles.
研究领域:
纳米技术
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生物医学工程
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癌症治疗
·
光学成像
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2009
·Vol. 135
·期 1
·111-115
·
IF 3.761
Shenguang Ge,Congcong Zhang,Yuanna Zhu,Jinghua Yu,Shuangshuang Zhang
A novel fluorescent nanosensor for Sb3+ determination was reported based on thioglycolic acid (TGA)-capped CdTe quantum dot (QD) nanoparticles. It was the first antimony ion sensor using QD nanoparticles in a receptor-fluorophore system. The water-soluable TGA-capped CdTe QDs were prepared through a hydrothermal route, NaHTe was used as the Te precursor for CdTe QDs synthesis. Bovine serum albumin (BSA) conjugated to TGA-capped CdTe via an amide link interacting with carboxyl of the TGA-capped CdTe. When antimony ion enters the BSA, the lone pair electrons of the nitrogen and oxygen atom become involved in the coordination, switching off the QD emission and a dramatic quenching of the fluorescence intensity results, allowing the detection of low concentrations of antimony ions. Using the operating principle, the antimony ion sensor based on QD nanoparticles showed a very good linearity in the range 0.10–22.0 μg L−1, with the detection limit lower than 2.94 × 10−8 g L−1 and the relative standard deviation (RSD) 2.54% (n = 6). In a study of interferences, the antimony-sensitive TGA-QD-BSA sensor showed good selectivity. Therefore, a simple, fast, sensitive, and highly selective assay for antimony has been built. The presented method has been applied successfully to the determination of antimony in real water samples (n = 6) with satisfactory results.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 95
·期 8
·2195-2207
·
IF 2.75
Wei Shi,Si-Qi Zhang,Kai-Bin Li,Ye-Jing Deng,Chen-Yi Wang,Yi-Ting Lu,Jiao-Jiao Liu,De-Man Han
BACKGROUND Hydrophobic interaction chromatography (HIC), as a typical separation technology, often suffers from low selectivity because it has a single interaction mode. Herein, a novel HIC resin (Octyl@BSA) with imprinted layer was synthesized and used for protein adsorption. Bovine serum albumin (BSA) and bovine serum γ-globulin (bIgG) were used as model competitive proteins in this study. The effects of pH, salt type, concentration, and mass ratio were then investigated. RESULTS The data from the static and dynamic adsorption experiments indicated that the introduction of spatial recognition effectively reduced the adsorption of competitive proteins, leading to significant improvements in selectivity. In comparison with raw HIC resin, Octyl@BSA retained a high binding capacity for BSA but rejected other proteins through spatial recognition. The maximum selectivity factors ( α ) of this resin may be close to 5. The adsorption kinetics and frontal adsorption experiments further confirmed the improvement in selectivity in batch and column mode. In addition, the recyclability of Octyl@BSA was tested using ten continuous BSA adsorption–desorption cycles. CONCLUSION This study demonstrated that the introduction of spatial recognition was an effective strategy for enhancing the advantage of target proteins during competitive adsorption, leading to an improvement in selectivity for HIC. © 2020 Society of Chemical Industry
研究领域:
生物化学
·
分离技术
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DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Qun Zeng,Yuzhi Wang,Na Li,Xiu Huang,Xueqin Ding,Xiao Lin,Songyun Huang,Xiaojie Liu
Eight kinds of green ionic liquids were synthesized, and an ionic liquid aqueous two-phase system (ILATPS) based on 1,1,3,3-tetramethylguandine acrylate (TMGA) guanidine ionic liquid was first time studied for the extraction of proteins. Single factor experiments proved that the extraction efficiency of bovine serum albumin (BSA) was influenced by the mass of IL, K 2 HPO 4 and BSA, also related to the separation time and temperature. The optimum conditions were determined through orthogonal experiment by the five factors described above. The results showed that under the optimum conditions, the extraction efficiency could reach up to 99.6243%. The relative standard deviations (RSD) of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.8156% ( n =5), 1.6173% ( n =5) and 1.6292% ( n =5), respectively. UV–vis and FT-IR spectra confirmed that there were no chemical interactions between BSA and ionic liquid in the extraction process, and the conformation of the protein was not changed after extraction. The conductivity, DLS and TEM were combined to investigate the microstructure of the top phase and the possible mechanism for the extraction. The results showed that hydrophobic interaction, hydrogen bonding interaction and the salt out effect played important roles in the transferring process, and the aggregation and embrace phenomenon was the main driving force for the separation. All these results proved that guanidine ionic liquid-based ATPSs have the potential to offer new possibility in the extraction of proteins.
