TALANTA
·
2018
·
IF 4.244
Ke Li Lin,Tong Yang,Hong Yan Zou,Yuan Fang Li,Cheng Zhi Huang
Here we first reported that graphitic carbon nitride nanosheet (g-C 3 N 4 NS) could effectively quench the chemiluminescence (CL) of luminol-hydrogen peroxide (H 2 O 2 ) system. According to the new discovery, a label-free and homogeneous CL aptasensing platform was designed for sensitive detecting of biomarkers. In the absence of target, DNA probe containing hemin/G-quadruplex DNAzyme structure was adsorbed on the surface of g-C 3 N 4 NS, causing the CL quenching of luminol through an electron transfer process. However, in the presence of the target, a DNA-DNA duplex was formed due to DNA hybridization reaction and target recognition effect, which could not be adsorbed onto the g-C 3 N 4 NS surface because of its weak affinity. Thus, the electron transfer was blocked and the CL emission of luminol could be enhanced. The proposed CL aptasensor could detect carcinoembryonic antigen (CEA) with a detection limit of 63.0 pg/mL and it can also be used as a general detecting strategy for adenosinetriphosphate (ATP) detection. This aptasensing platform exhibited high sensitivity toward biomarkers and the probe need not be labeled, showing great promise for disease diagnosis.
研究领域:
生物化学
·
分析化学
·
医学诊断
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DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yan Wang,Jin Shen,Ruilong Song,Qin Xu,Xiaoya Hu,Yun Shu
Rapid and highly sensitive detection of tumor marker (TM) is critical for the early diagnosis and treatment of cancers. Herein, utilizing highly bright and water-stable CsPbBr 3 perovskite nanocrystals (NCs) capped with amphiphilic polymer ligand of octylamine-modified polyacrylic acid (OPA) and gold nanoparticles (AuNPs) as reporters, a lateral flow immunoassay (LFIA) strip is developed for fluorescence and colorimetric dual-mode detection of carcinoembryonic antigen (CEA). The prepared CsPbBr 3 NCs capped by an amphiphilic polymeric of OPA ligand showed high stability and bright fluorescence. Moreover, the AuNPs immunoprobes were captured with CEA antigen and quench the green fluorescence of CsPbBr 3 /OPA NCs on the T line due to the inner filter effect (IFE). Therefore, CEA could be quantitative analyzed by the dual-readout of fluorescence and colorimetric signal. The detection limits of CEA can reach as low as 0.023 ng/mL and 0.027 ng/mL for the fluorescence and colorimetric mode, respectively. Good specificity and reproducibility were also demonstrated for this method. Finally, the CsPbBr3/OPA NCs-based LFIA showed good accuracy in detection of CEA level from clinical serum samples. This work firstly enables the application of CsPbBr 3 perovskite NCs in a LFIA, displaying great potential in point-of-care clinical diagnosis.
研究领域:
生物医学工程
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肿瘤学
·
纳米医学
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DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Bing Zhang,Xue Wang,Yanling Zhao,Jia Lv,Hongyun Meng,Honghong Chang,Hui Zhang,Wenlong Wei
Visual detection of tumor markers with accuracy and selectivity exhibits significant advantages for cancer early diagnosis due to its low-cost and simple analytical instruments. Herein, a highly photosensitive colorimetric immunoassay for carcino embryonie antigen (CEA, as a model) detection based on Cu 2+ doped Ag-AgI nanocomposite was constructed. First, Cu 2+ doped Ag-AgI nanocomposite (Cu 2+ /Ag-AgI) owned the ability of oxidization of TMB was synthesized by impregnation method, which was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), UV-Vis diffuse reflectance spectroscopy (DRS) and X-ray photoelectron spectroscopy (XPS). Second, based on sandwich-type immunoassay, the immunocomplex was built on the surface of magnetic bead by utilizing Cu 2+ /Ag-AgI as labels. The blue color of TMB ox was obtained under visible irradiation and ultraviolet spectrum scanning was carried out in ABS buffer solution. Under optimal conditions, the absorbance values increased with the increasing of CEA concentrations in sample with the linearity range of 1.0 pg mL −1 −5.0 ng mL −1 . The detection limit was 0.13 pg mL −1 . More importantly, the developed colorimetric immunoassay exhibited good selectivity, repeatability, stability and possible applications in the real serum sample analysis.
