FOOD MICROBIOLOGY
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2019
·
IF 4.089
Yunhao Lu,Xinyi Tan,Yuanping Lv,Guohua Yang,Yuanlong Chi,Qiang He
The production of Chinese horse bean-chili-paste (CHCP) involves three fermentation phases: chili-to-moromi fermentation (CF) phase, horse bean-to-meju fermentation (HF) phase and moromi-meju mixed fermentation (MF) phase. To understand the microbial dynamics among these three phases and the potential roles of viable microbes for fermentation, microbial community dynamics was investigated by using culture-dependent and culture-independent methods. Furthermore, the capacities of enzyme-producing of the isolates were determined. During the CF phase, reducing sugar content increased from 3.1% to 3.49%, while pH declined from 4.85 to 4.5. The protein content in the HF phase and MF phase reduced sharply from 22.23% to 10.29% and 4.39%–1.19%, respectively. Bacillus sp., Staphylococcus sp., Oceanobacillus sp., Candida sp., Zygosaccharomyces sp. and Aspergillus sp. dominated the CF phase, while Bacillus sp., Candida sp. and Zygosaccharomyces sp. were the dominant microorganisms in both the HF and MF phases. B. amyloliquefaciens , B. methylotrophicus , B. subtilis, B. licheniformis and A. oryzae possessed strong capacities of producing enzymes, i.e. α -amylase, cellulase and xylanase, acid protease and leucine aminopeptidase , and could make a great contribution to CHCP fermentation.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-11
·
IF 6.408
Xu Yixuan,Shi Xuerong,Ran Fanpeng,Zhang Ziqi,Phipps Josh,Liu Xiaoyan,Zhang Haixia
A novel Tb-doped Eu-based metal–organic framework (Eu-MOF@Tb) has been developed by incorporating hexanuclear europium cluster and 2,2′-bipyridine-5,5′-dicarboxylic acid as well as coordination with Tb(III). Owing to the diverse coordination status of Tb(III) and Eu(III) in MOF, antenna effect emission from Tb(III) can be invoked by dipicolinic acid (DPA), but the luminescence originating from Eu(III) remains unchanged. Taking advantage of this phenomenon, a ratiometric luminescent method for detection of DPA, a biomarker for Bacillus subtilis spores, was developed through differential sensitization toward lanthanide ions. This analysis method allowed for the detection of DPA in the 0.2–10 μM concentration range, with a detection limit of 60 nM. It was further validated by spiked recoveries (89.3–110%) of real-world samples with RSD values in the range 3.9–11%. Alongside this, a paper indicator test was prepared for naked-eye detection of DPA via a dose-sensitive color evolution from red to green under UV light. The effectiveness of the proposed approach was explored in the detection of bacterial spores in real biological and environmental samples and indicated great potential for applications as a real-time monitoring system against the anthrax threat. Graphical Abstract
研究领域:
分析化学
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生物化学
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环境科学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Renlu Liu,Jialiang Zhang,Haiyun Fu,Li Yin,Yongsheng Song,Genhe He
Efficient removal of organic dye pollution from contaminated water is a concern in the absorbent applications. In this study, a green biogenic calcium carbonate (BCC) absorbent was fabricated using Bacillus licheniformis for the removal of methylene blue (MB) from water. This was found to have superior adsorption capacity compared with abiotic calcium carbonate (ACC) and operate within a broad pH range from 3 to 9. MB adsorption on BCC was physical and exothermic. The hydrophobic features, rough nanoporous microstructure, and organic–inorganic mesoporous structure of the BCC may all be responsible for its favorable adsorption mass transfer. The adsorption energy of BCC had a more negative value than that of ACC, indicating a stronger MB interaction with BCC with a lower energy barrier. Hydrogen bonding and electrostatic attraction were involved in the adsorption process. Overall, the findings established a theoretical foundation for the application of BCC in remediation of MB-contaminated water.