研究领域:
生物化学
·
蛋白质工程
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
·
2022
·Vol. 49
·期 8
·2655-2667
·
IF 10.057
Hou Zhenyu,Zhou Mei,Ma Yuanyuan,Xu Xiaoxuan,Zhang Zhiqi,Lai Shiwei,Fan Wenpei,Xie Jinbing,Ju Shenghong
Purpose Radiation therapy (RT) and photodynamic therapy (PDT) are promising while challenging in treating tumors. The potential radiation resistance of tumor cells and side effects to healthy tissues restrict their clinical treatment efficacy. Effective delivery of therapeutic agents to the deep tumor tissues would be available for tumor-accurate therapy and promising for the tumor therapy. Thus, developing nanoprobes with effectively delivering radiotherapy sensitizers and photosensitizers to the interior of tumors is needed for the accurate combined RT and PDT of tumor. Methods The size-changeable nanoprobes of Gd 2 O 3 @BSA-BSA-Ce6 (BGBC) were synthesized with a crosslinking method. Magnetic resonance imaging (MRI) and in vivo near-infrared (NIR) imaging were measured to evaluate the nanoprobes’ tumor accumulation and intratumor penetration effect. The tumor suppression effect of combined RT and PDT with these nanoprobes was also studied for the 4T1 bearing Balb/c mice. Results The nanoprobes BGBC showed high tumor accumulation and disintegrated into small particles responding to the photo-irradiation-produced reactive oxygen species (ROS), allowing for tumor penetration. Abundant radiotherapy sensitizers and photosensitizers were delivered to the deep tumor tissues, which is available for the accurate therapy of tumor. In addition, the BGBC displayed outstanding MRI and fluorescence imaging effects for evaluating the biodistribution and tumor suppression effect of nanoprobes. Consequently, significant tumor suppression effect was obtained based on the accurate tumor treatment with the combined RT and PDT. Conclusion The designed size-changeable nanoprobes BGBC showed excellent tumor accumulation and deep tumor penetration, resulting in a significant tumor suppression effect based on the combined RT and PDT. This study provides a novel strategy for dual delivery of radiotherapy sensitizers and photosensitizers into the deep tumor tissues and is promising for the accurate theranostics of tumor.
研究领域:
癌症治疗
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放射医学
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纳米医学
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医学影像
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Fengjing Cao,Fu Jiao,Shixiang Ma,Daming Dong
New strategies for super-sensitive detection of toxic heavy-metal-ion pollutants are urgently needed. Here, we outline a simple and highly sensitive assay for copper(II) ions. The method is based on click chemistry and uses laser-induced breakdown spectroscopy (LIBS) as the readout method. Copper(I) ions formed in situ by the reduction of copper(II) ions in the presence of sodium ascorbate (AA) can catalyze the cycloaddition reaction between an alkyne and an azide (CuAAC). In the presence of copper(II) ions and AA , the AgNPs-BSA-Azide can be clicked with the BSA-Alkyne via a cycloaddition reaction to form a BSA-triazole-BSA-AgNPs sandwich conjugate based on this CuAAC&LIBS system. The intensity of the LIBS signal from the residual AgNPs-BSA-Azide in the supernatant is inversely proportional to the concentration of copper(II) ions. The sensor shows a good detection limit of 0.045 nM and a linear relationship between copper ions concentration and the LIBS signal. This click-based method will broaden the potential applications of LIBS for metal detection in drinking water and environment.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
FIBERS AND POLYMERS
·
2018
·Vol. 19
·期 5
·941-948
·
IF 1.353
Li Xiaojie,Chao Guiqun,Wang Liangan,Xu Xianlin,Cai Zhanjun,Shi Lei,Zhuang Xupin,Cheng Bowen
In this work, an affinity nanofiber membrane was successfully prepared by solution blowing of arginine-modified chitosan (CS-Arg) for bovine serum albumin (BSA) adsorption. CS-Arg was firstly synthesized by coupling L-arginine onto chitosan backbone. Then, CS-Arg nanofiber membranes (CANFs) were fabricated using solution blowing process with Polylactide (PLA) as assistant polymer. The results showed that CANFs effectively adsorbed BSA, and the adsorption capacities were influenced by the degrees of substitution (DS) of arginine in CS, pH value, contact time, and initial protein concentration. The highest adsorption capacity of 445.19 mg/g was achieved uvnder the following conditions: DS of 43.7 %, pH of 7.14, and initial concentration of 3.0 mg/ml. BSA adsorbed on the CANFs membrane conformed to Langmuir model, and the adsorption rate was consistent with the second-order kinetics model. This work implies that an arginine-modified chitosan nanofiber-based novel biomaterial has a potential application in adsorption of BSA.