研究领域:
生物医学工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Yuchen Jin,Qi Kang,Xinli Guo,Bin Zhang,Dazhong Shen,Guizheng Zou
Signal amplification for electrochemiluminescence (ECL) has conventionally been achieved by employing effective matrixes that can accelerate the electrochemical redox processes or carry more electrochemiluminophores. Herein, a convenient signal-amplification strategy was proposed for an ECL immunoassay with carboxylated g-C3N4 nanosheets (NSs) as tags and carcinoembryonic antigen (CEA) as the model target via electrochemically pretreating the substrate: a glassy-carbon electrode (GCE) modified with a polymerized 2-aminoterephthalic acid (ATA) film (GCE/ATA). Bioconjugates of g-C3N4 NSs and the signal CEA antibody (Ab2) (i.e., g-C3N4 NS–Ab2) were immobilized on GCE/ATA via a sandwich immunoreaction to form GCE/ATA–Ab1–Ag–Ab2–NSs. Electrochemical-impedance spectroscopy and potential-resolved ECL characterization proved that GCE/ATA plays an important role in the electron-transfer resistance (Ret) of the GCE/ATA–Ab1–Ag–Ab2–NSs for ECL and that successively scanning GCE/ATA–Ab1–Ag–Ab2–NSs from 0 to −1.6 V in K2S2O8- and H2O2-containing medium could reduce the Ret and bring out 3.3-times-enhanced ECL at the 10th scan cycle compared with that of the 1st scan cycle, which was about 10.2 times the ECL of the GCE/ATA–Ab1–Ag–Ab2–NSs in medium containing merely K2S2O8. Inspired by this, direct and successive scanning of GCE/ATA in K2S2O8- and H2O2-containing medium was employed during fabrication, which dramatically reduced the Ret of GCE/ATA–Ab1–Ag–Ab2–NSs and brought out obviously enhanced ECL responses for selectively determining CEA from 0.1 pg/mL to 1 ng/mL, with a detection limit of 3 fg/mL.
研究领域:
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-9
·
IF 5.3
Xiang Jiawang,Zhang Bing,Yuan Yuan,Zhao Zhihuan,Lin Jianying,Li Jing
A novel signal amplification strategy was developed by combining near-infrared light with MoS 2 /CuO/Au nanocomposite for building a colorimetric immunoassay. First, MoS 2 /CuO/Au nanocomposite was synthesized by precipitation and photoreduction methods and characterized by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD). MoS 2 /CuO/Au nanocomposite has oxidase-like activity and can oxidize TMB to form a blue product (TMBox). Further, the catalytic oxidation of TMB was accelerated under near-infrared (NIR) laser radiation. The sandwich-type colorimetric immunoassay was constructed using MoS 2 /CuO/Au nanocomposite. Under the enhancement of near-infrared light, carcinoembryonic antigen (CEA) was sensitively detected in the range 0.1 to 40 ng/mL with the limit of detection of 0.03 ng/mL. Moreover, the immunosensor has excellent selectivity and anti-interference, good repeatability, and stability. Graphical Abstract
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Yejing Jia,Bing Zhang,Honghong Chang,Feiyan Yu,Zhihuan Zhao
A simple colorimetric immunoassay based on visible light excitation for detection of carcinoembryonic antigen (CEA) was developed. Firstly, visible light photocatalysts of TiO 2 /SnO x -Au ternary heterostructures were prepared by in situ reduction method, which were characterized using transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD) and UV–vis diffuse reflectance spectroscopy (DRS). Then, a novel sandwich-type colorimetric immunoassay for quantitative analysis of CEA by using TiO 2 /SnO x -Au nanoparticle as signal tag was constructed. Under visible light irradiation (λ ≥ 420 nm), the color development of TMB was obtained. And the quantitative analysis was carried out in ABS buffer solution by ultraviolet spectrum scanning. Under optimal conditions, the absorbance values increased with the increasing of CEA levels in samples, which presented linear relationship in the range of 5 pg mL −1 –2.5 ng mL −1 with a detection limit of 5 pg mL -1 . Meanwhile, the colorimetric immunosensor owned acceptable specificity, stability and reproducibility.