研究领域:
环境科学
·
生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Wen Zhang,Mengya Li,Yongqi Yao,Lijun Tan,Haoran Li,Liangmin Wei,Baocai Xu
Bacillus species form highly heat-resistant spores, causing food spoilage and economic losses in the food industry. This study employed a “germinate and inactivate” strategy to effectively inactivate Bacillus altitudinis and Bacillus safensis spores. We first prepared curcumin-thymol nanoparticles (CT NPs) to mediate photodynamic inactivation (PDI) of vegetative cells under white light. Subsequently, optimal synergistic parameters were determined. Combining 5 mM L-aspartic (Asp) and 50 μM CT NPs with 65 °C thermal treatment under white light (TL-Asp) for 120 min achieved log reductions of 3.75 for B. altitudinis spores and 4.05 for B. safensis spores. Multiparameter analysis, including pyridine-2,6-dicarboxylic acid (DPA) release, nucleic acid and protein leakage, Na + /K + -ATPase activity, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and heat resistance, provided mechanistic insights into spore inactivation. Specifically, germinant reduced spore resistance, while 65 °C thermal treatment enhanced photodynamic penetration into spore inner membrane (IM) and core, thereby exacerbating IM damage and nucleic acid leakage, leading to synergistic inactivation. Additionally, 65 °C heat synergistically promoted core hydration by increasing membrane fluidity, which accelerated spore germination. These findings suggest that TL-Asp treatments hold potential for controlling spores in food industry applications.
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PubMed
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Yang Liu,Wenwen Ge,Yuxin Sun,Hongchao Dai,Luyao Fan,Lei Yuan,Zhenquan Yang,Xinan Jiao
Bacillus cereus and Bacillus licheniformis are widely presented in dairy products. They can form thick biofilms on surfaces of dairy processing equipment, which may pose serious safety issues and spoilage of final dairy products. However, how ecological interactions between B. cereus and B. licheniformis affect the functions and stability of mixed-species biofilm remains uncovered. In this work, the altered profiles of a dual-species biofilm by dairy-derived B. cereus 121 and B. licheniformis 919 were investigated by RNA-sequencing analysis in combined with phenotype validation (bacterial growth, biofilm-forming capacity, biofilm EPS production, and biofilm structures). The results confirmed that the presence of B. cereus 121 reduced the growth of B. licheniformis 919 planktonic cells, and decreased the biofilm cell numbers of B. licheniformis 919 in the dual-species biofilm when compared to that in its single-species biofilm. The bacterial interaction also reduced the amount of proteins and carbohydrates in the biofilm matrix, and decreased the coverage, average thickness, and total biomass of biofilms. In addition, results from RNA-sequencing analysis showed that the bacterial interaction caused a total of 128 ( B. licheniformis 919) and 216 ( B. cereus 121) differentially expressed genes (DEGs) during the co-culture of planktonic cells. Functional annotation revealed that the DEGs of B. licheniformis 919 were mainly involved in 10 downregulated pathways including citrate cycle, pyruvate metabolism, nonribosomal peptide structures, glycolysis/gluconeogenesis, quorum sensing, alanine, aspartate and glutamate metabolism, oxidative phosphorylation, beta-Lactam resistance, arginine and proline metabolism, and beta-Alanine metabolism when co-cultured with B. cereus 121. On the other hand, the DEGs from B. cereus 121 were significantly enriched for two downregulated pathways (cysteine and methionine metabolism, and inositol phosphate metabolism) and four upregulated pathways (nitrogen metabolism, glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, and propanoate metabolism). Results of this study facilitate updated knowledge of how bacterial interaction during the biofilm formation shapes the features of the mixed-species biofilm.
研究领域:
微生物学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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2013
·
IF 2.823
Long-Hua Zhou,Na Wang,Wei Zhang,Zong-Bo Xie,Xiao-Qi Yu
An facile and green one-pot route has been developed for the synthesis of chromenes using salicylaldehyde and α,β-unsaturated ketones. α-Amylase from Bacillus subtilis shows excellent catalytic activity and exerts good adaptability to different substrates in the reaction. This promiscuous enzyme-catalyzed domino reaction not only extends the application of α-amylase from B. subtilis for new chemical transformations, but also provided an alternative synthetic method for 2 H -chromene derivatives.
研究领域:
生物化学
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有机合成
·
酶学
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DOI
高影响因子
ACS ES&T Engineering
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2025
·
IF 6.7
Zulv Huang,Xipeng Wei,Lijuan Zhang,Tao Chen,Yi Huang,Zhangwei Yang,Wanqing Zeng,Hongxing Chen,Lingtian Xie,Bo Yan
Microbial reduction of pentavalent vanadium [V(V)] to less toxic tetravalent vanadium [V(IV)] is associated with the biogeochemical cycling of sulfur. However, the capability of elemental sulfur-reducing bacteria (S0RB) to reduce V(V) in the presence of elemental sulfur (S0) remains largely unexplored. Herein, we systematically investigated the V(V) reduction capabilities and underlying mechanisms of Bacillus megaterium R01, a S0RB strain isolated from the stone coal mining region. Under sulfur-depleted conditions, B. megaterium R01 demonstrated limited reduction capacity (<54.3% removal efficiency for 200 mg/L V(V)). In contrast, S0 supplementation enabled near-complete V(V) reduction (97.6 ± 2.1%) even at elevated concentrations (400 mg/L), achieving a relatively low carbon consumption rate (TOC/V(V) of ∼0.6). Sulfhydryl (SH) compounds, reaching 424.2 ± 9.9 μmol/L within 5 days, were likely crucial for reducing V(V) to V(IV). This mechanism differs from that of other V-reducing bacteria. The transcriptome analysis revealed that the up-regulation of genes associated with sulfur metabolism, cysteine, and methionine metabolism in B. megaterium R01 is likely to contribute to its enhanced resistance toward V(V). Additionally, the biosynthesis of intracellular BSH in B. megaterium R01, and the extracellular SH groups (e.g., Trx and TrxR encode enzymes) play an irreplaceable role in reducing V(V) by B. megaterium R01. This study enhances our understanding of V(V) reduction in sulfur-rich environments and suggests a promising approach for remediating V(V)-contaminated groundwater, sediments, and soils.