研究领域:
材料科学
·
生物技术
·
化学工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER BULLETIN
·
2022
·Vol. 79
·期 12
·10555-10571
·
IF 2.843
Yu Lili,Yao Lin,Yang Kuan,Fei Wenling,Chen Qingqing,Qin Lan,Liu Shaojing,Cao Min,Liu Qian,Qin Bei
Nano core–shell drug carriers with high stability, low toxicity, and targeted drug delivery are significance for the delivery of anti-tumor drugs. In this study, Albumin from bovine serum (BSA), serving as a capping agent, was conjugated to MSNs via a cleavable disulfide bond to generate a redox-responsive nanocarrier (MSNs@BSA). Subsequently, cRGD peptide, as a targeting ligand, was modified on the particle surface by a protein cross-linker to obtain nanoparticles with tumor cell-targeting properties (RGD-MSNs@BSA). The construction of RGD-MSNs@BSA was confirmed by DLS analysis, scanning electron microscope (SEM), transmission electron microscopy (TEM), electron dispersive spectroscopy (EDS), X-Ray Diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR), respectively. It was displayed that the model anticancer drug doxorubicin (DOX) was efficiently and stably encapsulated in RGD-MSNs@BSA in the absence of glutathione (GSH), and an outbreak of DOX was observed when the particles were exposed to a GSH-containing environment. It demonstrated that disulfide-linked BSA capping can increase the drug loading stability, while enduing it redox sensitivity. Flow cytometry and fluorescence microscope tests displayed that cellular uptake of RGD-MSNs@BSA was much higher than that of particles without cRGD and free DOX. These results indicated that RGD-MSNs@BSA can increase drug tumor-targeting and drug cellular uptake.
研究领域:
纳米医学
·
药理学
·
生物材料科学
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产品关联
DOI
高影响因子
Catalysts
·
2023
·Vol. 13
·期 10
·1320
·
IF 3.9
Yinshan Xie,Xinning Wang,Hulin Li,Tao Wang,Wei Feng,Jian Li
Membrane separation has been widely utilized to eliminate pollutants from wastewater. Among them, a polyacrylonitrile (PAN) ultrafiltration (UF) membrane has presented outstanding stability, and distinguished chemical and thermal properties. However, UF membranes inevitably incur fouling issues during their operation procedure caused by contaminant adhesion on the membrane surface, which would restrict the operational efficiency and increase the maintenance cost. The conventional physical and chemical cleaning is not an effective technique to reduce the fouling due to the additional chemical addition and inevitable structure damage. Recently, UF membranes combined with photocatalytic materials are suggested to be a useful approach to conquer the membrane fouling issues. Herein, TiO2nanoparticles were utilized to blend with a PAN casting solution for fabricating a composite UF membrane via a phase inversion method. With a certain TiO2addition, the obtained membranes presented an enhancement of hydrophilicity, which could promote the water permeability and antifouling performance. The optimized M3 membrane prepared with 15.0 wt% PAN and 0.6 wt% TiO2exhibited an excellent water permeability up to 207.0 L m−2h−1bar−1with an outstanding 99.0% BSA rejection and superior antifouling property. In addition, the photocatalytic TiO2nanoparticles endowed the M3 membrane with a remarkable self-cleaning ability under the UV irradiation. This facile construction method offered new insight to enhance the UF membrane separation performance with an enhanced antifouling ability.Keywords:polyacrylonitrile;ultrafiltration;membrane fouling;TiO2;photocatalysis
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）