研究领域:
生物医学工程
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免疫学
·
材料科学
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DOI
PubMed
高影响因子
ACS Nano
·
2023
·
IF 17.1
Xiaoming Su,Xinyu Liu,Yangcenzi Xie,Mingyang Chen,Chao Zheng,Hong Zhong,Ming Li
Protein profiles of exosomes (EXOs) in clinical samples of cancer patients have become a promising diagnostic and therapeutic biomarker. However, simultaneous quantitative analysis of multiple exosomal proteins of interest remains challenging. To address the unmet need, we develop a paper-based surface-enhanced Raman spectroscopy (SERS)-vertical flow biosensor, named iREX (integrated Raman spectroscopic EXO) biosensor, for multiplexed quantitative profiling of exosomal proteins in clinical serum samples of patients. Utilizing this iREX biosensor, we are able to quantitatively profile MUC1, HER2 and CEA in EXO samples derived from various breast cancer cell subtypes. The results show discriminative expression profiles of the three exosomal proteins in these cell subtypes, which allows for accurate diagnosis and molecular subtyping of breast cancer. We further validate the clinical utility of the iREX biosensor for simultaneous quantitative analysis of MUC1, HER2 and CEA in patient’s blood serums, thereby aiding in noninvasive breast cancer subtyping and longitudinal treatment monitoring. Our iREX biosensor integrating the SERS detection in a vertical flow diagnostic device offers great advantages of high sensitivity, molecular specificity, powerful multiplexing capability, and high diagnostic accuracy. We believe that the iREX biosensor could be a promising clinical tool for comprehensive analysis of exosomal proteins in clinical samples for personalized diagnosis and precise management of breast cancer.
研究领域:
生物医学工程
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肿瘤学
·
诊断学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Xue Wang,Bing Zhang,Jing Li,Honghong Chang,Wenlong Wei
Colorimetric assay is a powerful tool for detection of tumor markers with the outstanding advantages of visualization and simple analytical instruments. Herein, a simple and fast chromogenic reaction was developed based on Ag 3 PO 4 /Ag nanocomposite synthesized by one-step chemical bath method. And the Ag 3 PO 4 /Ag nanocomposite owned the ability of oxidizing of TMB under the acidic condition due to the hydrolytic action. The nanocomposite was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Then, the colorimetric immunoassay was constructed by utilizing Ag 3 PO 4 /Ag nanocomposite as molecular labels for carcino embryonie antigen (CEA, UniProt accession number: P06731) detection. The blue color with gradient change was observed with the increasing of CEA concentrations. Under optimal conditions, the variation between absorbance values and CEA levels was linear in the range of 0.2 ng mL −1 ~ 2.5 ng mL −1 with the detection limit of 0.03 ng mL −1 . More importantly, the developed colorimetric immunoassay exhibited good selectivity , repeatability, stability and potential application in the real serum sample detection.
研究领域:
分析化学
·
生物医学工程
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2026
·Vol. 16
·期 6
·303
·
IF 6.2
Yu Chen,Lina Dong,Hengyan Tian,Fei Yang,Dengbang Jiang,Minglong Yuan
Magnetic polymeric microspheres are pivotal solid-phase carriers in chemiluminescence enzyme immunoassays (CLEIA). However, their practical clinical application is frequently hindered by non-specific adsorption, irreversible aggregation, and the intrinsic susceptibility of exposed outermost Fe3O4nanoparticles to oxidation. To overcome these critical bottlenecks, we rationally engineered highly original monodisperseP(St-MMA)@Fe3O4@P(St-MMA) sandwich-structured microspheres. The bespoke amphiphilic outer shell acts as an impenetrable shield against hydration and oxidation, while maintaining a topologically size-matched mesoporous network (average pore size of 13.11 nm) for optimal antibody anchoring. Strikingly, this architecture ensures exceptional long-term colloidal stability, completely preventing macroscopic agglomeration for over six months in buffer solutions. When evaluated in a carcinoembryonic antigen (CEA), CLEIA, our microspheres achieved an ultra-low limit of detection (LOD) of 0.055 ng·mL−1and high analytical recovery (93.37–108.25%). In a head-to-head comparison with industry-standard commercial magnetic beads, the engineered microspheres delivered stronger chemiluminescent signals and lower background noise, demonstrating excellent intra-assay (CV < 4.37%) and inter-assay (CV < 10%) precision. This work establishes a scalable, highly stable materials platform that effectively resolves the persistent oxidation limitations, holding immense practical importance for next-generation ultrasensitive clinical in vitro diagnostics.