研究领域:
环境微生物学
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污染修复
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生物地球化学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2019
·
IF 3.927
Li Zheng,Jun-jian Situ,Qing-feng Zhu,Ping-gen Xi,Yin Zheng,Hong-xia Liu,Xiaofan Zhou,Zi-de Jiang
Litchi is an important economic fruit in subtropical countries. The litchi downy blight (LDB) caused by the oomycete Peronophythora litchii severely affects the production and quality of litchi fruit, and is widespread in almost all litchi production regions of China. Therefore, there is an urgent need for effective and sustainable control strategies against LDB. Our previous study showed that Bacillus amyloliquefaciens LI24 and PP19, B. licheniformis HS10, B. pumilus PI26, and Exiguobacterium acetylicum SI17 are promising biocontrol agents (BCAs) in controlling LDB, and their volatile organic compounds (VOCs) could inhibit the growth of P. litchii in vitro . In this study, we found that pre-exposure of litchi fruit to VOCs produced by PP19, SI17 and PI26 can significantly reduce the severity of LDB during 36 h to 72 h post inoculation. We further analyzed VOCs produced from the three BCAs ( i.e. , PP19, SI17, PI26) by solid phase microextraction gas chromatography-mass spectrometry (SPME-GC–MS), and found that their chemical compositions varied substantially over incubation time and between BCAs. In total, 70, 98, 101 chemicals were detected in PP19, SI17, PI26 from 24 to 72 h of incubation, respectively; 17 of them were commonly produced at more than one time points by PP19, and 11 were selected for further study. Two of the compounds 1-(2-Aminophenyl)ethanone (EA) and Benzothiazole (BTH) showed inhibitory activity against both P. litchii on plates and LDB on litchi fruit when the compounds were directly applied, while another compound α-Farnesene (AF) was able to suppress LDB in vivo , but did not exhibit antagonistic activity against the pathogen in vitro , suggesting that it may act through induction of host defense mechanisms. Our results showed that the bacterial VOCs and compounds of BTH or AF could be promising for the control of LDB on harvested litchi fruit.
研究领域:
植物病理学
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微生物学
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DOI
高影响因子
iScience
·
2023
·
IF 5.8
Qilin Yu,Haohao Mao,Bowen Yang,Yahui Zhu,Cheng Sun,Zhiqiang Zhao,Yang Li,Yaobin Zhang
Electrical stimulation has been used to strengthen microbial extracellular electron transfer (EET), however, the deep-seated reasons remain unclear. Here we reported that Bacillus subtilis , a typical gram-positive bacterium capable of extracellular respiration, obtained a higher EET capacity after the electrical domestication. After the electrical domestication, the current generated by the EET of B. subtilis was 23.4-fold that of the control group without pre-domestication. Multiple lines of evidence in bacterial cells of B. subtilis , their cell walls, and a model tripeptide indicated that the polarization of amide groups after the electrical stimulation forwarded the H-bonds recombination and radical generation of protein-like substances to develop extracellular electron transfer via the proton-coupled pattern. The improved electrochemical properties of protein-like substances benefited the trans -cell-wall electron transfer and strengthen extracellular respiration. This study was the first exploration to promote microbial extracellular respiration by improving the electrochemical properties of protein-like substances in cell envelopes.