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DOI
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 1
·103
·
IF 5.4
Xu Li,Jiali Huang,Jiayu Ding,Mingzhen Xiu,Kang Huang,Kang Cui,Jing Zhang,Shiji Hao,Yan Zhang,Jinghua Yu,Yizhong Huang
A dual-mode lab-on-paper device based on BiVO4/FeOOH nanocomposites as an efficient generating photoelectrochemical (PEC)/colorimetric signal reporter has been successfully constructed by integration of the lab-on-paper sensing platform and PEC/colorimetric detection technologies for sensitive detection of carcinoembryonic antigen (CEA). Concretely, the BiVO4/FeOOH nanocomposites were in situ synthesized onto the paper-working electrode (PWE) through hydrothermal synthesis of the BiVO4layer on cellulose fibers (paper-based BiVO4) which were initially modified by Au nanoparticles for improving the conductivity of three dimensional PWE, and then the photo-electrodeposition of FeOOH onto the paper-based BiVO4to construct the paper-based BiVO4/FeOOH for the portable dual-mode lab-on-paper device. The obtained nanocomposites with an FeOOH needle-like structure deposited on the BiVO4layer exhibits enhanced PEC response activity due to its effective separation of the electron–hole pair which could further accelerate the PEC conversion efficiency during the sensing process. With the introduction of CEA targets onto the surface of nanocomposite-modified PWE assisted by the interaction with the CEA antibody from a specific recognition property, a signal-off PEC signal state with a remarkable photocurrent response decreasing trend can be achieved, realizing the quantitative detection of CEA with the PEC signal readout mode. By means of a smart origami paper folding, the colorimetric signal readout is achieved by catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxidized TMB in the presence of H2O2due to the satisfied enzyme-like catalytic activity of the needle-like structure, FeOOH, thereby achieving the dual-mode signal readout system for the proposed lab-on-paper device. Under the optimal conditions, the PEC and colorimetric signals measurement were effectively carried out, and the corresponding linear ranges were 0.001–200 ng·mL−1and 0.5–100 ng·mL−1separately, with the limit of detection of 0.0008 and 0.013 ng·mL−1for each dual-mode. The prepared lab-on-paper device also presented a successful application in serum samples for the detection of CEA, providing a potential pathway for the sensitive detection of target biomarkers in clinical application.Keywords:PEC;colorimetric;lab-on-paper device;BiVO4/FeOOH;CEA
研究领域:
生物医学工程
·
分析化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 38
·6626-6633
·
IF 0
Min Liu,Yuhang Ye,Cheng Yao,Wenbo Zhao,Xiaohua Huang
Chemiluminescence was used as an excitation light source to construct a universal photoelectrochemical platform based on Mn2+-doped NaYF4:Yb/Er upconversion nanoparticles, which greatly improves the electrochemiluminescence intensity and offers more stable cathodic signals compared to pure NaYF4:Yb/Er NPs. Here, we report for the first time the ECL behaviors of Mn2+-doped NaYF4:Yb/Er NPs, which were synthesized via a facile strategy. Mn2+ doping resulted in a 4-fold ECL intensity enhancement of NaYF4:Yb/Er. The characteristics of Mn2+-doped NaYF4:Yb/Er nanocomposites were obtained using transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDS), and fluorescence spectra. After all the results had indicated that NaYF4:Yb/Er upconversion nanoparticles were successfully doped with Mn2+, the electrochemiluminescence platform was built. The as-prepared NaYF4:Yb/Er NPs were rendered water-soluble and then employed as ECL emitters for carcinoembryonic antigen (CEA) determination. The results indicated that the modified electrode can be used to determine CEA without interference from non-specific proteins, with a low detection limit of 5.2 pg mL−1. The biosensor can also be used for quantification of the CEA concentration in real samples.
研究领域:
生物医学工程
·
纳米医学
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DOI
PubMed
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-9
·
IF 5.705
Wang Jiasheng,Hui Ni
A non-fouling electrochemical immunosensor is described for determination of the tumor biomarker carcinoembryonic antigen (CEA). It is based on the use of composite wires made by chemical grafting of hyaluronic acid onto polyaniline nanowires. The modified nanowires possess excellent antifouling property both in single protein solutions and in dilute serum samples. The current of immunoelectrode exhibits a linear response in the 0.01 pg mL −1 to 10,000 pg mL −1 CEA concentration range and 0.0075 pg mL −1 detection limit. This work demonstrates that coating an electrode with hyaluronic acid can largely reduce unspecific adsorption of proteins on the electrode surface.
Schematic of a nonfouling electrochemical immunosensor for the carcinoembryonic antigen. It is based on novel composite wires made through the chemical grafting of easily available hyaluronic acid (HA) onto polyaniline (PANI) nanowires. The HA/PANI demonstrated excellent antifouling property both in single protein solutions and human serum samples.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）