研究领域:
微生物学
·
生物物理学
·
电化学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·Vol. 195
·期 12
·7553-7567
·
IF 3
Li Xiaozheng,Jiang Junpo,Li Xinyue,Liu Dexu,Han Mengnan,Li Wei,Zhang Honglei
An efficient cofactor regeneration system has been developed to provide a hydride source for the preparation of optically pure alcohols by carbonyl reductase-catalyzed asymmetric reduction. This system employed a novel glucose dehydrogenase (BcGDH90) from Bacillus cereus HBL-AI. The gene encoding BcGDH90 was found through the genome-wide functional annotation. Homology-built model study revealed that BcGDH90 was a homo-tetramer, and each subunit was composed of βD-αE-αF-αG-βG motif, which was responsible for substrate binding and tetramer formation. The gene of BcGDH90 was cloned and expressed in Escherichia coli. The recombinant BcGDH90 exhibited maximum activity of 45.3 U/mg at pH 9.0 and 40 °C. BcGDH90 showed high stability in a wide pH range of 4.0–10.0 and was stable after the incubation at 55 °C for 5 h. BcGDH90 was not a metal ion-dependent enzyme, but Zn 2+ could seriously inhibit its activity. BcGDH90 displayed excellent tolerance to 90% of acetone, methanol, ethanol, n-propanol, and isopropanol. Furthermore, BcGDH90 was applied to regenerate NADPH for the asymmetric biosynthesis of ( S )-(+)-1-phenyl-1,2-ethanediol (( S )-PED) from hydroxyacetophenone (2-HAP) with high concentration, which increased the final efficiency by 59.4%. These results suggest that BcGDH90 is potentially useful for coenzyme regeneration in the biological reduction.
研究领域:
生物化学
·
酶工程
·
合成生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Qianyu Xu,Xiaoyu Lin,Li Gan,Gary Owens,Zuliang Chen
While chemical methods are often used to convert graphene oxide (GO) to reduced graphene oxide (RGO), chemical reduction is often environmentally unfriendly due to the high toxicity of many chemical reducing agents. To address this limitation, Bacillus sphaericus was used here for the green reduction of GO to RGO. Successful reduction was confirmed by various advanced characterization techniques including Ultraviolet–Visible (UV–vis), X-ray Powder Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, X-ray Photoelectron Spectroscopy (XPS) and Scanning Electron Microscope and Energy Dispersive Spectrometer (SEM-EDS). With a new peak attributable to RGO at 261 nm appearing in UV–vis and XRD spectra of the reduced product also developed a new peak at 2θ = 24.6° characteristic of RGO. Successful reduction was also supported by Raman spectroscopy which showed that the ratio of the intensity band (D band: G band) increased from 0.99 to 1.17. FTIR and XPS both confirmed that specific O H (3399 cm −1 ), C O (1734 cm −1 ) and C O C (287 eV) bonds were reduced. Cyclic voltammograms (CVs) showed that the produced RGO exhibited good conductivity (changed from 0.8 to 1.1 mW·cm −2 ). This work developed a green and easy operated method of synthesizing RGO using microorganisms.
研究领域:
材料科学
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环境科学
·
微生物学
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DOI
PubMed
高影响因子
Microorganisms
·
2023
·Vol. 11
·期 8
·1989
·
IF 4.5
Xiaoyu Cao,Jingyuan Cai,Yu Zhang,Chao Liu,Meijie Song,Qianqian Xu,Yang Liu,Hai Yan
High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was successfully isolated fromBaijiucellar mud using UA as the sole carbon and energy source. The bacterial strain was identified asBacillus paramycoides-YC02 through 16S rDNA sequence analysis. Under optimal culture conditions at an initial pH of 7.0 and 38 °C, YC02 completely biodegraded an initial UA concentration of 500 mg/L within 48 h. Furthermore, cell-free extracts of YC02 were found to catalyze and remove UA. These results demonstrate the strong biodegradation ability of YC02 toward UA. To gain further insight into the mechanisms underlying UA biodegradation by YC02, the draft genome of YC02 was sequenced using Illumina HiSeq. Subsequent analysis revealed the presence of gene1779 and gene2008, which encode for riboflavin kinase, flavin mononucleotide adenylyl transferase, and flavin adenine dinucleotide (FAD)-dependent urate hydroxylase. This annotation was based on GO or the KEEG database. These enzymes play a crucial role in the metabolism pathway, converting vitamin B2to FAD and subsequently converting UA to 5-hydroxyisourate (HIU) with the assistance of FAD. Notably, HIU undergoes a slow non-enzymatic breakdown into 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) and (S)-allantoin. The findings of this study provide valuable insights into the metabolism pathway of UA biodegradation byB. paramycoides-YC02 and offer a potential avenue for the development of bacterioactive drugs against HUA and gout.Keywords:uric acid;Bacillus paramycoides-YC02;biodegradation;genomic analysis
研究领域:
微生物学
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基因组学
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代谢研究
·
生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）